##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938526_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2141294 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.172181867599686 31.0 31.0 34.0 28.0 34.0 2 31.292617454679274 31.0 31.0 34.0 28.0 34.0 3 31.348406150673377 31.0 31.0 34.0 28.0 34.0 4 34.98288558226941 37.0 35.0 37.0 32.0 37.0 5 35.02410178144617 37.0 35.0 37.0 32.0 37.0 6 35.06146423611143 37.0 35.0 37.0 32.0 37.0 7 35.00287116108297 37.0 35.0 37.0 32.0 37.0 8 34.97267353291981 37.0 35.0 37.0 32.0 37.0 9 36.49203238789256 39.0 37.0 39.0 32.0 39.0 10-11 36.35162710024873 39.0 35.0 39.0 32.0 39.0 12-13 36.246767141737664 39.0 35.0 39.0 32.0 39.0 14-15 37.24624199199176 39.0 36.5 41.0 31.5 41.0 16-17 37.13122228895239 39.0 36.0 41.0 31.0 41.0 18-19 37.1833438098645 39.5 36.5 41.0 31.0 41.0 20-21 37.30321455157489 40.0 37.0 41.0 31.0 41.0 22-23 37.22050918743526 39.0 36.5 41.0 31.0 41.0 24-25 37.144573561594065 39.0 36.0 41.0 31.0 41.0 26-27 36.98971322947713 39.0 36.0 41.0 31.0 41.0 28-29 36.820085891988676 39.0 36.0 41.0 30.0 41.0 30-31 36.62405746245028 39.0 36.0 40.5 30.0 41.0 32-33 36.364732260025946 39.0 35.0 40.0 30.0 41.0 34-35 36.149733992623155 38.5 35.0 40.0 30.0 41.0 36-37 35.900392239458945 38.0 35.0 40.0 29.0 41.0 38-39 35.67695795159376 38.0 35.0 40.0 27.5 41.0 40-41 35.612215791012346 38.0 35.0 40.0 27.0 41.0 42-43 35.452596187165334 38.0 35.0 40.0 27.0 41.0 44-45 35.14038287129185 38.0 34.0 40.0 25.5 41.0 46-47 34.743583786252614 38.0 34.0 40.0 24.0 41.0 48-49 34.38370840248933 38.0 33.0 40.0 23.5 41.0 50-51 33.67392076940392 37.5 33.0 39.5 22.5 40.5 52-53 33.78446140511298 38.0 33.0 39.5 23.0 40.5 54-55 33.977364621579284 38.0 33.0 40.0 22.0 41.0 56-57 33.75058983026152 38.0 33.0 40.0 20.0 41.0 58-59 33.48863631056735 38.0 33.0 40.0 18.5 41.0 60-61 33.13643712633576 37.5 32.0 40.0 16.5 41.0 62-63 32.681353891618805 37.0 31.5 40.0 13.0 41.0 64-65 32.2659254170609 36.5 31.0 39.0 8.5 41.0 66-67 31.778677052287073 36.0 31.0 39.0 7.5 40.0 68-69 31.25585370341485 35.5 30.0 39.0 4.5 40.0 70-71 30.687981426184354 35.0 29.0 38.0 2.0 40.0 72-73 30.15597274358402 34.5 29.0 37.5 2.0 39.5 74-75 29.577247916446783 34.0 28.0 37.0 2.0 39.0 76-77 28.855030649691262 34.0 27.0 36.0 2.0 39.0 78-79 28.0924606803176 33.5 26.0 36.0 2.0 38.5 80-81 27.434844537928935 33.0 26.0 35.0 2.0 37.0 82-83 26.769121615247602 32.5 24.5 35.0 2.0 37.0 84-85 26.104508535493025 32.0 22.5 35.0 2.0 36.0 86-87 25.431479983598702 31.5 20.5 34.5 2.0 36.0 88-89 24.63723173931277 31.0 17.5 34.0 2.0 35.0 90-91 23.80515426653229 31.0 8.5 34.0 2.0 35.0 92-93 22.976149234995287 30.0 2.0 34.0 2.0 35.0 94-95 22.015092042475253 29.5 2.0 34.0 2.0 35.0 96-97 20.75488092714032 29.0 2.0 34.0 2.0 35.0 98-99 19.26915080320591 26.5 2.0 34.0 2.0 35.0 100-101 15.699704010752377 13.