##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938523_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 394950 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.22359792378782 31.0 31.0 33.0 30.0 34.0 2 31.00642359792379 31.0 31.0 34.0 28.0 34.0 3 30.94685656412204 31.0 31.0 34.0 27.0 34.0 4 34.679559437903535 37.0 35.0 37.0 32.0 37.0 5 34.76806937587036 37.0 35.0 37.0 32.0 37.0 6 34.9380529180909 37.0 35.0 37.0 32.0 37.0 7 34.899255601974936 37.0 35.0 37.0 32.0 37.0 8 34.946231168502344 37.0 35.0 37.0 32.0 37.0 9 36.30331687555387 39.0 35.0 39.0 32.0 39.0 10-11 36.170348145334856 39.0 35.0 39.0 31.5 39.0 12-13 36.001078617546526 38.5 35.0 39.0 30.5 39.0 14-15 36.52682364856311 39.0 36.0 41.0 30.5 41.0 16-17 36.53949993670085 38.5 36.0 40.0 30.5 41.0 18-19 36.98581845803266 39.0 36.0 41.0 31.0 41.0 20-21 37.116294467654136 39.0 36.0 41.0 31.5 41.0 22-23 36.97272692745917 39.0 35.5 41.0 31.5 41.0 24-25 36.728945436131156 39.0 35.0 41.0 31.0 41.0 26-27 36.31121281174832 38.5 35.0 41.0 30.0 41.0 28-29 35.91966324851247 38.0 35.0 40.0 30.0 41.0 30-31 35.34623749841752 37.5 35.0 40.0 29.0 41.0 32-33 34.640659577161664 35.5 34.0 40.0 27.5 41.0 34-35 34.19737055323459 35.0 34.0 39.0 25.5 41.0 36-37 33.7108444106849 35.0 33.0 39.0 24.0 41.0 38-39 33.05922648436511 35.0 33.0 39.0 21.0 41.0 40-41 32.66892011647044 35.0 33.0 39.0 19.5 41.0 42-43 32.110707684517024 35.0 33.0 39.0 15.5 41.0 44-45 31.284674009368274 35.0 31.0 38.0 5.5 40.0 46-47 30.290360805165214 35.0 29.5 38.0 2.0 40.0 48-49 29.536572983922014 35.0 27.0 38.0 2.0 40.0 50-51 28.67198379541714 34.0 25.5 37.5 2.0 39.5 52-53 28.653987846562856 34.5 25.5 37.5 2.0 40.0 54-55 28.775905810862135 35.0 26.0 38.0 2.0 40.0 56-57 28.483557412330676 35.0 24.5 38.0 2.0 40.0 58-59 28.147459172047093 35.0 24.5 37.5 2.0 40.0 60-61 27.604394227117357 34.5 22.5 36.5 2.0 40.0 62-63 26.978565641220406 34.0 21.5 36.0 2.0 39.5 64-65 26.39198759336625 33.0 19.5 35.5 2.0 39.0 66-67 25.868031396379287 33.0 14.5 35.0 2.0 39.0 68-69 25.478380807697178 33.0 8.5 35.0 2.0 38.0 70-71 24.864608178250414 33.0 2.0 35.0 2.0 37.5 72-73 24.235813394100518 32.5 2.0 35.0 2.0 37.0 74-75 23.65107481959742 31.5 2.0 35.0 2.0 36.0 76-77 22.928926446385617 31.0 2.0 35.0 2.0 35.5 78-79 22.213730852006584 30.5 2.0 35.0 2.0 35.0 80-81 21.599525256361567 29.5 2.0 34.5 2.0 35.0 82-83 20.96727813647297 29.0 2.0 34.0 2.0 35.0 84-85 20.311297632611723 28.5 2.0 34.0 2.0 35.0 86-87 19.608436510950753 27.0 2.0 34.0 2.0 35.0 88-89 18.799897455374097 25.5 2.0 33.5 2.0 35.0 90-91 17.904454994303077 23.0 2.0 33.0 2.0 35.0 92-93 17.120248132675023 19.0 2.0 33.0 2.0 35.0 94-95 16.332438283327 6.5 2.0 33.0 2.0 35.0 96-97 15.305148753006709 2.0 2.0 32.5 2.0 35.0 98-99 14.201254589188505 2.0 2.0 31.5 2.0 35.0 100-101 11.59613748575769 2.0 2.0 26.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49417491206854436 1101 2 0.6526600909290252 1101 3 0.503491045654652 1101 4 0.2033922116989899 1101 5 0.32801981128753965 1101 6 0.3689469588297811 1101 7 0.5494054691034052 1101 8 0.7346379226425341 1101 9 0.6897448590128619 1101 10-11 0.8424953707460503 1101 12-13 1.0267419845286625 1101 14-15 1.1116705393802917 1101 16-17 1.2203619763114517 1101 18-19 1.31759013693474 1101 20-21 1.0717654195627162 1101 22-23 1.2529433051529608 1101 24-25 1.3365371152735577 1101 26-27 1.4025409889127758 1101 28-29 1.3325423196082653 1101 30-31 1.2397974753965784 1101 32-33 0.9995919240306606 1101 34-35 0.9197675658828643 1101 36-37 0.8250251324530282 1101 38-39 0.9932727904423331 1101 40-41 1.1803100970376192 1101 42-43 1.4056442231269344 1101 44-45 1.4030932343309388 1101 46-47 1.466681935236366 1101 48-49 0.7448647304204279 1101 50-51 0.803297978703668 1101 52-53 0.6153950618541053 1101 54-55 0.7604983397448457 1101 56-57 0.5225343706550198 1101 58-59 0.8314939024598687 1101 60-61 1.1567443257541932 