##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938523_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 394950 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.623078870743132 31.0 31.0 34.0 30.0 34.0 2 31.690322825674137 31.0 31.0 34.0 30.0 34.0 3 31.663175085453855 31.0 31.0 34.0 30.0 34.0 4 35.41473604253703 37.0 35.0 37.0 33.0 37.0 5 35.39660969743005 37.0 35.0 37.0 33.0 37.0 6 35.508996075452586 37.0 35.0 37.0 33.0 37.0 7 35.44071907836435 37.0 35.0 37.0 33.0 37.0 8 35.50343840992531 37.0 35.0 37.0 33.0 37.0 9 36.913915685529815 39.0 37.0 39.0 32.0 39.0 10-11 36.837067983289025 39.0 37.0 39.0 32.0 39.0 12-13 36.67878339030257 39.0 36.0 39.0 32.0 39.0 14-15 37.189922775034816 39.0 36.0 41.0 32.0 41.0 16-17 37.25719837954171 39.0 36.0 40.0 31.5 41.0 18-19 37.75562602861122 39.5 37.0 41.0 33.0 41.0 20-21 37.84187112292695 40.0 37.0 41.0 33.0 41.0 22-23 37.643721990125336 39.5 36.0 41.0 32.5 41.0 24-25 37.38947968097227 39.0 35.5 41.0 32.0 41.0 26-27 36.921590074693 39.0 35.0 41.0 31.5 41.0 28-29 36.57694011900241 38.5 35.0 40.5 31.0 41.0 30-31 35.96699075832384 37.5 35.0 40.0 30.0 41.0 32-33 35.28265096847702 35.5 34.5 40.0 30.0 41.0 34-35 34.70653627041398 35.0 33.0 40.0 27.5 41.0 36-37 34.299944296746425 35.0 33.0 40.0 25.0 41.0 38-39 33.855129763261175 35.0 33.0 40.0 24.0 41.0 40-41 33.41494619572098 35.0 33.0 39.0 22.0 41.0 42-43 32.92085200658311 35.0 33.0 39.0 20.5 41.0 44-45 32.156828712495255 35.0 33.0 39.0 14.0 41.0 46-47 31.132783896695784 35.0 31.0 39.0 2.0 41.0 48-49 30.323419420179768 35.0 29.5 39.0 2.0 41.0 50-51 29.87087606026079 35.0 28.0 38.0 2.0 40.0 52-53 29.314422078744144 35.0 27.0 38.0 2.0 40.0 54-55 29.030941891378657 35.0 27.0 38.0 2.0 40.0 56-57 28.71677807317382 35.0 26.0 38.0 2.0 40.0 58-59 28.283806810988736 34.5 25.0 38.0 2.0 40.0 60-61 27.78638941638182 34.0 24.0 37.0 2.0 40.0 62-63 27.20616280541841 33.0 22.0 36.0 2.0 39.0 64-65 26.591039372072416 33.0 21.0 36.0 2.0 39.0 66-67 26.040559564501834 33.0 19.0 35.0 2.0 39.0 68-69 25.671083681478667 33.0 17.5 35.0 2.0 38.0 70-71 25.248250411444488 33.0 11.5 35.0 2.0 37.5 72-73 24.79211165970376 32.5 7.0 35.0 2.0 37.0 74-75 24.261041904038485 32.0 2.0 35.0 2.0 36.0 76-77 23.03395872895303 30.0 2.0 33.5 2.0 35.0 78-79 23.615236105836182 31.0 2.0 35.0 2.0 35.0 80-81 23.292738321306494 31.0 2.0 35.0 2.0 35.0 82-83 22.92388276997088 31.0 2.0 35.0 2.0 35.0 84-85 22.45092290163312 31.0 2.0 34.5 2.0 35.0 86-87 21.905536143815674 30.5 2.0 34.0 2.0 35.0 88-89 21.152999113811873 29.0 2.0 34.0 2.0 35.0 90-91 20.56258513735916 29.0 2.0 34.0 2.0 35.0 92-93 19.877817445246237 27.0 2.0 34.0 2.0 35.0 94-95 19.239978478288393 26.5 2.0 33.0 2.0 35.0 96-97 18.355061400177238 24.5 2.0 33.0 2.0 35.0 98-99 17.384780351943284 21.5 2.0 33.0 2.0 35.0 100-101 14.53452968730219 2.0 2.0 29.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39065157408023055 1101 2 0.5402141819278192 1101 3 0.348356354536314 1101 4 0.3286643109303782 1101 5 0.09346062698635649 1101 6 0.19183957788424522 1101 7 0.3706453380160255 1101 8 0.4427208716645197 1101 9 0.7355464489781198 1101 10-11 0.7156532905021038 1101 12-13 0.6966122998170761 1101 14-15 1.131288492802362 1101 16-17 0.7090150085726137 1101 18-19 0.5517062716676762 1101 20-21 0.6928325376164324 1101 22-23 0.6558740865057331 1101 24-25 0.557002264744078 1101 26-27 0.6776481099345162 1101 28-29 0.6263955971341346 1101 30-31 0.887317514003449 1101 32-33 0.8033740179561377 1101 34-35 0.7357284774306478 1101 36-37 0.992194823764919 1101 38-39 1.124472222166915 1101 40-41 1.2816315860016232 1101 42-43 1.2914049384210529 1101 44-45 1.0598090515096388 1101 46-47 1.1096967098936688 1101 48-49 1.255456876410463 1101 50-51 1.0211704778493527 1101 52-53 1.5226462931352387 1101 54-55 1.2986967236815197 1101 56-57 0.8881607109617491 1101 58-59 1.1965713501070638 1101 60-61 1.2155417770422439 