##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938526_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2141294 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.798022597550826 31.0 31.0 34.0 30.0 34.0 2 32.032442532412645 31.0 31.0 34.0 30.0 34.0 3 32.09377040238286 33.0 31.0 34.0 30.0 34.0 4 35.76527230730577 37.0 35.0 37.0 35.0 37.0 5 35.640124616236726 37.0 35.0 37.0 33.0 37.0 6 35.66470087713317 37.0 35.0 37.0 33.0 37.0 7 35.6048646285844 37.0 35.0 37.0 33.0 37.0 8 35.60070779631382 37.0 35.0 37.0 33.0 37.0 9 37.17225005067029 39.0 37.0 39.0 33.0 39.0 10-11 37.09110262299339 39.0 37.0 39.0 33.0 39.0 12-13 37.00998625130411 39.0 37.0 39.0 33.0 39.0 14-15 38.06205943695728 40.0 37.0 41.0 33.0 41.0 16-17 38.00095713152888 40.0 37.0 41.0 33.0 41.0 18-19 38.14514914813192 40.0 37.5 41.0 33.0 41.0 20-21 38.21734381173253 40.0 37.5 41.0 33.0 41.0 22-23 38.1160872818025 40.0 37.0 41.0 33.0 41.0 24-25 38.03933486013598 40.0 37.0 41.0 33.0 41.0 26-27 37.880210984572884 40.0 37.0 41.0 33.0 41.0 28-29 37.76710087451793 40.0 37.0 41.0 32.5 41.0 30-31 37.54569036293008 39.0 36.5 41.0 32.0 41.0 32-33 37.33352683003828 39.0 36.0 41.0 31.5 41.0 34-35 37.18673101405038 39.0 36.0 41.0 31.0 41.0 36-37 37.176521766744784 39.0 36.0 41.0 31.0 41.0 38-39 37.05872196905236 39.0 36.0 41.0 31.0 41.0 40-41 36.90330776623854 39.0 36.0 41.0 31.0 41.0 42-43 36.70347275992928 39.0 35.0 41.0 30.0 41.0 44-45 36.408352612952726 39.0 35.0 41.0 30.0 41.0 46-47 36.00539626973223 39.0 35.0 40.0 29.5 41.0 48-49 35.65068435254571 39.0 35.0 40.0 27.5 41.0 50-51 35.337052268394714 38.5 35.0 40.0 27.0 41.0 52-53 34.933035818528424 38.0 34.0 40.0 26.0 41.0 54-55 34.687969050490025 38.0 34.0 40.0 24.5 41.0 56-57 34.41264931391953 38.0 34.0 40.0 24.0 41.0 58-59 34.04265855132458 38.0 33.0 40.0 23.0 41.0 60-61 33.75426260943149 37.5 33.0 40.0 22.5 41.0 62-63 33.348222850295194 37.0 32.0 39.5 21.5 41.0 64-65 32.914732867135484 36.5 31.5 39.0 20.0 40.5 66-67 32.48313169513388 36.0 31.0 39.0 18.5 40.0 68-69 31.983747911309706 35.5 31.0 38.5 17.0 40.0 70-71 31.470333592678074 35.0 30.0 38.0 13.0 40.0 72-73 31.060401327421644 35.0 30.0 37.5 12.0 39.5 74-75 30.577251419001783 34.0 29.0 37.0 8.0 39.0 76-77 29.20231131269223 32.5 28.5 35.5 7.5 38.0 78-79 29.921669794059106 34.0 29.0 36.0 7.0 38.5 80-81 29.65204100884792 34.0 29.0 36.0 4.0 37.5 82-83 29.202576105850014 34.0 29.0 35.0 2.0 37.0 84-85 28.7087966435249 34.0 28.5 35.0 2.0 36.5 86-87 28.174143064894405 33.0 27.5 35.0 2.0 36.0 88-89 27.59386964144111 33.0 26.0 35.0 2.0 35.5 90-91 27.108922221796725 32.5 26.0 35.0 2.0 35.0 92-93 26.465713489133208 32.0 25.0 34.0 2.0 35.0 94-95 25.78660893833355 32.0 23.5 34.0 2.0 35.0 96-97 24.880906825498975 31.0 19.0 34.0 2.0 35.0 98-99 23.756884622102334 31.0 4.0 34.0 2.0 35.0 100-101 20.122684460891406 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4239558982437224 1101 2 0.49007362706907287 1101 3 0.444381063025574 1101 4 0.33200610058352 1101 5 0.26926523232751975 1101 6 0.26060185723770246 1101 7 0.355218192116979 1101 8 0.3556214825066917 1101 9 0.39035716573360446 1101 10-11 0.39494301338032756 1101 12-13 0.43429977992182245 