FastQCFastQC Report
Thu 26 May 2016
SRR938526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938526_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141294
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91420.4269381037821056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74480.3478270615805209No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51510.24055547720210302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56550.045.666151
GTATCAA93750.036.2712021
TATCAAC119200.028.4073622
TCAACGC119150.028.3248844
CAACGCA120400.028.0308155
AACGCAG122950.027.4662886
ATCAACG126000.026.7853
CGCAGAG139250.024.148888
ACGCAGA139650.024.1477327
ACGCCGA1601.8620995E-623.7481026
CGCCGAA1601.8620995E-623.7481027
GTACATG93100.023.5493241
GAGTACT83650.022.62670512-13
TACATGG96500.022.325342
GCAGAGT149200.022.092749
CTACACG3450.022.0447274
CTCCGAC3250.021.92132694-95
AGTACTT87750.021.59656512-13
CATTCCG4700.021.2217089
ACATGGG97000.021.1207523
Produced by FastQC (version 0.11.5)