1101 62-63 1.1038952814987582 1101 64-65 0.8798579698534326 1101 66-67 1.0350024820537378 1101 68-69 1.1820917311208383 1101 70-71 1.0380125737447266 1101 72-73 0.9962514023372151 1101 74-75 0.7510321947537868 1101 76-77 0.7562317429773415 1101 78-79 1.1108531444772503 1101 80-81 0.6909514238312262 1101 82-83 0.526802705604446 1101 84-85 0.6516516857058008 1101 86-87 0.7133221845962048 1101 88-89 0.6416256549540194 1101 90-91 0.2868636861480951 1101 92-93 0.4397096529312421 1101 94-95 0.26778423676082497 1101 96-97 0.5432511293171345 1101 98-99 1.2205825232807364 1101 100-101 1.5002028306792141 1102 1 0.3205718701649758 1102 2 0.26027421485850155 1102 3 0.24901788246182832 1102 4 0.15693661564121442 1102 5 -0.009608046606437881 1102 6 0.24779260065887598 1102 7 -0.021353671149199727 1102 8 0.07203209578260328 1102 9 -0.05240033736584593 1102 10-11 0.2358451918848985 1102 12-13 0.37010674094254625 1102 14-15 0.4234754691534661 1102 16-17 0.3592576173823048 1102 18-19 0.2914737169167694 1102 20-21 0.22452103664745238 1102 22-23 0.244266019944952 1102 24-25 0.4059072466030571 1102 26-27 0.34833397159649593 1102 28-29 0.4479834098663602 1102 30-31 0.4440683044856044 1102 32-33 0.43757020324223816 1102 34-35 0.5673557778430123 1102 36-37 1.0515684121896385 1102 38-39 0.8498324926065237 1102 40-41 0.7648930352432046 1102 42-43 0.7375902245245953 1102 44-45 0.920407340862532 1102 46-47 0.7761132393491472 1102 48-49 0.5938160248754016 1102 50-51 0.8291306696050249 1102 52-53 0.7661694393270828 1102 54-55 0.9208681089559683 1102 56-57 1.2147172686851597 1102 58-59 1.0666117120531808 1102 60-61 1.1089833250758758 1102 62-63 1.0729462657765403 1102 64-65 0.9791454430790338 1102 66-67 1.1684482557062772 1102 68-69 1.7743413632005698 1102 70-71 1.7769786085113708 1102 72-73 1.7512857172769607 1102 74-75 1.4674312388896489 1102 76-77 1.287024119377989 1102 78-79 1.3415828651492738 1102 80-81 1.5374376031431787 1102 82-83 1.547876158517937 1102 84-85 1.603902192051514 1102 86-87 1.4670742427640349 1102 88-89 1.0729775615824124 1102 90-91 1.077546196457149 1102 92-93 1.6923615930731621 1102 94-95 1.5193615583012843 1102 96-97 1.549669698726568 1102 98-99 1.7168231795630646 1102 100-101 2.0601337636667267 1103 1 0.3710954887032969 1103 2 0.31072820327205264 1103 3 0.4463496560803648 1103 4 0.34248697214744794 1103 5 0.24550280455220275 1103 6 0.17277310422393555 1103 7 0.25184708642655806 1103 8 0.3773044888307311 1103 9 0.32045505479101877 1103 10-11 0.25318309295442276 1103 12-13 0.3688882137589502 1103 14-15 0.5404123007042685 1103 16-17 0.6294921316425572 1103 18-19 0.5474062035477871 1103 20-21 0.25123115251198413 1103 22-23 0.3709023278772108 1103 24-25 0.46319195025724724 1103 26-27 0.6025814092079074 1103 28-29 0.5555828416845401 1103 30-31 0.7629951650025362 1103 32-33 1.0729868699089096 1103 34-35 1.17340942089114 1103 36-37 1.229731012457016 1103 38-39 1.4689069689879872 1103 40-41 1.052493302622878 1103 42-43 0.7688402245245953 1103 44-45 0.8379652731263505 1103 46-47 0.9144125931815879 1103 48-49 1.0423941777274521 1103 50-51 1.3457653064142967 1103 52-53 1.3640972468137136 1103 54-55 1.0653506178686207 1103 56-57 1.3097554259935364 1103 58-59 1.4927828907519327 1103 60-61 1.5166955576961954 1103 62-63 1.1331832867212839 1103 64-65 1.0955461226690524 1103 66-67 0.9336067338702669 1103 68-69 1.2261851689046672 1103 70-71 0.42690619318160117 1103 72-73 0.6000490862609205 1103 74-75 0.36779749334597867 1103 76-77 0.2541029963833381 1103 78-79 0.5675047680013243 1103 80-81 0.7696192542126958 1103 82-83 0.560465285077651 1103 84-85 0.6670567152244189 1103 86-87 0.9928443519440684 1103 88-89 0.9612072852900795 1103 90-91 1.0244601893270264 1103 92-93 0.9251490262282438 1103 94-95 0.8875865471604598 1103 96-97 1.3921385044306653 1103 98-99 1.8502693136985364 1103 100-101 1.8251808336310766 1104 1 