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7285932430176629 1101 2 0.6704463465221764 1101 3 0.747737352804517 1101 4 0.6532722577169139 1101 5 0.6300624059661999 1101 6 0.7611335294318025 1101 7 0.6646745091239765 1101 8 0.6955057916754868 1101 9 0.690296539010717 1101 10-11 0.7316074246182751 1101 12-13 0.7529174384922044 1101 14-15 0.8723239355161283 1101 16-17 0.8637678720991104 1101 18-19 0.956346738793016 1101 20-21 1.0416200411116492 1101 22-23 1.0071396677145614 1101 24-25 1.194478487917678 1101 26-27 1.1472391401471782 1101 28-29 1.1628417906210515 1101 30-31 1.11743969736019 1101 32-33 1.1392793721215781 1101 34-35 1.29143230394439 1101 36-37 1.3263770680059679 1101 38-39 1.257299559057934 1101 40-41 1.3648522709320403 1101 42-43 1.336781190218467 1101 44-45 1.437162631022268 1101 46-47 1.5620046957673779 1101 48-49 1.4751800992280337 1101 50-51 1.3602726835016483 1101 52-53 1.3527083423615451 1101 54-55 1.5022662017928141 1101 56-57 1.2876092033949362 1101 58-59 1.3566210397728824 1101 60-61 1.3678960868798313 1101 62-63 1.4543946868064594 1101 64-65 1.4052186916482228 1101 66-67 1.1727277222354608 1101 68-69 1.3797610604627764 1101 70-71 1.6741260522342323 1101 72-73 1.6387450759751765 1101 74-75 1.49976877160994 1101 76-77 1.5238636587152463 1101 78-79 1.6006542760148292 1101 80-81 1.8175173611510296 1101 82-83 1.629445040261185 1101 84-85 1.5735399696393912 1101 86-87 1.5197116940370528 1101 88-89 1.8537619408394086 1101 90-91 2.0659994152229153 1101 92-93 2.023501804735176 1101 94-95 2.117898718174409 1101 96-97 2.2337856245231364 1101 98-99 2.432565820252851 1101 100-101 1.9960465723815783 1102 1 0.5519544117509625 1102 2 0.5546032531238865 1102 3 0.5478792346103845 1102 4 0.5184569584769889 1102 5 0.4820843422109746 1102 6 0.377069014719531 1102 7 0.413151114158552 1102 8 0.5018009733179412 1102 9 0.5373408287366885 1102 10-11 0.7162458670356315 1102 12-13 0.6753809597640341 1102 14-15 0.7826596153356533 1102 16-17 0.6447210296789692 1102 18-19 0.8623259401735908 1102 20-21 0.6203906880209189 1102 22-23 0.7570952124866039 1102 24-25 0.5409628024528672 1102 26-27 0.45017139091536507 1102 28-29 0.43327807315706224 1102 30-31 0.37092836154486264 1102 32-33 0.39577626266584076 1102 34-35 0.45252372263927043 1102 36-37 0.5230623951562734 1102 38-39 0.5066106369258137 1102 40-41 0.5891057310752501 1102 42-43 0.5360553648910908 1102 44-45 0.6388865297778992 1102 46-47 0.5570413766864917 1102 48-49 0.4443850790705355 1102 50-51 0.5339829596757397 1102 52-53 0.5892971909550724 1102 54-55 0.50903437634868 1102 56-57 0.5396830665595971 1102 58-59 0.6874585869797869 1102 60-61 0.6711854523858918 1102 62-63 0.4739236542079226 1102 64-65 0.5741027636614078 1102 66-67 0.4304682879523405 1102 68-69 0.6202687980826624 1102 70-71 0.7759289259701383 1102 72-73 0.8032667778926985 1102 74-75 0.7558228505913469 1102 76-77 0.7783020847430855 1102 78-79 0.8976984854000243 1102 80-81 0.9065855527926949 1102 82-83 1.0154258163209207 1102 84-85 0.8811426973693308 1102 86-87 0.9213480749101635 1102 88-89 0.9906387843254016 1102 90-91 1.2175431364040143 1102 92-93 1.3532036012084276 1102 94-95 1.3358519887669544 1102 96-97 1.5184458392900915 1102 98-99 1.6916052284734278 1102 100-101 1.5276761992736692 1103 1 0.667601846344013 1103 2 0.6124828138629361 1103 3 0.5957207453106506 1103 4 0.5918686460250271 1103 5 0.66900381902677 1103 6 0.5966237563377845 1103 7 0.5407041667039181 1103 8 0.539622028488715 