1101 62-63 1.1249126309520747 1101 64-65 1.106262510122498 1101 66-67 1.1013293469436718 1101 68-69 1.008587439639001 1101 70-71 1.102621776126906 1101 72-73 1.375082632291214 1101 74-75 1.5635927991546161 1101 76-77 1.3134133790002451 1101 78-79 1.6871098865168612 1101 80-81 1.751705278060797 1101 82-83 1.0971131625268917 1101 84-85 0.8623437418953834 1101 86-87 1.1654512855665615 1101 88-89 0.801724684876735 1101 90-91 0.5539157603068219 1101 92-93 0.9061343069931098 1101 94-95 0.8850398830466908 1101 96-97 1.2000540464975469 1101 98-99 1.404678658775655 1101 100-101 1.3062476732055597 1102 1 0.2343649192083852 1102 2 0.3431699337068572 1102 3 0.3927579736297062 1102 4 0.13323965228533297 1102 5 0.15192807164395106 1102 6 0.34128372608560653 1102 7 0.24666901658333273 1102 8 0.4061251947869664 1102 9 0.3288332910168208 1102 10-11 0.5071487971401396 1102 12-13 0.5255180121554659 1102 14-15 0.7564198436102814 1102 16-17 0.8179227742248685 1102 18-19 0.6480371782729435 1102 20-21 0.5434938434068641 1102 22-23 0.5727097304250819 1102 24-25 0.30922358892172497 1102 26-27 0.4294501672484685 1102 28-29 0.4015947609960122 1102 30-31 0.5716761272784296 1102 32-33 0.6192716512596732 1102 34-35 0.5186179061180525 1102 36-37 0.9260283526660587 1102 38-39 1.0392591810352272 1102 40-41 1.1573424011656144 1102 42-43 1.465152594223703 1102 44-45 1.6395129694283384 1102 46-47 1.5224018204161034 1102 48-49 1.7497314777138442 1102 50-51 1.9677729091266656 1102 52-53 1.3841152821691303 1102 54-55 1.7215352191981381 1102 56-57 1.8633778916155457 1102 58-59 2.0016479189834975 1102 60-61 1.705080306170636 1102 62-63 2.217369901202261 1102 64-65 1.6823491498812047 1102 66-67 1.3277095943609467 1102 68-69 1.502296367351775 1102 70-71 1.718319950368116 1102 72-73 1.4549104018929846 1102 74-75 1.2206656109397223 1102 76-77 1.1388698186977493 1102 78-79 0.7697061650580039 1102 80-81 0.7450863560307539 1102 82-83 1.172256323581255 1102 84-85 1.155984185439653 1102 86-87 1.2266462614513927 1102 88-89 1.2677120954466083 1102 90-91 0.5543237510978827 1102 92-93 0.5176797178252635 1102 94-95 0.9844475678446649 1102 96-97 0.6903496941100737 1102 98-99 0.9523953674556598 1102 100-101 0.719782767699142 1103 1 0.5526302935399343 1103 2 0.5523109764876075 1103 3 0.4688776260361216 1103 4 0.4634257039787357 1103 5 0.6038415074728292 1103 6 0.6457762617362306 1103 7 0.6305816725548112 1103 8 0.6960650343591581 1103 9 0.6221571546531877 1103 10-11 0.8515602833255258 1103 12-13 0.7673364724941507 1103 14-15 0.9559101510969086 1103 16-17 0.858677286256956 1103 18-19 1.1551951551000457 1103 20-21 1.035771862569078 1103 22-23 1.092442907786939 1103 24-25 1.1952947787612942 1103 26-27 0.9449846480862618 1103 28-29 1.0739989499443183 1103 30-31 0.9712277092748636 1103 32-33 1.1532093740760772 1103 34-35 1.2374723513052146 1103 36-37 1.2748683147872697 1103 38-39 1.1392480402152714 1103 40-41 1.116497369971313 1103 42-43 0.8892209431541787 1103 44-45 1.0227571762019636 1103 46-47 0.74021877674409 1103 48-49 0.6771351096211511 1103 50-51 1.1608990232086605 1103 52-53 1.61782183297127 1103 54-55 1.4430773872017006 1103 56-57 1.9254670738793642 1103 58-59 2.159931118626993 1103 60-61 2.144028612766 1103 62-63 2.2239986890810464 1103 64-65 2.183553751039849 1103 66-67 1.7229162565712883 1103 68-69 2.136564136959617 1103 70-71 2.2274763117763143 1103 72-73 2.1725323938715952 1103 74-75 1.9743699335778722 1103 76-77 2.0503002999811706 1103 78-79 1.8588536138102292 1103 80-81 2.156064241503124 1103 82-83 2.5015998507648547 1103 84-85 2.3048533922132712 1103 86-87 2.1333001161751 1103 88-89 1.9255148472291381 1103 90-91 1.987172458762771 1103 92-93 1.7462406024063704 1103 94-95 1.6756031493954673 1103 96-97 1.1322712070334244 1103 98-99 0.9585785225358734 1103 100-101 0.5728980194816735 1104 1 0.15174526847727776 1104 2 