0.30303471176094376 1104 2 0.3428201518936014 1104 3 0.35412691171946165 1104 4 0.33144373906474556 1104 5 0.280884226857971 1104 6 0.3091492007151686 1104 7 0.31391632201552966 1104 8 0.25138421940717137 1104 9 0.39858005479101877 1104 10-11 0.43106921450830527 1104 12-13 0.5125383547363924 1104 14-15 0.5907921565939915 1104 16-17 0.5758619624696237 1104 18-19 0.38344160455029197 1104 20-21 0.340790989604713 1104 22-23 0.48253884103511524 1104 24-25 0.3087726990041162 1104 26-27 0.2996561272530158 1104 28-29 0.6216562282760236 1104 30-31 0.4545188962055491 1104 32-33 0.36175566690138794 1104 34-35 0.7523469992119445 1104 36-37 0.5040398313793233 1104 38-39 0.498482861845126 1104 40-41 0.6066129768083428 1104 42-43 0.3509987458278516 1104 44-45 0.030321162850661665 1104 46-47 0.08881343904625183 1104 48-49 0.30718701920614677 1104 50-51 0.5865724022789571 1104 52-53 0.6765972468137136 1104 54-55 0.8248861755127344 1104 56-57 0.6150597023093276 1104 58-59 0.7793293349875228 1104 60-61 0.8217746617062218 1104 62-63 1.1946944865959672 1104 64-65 0.9603350481076518 1104 66-67 0.6021628898853066 1104 68-69 0.5669981388294794 1104 70-71 0.6878319450613013 1104 72-73 0.6198642491681916 1104 74-75 0.8643024275565026 1104 76-77 0.6533041242028865 1104 78-79 0.9409187717607175 1104 80-81 0.48516789143074135 1104 82-83 0.8419502474836662 1104 84-85 1.0378078117156484 1104 86-87 1.322310595678406 1104 88-89 0.9638310321572447 1104 90-91 1.247439512635296 1104 92-93 1.1290180738472912 1104 94-95 1.1791259425238678 1104 96-97 1.5177650708467105 1104 98-99 1.549801346906559 1104 100-101 1.535560297916792 1105 1 0.009668721026532268 1105 2 0.007303329534675385 1105 3 0.07908324193895311 1105 4 0.029040002450479108 1105 5 -0.042139240902336894 1105 6 0.04027626078959656 1105 7 0.17309077329525024 1105 8 0.10560309994184536 1105 9 0.3884790446900084 1105 10-11 0.2046508959847202 1105 12-13 0.2917496405266249 1105 14-15 0.379506366360836 1105 16-17 0.12893973265265402 1105 18-19 0.09175595102253453 1105 20-21 -0.01470950405367688 1105 22-23 0.10129216373579908 1105 24-25 0.13897320025724724 1105 26-27 0.05941537385437101 1105 28-29 0.22576118764413877 1105 30-31 0.5072738897500173 1105 32-33 0.46282272849476414 1105 34-35 0.4127954688709323 1105 36-37 0.04250721195196405 1105 38-39 -0.10397024060797833 1105 40-41 0.18222815110772927 1105 42-43 0.2969336588680278 1105 44-45 0.18771274787381742 1105 46-47 0.14665691136340797 1105 48-49 0.5333585085355317 1105 50-51 0.5572946624748987 1105 52-53 0.6324053276217967 1105 54-55 0.46647119867670384 1105 56-57 -0.28758516744080254 1105 58-59 0.12729204479234113 1105 60-61 0.0022542698174063958 1105 62-63 -0.008901624894878779 1105 64-65 -0.24879573339155314 1105 66-67 -0.29189042522063957 1105 68-69 -0.023341346246844807 1105 70-71 0.014500890151300183 1105 72-73 0.22915262161445682 1105 74-75 0.009172177689411143 1105 76-77 0.02335804688839005 1105 78-79 0.16622635891041426 1105 80-81 0.21205138805107993 1105 82-83 0.0686723557847202 1105 84-85 -0.039145936290733374 1105 86-87 0.007404011034982005 1105 88-89 0.1820090529668441 1105 90-91 0.1226293812462167 1105 92-93 0.39831821814743407 1105 94-95 0.6448071910998578 1105 96-97 0.41107789837006337 1105 98-99 0.36766988188035654 1105 100-101 0.2379412502977445 1106 1 0.07423380924890921 1106 2 0.19540557965738614 1106 3 0.16543266972060522 1106 4 0.08079153310226417 1106 5 -0.03739228283057372 1106 6 0.1933866485547142 1106 7 0.11686999776500784 1106 8 0.29756104253917215 1106 9 0.2468323992070438 1106 10-11 0.1541781377114546 1106 12-13 -2.1411467242415938E-4 1106 14-15 0.20515414853035452 1106 16-17 0.1467488984797214 1106 18-19 0.24485665111811983 1106 20-21 0.5592687697327818 1106 22-23 0.7789672522165176 1106 24-25 0.65666288482673 1106 26-27 0.6648882612965679 1106 28-29 0.6419499292804431 1106 30-31 0.4755144797936737 