1103 9 0.6018150155516153 1103 10-11 0.6396955995225895 1103 12-13 0.7174763332240701 1103 14-15 0.7805278747898967 1103 16-17 0.8163561052767037 1103 18-19 0.8547048402260629 1103 20-21 0.8127769573678592 1103 22-23 0.8546056404207718 1103 24-25 0.912447151754364 1103 26-27 0.9072447406396407 1103 28-29 0.8045205617040594 1103 30-31 1.035492994337254 1103 32-33 1.1545192038252168 1103 34-35 1.1153355343178362 1103 36-37 1.0010971802661146 1103 38-39 0.8328811772404521 1103 40-41 1.1478253658846356 1103 42-43 1.0630954482431676 1103 44-45 0.9809243003444763 1103 46-47 0.9213975272934576 1103 48-49 1.0714808018708268 1103 50-51 1.2099878483993933 1103 52-53 1.1606454760514069 1103 54-55 1.104409876325981 1103 56-57 1.2158200415993647 1103 58-59 1.0642354411733166 1103 60-61 1.23557702150503 1103 62-63 1.256451694500882 1103 64-65 1.03745875566279 1103 66-67 1.3052697122406265 1103 68-69 1.310444406584942 1103 70-71 1.3545479507855411 1103 72-73 1.3657371030953307 1103 74-75 1.3173276452226226 1103 76-77 1.3455294297724372 1103 78-79 1.3816155739524234 1103 80-81 1.4112215784939401 1103 82-83 1.6910586704206203 1103 84-85 1.6732068342005526 1103 86-87 1.7965168164539627 1103 88-89 1.7256221256400117 1103 90-91 1.8960729647070984 1103 92-93 1.9538535015827065 1103 94-95 1.7125795018366148 1103 96-97 1.8579464306186004 1103 98-99 2.075485929540676 1103 100-101 1.7819321580749783 1104 1 0.37589509485573913 1104 2 0.5347125925758291 1104 3 0.4520354920383376 1104 4 0.4642014999934858 1104 5 0.44462563555990187 1104 6 0.48477565124589006 1104 7 0.39433541468196864 1104 8 0.41611260530871164 1104 9 0.4787103369031911 1104 10-11 0.5161629165197397 1104 12-13 0.4766885218435206 1104 14-15 0.5479647131542009 1104 16-17 0.5718744687297388 1104 18-19 0.6086766025649908 1104 20-21 0.6785334211191412 1104 22-23 0.6820235403303272 1104 24-25 0.6894585360798473 1104 26-27 0.6451504592500967 1104 28-29 0.6013193252732734 1104 30-31 0.6583143045855095 1104 32-33 0.5852320878779409 1104 34-35 0.5644263940339442 1104 36-37 0.4882673217377942 1104 38-39 0.6196947468085767 1104 40-41 0.5903902513447719 1104 42-43 0.5120370672685226 1104 44-45 0.5691314520906161 1104 46-47 0.6332328808073484 1104 48-49 0.49761457064693815 1104 50-51 0.40525831173033566 1104 52-53 0.31762212413382684 1104 54-55 0.5065511968699568 1104 56-57 0.5235610759412523 1104 58-59 0.27877861589119135 1104 60-61 0.3738903183310711 1104 62-63 0.4524246753239254 1104 64-65 0.3795534857189651 1104 66-67 0.46311004359273156 1104 68-69 0.5883852392629869 1104 70-71 0.6139821113184603 1104 72-73 0.6720538570158503 1104 74-75 0.4148200710214276 1104 76-77 0.6163673973598129 1104 78-79 0.559212114024259 1104 80-81 0.5324551992100588 1104 82-83 0.5130180684516823 1104 84-85 0.5051071499201782 1104 86-87 0.6095518809320808 1104 88-89 0.5609813068942522 1104 90-91 0.6057731373256843 1104 92-93 0.7869617931782358 1104 94-95 0.5143360317071881 1104 96-97 0.7254199888047559 1104 98-99 0.9029883676896198 1104 100-101 0.4501031567360645 1105 1 0.33879082052758847 1105 2 0.39885339817378096 1105 3 0.34123855163259265 1105 4 0.3468364282786638 1105 5 