0.0468676807482602 1104 3 0.3465796937052943 1104 4 0.3449654909461586 1104 5 0.20540400747282916 1104 6 0.35710536574625706 1104 7 0.23177214874529284 1104 8 0.3856796960132911 1104 9 0.3917177749539391 1104 10-11 0.6685147789395529 1104 12-13 0.4663594204891339 1104 14-15 0.6048175759089389 1104 16-17 0.7311416502920451 1104 18-19 0.8889435886840005 1104 20-21 0.9071201551755905 1104 22-23 0.7711905581628784 1104 24-25 0.972452516856535 1104 26-27 0.980610822898285 1104 28-29 0.6672790251322951 1104 30-31 0.7435295576457932 1104 32-33 0.5618285783367227 1104 34-35 0.5846586764931843 1104 36-37 0.8881495647872768 1104 38-39 1.219683503874414 1104 40-41 1.156186436387351 1104 42-43 1.046244361073981 1104 44-45 0.9381707100365446 1104 46-47 1.3434651739871946 1104 48-49 0.9835848276662666 1104 50-51 1.0508582963916204 1104 52-53 0.8048127477582376 1104 54-55 1.2276266509861635 1104 56-57 1.4200335682402638 1104 58-59 1.034108750205938 1104 60-61 0.8319985375780341 1104 62-63 1.1888326489807959 1104 64-65 1.1225907967791962 1104 66-67 1.5962519395286776 1104 68-69 1.5571762140272902 1104 70-71 1.907016960272557 1104 72-73 1.6213693299618193 1104 74-75 1.2903552092670942 1104 76-77 1.6544669666478384 1104 78-79 1.519910553033288 1104 80-81 1.163729889999363 1104 82-83 0.9740602987598415 1104 84-85 1.011032903491472 1104 86-87 1.0390214507615667 1104 88-89 0.845382876677764 1104 90-91 0.7950123710434696 1104 92-93 0.7355204426319304 1104 94-95 1.0533933279668979 1104 96-97 1.1940956901161304 1104 98-99 1.3538636102551713 1104 100-101 1.092566330885182 1105 1 0.3693916571762976 1105 2 0.2873172896189189 1105 3 0.24302535330884822 1105 4 0.28713151205954546 1105 5 0.3619696640384831 1105 6 0.3570294183018916 1105 7 0.2959857129588528 1105 8 0.2872897818339055 1105 9 0.477823190006724 1105 10-11 0.47699431110329726 1105 12-13 0.7062963841103098 1105 14-15 0.6693729036221612 1105 16-17 0.5162293695902846 1105 18-19 0.5309842965141769 1105 20-21 0.6803031125690708 1105 22-23 0.6496556603121846 1105 24-25 0.6825896019936195 1105 26-27 0.5931459485913066 1105 28-29 0.4840368287322079 1105 30-31 0.6624366739213343 1105 32-33 0.6356115205407136 1105 34-35 0.90141648009309 1105 36-37 0.492947544585256 1105 38-39 0.7008010705183025 1105 40-41 0.9421760315874721 1105 42-43 1.4016499204269124 1105 44-45 1.5480097014544896 1105 46-47 1.1459163777541868 1105 48-49 1.1473844783080231 1105 50-51 0.732553063612702 1105 52-53 0.6940528935773322 1105 54-55 1.0196635614441298 1105 56-57 1.3579560258995649 1105 58-59 1.3479046034754774 1105 60-61 1.2450387137761005 1105 62-63 0.8928749517073129 1105 64-65 1.3733028040701498 1105 66-67 1.1912716858642156 1105 68-69 1.6197948819091152 1105 70-71 1.4853282688470237 1105 72-73 1.3833120655887647 1105 74-75 1.5793257416586748 1105 76-77 1.835022522203392 1105 78-79 1.7164530456284162 1105 80-81 1.372091703624335 1105 82-83 1.419805343189097 1105 84-85 1.4331677861526693 1105 86-87 1.4588209495084286 1105 88-89 1.1967822083402488 1105 90-91 1.0062696809849925 1105 92-93 1.1328015241235434 1105 94-95 0.9644683640419309 1105 96-97 0.832358012588978 1105 98-99 1.220889128596479 1105 100-101 0.22461045635036037 1106 1 0.20076808126372114 1106 2 0.26830362354643 1106 3 0.18966566951438324 1106 4 0.15637380713304339 1106 5 0.32560861105339 1106 6 -0.0633896056976937 1106 7 0.4469712718728047 1106 8 0.08840838474279167 1106 9 0.23466914314423803 1106 10-11 0.28510741141588625 1106 12-13 0.099454306040613 1106 14-15 0.39255097590509536 1106 16-17 0.23980140710094844 1106 18-19 -0.02965405632366469 1106 20-21 0.14364497105160012 1106 22-23 0.17739468741182662 1106 24-25 0.17681252172805273 1106 26-27 0.16817371991064078 1106 28-29 0.007849120447311009 1106 30-31 -0.17454568160322026 1106 32-33 -0.1800773373476261 1106 34-35 -0.5278829076461946 1106 36-37 -0.17749408397657618 