1106 32-33 0.11979526718085509 1106 34-35 0.024981911300343995 1106 36-37 -0.041258837287230676 1106 38-39 -0.12093012073921017 1106 40-41 -0.045093014512673335 1106 42-43 -0.18248650181811854 1106 44-45 -0.5727337891072395 1106 46-47 -1.26178492386871 1106 48-49 -0.9063583531762163 1106 50-51 -0.6915621925200632 1106 52-53 -0.8643234692988173 1106 54-55 -0.5348398659335949 1106 56-57 -0.4276333474506231 1106 58-59 -0.7749367650451688 1106 60-61 -0.3975267888511169 1106 62-63 -0.9314713115310624 1106 64-65 -0.6624495348330797 1106 66-67 -0.7885966175790067 1106 68-69 -0.8756237611891073 1106 70-71 -0.9294850046018581 1106 72-73 -0.278067921411715 1106 74-75 -0.6817782472763589 1106 76-77 -0.6051521187061759 1106 78-79 -0.28263243361845625 1106 80-81 -1.4502396812093004 1106 82-83 -1.2115402562693234 1106 84-85 -0.9479462152956124 1106 86-87 -0.5251949969476577 1106 88-89 -0.06414171300489357 1106 90-91 -0.0559692650634247 1106 92-93 0.27705120014129037 1106 94-95 0.5237791356088835 1106 96-97 0.5442058531092719 1106 98-99 0.8596969298536976 1106 100-101 0.7091688717811202 1107 1 0.16455291404631822 1107 2 0.1282158445149868 1107 3 0.15464515648391952 1107 4 -0.011150033502268286 1107 5 0.049118371541709394 1107 6 0.25154756951852875 1107 7 0.13129876116425265 1107 8 0.23783339318908503 1107 9 0.2298956318693044 1107 10-11 0.07413875276071735 1107 12-13 0.13445331162012053 1107 14-15 0.06929774259288735 1107 16-17 0.05829686328096528 1107 18-19 0.30644827376570305 1107 20-21 0.13287713717702587 1107 22-23 0.11460629781264942 1107 24-25 0.08590621074958449 1107 26-27 0.15429271873171047 1107 28-29 0.19699287497752493 1107 30-31 -0.015193520429257035 1107 32-33 -0.33301091055921717 1107 34-35 -0.3848816656706049 1107 36-37 -0.37187877870520936 1107 38-39 -0.4232281954591528 1107 40-41 -0.4686174721955254 1107 42-43 -0.8477608650637833 1107 44-45 -0.9136489318776881 1107 46-47 -1.011012396729626 1107 48-49 -1.5938579661385717 1107 50-51 -1.310670705490466 1107 52-53 -1.0594802350264807 1107 54-55 -1.2295507642944123 1107 56-57 -1.3786746335302702 1107 58-59 -1.2203520719195744 1107 60-61 -1.2985473297130063 1107 62-63 -1.3862281590010745 1107 64-65 -1.6059110756763886 1107 66-67 -1.4744056501087464 1107 68-69 -1.0126288948564302 1107 70-71 -0.9449788955996716 1107 72-73 -1.7698813011482812 1107 74-75 -1.6241220234014015 1107 76-77 -1.2813842917522535 1107 78-79 -1.1160669127896377 1107 80-81 -0.8887966810173289 1107 82-83 -0.9562316479328423 1107 84-85 -0.894692931668235 1107 86-87 -0.687905622833977 1107 88-89 -0.5624635177769122 1107 90-91 -0.5068402545793518 1107 92-93 -0.5033467364836213 1107 94-95 -0.33075044335608084 1107 96-97 -0.43705842857982447 1107 98-99 -0.40037069336360886 1107 100-101 0.02426086288854279 1108 1 0.24576439660875948 1108 2 0.18279294451084382 1108 3 0.06738928525722088 1108 4 0.11018766489398502 1108 5 0.24190536160192266 1108 6 0.34723953657927353 1108 7 0.19058231462541642 1108 8 0.19375849249821187 1108 9 0.20950100018628603 1108 10-11 0.20541032400576853 1108 12-13 0.12771627508739414 1108 14-15 0.16103806476294835 1108 16-17 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-0.28869782670233235 2316 76-77 -0.17328226931714497 2316 78-79 -0.456687562916553 2316 80-81 -0.8644198460288486 2316 82-83 -0.8812617680624442 2316 84-85 -1.195601799268335 2316 86-87 -1.5798269946742884 2316 88-89 -1.484218439347604 2316 90-91 -1.2595072019773212 2316 92-93 -1.3956541138683747 2316 94-95 -1.2412819612936445 2316 96-97 -0.5142624617529066 2316 98-99 -1.2325167672193373 2316 100-101 -0.7415273487360707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 29.0 5 115.0 6 226.0 7 467.0 8 778.0 9 1196.0 10 2003.0 11 2852.0 12 3733.0 13 5296.0 14 6163.0 15 8116.0 16 10286.0 17 12676.0 18 10593.0 19 6913.0 20 6105.0 21 6373.0 22 7097.0 23 7571.0 24 8138.0 25 8498.0 26 9270.0 27 10310.0 28 11738.0 29 13280.0 30 15014.0 31 17357.0 32 20436.0 33 24703.0 34 34332.0 35 47908.0 36 33525.0 37 29752.0 38 11696.0 39 398.