0.3214765205038006 1105 6 0.3620464717491032 1105 7 0.37370814430772725 1105 8 0.296200698060467 1105 9 0.46853995284735106 1105 10-11 0.5035492304583826 1105 12-13 0.43982523072688195 1105 14-15 0.6356219100731835 1105 16-17 0.4932106313844855 1105 18-19 0.4799951377440763 1105 20-21 0.5274081634009846 1105 22-23 0.5610747358748185 1105 24-25 0.4867907626754686 1105 26-27 0.6333284476650505 1105 28-29 0.6579762633584281 1105 30-31 0.5174417668537927 1105 32-33 0.49734684943844343 1105 34-35 0.5766288467075213 1105 36-37 0.5903188028920141 1105 38-39 0.6665238093838752 1105 40-41 0.7297051003732449 1105 42-43 0.7087297958232455 1105 44-45 0.6549328551484237 1105 46-47 0.724042718277552 1105 48-49 0.6512197241175457 1105 50-51 0.4931567149934324 1105 52-53 0.42158071964797017 1105 54-55 0.5074990641685346 1105 56-57 0.5773449586270587 1105 58-59 0.6760791576571492 1105 60-61 0.6544784512466961 1105 62-63 0.6448919417710215 1105 64-65 0.5913844226081508 1105 66-67 0.6932423165999388 1105 68-69 0.4478237483801806 1105 70-71 0.5930817266378519 1105 72-73 0.5415476137656121 1105 74-75 0.3937951783482099 1105 76-77 0.3415264909059239 1105 78-79 0.40626341667239885 1105 80-81 0.42759224725496736 1105 82-83 0.45542575849372113 1105 84-85 0.2880854792817509 1105 86-87 0.4368476922868503 1105 88-89 0.7765044189692958 1105 90-91 0.3748210751341645 1105 92-93 0.6745556204379 1105 94-95 0.6766797273186143 1105 96-97 0.9474309044517106 1105 98-99 0.9139274435636295 1105 100-101 0.5813049043106115 1106 1 0.2979806269947147 1106 2 0.41550203382710293 1106 3 0.16265138170030724 1106 4 0.4598307785611482 1106 5 0.5484809681537612 1106 6 0.08489777599240256 1106 7 0.2992641005524703 1106 8 0.38961937819028947 1106 9 0.4433165686264928 1106 10-11 0.15616773428497055 1106 12-13 0.31230189058182845 1106 14-15 0.5640207560883326 1106 16-17 0.37820983000611363 1106 18-19 0.015039413899060605 1106 20-21 0.3638778913330327 1106 22-23 0.4233448404546962 1106 24-25 0.48672865585173497 1106 26-27 0.2788367218967309 1106 28-29 0.30217027706200383 1106 30-31 0.3762789668377593 1106 32-33 0.5618838530847228 1106 34-35 0.48140005318266077 1106 36-37 0.3426167553702726 1106 38-39 0.490796558512379 1106 40-41 0.5964869651807234 1106 42-43 0.24943581016791683 1106 44-45 0.47732837544333506 1106 46-47 0.2032058789138489 1106 48-49 0.6611838624354434 1106 50-51 0.28470718179632826 1106 52-53 0.5887312185060054 1106 54-55 0.4077354707879195 1106 56-57 0.1013282098191084 1106 58-59 -0.3576167993889712 1106 60-61 0.3477959573572065 1106 62-63 0.5468523136105858 1106 64-65 0.6935401304255961 1106 66-67 0.08163435089893412 1106 68-69 -0.07771618029714134 1106 70-71 -0.11749246096482224 1106 72-73 0.5012002162418696 1106 74-75 0.6612812543990181 1106 76-77 0.6127770418535547 1106 78-79 0.7896979240250417 1106 80-81 0.7674047247162044 1106 82-83 0.98368235984805 1106 84-85 0.9196708060437828 1106 86-87 0.9275316687263278 1106 88-89 0.5920301231805389 1106 90-91 0.536110492932778 1106 92-93 0.5620461040697506 1106 94-95 0.6329645371915973 1106 96-97 0.7505552783748577 1106 98-99 0.6706740477227839 1106 100-101 0.5355932803173449 