1106 38-39 -0.5764115932033249 1106 40-41 -0.6345083312222073 1106 42-43 -1.11750260224224 1106 44-45 -0.93866652371279 1106 46-47 -0.9159846013462776 1106 48-49 -1.0471762181708328 1106 50-51 -1.2201485044980735 1106 52-53 -1.5522948550082702 1106 54-55 -1.3583299284419432 1106 56-57 -1.7800189928299304 1106 58-59 -1.7701493374684887 1106 60-61 -1.033294490388304 1106 62-63 -1.1036600918226185 1106 64-65 -0.9905757513045685 1106 66-67 -1.1420067506750904 1106 68-69 -0.8014120725459222 1106 70-71 -0.6743212958536908 1106 72-73 -0.6490189168700944 1106 74-75 -0.2254515711109626 1106 76-77 -0.6132227179216407 1106 78-79 -0.44884765963393036 1106 80-81 -0.47643189553865284 1106 82-83 -0.6447629742564551 1106 84-85 -0.18434364529738545 1106 86-87 -0.2986385560329161 1106 88-89 0.19639147233570142 1106 90-91 0.6332588824626839 1106 92-93 0.6550314964813921 1106 94-95 -0.11430853538714203 1106 96-97 0.29683655763018457 1106 98-99 0.2513374739425238 1106 100-101 -0.08107749735549596 1107 1 0.47488362283775487 1107 2 0.33011817987743797 1107 3 0.3426291393533134 1107 4 0.3583888799270838 1107 5 0.154556549845708 1107 6 0.3897292984594003 1107 7 0.16639684608185235 1107 8 0.12213948956497234 1107 9 0.2784260711180764 1107 10-11 0.5428404235595821 1107 12-13 0.27853817245379275 1107 14-15 0.4229009198620375 1107 16-17 0.5684892565959387 1107 18-19 0.290094771726352 1107 20-21 0.4680351545384056 1107 22-23 0.3485024820403666 1107 24-25 0.464381491836356 1107 26-27 0.554545659991021 1107 28-29 0.5654991517199477 1107 30-31 0.533343074488748 1107 32-33 0.40117270135613836 1107 34-35 0.18182956982821707 1107 36-37 0.6791100167646746 1107 38-39 0.7396061919505357 1107 40-41 0.7945832759438716 1107 42-43 0.5148735611929212 1107 44-45 0.28768857248928725 1107 46-47 0.0672024833623297 1107 48-49 0.30876217782131476 1107 50-51 0.6454379659043852 1107 52-53 0.6071151138429407 1107 54-55 0.6303283204139696 1107 56-57 0.5226630232336795 1107 58-59 0.575122301032156 1107 60-61 0.01892772495324735 1107 62-63 0.2596877528421473 1107 64-65 -0.03676946829025596 1107 66-67 -0.04441753075720811 1107 68-69 0.09256128687890808 1107 70-71 -0.04629853184757238 1107 72-73 -0.1043857356683553 1107 74-75 0.002984239469718375 1107 76-77 -0.12600115280333313 1107 78-79 -0.12143858251745243 1107 80-81 0.31695192814557416 1107 82-83 0.4182003098044049 1107 84-85 0.578978190437649 1107 86-87 0.6940913288466071 1107 88-89 0.5801222675277664 1107 90-91 0.654406619376168 1107 92-93 0.7084612692748138 1107 94-95 0.6817226257877174 1107 96-97 0.7227265893982331 1107 98-99 1.4650101205988264 1107 100-101 1.4867934240418013 1108 1 0.03937387544702631 1108 2 0.15511507184212903 1108 3 -0.02092219058979694 1108 4 0.22925445295999225 1108 5 0.264083714074296 1108 6 0.1438215449473148 1108 7 0.0718000914627197 1108 8 0.21585415416355858 1108 9 0.3037165984184682 1108 10-11 0.2886623360965075 1108 12-13 0.2828627815813505 1108 14-15 0.41318016943431957 1108 16-17 0.6517224421249281 1108 18-19 0.5059108233967038 1108 20-21 0.5865785811917306 1108 22-23 0.42422902229386494 1108 24-25 0.5277558117686283 1108 26-27 0.42426273997704556 1108 28-29 0.4827958122100071 1108 30-31 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62-63 -1.330500103189486 2316 64-65 -0.8095584168345482 2316 66-67 -0.8328280067137399 2316 68-69 -1.061144951204632 2316 70-71 -1.3829743222816546 2316 72-73 -1.3330910964665676 2316 74-75 -1.4238033756008726 2316 76-77 -2.1196876227241432 2316 78-79 -2.619350643436146 2316 80-81 -2.724087479511372 2316 82-83 -2.591338705998428 2316 84-85 -2.8991774048881744 2316 86-87 -2.940937504597848 2316 88-89 -3.029667067022068 2316 90-91 -2.4641409470343323 2316 92-93 -2.9978528456612636 2316 94-95 -2.643294042633517 2316 96-97 -2.567400441517302 2316 98-99 -2.890606260072822 2316 100-101 -2.1657606459772634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2413.0 3 484.0 4 452.0 5 457.0 6 598.0 7 770.0 8 980.0 9 1400.0 10 1919.0 11 2875.0 12 4475.0 13 5891.0 14 7004.0 15 8492.0 16 10415.0 17 12904.0 18 10090.0 19 7042.0 20 7056.0 21 7611.0 22 8307.0 23 8940.0 24 9526.0 25 10272.0 26 11031.0 27 12099.0 28 13343.0 29 14509.0 30 16421.0 31 18389.0 32 21025.0 33 25202.0 34 34042.0 35 44215.0 36 25870.0 37 19997.0 38 8094.0 39 340.