1101 14-15 0.6516499938226801 1101 16-17 0.7775732956556993 1101 18-19 0.6598005103379734 1101 20-21 0.48422157111240693 1101 22-23 0.5872565114674657 1101 24-25 0.6299371238933915 1101 26-27 0.6930255330661055 1101 28-29 0.6220360684756585 1101 30-31 0.6612391351033722 1101 32-33 0.6941838319694753 1101 34-35 0.4918961697391282 1101 36-37 0.8008099626936556 1101 38-39 0.8348612157331061 1101 40-41 0.9680284939176431 1101 42-43 0.9885601091095211 1101 44-45 0.9410405411224119 1101 46-47 1.0357480501856742 1101 48-49 1.0840602764569738 1101 50-51 1.0155132245645646 1101 52-53 0.8948837331849617 1101 54-55 0.9446107132908708 1101 56-57 0.9932591871239822 1101 58-59 1.1833687966635367 1101 60-61 1.2490596866846815 1101 62-63 1.1995973322872402 1101 64-65 1.3439975973985199 1101 66-67 1.3414407016409982 1101 68-69 1.1182721147338555 1101 70-71 1.0594267844312242 1101 72-73 0.9033110407593519 1101 74-75 0.9915724388342291 1101 76-77 0.9790390084501794 1101 78-79 1.0355826431214084 1101 80-81 1.1556792481011833 1101 82-83 1.150984517421353 1101 84-85 1.0461057000089475 1101 86-87 1.1447803047760914 1101 88-89 1.3399829143521202 1101 90-91 1.3459672173116992 1101 92-93 1.4053602548970439 1101 94-95 1.4827544043420389 1101 96-97 1.5750352698198036 1101 98-99 1.3564053637328435 1101 100-101 1.0316288044822137 1102 1 0.30678825329423987 1102 2 0.3662500168439422 1102 3 0.3581405988058748 1102 4 0.22938442046513075 1102 5 0.21281191326271198 1102 6 0.21303797694974236 1102 7 0.2521002738214051 1102 8 0.2153400648455417 1102 9 0.17317757678913637 1102 10-11 0.24258503846057522 1102 12-13 0.3569488883802592 1102 14-15 0.393302721365167 1102 16-17 0.43769446561017844 1102 18-19 0.45298139973176177 1102 20-21 0.35279089475556447 1102 22-23 0.41848384532676164 1102 24-25 0.43556828904349487 1102 26-27 0.36238187492573815 1102 28-29 0.366464301937377 1102 30-31 0.271927832281321 1102 32-33 0.22147331637987833 1102 34-35 0.33649100318466907 1102 36-37 0.27393115353673636 1102 38-39 0.3658925716657606 1102 40-41 0.40945458013829494 1102 42-43 0.36807305120471057 1102 44-45 0.32986430935106625 1102 46-47 0.15606465174953854 1102 48-49 0.2934746618473767 1102 50-51 0.40316187508059187 1102 52-53 0.3952831607301803 1102 54-55 0.4070868855564882 1102 56-57 0.41043052774904254 1102 58-59 0.4944509493892113 1102 60-61 0.5163255884000719 1102 62-63 0.6745691728461551 1102 64-65 0.6329865730375914 1102 66-67 0.5402178347287148 1102 68-69 0.484119626874687 1102 70-71 0.3844274405475403 1102 72-73 0.3085523121360261 1102 74-75 0.39364185647609773 1102 76-77 0.407499031149122 1102 78-79 0.44654873020973795 1102 80-81 0.6101811969005446 1102 82-83 0.3072692815435225 1102 84-85 0.2633226474130588 1102 86-87 0.2449436911289773 1102 88-89 0.288908945673235 1102 90-91 0.3436195260109436 1102 92-93 0.3459214726917743 1102 94-95 0.3809878178554662 1102 96-97 0.3723657601609709 1102 98-99 0.38707625739539964 1102 100-101 0.35687168803040237 1103 1 0.2723843578188827 1103 2 0.2682299856226109 1103 3 0.3200681206472922 1103 4 0.18397554191875543 1103 5 0.15378870941986378 1103 6 0.17930241783448508 1103 7 0.19795151403031497 