1107 1 0.38381224143529735 1107 2 0.32706146984191165 1107 3 0.27602295451072223 1107 4 0.3752800924168369 1107 5 0.34904796729667 1107 6 0.30695234730525556 1107 7 0.2465444825704992 1107 8 0.2671136563966172 1107 9 0.2570356874231763 1107 10-11 0.3776873296623293 1107 12-13 0.3597800629985386 1107 14-15 0.4882321846059412 1107 16-17 0.5053400796929495 1107 18-19 0.47987133915313507 1107 20-21 0.4497179825049784 1107 22-23 0.49887063326454495 1107 24-25 0.3823066276524685 1107 26-27 0.41409427325159953 1107 28-29 0.5260351014523863 1107 30-31 0.5027847639801664 1107 32-33 0.46373178052390074 1107 34-35 0.5251164405881212 1107 36-37 0.5278342777158826 1107 38-39 0.5312635322250969 1107 40-41 0.5813409299363528 1107 42-43 0.5228518387250816 1107 44-45 0.6540484455176454 1107 46-47 0.6401776137240134 1107 48-49 0.5405015797458717 1107 50-51 0.4573273394635322 1107 52-53 0.35679560399007215 1107 54-55 0.3317575856382078 1107 56-57 0.4972791578002358 1107 58-59 0.3243767394580104 1107 60-61 0.5079543780479696 1107 62-63 0.5688880477426181 1107 64-65 0.5059971581068297 1107 66-67 0.39388982074531853 1107 68-69 0.4153997719694438 1107 70-71 0.5164841620937715 1107 72-73 0.5419996661961868 1107 74-75 0.772061772986607 1107 76-77 0.7477134192753105 1107 78-79 0.8102210325366208 1107 80-81 0.6942806724618613 1107 82-83 0.744517977189556 1107 84-85 0.7537585684692587 1107 86-87 0.8053960975359153 1107 88-89 0.7388537035634961 1107 90-91 0.9078415356649252 1107 92-93 0.9387718252983106 1107 94-95 1.0433749051759236 1107 96-97 1.248174258718258 1107 98-99 1.2210164134843247 1107 100-101 0.755143448439183 1108 1 0.3369054953580175 1108 2 0.3856511155911164 1108 3 0.3913383875062557 1108 4 0.31002523531298465 1108 5 0.45571488501446566 1108 6 0.45570779075079315 1108 7 0.3225457009530146 1108 8 0.25582223957975714 1108 9 0.15269354239132582 1108 10-11 0.33935280285545844 1108 12-13 0.45159985614418474 1108 14-15 0.5795025092052484 1108 16-17 0.4826582966101469 1108 18-19 0.5427244410957712 1108 20-21 0.45748978016639796 1108 22-23 0.46518692669859263 1108 24-25 0.43152912426573664 1108 26-27 0.47228625793434276 1108 28-29 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-1.0328530697143385 2316 80-81 -1.1245990292783574 2316 82-83 -0.9054925335313762 2316 84-85 -0.9275986381036709 2316 86-87 -1.1483144650156696 2316 88-89 -1.3691047473898088 2316 90-91 -1.5700681544412056 2316 92-93 -1.6338236109355364 2316 94-95 -1.8426151936137316 2316 96-97 -1.8494714510167825 2316 98-99 -1.6381632831395656 2316 100-101 -1.314793998868181 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7161.0 3 1734.0 4 1601.0 5 1674.0 6 1984.0 7 2475.0 8 3080.0 9 4198.0 10 5451.0 11 7596.0 12 10208.0 13 13024.0 14 15506.0 15 19772.0 16 23747.0 17 29165.0 18 25782.0 19 20869.0 20 21586.0 21 22919.0 22 25571.0 23 28903.0 24 32953.0 25 37278.0 26 42909.0 27 49750.0 28 57636.0 29 66600.0 30 78850.0 31 92913.0 32 110395.0 33 133387.0 34 164743.0 35 206913.0 36 267685.0 37 315606.0 38 179268.0 39 10402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.884822424921566 21.318180010433895 15.051198611309314 