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.810757703517446 18.414755505587248 14.450378180731645 12.324108610163655 2 15.003776607339292 26.522935919337748 39.30485078599056 19.168436687332395 3 22.54625099498578 37.04617241012173 28.438087802108104 11.96948879278439 4 10.735743729370427 19.468739384167645 36.260387671184255 33.53512921527767 5 9.239703793872996 45.58911543923717 28.569182389937108 16.60199837695273 6 21.8732417924973 38.48618017223464 19.16528544419608 20.47529259107198 7 22.216425526957586 43.617627468906775 18.623515616887968 15.542431387247666 8 27.219796584071155 29.444658305812755 19.425815993678707 23.909729116437383 9 31.46328059907869 19.229220534197072 19.99756883858861 29.30993002813563 10-11 23.778906795258127 31.294261897433362 21.782521846741577 23.144309460566934 12-13 30.98008169986383 31.11536147439754 24.024066626555623 13.880490199183 14-15 26.021362407481273 29.022229819996078 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40-41 13.248809844947399 33.44541497436053 40.35787604657024 12.947899134121831 42-43 12.507914959146147 34.8914194244495 38.41693218716282 14.183733429241533 44-45 13.299647527119141 34.493345555094145 36.873170534077445 15.33383638370927 46-47 13.577395739163686 34.71685165067523 35.646456435696216 16.059296174464862 48-49 14.334729824001661 34.43469981781051 35.25398278378957 15.976587574398263 50-51 14.391035055556117 35.3929696441775 35.34612423907869 14.869871061187695 52-53 13.317625371439279 35.86821517966457 34.64403234607823 16.17012710281794 54-55 12.276388074705913 34.913657214266095 34.944170611673336 17.865784099354663 56-57 14.284538567798316 35.33820972890297 32.78626877237142 17.590982930927296 58-59 14.514627954238973 33.88443251388089 33.46050357389317 18.14043595798697 60-61 14.405451128771649 33.2292442269801 34.90961216044411 17.455692483804143 62-63 16.319156048052186 32.73858963191054 34.31745436865668 16.624799951380588 64-65 15.319655371058513 33.79743870555242 35.053270619813 15.829635303576065 66-67 14.684976462812763 32.86090142742468 35.038122899821985 17.41599920994057 68-69 14.345655534608476 33.825005919062676 34.924115717358745 16.90522282897011 70-71 14.70479104324514 33.20969377998914 33.73181656432317 18.353698612442553 72-73 14.23245589041651 31.681020563999894 34.49114262594642 19.59538091963717 74-75 14.018351923330194 32.90809348691609 34.59448986414613 18.479064725607593 76-77 14.662829009849085 30.846327423206766 35.96612968173449 18.524713885209657 78-79 13.979545586905934 30.71061234101492 36.89036201627136 18.41948005580778 80-81 13.717183891747355 32.189285135518375 35.62636733784386 18.46716363489041 82-83 13.33472599401156 31.26814415304075 35.057320647460614 20.339809205487082 84-85 13.659779783480342 30.41707443986838 34.96522797106825 20.95791780558302 86-87 14.707141048366676 29.973157761458598 35.25386173714865 20.065839453026083 88-89 15.322431528101784 28.646367626906994 35.52813217932253 20.503068665668685 90-91 14.424588123102719 29.01241994536592 35.872707907757814 20.69028402377355 92-93 14.150340081781723 30.31027159687107 35.041277101751 20.498111219596208 94-95 13.966001395814837 30.078796407360077 34.42925486033619 21.525947336488905 96-97 13.968789935571142 28.86734646059226 33.88157603099475 23.28228757284185 98-99 15.47769797291137 28.409631963368554 33.10025022033572 23.012419843384357 100-101 16.2344120378406 27.33358587426249 