FastQCFastQC Report
Thu 26 May 2016
SRR938525_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938525_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140875
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100760.47064868336544635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75860.3543410988497694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51560.24083610673206046No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53900.044.6461561
GTATCAA91350.037.8190771
TCAACGC115550.029.5706734
TATCAAC117050.029.3541242
CAACGCA117850.028.9532385
ATCAACG121100.028.3331783
AACGCAG120400.028.2176536
ACGCAGA134850.025.0119347
CGCAGAG135250.024.832598
GTACATG90700.024.5878221
TACATGG94600.023.1585872
TAGGACG23050.022.885184
GCAGAGT142850.022.8779759
GGACGTG21400.022.8699286
ACATGGG94100.022.8271183
GAGTACT83900.022.53307212-13
GACGTGG11150.022.1548277
GTGGTAT29500.021.4837991
AGGACGT24500.021.1428075
GTACTTT92350.020.65180814-15
Produced by FastQC (version 0.11.5)