22.74579895333522 2 14.630765176632416 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28.89503966127318 30.96806478813578 20.15155559198382 46-47 20.439209938679593 28.709595156392965 30.17541868495303 20.675776219974416 48-49 20.156703340227143 28.780594773036803 30.395758720479467 20.666943166256587 50-51 19.972416150890098 29.284814217685422 29.894825482880826 20.84794414854365 52-53 19.966185368633194 29.420714264029684 30.02968121302984 20.58341915430728 54-55 20.31574970003218 28.990039060037 29.943327216434078 20.75088402349674 56-57 20.434195955643506 29.17026099116018 29.435731142815374 20.95981191038094 58-59 20.349400392701213 29.00204477391657 29.646769742950713 21.001785090431504 60-61 20.349579751865495 28.836838261858105 29.86144924246511 20.95213274381129 62-63 20.258200466139503 28.638927295361093 30.06483033264688 21.038041905852523 64-65 20.203522280054823 28.65952577165031 29.724401680131834 21.412550268163034 66-67 20.197841499825568 28.84765102332374 29.44131096030327 21.513196516547428 68-69 20.40103647560929 28.36441234467111 29.835687417040386 21.39886376267922 70-71 20.38625997985811 28.238280615018972 29.806634118269145 21.568825286853773 72-73 20.382739360987816 28.064478083220358 29.758055381367264 21.794727174424565 74-75 20.16502876477854 28.305415550800028 29.65968276418393 21.869872920237498 76-77 19.91561106546868 28.240798042445746 30.000504427990336 21.843086464095236 78-79 19.815662822222276 27.91134348503282 29.845926437930398 22.427067254814506 80-81 19.956317897168883 28.09945956649961 29.582726864493242 22.36149567183826 82-83 19.951666600892644 28.08969584145487 29.72123411995174 22.23740343770075 84-85 20.02899327755739 27.56133750173969 29.758390910458036 22.651278310244884 86-87 19.733219125219716 27.958141543177977 29.595181588331904 22.713457743270403 88-89 20.24088973223107 27.39211534230334 29.59965226149151 22.767342663974073 90-91 20.121482909983797 27.33068821628854 29.779617182142143 22.76821169158552 92-93 20.03401478115538 27.30148624321176 29.907293139028994 22.757205836603866 94-95 19.959549660044768 27.251565471133876 29.810975594089552 22.977909274731807 96-97 20.313855875203245 26.89983992570198 29.47158663691445 23.314717562180327 98-99 20.23975011841069 26.59779695392586 29.399973094849063 23.762479832814382 100-101 20.372409056149614 26.31279826494636 29.88983849478439 23.42495418411964 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 254.0 1 161.0 2 104.0 3 130.5 4 114.0 5 133.0 6 222.5 7 427.5 8 733.0 9 962.5 10 1016.5 11 1010.5 12 1143.5 13 1380.0 14 1561.5 15 1740.5 16 2084.5 17 2469.5 18 2806.5 19 3302.0 20 3901.5 21 4609.0 22 5404.5 23 6345.0 24 7789.5 25 10086.5 26 13693.0 27 18229.0 28 21871.5 29 26221.5 30 33120.0 31 40532.0 32 49702.5 33 59900.5 34 70740.5 35 81569.5 36 91016.0 37 97634.5 38 96313.0 39 92569.0 40 91107.5 41 90200.0 42 88482.5 43 88098.0 44 88468.5 45 85779.5 46 80866.5 47 76361.0 48 74426.0 49 71806.5 50 66835.5 51 60635.5 52 53959.5 53 47558.5 54 41576.5 55 35791.0 56 30319.5 57 25087.0 58 20418.0 59 16502.0 60 13159.0 61 10396.5 62 7872.5 63 5544.0 64 3845.0 65 2775.5 66 2076.5 67 1617.5 