FastQCFastQC Report
Thu 26 May 2016
SRR938524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140479
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91470.427334255556817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74750.34922089868669587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52640.24592626229923303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49150.044.951451
GTATCAA86300.039.6402171
TCAACGC111850.030.4477674
TATCAAC113500.030.0518952
CAACGCA113600.029.978725
CGTCGAT404.493886E-429.68950740-41
AACGCAG117250.029.0454796
ATCAACG118700.028.7720453
GTACATG89150.026.0616441
CGCAGAG133500.025.3676668
ACGCAGA134400.025.2684757
TACATGG92800.024.776542
ACATGGG92600.024.2116033
ATAGCCC12250.023.6530043
GCAGAGT141900.023.4643159
CTCCGAC4700.023.24408194-95
CATTCCG5150.022.1348349
CTGATCG1100.00287898921.5898489
AGTACTT89250.021.50034312-13
GAGTACT83900.021.31450712-13
Produced by FastQC (version 0.11.5)