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.659577161666036 19.439169515128498 15.252816812254716 12.648436510950752 2 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14.83592859855678 20-21 17.43562476262818 27.872768704899354 39.39511330548171 15.296493226990759 22-23 15.850740600075957 29.41372325610837 38.945182934548676 15.790353209266996 24-25 16.20997831242016 30.336910792261346 39.75182723873936 13.701283656579136 26-27 16.331406043679884 28.736660999160634 41.583753543246274 13.3481794139132 28-29 14.007993427007756 30.767351268959704 42.06740355412528 13.157251749907267 30-31 14.005079175380938 32.72156193504935 40.54820152628461 12.725157363285108 32-33 13.402616540363441 32.80208330695862 39.93933393931875 13.855966213359194 34-35 13.975860292041595 31.653328167592825 39.90521879063431 14.465592749731268 36-37 14.8656810915192 31.99455506663712 40.37215486403495 12.767608977808731 38-39 15.441196950399682 31.548955909423526 40.32744463040941 12.68240250976738 40-41 13.212329296067432 33.16141087820978 40.68194219202396 12.944317633698827 42-43 12.54032196806652 34.567383895519896 38.86442703559474 14.027867100818847 44-45 13.23891734760799 34.231956426507715 37.22674776346542 15.302378462418876 46-47 13.566527408532725 34.50322825674136 35.72262311685023 16.20762121787568 48-49 14.245980503861247 34.0858336498291 35.44448664387897 16.223699202430687 50-51 14.168556153523815 35.27232031518033 35.343215634443474 15.215907896852373 52-53 13.271046967970628 35.750474743638435 34.52905431067224 16.4494239777187 54-55 12.163830199356621 34.822905945250675 34.841516107918686 18.17174774747402 56-57 14.343967590834284 35.28155462716799 32.46005823521965 17.914419546778074 58-59 14.482129855329386 33.97803032701256 33.19611257083162 18.34372724682643 60-61 14.41853399164451 33.48563109254336 34.34523357387011 17.75060134194202 62-63 16.127304633195834 33.28901955124066 33.753807819989824 16.829867995573686 64-65 15.211393509356366 34.20319252250302 34.42261988419604 16.162794083944576 66-67 14.709829511069247 33.34194260653342 34.202490105666165 17.74573777673116 68-69 14.44111431462003 34.23955915665412 33.96656448359563 17.352762045130223 70-71 14.89251804025826 33.69173313077604 32.684137232561085 18.73161159640461 72-73 14.400701861883608 32.23462746503034 33.276659421014585 20.088011252071468 74-75 14.208092426993666 33.37258745438291 33.318644694488974 19.100675424134447 76-77 14.535706278949881 31.138641513544457 35.42863873967557 18.89701346783009 78-79 13.86637894462237 31.06904189319079 36.44496112839723 18.61961803378961 80-81 13.616994872717003 32.56970711551392 35.26745068045086 18.545847331318214 82-83 13.218189923305268 31.83350556720667 34.68764880500086 20.2606557044872 84-85 13.615287411335395 30.935558780106714 34.36438778688587 21.08476602167202 86-87 14.414766871522952 30.7167944169699 34.714657226564974 20.153781484942176 88-89 15.063482470577355 29.544023424087733 34.54875562701372 20.84373847832119 90-91 14.244667905113214 29.68991983021379 35.387660361732756 20.677751902940233 92-93 13.84919484222909 31.269397673355527 34.193215072158395 20.688192412256985 94-95 13.707386813323186 30.895248901152478 33.51158871001442 21.885775575509914 96-97 13.582689131822374 30.108737500126647 32.94736122227738 23.3612121457736 98-99 14.854521296471043 29.586179554830483 32.196069142757985 23.36323000594049 100-101 15.289526696370803 28.973054828568134 33.15771680790692 22.57970166715414 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8140.0 1 5188.5 2 3614.5 3 4770.0 4 3739.5 5 3367.5 6 5536.0 7 10770.0 8 14677.5 9 13819.0 10 9706.0 11 5961.5 12 4910.5 13 4558.5 14 4000.5 15 3326.0 16 2746.0 17 2355.0 18 2184.5 19 