68 1275.5 69 1049.0 70 846.5 71 700.0 72 580.0 73 504.5 74 423.5 75 334.5 76 266.5 77 208.5 78 167.5 79 136.0 80 113.5 81 92.5 82 62.5 83 35.0 84 17.0 85 11.5 86 9.5 87 4.5 88 3.5 89 2.5 90 2.0 91 1.0 92 0.5 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1881572544452093 2 0.04810175529376162 3 0.04623372596196505 4 0.051464208090995445 5 0.05758200415262921 6 0.06388660314744263 7 0.03549255730413479 8 0.034371739705056845 9 0.03992912696715164 10-11 0.04135349933264652 12-13 0.03747733846916865 14-15 0.03308746953944671 16-17 0.031219440207650138 18-19 0.039298667067670295 20-21 0.04936267509272431 22-23 0.04593017119554811 24-25 0.0662916909121307 26-27 0.05179111322405985 28-29 0.042941324264673605 30-31 0.051931215423944584 32-33 0.04812510566040908 34-35 0.04777485016069722 36-37 0.04534641202936168 38-39 0.02549860037902315 40-41 0.023373717014104553 42-43 0.02068842484964699 44-45 0.004646722962843962 46-47 0.011441679657253978 48-49 0.011301577457369235 50-51 0.00868633639285404 52-53 0.009760453258637067 54-55 0.01078786939112518 56-57 0.008733037126148955 58-59 0.010554365724650608 60-61 0.011091424157542122 62-63 0.00417971562989482 64-65 0.0071218618274744155 66-67 0.003969562330067707 68-69 0.008966540792623526 70-71 0.02250975344814864 72-73 0.019217351750857193 74-75 0.018423439284843652 76-77 0.01190868699020312 78-79 0.0050903799291456475 80-81 0.006654854494525273 82-83 0.0058142412952168175 84-85 0.005043679195850734 86-87 0.008102577226667613 88-89 0.02825394364342309 90-91 0.03483874703800599 92-93 0.02148233731566053 94-95 0.0300986226085722 96-97 0.03388138200546025 98-99 0.020548322649762245 100-101 0.12004423493457694 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2141294.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.54060352501876 #Duplication Level Percentage of deduplicated Percentage of total 1 76.55051200984417 43.282121491857865 2 13.750244669604331 15.548942644722022 3 4.664751510250789 7.912435970514671 4 2.0170846177539374 4.561887265953576 5 0.978951968179649 2.767526755144116 6 0.5393696941709621 1.82977728189186 7 0.34736785066749304 1.3748271545350053 8 0.20645160541961644 0.9338318695307317 9 0.14350960611371316 0.7302707767176358 >10 0.6415803968580294 6.844764865561899 >50 0.08533525717139381 3.388937263681382 >100 0.0691245570198953 7.544565116252114 >500 0.005053502517535344 1.9172354319421139 >1k 4.142215178307579E-4 0.31706448996643066 >5k 2.4853291069845473E-4 1.0458116217285416 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9682 0.4521564997613593 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7513 0.35086260924469037 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5132 0.23966816326949966 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.6700733294914194E-5 0.0 8 0.0 0.0 0.0 4.6700733294914194E-5 0.0 9 0.0 0.0 0.0 9.340146658982839E-5 0.0 10-11 0.0 0.0 0.0 9.340146658982839E-5 0.0 12-13 0.0 0.0 0.0 9.340146658982839E-5 0.0 14-15 0.0 0.0 0.0 2.1015329982711388E-4 0.0 16-17 0.0 4.6700733294914194E-5 0.0 2.5685403312202806E-4 0.0 18-19 0.0 4.6700733294914194E-5 0.0 2.802043997694852E-4 0.0 20-21 