1103 8 0.2231948657751417 1103 9 0.31143089590406703 1103 10-11 0.32351952619771396 1103 12-13 0.344187386798545 1103 14-15 0.516920213673302 1103 16-17 0.4072782764176921 1103 18-19 0.3698565230845716 1103 20-21 0.33434738664501396 1103 22-23 0.3310421940744135 1103 24-25 0.39804544805552666 1103 26-27 0.48824853200466833 1103 28-29 0.3516316206208572 1103 30-31 0.36374465151023827 1103 32-33 0.4020849066226262 1103 34-35 0.41810185955787915 1103 36-37 0.4284558238436773 1103 38-39 0.4249700889938026 1103 40-41 0.4084286162524222 1103 42-43 0.5268221886912698 1103 44-45 0.6458715134101425 1103 46-47 0.5979080412374032 1103 48-49 0.54082541323492 1103 50-51 0.6207864480312537 1103 52-53 0.6582054353279361 1103 54-55 0.7055072301130778 1103 56-57 0.7406452980590998 1103 58-59 0.7318564681492958 1103 60-61 0.6046964337371961 1103 62-63 0.5922708556462197 1103 64-65 0.8082240606640525 1103 66-67 0.7948005669163081 1103 68-69 0.7121370724681242 1103 70-71 0.5767316021844664 1103 72-73 0.6175412194353989 1103 74-75 0.8313111398797837 1103 76-77 0.6499205150139389 1103 78-79 0.5993555134285558 1103 80-81 0.6352135937469932 1103 82-83 0.7655720794784884 1103 84-85 0.5478718888347167 1103 86-87 0.6636972195930717 1103 88-89 0.805485523352484 1103 90-91 0.775100259513291 1103 92-93 1.0041103908028468 1103 94-95 0.7924984008253055 1103 96-97 1.006315819293821 1103 98-99 0.9291839980105152 1103 100-101 0.692529154043676 1104 1 0.2708624333696257 1104 2 0.2240941765940221 1104 3 0.23783130495245786 1104 4 0.1765886000069159 1104 5 0.04797517054998224 1104 6 0.12323797986028495 1104 7 0.1893305027357215 1104 8 0.17635477764756757 1104 9 0.2364343789739891 1104 10-11 0.3588547392080983 1104 12-13 0.2243903258011315 1104 14-15 0.41425548808017965 1104 16-17 0.36860687277593485 1104 18-19 0.3014814991753596 1104 20-21 0.2604720640182805 1104 22-23 0.35339145979605036 1104 24-25 0.29695967480219565 1104 26-27 0.3126666231879014 1104 28-29 0.38002064765183974 1104 30-31 0.3182804350356676 1104 32-33 0.33316109257841475 1104 34-35 0.3115845044240757 1104 36-37 0.3780752836962762 1104 38-39 0.37227903375349314 1104 40-41 0.22237400171609778 1104 42-43 0.2702154544412352 1104 44-45 0.32696104129477277 1104 46-47 0.2085919744852518 1104 48-49 0.33758663049110993 1104 50-51 0.4408039460299662 1104 52-53 0.30901704965410204 1104 54-55 0.34218053232311263 1104 56-57 0.3007694606464426 1104 58-59 0.33754107439708747 1104 60-61 0.16196365249995637 1104 62-63 0.13780851176450426 1104 64-65 0.19726230831387426 1104 66-67 0.10506015598490137 1104 68-69 0.05366660063612727 1104 70-71 -0.0026342193359418786 1104 72-73 -0.12822265912234698 1104 74-75 -0.06984512126308928 1104 76-77 -0.2954185470838837 1104 78-79 -0.38046476016363684 1104 80-81 -0.1457837213374127 1104 82-83 -0.20652451608632205 1104 84-85 -0.025244006479550052 1104 86-87 -0.22993442982354395 1104 88-89 -0.052372518524641976 1104 90-91 -0.19462454254207628 1104 92-93 -0.025010435121583185 1104 94-95 0.16591289559125144 1104 96-97 0.09600156373802804 1104 98-99 -0.14490892793351406 1104 100-101 -0.31305438414139886 1105 1 0.15029049988099175 1105 2 0.129108858719313 