2052.5 20 1966.5 21 1980.5 22 2027.5 23 2144.5 24 2268.5 25 2463.0 26 2729.0 27 2948.0 28 3209.0 29 3552.5 30 3942.5 31 4374.5 32 4982.0 33 5618.5 34 6117.5 35 6581.5 36 7374.5 37 8137.5 38 8363.0 39 8327.5 40 8219.0 41 7869.5 42 7251.5 43 6865.5 44 6881.5 45 6976.0 46 8080.5 47 12368.0 48 26307.5 49 27000.0 50 15770.5 51 12219.0 52 8557.5 53 6336.0 54 4630.0 55 3629.0 56 3434.5 57 3454.5 58 3359.5 59 3049.5 60 2576.0 61 2255.0 62 1884.5 63 1308.0 64 844.0 65 634.5 66 541.0 67 388.0 68 270.5 69 226.0 70 199.0 71 183.5 72 157.0 73 183.5 74 170.5 75 98.0 76 69.0 77 55.5 78 50.0 79 46.5 80 37.0 81 21.5 82 13.5 83 8.0 84 3.5 85 2.0 86 1.5 87 0.5 88 0.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.051652107861754656 3 0.0 4 0.0 5 0.04582858589694898 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005950120268388404 26-27 0.002152171160906444 28-29 1.26598303582732E-4 30-31 0.001012786428661856 32-33 2.53196607165464E-4 34-35 0.010254462590201291 36-37 0.0221547031269781 38-39 0.003291555893151032 40-41 0.001139384732244588 42-43 0.001012786428661856 44-45 0.007849094822129383 46-47 0.0 48-49 0.0 50-51 3.7979491074819596E-4 52-53 0.0 54-55 0.001139384732244588 56-57 0.0 58-59 0.0221547031269781 60-61 0.0 62-63 0.009874667679453095 64-65 0.014812001519179642 66-67 0.006836308393467527 68-69 0.01785036080516521 70-71 0.0 72-73 1.26598303582732E-4 74-75 0.022028104823395366 76-77 0.049246740093682746 78-79 0.031522977592100265 80-81 0.02569945562729459 82-83 0.03494113178883403 84-85 0.01595138625142423 86-87 0.009874667679453095 88-89 0.07937713634637296 90-91 0.025446259020129133 92-93 0.02266109634130903 94-95 0.026459045448790984 96-97 0.03696670464615774 98-99 0.051019116343840994 100-101 0.014052411697683252 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 394950.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.28483910923863 #Duplication Level Percentage of deduplicated Percentage of total 1 75.98596809020162 24.531947543519 2 11.557419696668921 7.462588708498033 3 4.527780519957857 4.385359968263528 4 2.2506122887577247 2.9064262255927384 5 1.3137826697285981 2.120763105834689 6 0.9183549270513668 1.7789364639017973 7 0.6238304920408829 1.4098186946883953 8 0.40722357113344326 1.0517717980426247 9 0.30992275011721504 0.9005225511444349 >10 1.8226459742957783 10.660028330445142 >50 0.12396812546489995 2.8038834757200313 >100 0.11533743318569804 7.8614620714582335 >500 0.021969034892513915 5.132051605211658 >1k 0.01726138455840379 12.58973824435462 >5k 7.846083890183541E-4 1.3397542418541193 >10k+ 0.0031384335560734164 13.064946971470961 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16207 4.103557412330675 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12722 3.2211672363590327 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11438 2.896062792758577 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11210 2.8383339663248512 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5289 1.339156855298139 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4413 1.1173566274211926 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4303 1.0895050006329916 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3815 0.9659450563362451 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3562 0.9018863147233828 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3096 0.7838966957842765 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3083 0.7806051398911255 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2592 0.6562856057728826 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2387 0.6043803013039626 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2245 0.5684263830864666 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2077 0.5258893530826687 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1940 0.49120141790100014 