33.984576421714635 22.447425666182276 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8085.0 1 5201.0 2 3746.5 3 4817.5 4 3607.0 5 3263.0 6 5476.0 7 10343.0 8 14667.0 9 14114.0 10 9622.5 11 5904.0 12 4853.5 13 4631.0 14 4229.0 15 3493.5 16 2844.5 17 2461.5 18 2243.0 19 2173.0 20 2076.5 21 2009.5 22 2069.5 23 2243.0 24 2383.5 25 2580.5 26 2813.5 27 3033.0 28 3322.0 29 3623.0 30 3981.5 31 4421.0 32 5088.5 33 5803.0 34 6246.5 35 6601.0 36 7280.0 37 7965.0 38 8285.0 39 8292.0 40 7979.0 41 7630.0 42 7167.5 43 6739.0 44 6699.5 45 6964.5 46 8423.0 47 12473.0 48 24182.5 49 26278.0 50 17576.5 51 12562.5 52 7653.5 53 5348.5 54 4477.5 55 3989.0 56 3690.0 57 3403.5 58 3170.0 59 2951.0 60 2657.0 61 2374.5 62 2057.0 63 1511.0 64 1003.5 65 778.0 66 643.0 67 475.0 68 333.5 69 296.0 70 294.0 71 226.0 72 183.0 73 202.0 74 179.5 75 117.5 76 88.0 77 75.5 78 57.5 79 54.0 80 47.0 81 29.5 82 15.5 83 10.0 84 10.0 85 8.5 86 6.5 87 4.5 88 2.0 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008355488036460312 2 0.10532978858083303 3 0.11950879858209901 4 0.12457273072540827 5 0.15951386251424232 6 0.09418913786555261 7 0.022534498037726294 8 0.023800481073553616 9 0.01949613875174073 10-11 0.05355108241549563 12-13 0.0538042790226611 14-15 0.02468666919863274 16-17 0.03240916571717939 18-19 0.038612482592733255 20-21 0.06026079250538043 22-23 0.03380174705658944 24-25 0.0502595265223446 26-27 0.058994809469553114 28-29 0.055576655272819346 30-31 0.07165463982782631 32-33 0.05228509937966831 34-35 0.06595771616660337 36-37 0.05671604000506394 38-39 0.029624003038359284 40-41 0.037599696164071404 42-43 0.03240916571717939 44-45 0.006583111786302064 46-47 0.012913026965438664 48-49 0.007975693125712115 50-51 0.008608684643625776 52-53 0.008482086340043044 54-55 0.010507659197366756 56-57 0.013799215090517788 58-59 0.01835675401949614 60-61 0.024306874287884542 62-63 0.011647043929611344 64-65 0.00822888973287758 66-67 0.010887454108114952 68-69 0.010001265983035827 70-71 0.030256994556272943 72-73 0.027725028484618303 74-75 0.02886441321686289 76-77 0.023040891252057223 78-79 0.005823521964805672 80-81 0.0055703253576402075 82-83 0.00569692366122294 84-85 0.005190530446892012 86-87 0.0126598303582732 88-89 0.03873908089631599 90-91 0.03582731991391316 92-93 0.03000379794910748 94-95 0.0502595265223446 96-97 0.043929611343208004 98-99 0.024813267502215467 100-101 0.07456640081022914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 394950.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.657250703997015 #Duplication Level Percentage of deduplicated Percentage of total 1 78.05573943035644 27.051973303237286 2 10.671851608262713 7.397140733268293 3 4.11159441211379 4.274896750013426 4 2.0279492081560124 2.8113257648814063 5 1.2570213463774675 2.1782451970839887 6 0.8090487494266173 1.6823643204380123 7 0.5274713155990947 1.279649393671947 8 0.4142719381889358 1.1486021137155764 9 0.26910568796685025 0.8393816964364851 >10 1.5934162615251484 9.902798987966333 >50 0.12105551937909363 2.826622252985492 >100 0.10501201681078001 7.796210357460969 >500 0.016772752685055143 4.176748869173663 >1k 0.016043502568313613 12.426118534539048 >5k 7.292501167415279E-4 1.3511375683530438 >10k+ 0.0029170004669661115 12.856784156775033 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17399 4.405367768071907 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12077 3.0578554247373084 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10950 2.772502848461831 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10444 2.6443853652361056 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5346 1.3535890619065705 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4632 1.1728066843904292 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4342 1.0993796683124446 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3523 