1105 3 0.19698197301147502 1105 4 0.12371822219878936 1105 5 0.05753238621210954 1105 6 0.0690569211407066 1105 7 0.06824447172921566 1105 8 0.08695982401452795 1105 9 0.13201858475579797 1105 10-11 0.11361014124842228 1105 12-13 0.21052174197913587 1105 14-15 0.32345011326203377 1105 16-17 0.2403656485812249 1105 18-19 0.13532357484408664 1105 20-21 0.19931364879527536 1105 22-23 0.13011091963664967 1105 24-25 0.2760666534974945 1105 26-27 0.20963469737503004 1105 28-29 0.1608069223191535 1105 30-31 0.15975944718299928 1105 32-33 0.2326472181931436 1105 34-35 0.17859479529973044 1105 36-37 0.2432998443481864 1105 38-39 0.22867512982546856 1105 40-41 0.30958917294805843 1105 42-43 0.17910171036540845 1105 44-45 0.10252534364838084 1105 46-47 -0.2634131397140109 1105 48-49 -0.24390050368298688 1105 50-51 -0.40187998217488996 1105 52-53 -0.25553186547518436 1105 54-55 -0.10311070035064063 1105 56-57 -0.040863066731930076 1105 58-59 -0.08939415467083478 1105 60-61 -0.13364732919423972 1105 62-63 0.15839851899399804 1105 64-65 0.05296914777442652 1105 66-67 0.09338898203964874 1105 68-69 -0.034270627699928724 1105 70-71 -0.08735722252227518 1105 72-73 -0.07594893789529777 1105 74-75 -0.24557231041706018 1105 76-77 -0.26985602979110723 1105 78-79 -0.1892519452119643 1105 80-81 -0.06581902091524583 1105 82-83 -0.161134658801938 1105 84-85 -0.38789741014428003 1105 86-87 -0.11737094859792307 1105 88-89 -0.2608479873262439 1105 90-91 -0.28351767095042746 1105 92-93 -0.060747352857053016 1105 94-95 0.055385499370228075 1105 96-97 0.038734134850390234 1105 98-99 -0.020047716412729955 1105 100-101 -0.21345245270558522 1106 1 0.10613855854188614 1106 2 0.16294105008847026 1106 3 0.16468642466832506 1106 4 0.08876410111052024 1106 5 0.03451679940279462 1106 6 -0.020004264097899238 1106 7 0.07964007216196478 1106 8 0.12392740825942639 1106 9 0.1248202035401107 1106 10-11 0.11545731839311202 1106 12-13 -0.001252509733809859 1106 14-15 0.14784707603800484 1106 16-17 0.03530995278445204 1106 18-19 0.2301847360413447 1106 20-21 0.14985057230371268 1106 22-23 0.12672008740520369 1106 24-25 0.16563971917045706 1106 26-27 0.11289630734417244 1106 28-29 0.16169745403369973 1106 30-31 0.32993214422191386 1106 32-33 0.2781304493507406 1106 34-35 0.1591072767678554 1106 36-37 -0.19604883538094953 1106 38-39 0.02043595845070456 1106 40-41 0.2530469202734551 1106 42-43 0.3832979278594948 1106 44-45 0.3666095685151447 1106 46-47 0.0730906467987893 1106 48-49 0.17595564889946047 1106 50-51 0.3740426046744929 1106 52-53 0.29454526719300844 1106 54-55 -0.054560193478820906 1106 56-57 0.2908695133057364 1106 58-59 0.28424650726769585 1106 60-61 -0.016485811383610383 1106 62-63 0.05613562681945439 1106 64-65 -0.3537303754054406 1106 66-67 0.06828380112848009 1106 68-69 -0.5920069341470153 1106 70-71 -0.3558850904551214 1106 72-73 -0.08895731229927506 1106 74-75 0.14840132423454122 1106 76-77 0.12545189984787086 1106 78-79 0.12310430754277135 1106 80-81 -0.5192479186192962 1106 82-83 -0.1395525375533886 1106 84-85 0.004868146613343782 1106 86-87 0.2768681426390067 1106 88-89 0.3012408053972422 1106 90-91 0.20557657114597916 