0.0 4.6700733294914194E-5 0.0 3.969562330067707E-4 0.0 22-23 0.0 4.6700733294914194E-5 0.0 0.0011208175990779408 0.0 24-25 0.0 4.6700733294914194E-5 0.0 0.0020548322649762245 0.0 26-27 0.0 4.6700733294914194E-5 0.0 0.003572606097060936 0.0 28-29 0.0 4.6700733294914194E-5 0.0 0.010390913158118409 0.0 30-31 0.0 4.6700733294914194E-5 0.0 0.03472199520476871 0.0 32-33 0.0 4.6700733294914194E-5 0.0 0.0862095536624116 0.0 34-35 0.0 4.6700733294914194E-5 0.0 0.16574090246365047 0.0 36-37 0.0 4.6700733294914194E-5 0.0 0.2941445686580171 0.0 38-39 0.0 4.6700733294914194E-5 0.0 0.474362698443091 0.0 40-41 0.0 4.6700733294914194E-5 0.0 0.6646448362532189 0.0 42-43 0.0 4.6700733294914194E-5 0.0 0.8575889158611568 4.6700733294914194E-5 44-45 0.0 4.6700733294914194E-5 0.0 1.1021606561266224 4.6700733294914194E-5 46-47 0.0 4.6700733294914194E-5 0.0 1.3815244426967992 4.6700733294914194E-5 48-49 0.0 4.6700733294914194E-5 0.0 1.6631999155650743 4.6700733294914194E-5 50-51 0.0 4.6700733294914194E-5 0.0 1.9540053817925047 4.6700733294914194E-5 52-53 0.0 4.6700733294914194E-5 0.0 2.248873811816593 4.6700733294914194E-5 54-55 0.0 4.6700733294914194E-5 0.0 2.57570889378105 4.6700733294914194E-5 56-57 0.0 4.6700733294914194E-5 0.0 2.957837643966686 4.6700733294914194E-5 58-59 0.0 4.6700733294914194E-5 0.0 3.3690843013617 4.6700733294914194E-5 60-61 0.0 4.6700733294914194E-5 0.0 3.792870105646399 4.6700733294914194E-5 62-63 0.0 4.6700733294914194E-5 0.0 4.21240614320126 4.6700733294914194E-5 64-65 0.0 4.6700733294914194E-5 0.0 4.654101678704559 4.6700733294914194E-5 66-67 0.0 4.6700733294914194E-5 0.0 5.158236094623158 4.6700733294914194E-5 68-69 0.0 4.6700733294914194E-5 0.0 5.708347382470599 4.6700733294914194E-5 70-71 0.0 4.6700733294914194E-5 0.0 6.2949319430213695 4.6700733294914194E-5 72-73 0.0 4.6700733294914194E-5 0.0 6.871242342247258 4.6700733294914194E-5 74-75 0.0 4.6700733294914194E-5 0.0 7.46634978662435 4.6700733294914194E-5 76-77 0.0 4.6700733294914194E-5 0.0 8.12704841091415 4.6700733294914194E-5 78-79 0.0 4.6700733294914194E-5 0.0 8.837249812496555 4.6700733294914194E-5 80-81 0.0 4.6700733294914194E-5 0.0 9.578623953553318 4.6700733294914194E-5 82-83 0.0 4.6700733294914194E-5 0.0 10.31294628388255 4.6700733294914194E-5 84-85 0.0 4.6700733294914194E-5 0.0 11.063216914631994 4.6700733294914194E-5 86-87 0.0 4.6700733294914194E-5 0.0 11.859464417310281 4.6700733294914194E-5 88-89 0.0 4.6700733294914194E-5 0.0 12.693656265790686 4.6700733294914194E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5170 0.0 43.567406 1 GTATCAA 9095 0.0 39.79257 1 TCAACGC 11515 0.0 30.831465 4 TATCAAC 11600 0.0 30.809683 2 CAACGCA 11740 0.0 30.239866 5 AACGCAG 12050 0.0 29.495146 6 ATCAACG 12295 0.0 28.91416 3 GTACATG 9525 0.0 26.397331 1 ACGCAGA 13535 0.0 26.185799 7 CGCAGAG 13575 0.0 26.038643 8 TACATGG 10010 0.0 25.115911 2 GCAGAGT 14210 0.0 24.205809 9 ACATGGG 10165 0.0 24.032183 3 CATGGGT 2775 0.0 22.264753 4 GAGTACT 8185 0.0 22.19815 12-13 AGTACTT 8600 0.0 21.927855 12-13 ACCGCAT 130 2.93846E-4 21.927702 8 GTATAGG 710 0.0 21.46262 1 TAGCCCT 2050 0.0 21.329048 4 CTACACG 380 0.0 21.261936 4 >>END_MODULE