FastQCFastQC Report
Thu 26 May 2016
SRR938524_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938524_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140479
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96070.4488247723990752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73990.34567029155623574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49800.2326582040748823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48400.046.2292631
GTATCAA84700.039.484271
TCAACGC109000.030.607054
CAACGCA110500.030.191575
TATCAAC111250.030.0734672
AACGCAG114800.029.1387256
ATCAACG115350.028.9633393
GTACATG90900.025.3496951
ACGCAGA131600.025.3058367
CGCAGAG131350.025.2816648
TACATGG93400.024.2707042
GCAGAGT138800.023.3064239
ACATGGG96350.022.9850273
TAGGACG21000.022.404064
GAGTACT81100.022.19837212-13
GTGTAGC14300.022.018991
TGTAGGA22650.020.9817942
GACGTGG11800.020.9353337
AGTACTT86300.020.83328812-13
GGACGTG20550.020.8099216
Produced by FastQC (version 0.11.5)