1106 92-93 0.10898057918606341 1106 94-95 0.08282269134041442 1106 96-97 0.03309443456589989 1106 98-99 0.19497712631676478 1106 100-101 -0.14713839252206995 1107 1 0.1727430625118238 1107 2 0.2254673044092499 1107 3 0.2543419868168897 1107 4 0.1635232206606858 1107 5 0.0460978981755531 1107 6 0.13787768328985095 1107 7 0.1707121903087625 1107 8 0.13485862429363493 1107 9 0.2689679623865189 1107 10-11 0.16225923601811587 1107 12-13 0.322313745340459 1107 14-15 0.4418681548433554 1107 16-17 0.30030011035747606 1107 18-19 0.34206534598926197 1107 20-21 0.25540754064783044 1107 22-23 0.3176160779112678 1107 24-25 0.3587556714370379 1107 26-27 0.40973448654227695 1107 28-29 0.45020394815179543 1107 30-31 0.40733412024217586 1107 32-33 0.35772224764220795 1107 34-35 0.4011452713615924 1107 36-37 0.4375614045105465 1107 38-39 0.35235273578580717 1107 40-41 0.36040943239989076 1107 42-43 0.429378381458811 1107 44-45 0.34463599707984827 1107 46-47 0.32530637019362985 1107 48-49 0.2652024442240659 1107 50-51 0.2906193238027086 1107 52-53 0.4796620106211833 1107 54-55 0.44081810420514245 1107 56-57 0.3873281233101622 1107 58-59 0.25688738583381365 1107 60-61 0.2572561004018752 1107 62-63 0.3079293973637576 1107 64-65 0.33120835666391457 1107 66-67 0.4041290725251656 1107 68-69 0.33809959011055923 1107 70-71 0.4817205154898936 1107 72-73 0.3683585778046172 1107 74-75 0.3983791905470717 1107 76-77 0.41268311682393843 1107 78-79 0.5238945272784861 1107 80-81 0.3899276721617859 1107 82-83 0.2774696809013939 1107 84-85 0.5350829846606793 1107 86-87 0.44317125171570027 1107 88-89 0.2735623937483318 1107 90-91 0.3953159587492294 1107 92-93 0.32565430979553867 1107 94-95 0.1328777900943372 1107 96-97 0.059725498960801815 1107 98-99 0.050929204528738836 1107 100-101 -0.3428519920247659 1108 1 0.1926633812369225 1108 2 0.16899335282720074 1108 3 0.18642598774029295 1108 4 0.15637807015455252 1108 5 0.13399423288790047 1108 6 0.05175106290681697 1108 7 0.12443875993239573 1108 8 0.19177956456641 1108 9 0.27113484744805305 1108 10-11 0.13457692631634188 1108 12-13 0.1874841865020116 1108 14-15 0.3003248415607658 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-0.7927024514919836 2316 72-73 -0.7093075861270428 2316 74-75 -0.5333825953634452 2316 76-77 -0.49259637937329614 2316 78-79 -0.5933203096329436 2316 80-81 -0.6263736774370265 2316 82-83 -0.31849987471199626 2316 84-85 -0.3437915228925803 2316 86-87 -0.3092720738096695 2316 88-89 -0.13695995015356033 2316 90-91 -0.22073435701729593 2316 92-93 -0.1469788871764841 2316 94-95 -0.18576611557833544 2316 96-97 -0.06677102749618413 2316 98-99 0.008508435344168674 2316 100-101 -0.03490316199591348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 7.0 4 27.0 5 54.0 6 130.0 7 353.0 8 792.0 9 1496.0 10 2611.0 11 4371.0 12 6005.0 13 8505.0 14 10942.0 15 14862.0 16 19656.0 17 25111.0 18 22658.0 19 16110.0 20 15007.0 21 15298.0 22 16786.0 23 19249.0 24 22569.0 25 26396.0 26 31545.0 27 38261.0 28 46737.0 29 56347.0 30 67895.0 31 81598.0 32 99661.0 33 122712.0 34 157050.0 35 206561.0 36 291942.0 37 409872.0 38 267745.0 39 14365.0 >>END_MODULE >>Per base sequence