0.8920116470439295 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3407 0.8626408406127358 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3365 0.8520065831117862 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3013 0.762881377389543 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2644 0.6694518293454867 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2256 0.5712115457652868 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2022 0.5119635396885681 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1974 0.49981010254462593 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1816 0.45980503861248256 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1815 0.45955184200531707 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1789 0.45296873021901507 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1786 0.45220914039751864 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1619 0.4099253070008862 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1533 0.3881503987846563 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1502 0.38030130396252687 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1490 0.3772629446765413 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1203 0.30459551842005317 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1200 0.3038359285985568 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1132 0.28661855931130525 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1103 0.27927585770350677 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 911 0.23066210912773769 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 909 0.23015571591340675 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 894 0.2263577668059248 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 867 0.21952145841245724 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 847 0.214457526269148 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 834 0.21116597037599696 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 813 0.20584884162552225 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 801 0.20281048233953664 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 743 0.18812507912393975 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 723 0.18306114698063045 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 701 0.17749082162299024 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 693 0.17546524876566655 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 691 0.1749588555513356 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 662 0.16761615394353716 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 655 0.1658437776933789 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 641 0.1622990251930624 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 640 0.16204582858589694 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 612 0.15495632358526396 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 606 0.15343714394227118 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 597 0.15115837447778202 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 593 0.15014558804912015 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 568 0.14381567286998354 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 525 0.1329282187618686 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 497 0.1258387137612356 No Hit ACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAA 495 0.12533232054690466 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACAAAAAAAAAAAAAAAA 487 0.12330674768958096 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 481 0.12178756804658818 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 474 0.12001519179642992 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 465 0.11773642233194075 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 463 