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1875 0.474743638435245 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1836 0.46486897075579187 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1763 0.446385618432713 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1752 0.44360045575389284 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1583 0.4008102291429295 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1408 0.3565008228889733 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1389 0.3516900873528295 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1223 0.30965945056336247 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1201 0.30408912520572223 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1109 0.2807950373464996 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1049 0.2656032409165717 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 917 0.23218128877073047 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 894 0.2263577668059248 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 888 0.22483858716293204 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 880 0.22281301430560832 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 861 0.21800227876946449 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 861 0.21800227876946449 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 853 0.21597670591214077 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 852 0.21572350930497533 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 835 0.21141916698316243 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 778 0.19698696037473098 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 768 0.19445499430307633 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 740 0.18736548930244334 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 738 0.18685909608811244 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 738 0.18685909608811244 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 0.17673123180149386 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 691 0.1749588555513356 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 669 0.1693885301936954 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 666 0.16862894037219903 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 661 0.1673629573363717 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 646 0.16356500822888972 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 637 0.16128623876440054 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 629 0.15926066590707685 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 608 0.1539435371566021 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 581 0.14710722876313456 No Hit ACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAA 564 0.1428028864413217 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 562 0.14229649322699076 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 529 0.13394100519053045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 516 0.13064944929737943 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 455 0.11520445626028611 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 449 0.11368527661729333 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 445 0.11267249018863147 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 445 0.11267249018863147 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 444 0.11241929358146602 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACAAAAAAAAAAAAAAAA 442 0.11191290036713507 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 435 0.11014052411697683 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 433 0.1096341309026459 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 428 0.10836814786681857 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 426 0.10786175465248767 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 