content fail #Base 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20.494406653173268 18-19 19.580776857358213 28.896662485394348 31.823420791353268 19.699139865894175 20-21 20.90887566116563 28.27558943330528 31.387329343845355 19.42820556168373 22-23 20.259922271299505 28.80839809946696 30.995089884901372 19.936589744332164 24-25 19.898962774775953 28.885508268380278 31.636466802721724 19.57906215412205 26-27 19.880385339392763 29.138109839894955 31.313647892195018 19.667856928517267 28-29 19.56071889240805 29.041574860808467 31.398023811769892 19.999682435013593 30-31 20.298660529567634 28.616644888558042 31.537845807254865 19.54684877461946 32-33 19.680342820742972 28.852506942064004 31.535744274256594 19.93140596293643 34-35 20.01079364590069 29.03857385625769 31.488885603933912 19.46174689390771 36-37 19.939419723895536 28.66607107581361 31.01393038125131 20.380578819039542 38-39 19.650964603983113 29.088127748001074 31.200923027131083 20.059984620884727 40-41 20.416357745409965 28.479663737995853 31.380227093610436 19.72375142298374 42-43 19.784647902847077 28.78331412367533 31.210876277971533 20.22116169550606 44-45 19.97377285593331 28.928222340427023 30.996832287781263 20.10117251585841 46-47 20.470402476259682 28.70911234048197 30.116368887224265 20.70411629603408 48-49 20.183146265762662 28.80653007013516 30.32542471981895 20.684898944283223 50-51 19.980698586929215 29.31323302638498 29.87142354109244 20.834644845593363 52-53 20.044585190076656 29.485558732243213 29.921019719851643 20.54883635782849 54-55 20.312250442956454 29.047599255403505 29.80487499614719 20.835275305492846 56-57 20.478014695786754 29.22071887372776 29.233631626483795 21.067634804001692 58-59 20.227138356526474 29.17273387026723 29.493941513869647 21.106186259336642 60-61 20.32598045854516 29.09551420776409 29.593227272854637 20.98527806083611 62-63 20.165399987110597 28.969889235200768 29.71677406278633 21.147936714902297 64-65 20.130911495572303 29.062590190791177 29.298008587330838 21.508489726305683 66-67 20.121954351879914 29.28307802808864 29.034186353759157 21.560781266272294 68-69 20.286366700595043 28.831857460658377 29.341903925954416 21.539871912792165 70-71 20.342526768295315 28.711771044824562 29.30834998021055 21.63735220666957 72-73 20.406679325678773 28.633317050344324 29.128951932803247 21.831051691173656 74-75 20.172288345271596 28.91020569804987 28.962206964573756 21.955298992104773 76-77 19.920711495011894 28.773582702795597 29.499592302598337 21.806113499594172 78-79 19.767463038704634 28.521772348869423 29.305597456491263 22.405167155934684 80-81 19.834901408792742 28.78308906973725 29.050524315988767 22.331485205481243 82-83 19.83302487254101 28.8443998604571 29.06249401642104 22.260081250580846 84-85 19.87615263330942 28.367132390303684 29.072629909433285 22.68408506695361 86-87 19.498953409195988 28.91275981464928 28.886980335674966 22.701306440479758 88-89 19.946224067940275 28.412115579725793 28.826818381888508 22.81484197044542 90-91 19.7413759284444 28.421583810320865 29.08127904891426 22.755761212320476 92-93 19.653944091655777 28.53207613696996 29.037569949644425 22.776409821729835 