0.11723002911760982 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 458 0.11596404608178251 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 454 0.11495125965312064 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 429 0.10862134447398406 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 415 0.10507659197366755 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 414 0.10482339536650208 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 409 0.10355741233067477 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 398 0.10077224965185468 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.53196607165464E-4 0.0 0.0 0.0 0.0 7 5.06393214330928E-4 0.0 0.0 0.0 0.0 8 5.06393214330928E-4 0.0 0.0 0.0 0.0 9 5.06393214330928E-4 0.0 0.0 0.0 0.0 10-11 5.06393214330928E-4 0.0 0.0 1.26598303582732E-4 0.0 12-13 5.06393214330928E-4 0.0 0.0 2.53196607165464E-4 0.0 14-15 6.329915179136599E-4 0.0 0.0 2.53196607165464E-4 0.0 16-17 7.595898214963919E-4 0.0 0.0 2.53196607165464E-4 0.0 18-19 7.595898214963919E-4 0.0 0.0 6.329915179136599E-4 0.0 20-21 7.595898214963919E-4 0.0 0.0 0.0024053677680719076 0.0 22-23 0.001012786428661856 0.0 0.0 0.0063299151791366 0.0 24-25 0.001012786428661856 0.0 0.0 0.01658437776933789 0.0 26-27 0.001012786428661856 0.0 0.0 0.03392834536017217 0.0 28-29 0.001012786428661856 0.0 0.0 0.06823648563109254 0.0 30-31 0.001012786428661856 0.0 0.0 0.1515381693885302 0.0 32-33 0.001012786428661856 0.0 0.0 0.27902266109634133 0.0 34-35 0.001012786428661856 0.0 0.0 0.4396759083428282 0.0 36-37 0.001012786428661856 0.0 0.0 0.6567919989872135 0.0 38-39 0.0012659830358273199 0.0 0.0 0.9497404734776553 0.0 40-41 0.0012659830358273199 0.0 0.0 1.2444613242182556 0.0 42-43 0.0015191796429927839 0.0 0.0 1.5019622737055323 0.0 44-45 0.002025572857323712 0.0 0.0 1.765666540068363 0.0 46-47 0.002025572857323712 0.0 0.0 2.0759589821496394 0.0 48-49 0.002025572857323712 0.0 0.0 2.41334346119762 0.0 50-51 0.002025572857323712 0.0 0.0 2.7199645524749965 0.0 52-53 0.002152171160906444 0.0 0.0 3.002405367768072 0.0 54-55 0.002278769464489176 0.0 0.0 3.273325737435118 0.0 56-57 0.0027851626788201037 0.0 0.0 3.574249905051272 0.0 58-59 0.0027851626788201037 0.0 0.0 3.9380934295480436 0.0 60-61 0.0027851626788201037 0.0 0.0 4.392454741106469 0.0 62-63 0.0027851626788201037 0.0 0.0 4.783770097480694 0.0 64-65 0.0027851626788201037 0.0 0.0 5.085833649829093 0.0 66-67 0.0027851626788201037 0.0 0.0 5.420559564501835 0.0 68-69 0.0027851626788201037 0.0 0.0 5.804532219268262 0.0 70-71 0.0027851626788201037 0.0 0.0 6.268388403595392 0.0 72-73 0.0027851626788201037 0.0 0.0 6.71781238131409 0.0 74-75 0.0027851626788201037 0.0 0.0 7.153310545638688 0.0 76-77 0.0027851626788201037 0.0 0.0 7.524496771743259 0.0 78-79 0.0027851626788201037 0.0 0.0 7.9672110393720725 0.0 80-81 0.0027851626788201037 0.0 0.0 8.423218128877073 0.0 82-83 0.0027851626788201037 0.0 0.0 8.86973034561337 0.0 84-85 0.0027851626788201037 0.0 0.0 9.450183567540195 0.0 86-87 0.0027851626788201037 0.0 0.0 10.044815799468289 0.0 88-89 0.0027851626788201037 0.0 0.0 10.523483985314597 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATATA 50 2.8003988E-5 47.56633 1 GTATAGG 45 9.5515494E-4 42.28118 1 GGATAGA 50 0.0016034982 38.053062 1 CATACAG 50 0.0016044978 38.048237 5 ACACGTC 25 0.001615499 38.00005 82-83 GTAAGCG 70 1.0913936E-11 37.32384 94-95 AGGCTAT 55 0.002572766 34.55863 6 GGGAATG 115 1.0277108E-7 33.06446 7 ACATGGC 135 1.1783413E-8 31.706865 3 ATGGGCG 90 2.3985483E-5 31.686773 7 CAATGGG 60 0.0039355867 31.686773 9 GGGATAG 60 0.0039355867 31.686773 7 GAATATC 30 0.0039501837 31.666708 30-31 CATGGGT 440 0.0 30.265644 4 GGGAACT 195 1.6370905E-11 29.24933 7 GGGAAGT 280 0.0 28.8576 7 ATGGGGT 320 0.0 28.221033 7 CATGGCC 85 6.3523627E-4 27.976645 4 GGGATTG 255 0.0 27.95892 7 TAACTGC 85 6.3854153E-4 27.951834 6 >>END_MODULE