422 0.10684896822382581 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 404 0.10229142929484746 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 404 0.10229142929484746 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 401 0.10153183947335105 No Hit ACGCAGAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAA 399 0.10102544625902013 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 395 0.10001265983035827 TruSeq Adapter, Index 10 (95% over 21bp) GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 395 0.10001265983035827 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.53196607165464E-4 0.0 0.0 0.0 0.0 7 2.53196607165464E-4 0.0 0.0 0.0 0.0 8 2.53196607165464E-4 0.0 0.0 0.0 0.0 9 2.53196607165464E-4 0.0 0.0 0.0 0.0 10-11 2.53196607165464E-4 0.0 0.0 1.26598303582732E-4 0.0 12-13 2.53196607165464E-4 0.0 0.0 2.53196607165464E-4 0.0 14-15 2.53196607165464E-4 0.0 0.0 2.53196607165464E-4 0.0 16-17 2.53196607165464E-4 0.0 0.0 2.53196607165464E-4 0.0 18-19 5.06393214330928E-4 0.0 0.0 6.329915179136599E-4 0.0 20-21 7.595898214963919E-4 0.0 0.0 0.0024053677680719076 0.0 22-23 7.595898214963919E-4 0.0 0.0 0.006583111786302064 0.0 24-25 0.001012786428661856 0.0 0.0 0.017090770983668818 0.0 26-27 0.001012786428661856 0.0 0.0 0.034941131788834026 0.0 28-29 0.0017723762501582478 0.0 0.0 0.0692492720597544 0.0 30-31 0.002025572857323712 0.0 0.0 0.1535637422458539 0.0 32-33 0.002025572857323712 0.0 0.0 0.28193442207874414 0.0 34-35 0.002025572857323712 0.0 0.0 0.4461324218255475 0.0 36-37 0.002278769464489176 0.0 0.0 0.6645144955057602 0.0 38-39 0.002278769464489176 0.0 0.0 0.9594885428535257 0.0 40-41 0.002278769464489176 0.0 0.0 1.2571211545765286 0.0 42-43 0.002278769464489176 0.0 0.0 1.5206988226357767 0.0 44-45 0.002278769464489176 0.0 0.0 1.7863020635523483 0.0 46-47 0.002278769464489176 0.0 0.0 2.098873275098114 0.0 48-49 0.002278769464489176 0.0 0.0 2.437523737181922 0.0 50-51 0.002278769464489176 0.0 0.0 2.7450310165843774 0.0 52-53 0.0024053677680719076 0.0 0.0 3.0292442081276114 0.0 54-55 0.0025319660716546398 0.0 0.0 3.301303962526902 0.0 56-57 0.0025319660716546398 0.0 0.0 3.608811241929358 0.0 58-59 0.0025319660716546398 0.0 0.0 3.9761995189264465 0.0 60-61 0.002658564375237372 0.0 0.0 4.435624762628181 0.0 62-63 0.0027851626788201037 0.0 0.0 4.83086466641347 0.0 64-65 0.0027851626788201037 0.0 0.0 5.137359159387264 0.0 66-67 0.0030383592859855677 0.0 0.0 5.476895809596152 0.0 68-69 0.0030383592859855677 0.0 0.0 5.868337764273958 0.0 70-71 0.0031649575895683 0.0 0.0 6.339156855298139 0.0 72-73 0.0032915558931510317 0.0 0.0 6.797316115964046 0.0 74-75 0.0032915558931510317 0.0 0.0 7.245347512343335 0.0 76-77 0.0032915558931510317 0.0 0.0 7.628433978984681 0.0 78-79 0.0032915558931510317 0.0 0.0 8.08203570072161 0.0 80-81 0.0037979491074819596 0.0 0.0 8.554373971388785 0.0 82-83 0.0037979491074819596 0.0 0.0 9.015191796429928 0.0 84-85 0.0037979491074819596 0.0 0.0 9.611596404608179 0.0 86-87 0.0037979491074819596 0.0 0.0 10.231295100645651 0.0 88-89 0.0037979491074819596 0.0 0.0 10.73034561336878 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATAGTC 45 2.0498192E-7 63.30818 7 AGATAGT 65 6.712071E-10 58.438324 6 TAGATAG 65 2.5830905E-6 43.85095 5 TGGGACG 55 4.9690025E-5 43.16467 6 GTTCGAC 40 2.2684617E-7 41.545994 16-17 TAGTCAA 80 2.2646054E-7 41.545994 9 ATGGGCG 60 8.2755236E-5 39.58766 5 CATATAG 60 8.2755236E-5 39.58766 3 TCGACCG 30 8.307816E-5 39.56761 18-19 GGGATAG 85 3.643945E-7 39.102116 7 AGTATCC 50 0.0016176853 37.98491 8 AAGTATC 55 0.0025826097 34.531734 7 AGTCTGG 55 0.0025826097 34.531734 6 TACCTGG 140 4.5110937E-10 33.94518 2 GTATATG 85 1.62405E-5 33.545826 1 ATAGTCA 100 1.300361E-6 33.236794 8 GGACCCG 115 1.0370786E-7 33.030357 9 ACCTGGG 145 6.6029315E-10 32.762203 3 TACTAGT 60 0.003938389 31.68217 2 GGATAAG 90 2.4009334E-5 31.682169 1 >>END_MODULE