94-95 19.56501992837507 28.542257285140966 28.98047371833894 22.912249068145023 96-97 19.844752752307716 28.259034023352235 28.692159974295915 23.204053250044133 98-99 19.63820916572584 28.076126918671783 28.60361207074699 23.682051844855383 100-101 19.443593453304402 28.365745198931116 29.00204269007432 23.188618657690164 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 264.0 1 166.5 2 93.5 3 118.5 4 108.5 5 119.5 6 202.5 7 443.5 8 735.5 9 872.0 10 864.5 11 894.0 12 1051.5 13 1191.5 14 1387.0 15 1644.5 16 1904.5 17 2228.5 18 2665.5 19 3207.0 20 3793.5 21 4485.0 22 5431.0 23 6505.5 24 7868.0 25 10135.5 26 13898.5 27 18417.0 28 21759.5 29 26062.5 30 33020.0 31 40650.5 32 49860.0 33 60028.0 34 71529.5 35 82636.5 36 91711.5 37 98023.5 38 96366.5 39 92804.0 40 91887.0 41 91059.5 42 89270.5 43 89284.0 44 90158.5 45 87697.0 46 82457.5 47 77610.0 48 75967.5 49 73139.5 50 67721.0 51 61052.0 52 53461.0 53 46240.0 54 40053.5 55 34293.5 56 28852.5 57 23799.5 58 19296.5 59 15530.5 60 12196.0 61 9398.0 62 7053.0 63 4868.0 64 3123.5 65 2237.0 66 1675.0 67 1306.5 68 1034.5 69 853.5 70 746.0 71 583.0 72 477.5 73 417.0 74 342.0 75 288.5 76 220.5 77 157.0 78 122.0 79 105.5 80 97.5 81 65.0 82 33.5 83 18.5 84 11.0 85 6.5 86 4.0 87 2.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.604087995389704E-4 2 0.04726114209445317 3 0.0011208175990779408 4 0.0 5 0.08803088226091327 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.868029331796568E-4 26-27 0.007869073560193042 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010250810958233665 36-37 1.401021998847426E-4 38-39 0.0036660075636507643 40-41 0.004973628095908362 42-43 4.6700733294914197E-4 44-45 4.67007332949142E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.67007332949142E-5 68-69 8.639635659559127E-4 70-71 7.00510999423713E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0013076205322575974 82-83 7.939124660135413E-4 84-85 0.005137080662440561 86-87 0.0026152410645151948 88-89 7.00510999423713E-5 90-91 0.003642657197003308 92-93 0.0010040657658406554 94-95 2.33503666474571E-5 96-97 0.0 98-99 7.00510999423713E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2141294.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.812461427416956 #Duplication Level Percentage of deduplicated Percentage of total 1 75.23460379641791 41.23793818598154 2 14.412574208698123 15.799773357681007 3 4.925120221238774 8.098738864561247 4 2.137381107136716 4.686204779624839 5 1.0865762125211964 2.977895836838344 6 0.5859560227349281 1.9270615136592542 7 0.3604780936197641 1.383108412137348 8 0.23907987814061482 1.0483645278923197 9 0.15629775179550318 0.7710358042334706 >10 0.6923057572222174 7.135520387650117 >50 0.08941023727865556 3.462822026176292 >100 0.07416657011475167 7.995097243574584 >500 0.004942370404222929 1.834810635410104 >1k 8.521328283142924E-4 0.6261607152790705 >5k 2.556398484942877E-4 1.0154677093004827 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9142 0.4269381037821056 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7448 0.3478270615805209 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5151 0.24055547720210302 