FastQCFastQC Report
Thu 26 May 2016
SRR938525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938525_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140875
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96980.4529923512582472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77300.3610673206048929No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53540.2500846616453553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53750.047.071791
GTATCAA93850.039.171731
TCAACGC117100.030.9359254
TATCAAC118350.030.7411922
CAACGCA119500.030.3544035
AACGCAG122500.029.5439016
ATCAACG123400.029.3565373
CTTAACG705.8145815E-727.14165320-21
ACGCAGA139300.025.878537
CGCAGAG138800.025.834888
GTACATG95950.023.7916071
GCAGAGT147750.023.6591599
GAGTACT86100.023.30625712-13
TACATGG98550.022.970892
ATGGGCG2755.0931703E-1122.4737595
ACATGGG100050.022.1428683
GTACTTT93000.021.62815514-15
TAGATCG1353.7755532E-421.1291754
TAGGACG20750.021.078264
AGTACTT90300.020.9073212-13
Produced by FastQC (version 0.11.5)