FastQCFastQC Report
Thu 26 May 2016
SRR938523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938523_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394950
Sequences flagged as poor quality0
Sequence length101
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162074.103557412330675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127223.2211672363590327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114382.896062792758577No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA112102.8383339663248512No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA52891.339156855298139No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA44131.1173566274211926No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43031.0895050006329916No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38150.9659450563362451No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35620.9018863147233828No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30960.7838966957842765No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30830.7806051398911255No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25920.6562856057728826No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23870.6043803013039626No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG22450.5684263830864666No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20770.5258893530826687No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA19400.49120141790100014No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18750.474743638435245No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT18360.46486897075579187No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17630.446385618432713No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17520.44360045575389284No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC15830.4008102291429295No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14080.3565008228889733No Hit
GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA13890.3516900873528295No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12230.30965945056336247No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG12010.30408912520572223No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG11090.2807950373464996No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10490.2656032409165717No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT9170.23218128877073047No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT8940.2263577668059248No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG8880.22483858716293204No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT8800.22281301430560832No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8610.21800227876946449No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA8610.21800227876946449No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG8530.21597670591214077No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8520.21572350930497533No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG8350.21141916698316243No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT7780.19698696037473098No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7680.19445499430307633No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG7400.18736548930244334No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT7380.18685909608811244No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7380.18685909608811244No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.17673123180149386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6910.1749588555513356No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.1693885301936954No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA6660.16862894037219903No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA6610.1673629573363717No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.16356500822888972No Hit
GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA6370.16128623876440054No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6290.15926066590707685No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6080.1539435371566021No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG5810.14710722876313456No Hit
ACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAA5640.1428028864413217No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5620.14229649322699076No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC5290.13394100519053045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5160.13064944929737943No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT4550.11520445626028611No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC4490.11368527661729333No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC4450.11267249018863147No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA4450.11267249018863147No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT4440.11241929358146602No Hit
GTACATGGGACTCCCGACCCGGGGAGGTAGTGACAAAAAAAAAAAAAAAA4420.11191290036713507No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4350.11014052411697683No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC4330.1096341309026459No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4280.10836814786681857No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA4260.10786175465248767No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4220.10684896822382581No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT4040.10229142929484746No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4040.10229142929484746No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.10153183947335105No Hit
ACGCAGAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAA3990.10102544625902013No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT3950.10001265983035827TruSeq Adapter, Index 10 (95% over 21bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG3950.10001265983035827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTC452.0498192E-763.308187
AGATAGT656.712071E-1058.4383246
TAGATAG652.5830905E-643.850955
TGGGACG554.9690025E-543.164676
GTTCGAC402.2684617E-741.54599416-17
TAGTCAA802.2646054E-741.5459949
ATGGGCG608.2755236E-539.587665
CATATAG608.2755236E-539.587663
TCGACCG308.307816E-539.5676118-19
GGGATAG853.643945E-739.1021167
AGTATCC500.001617685337.984918
AAGTATC550.002582609734.5317347
AGTCTGG550.002582609734.5317346
TACCTGG1404.5110937E-1033.945182
GTATATG851.62405E-533.5458261
ATAGTCA1001.300361E-633.2367948
GGACCCG1151.0370786E-733.0303579
ACCTGGG1456.6029315E-1032.7622033
TACTAGT600.00393838931.682172
GGATAAG902.4009334E-531.6821691
Produced by FastQC (version 0.11.5)