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.6700733294914194E-5 0.0 2 0.0 0.0 0.0 4.6700733294914194E-5 0.0 3 0.0 0.0 0.0 4.6700733294914194E-5 0.0 4 0.0 0.0 0.0 4.6700733294914194E-5 0.0 5 0.0 0.0 0.0 4.6700733294914194E-5 0.0 6 0.0 0.0 0.0 4.6700733294914194E-5 0.0 7 0.0 0.0 0.0 4.6700733294914194E-5 0.0 8 0.0 0.0 0.0 4.6700733294914194E-5 0.0 9 0.0 0.0 0.0 4.6700733294914194E-5 0.0 10-11 0.0 0.0 0.0 4.6700733294914194E-5 0.0 12-13 0.0 0.0 0.0 4.6700733294914194E-5 0.0 14-15 0.0 0.0 0.0 9.340146658982839E-5 0.0 16-17 0.0 0.0 0.0 1.1675183323728549E-4 0.0 18-19 0.0 0.0 0.0 1.401021998847426E-4 0.0 20-21 0.0 0.0 0.0 2.8020439976948514E-4 0.0 22-23 0.0 0.0 0.0 0.0011208175990779408 0.0 24-25 0.0 0.0 0.0 0.00217158409821351 0.0 26-27 0.0 0.0 0.0 0.00378275939688805 0.0 28-29 0.0 0.0 0.0 0.010717818291182808 0.0 30-31 0.0 0.0 0.0 0.035259053637660215 0.0 32-33 0.0 0.0 0.0 0.08707351722836752 0.0 34-35 0.0 0.0 0.0 0.16721197556244027 0.0 36-37 0.0 0.0 0.0 0.29746032072195594 0.0 38-39 0.0 0.0 0.0 0.47882261847275526 0.0 40-41 0.0 0.0 0.0 0.6697819169156594 0.0 42-43 0.0 0.0 0.0 0.8637534126560855 2.3350366647457097E-5 44-45 0.0 0.0 0.0 1.1098662771202834 4.6700733294914194E-5 46-47 0.0 0.0 0.0 1.39070113678925 4.6700733294914194E-5 48-49 0.0 0.0 0.0 1.673310624323423 4.6700733294914194E-5 50-51 0.0 0.0 0.0 1.9644196453172706 4.6700733294914194E-5 52-53 0.0 0.0 0.0 2.260058637440725 4.6700733294914194E-5 54-55 0.0 0.0 0.0 2.5880612377375547 4.6700733294914194E-5 56-57 0.0 0.0 0.0 2.9725950756878783 4.6700733294914194E-5 58-59 0.0 0.0 0.0 3.3845422440823167 4.6700733294914194E-5 60-61 0.0 0.0 0.0 3.8085849024001375 4.6700733294914194E-5 62-63 0.0 0.0 0.0 4.229148356087487 4.6700733294914194E-5 64-65 0.0 0.0 0.0 4.671404300390325 4.6700733294914194E-5 66-67 0.0 0.0 0.0 5.179765132672113 4.6700733294914194E-5 68-69 0.0 0.0 0.0 5.733939384316212 4.6700733294914194E-5 70-71 0.0 0.0 0.0 6.325614324796128 4.6700733294914194E-5 72-73 0.0 0.0 0.0 6.906524746251566 4.6700733294914194E-5 74-75 0.0 0.0 0.0 7.5127469651528465 4.6700733294914194E-5 76-77 0.0 0.0 0.0 8.18460706470013 4.6700733294914194E-5 78-79 0.0 0.0 0.0 8.907487715372106 4.6700733294914194E-5 80-81 0.0 0.0 0.0 9.665440616748564 4.6700733294914194E-5 82-83 0.0 0.0 0.0 10.417836130862927 4.6700733294914194E-5 84-85 0.0 0.0 0.0 11.186717003830395 4.6700733294914194E-5 86-87 0.0 0.0 0.0 12.006128070222958 4.6700733294914194E-5 88-89 0.0 0.0 0.0 12.86413729268377 4.6700733294914194E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5655 0.0 45.66615 1 GTATCAA 9375 0.0 36.271202 1 TATCAAC 11920 0.0 28.407362 2 TCAACGC 11915 0.0 28.324884 4 CAACGCA 12040 0.0 28.030815 5 AACGCAG 12295 0.0 27.466288 6 ATCAACG 12600 0.0 26.785 3 CGCAGAG 13925 0.0 24.14888 8 ACGCAGA 13965 0.0 24.147732 7 ACGCCGA 160 1.8620995E-6 23.748102 6 CGCCGAA 160 1.8620995E-6 23.748102 7 GTACATG 9310 0.0 23.549324 1 GAGTACT 8365 0.0 22.626705 12-13 TACATGG 9650 0.0 22.32534 2 GCAGAGT 14920 0.0 22.09274 9 CTACACG 345 0.0 22.044727 4 CTCCGAC 325 0.0 21.921326 94-95 AGTACTT 8775 0.0 21.596565 12-13 CATTCCG 470 0.0 21.221708 9 ACATGGG 9700 0.0 21.120752 3 >>END_MODULE