FastQCFastQC Report
Thu 26 May 2016
SRR938526_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938526_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141294
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96820.4521564997613593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75130.35086260924469037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51320.23966816326949966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51700.043.5674061
GTATCAA90950.039.792571
TCAACGC115150.030.8314654
TATCAAC116000.030.8096832
CAACGCA117400.030.2398665
AACGCAG120500.029.4951466
ATCAACG122950.028.914163
GTACATG95250.026.3973311
ACGCAGA135350.026.1857997
CGCAGAG135750.026.0386438
TACATGG100100.025.1159112
GCAGAGT142100.024.2058099
ACATGGG101650.024.0321833
CATGGGT27750.022.2647534
GAGTACT81850.022.1981512-13
AGTACTT86000.021.92785512-13
ACCGCAT1302.93846E-421.9277028
GTATAGG7100.021.462621
TAGCCCT20500.021.3290484
CTACACG3800.021.2619364
Produced by FastQC (version 0.11.5)