##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938522_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 392712 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.153555277149668 31.0 31.0 33.0 28.0 34.0 2 30.999197885473325 31.0 31.0 34.0 27.0 34.0 3 30.95751594043472 31.0 31.0 34.0 27.0 34.0 4 34.666052985393875 37.0 35.0 37.0 32.0 37.0 5 34.79187801747846 37.0 35.0 37.0 32.0 37.0 6 34.979585548697266 37.0 35.0 37.0 32.0 37.0 7 34.95413432744607 37.0 35.0 37.0 32.0 37.0 8 34.992854814724275 37.0 35.0 37.0 32.0 37.0 9 36.36337315895618 39.0 37.0 39.0 32.0 39.0 10-11 36.24236718001996 39.0 35.0 39.0 32.0 39.0 12-13 36.058758072073175 38.5 35.0 39.0 31.0 39.0 14-15 36.63065809040722 39.0 36.0 41.0 30.5 41.0 16-17 36.65963352278514 39.0 36.0 40.0 30.5 41.0 18-19 37.00719738637984 39.0 36.0 40.5 31.5 41.0 20-21 37.165063710810976 39.0 36.0 41.0 32.0 41.0 22-23 37.019558862474284 39.0 35.5 41.0 31.5 41.0 24-25 36.79953248181874 39.0 35.0 41.0 31.0 41.0 26-27 36.40446052068691 38.5 35.0 41.0 30.5 41.0 28-29 36.02768440994927 38.0 35.0 40.5 30.0 41.0 30-31 35.48736860600134 37.5 35.0 40.0 30.0 41.0 32-33 34.772561062559845 35.5 34.5 40.0 28.5 41.0 34-35 34.32334764407505 35.0 34.0 40.0 26.5 41.0 36-37 33.82379835604718 35.0 33.0 39.0 24.0 41.0 38-39 33.34973211921204 35.0 33.0 39.0 22.5 41.0 40-41 32.74697106276355 35.0 33.0 39.0 20.0 41.0 42-43 32.247005439100406 35.0 33.0 39.0 16.5 41.0 44-45 31.474313237181445 35.0 31.5 39.0 5.5 41.0 46-47 30.518847144981564 35.0 30.5 38.0 2.0 40.0 48-49 29.749107488439364 35.0 27.5 38.0 2.0 40.0 50-51 28.829083399539613 34.0 25.5 37.5 2.0 39.5 52-53 28.83647176556866 35.0 26.5 38.0 2.0 40.0 54-55 28.9768176169814 35.0 27.0 38.0 2.0 40.0 56-57 28.703975177738393 35.0 25.5 38.0 2.0 40.0 58-59 28.3934931960317 35.0 25.5 38.0 2.0 40.0 60-61 27.879317413269774 35.0 24.0 37.0 2.0 40.0 62-63 27.296863859520464 34.0 22.0 36.0 2.0 40.0 64-65 26.718690796308742 34.0 20.5 36.0 2.0 39.0 66-67 26.122955244555808 33.0 16.5 35.0 2.0 39.0 68-69 25.618775336633462 33.0 10.5 35.0 2.0 38.5 70-71 24.959415551345515 33.0 4.0 35.0 2.0 37.5 72-73 24.46526334820428 33.0 2.0 35.0 2.0 37.0 74-75 24.063395057955958 32.5 2.0 35.0 2.0 36.0 76-77 23.391978090814643 31.5 2.0 35.0 2.0 36.0 78-79 22.68184063639512 31.0 2.0 35.0 2.0 35.0 80-81 22.11182495059993 30.5 2.0 35.0 2.0 35.0 82-83 21.54643988469922 29.5 2.0 35.0 2.0 35.0 84-85 20.951705066308136 29.0 2.0 34.0 2.0 35.0 86-87 20.321963678217116 29.0 2.0 34.0 2.0 35.0 88-89 19.526855558271713 26.5 2.0 34.0 2.0 35.0 90-91 18.720968546924972 25.0 2.0 33.5 2.0 35.0 92-93 17.99650252602416 24.0 2.0 33.0 2.0 35.0 94-95 17.215753529303917 19.0 2.0 33.0 2.0 35.0 96-97 16.201444060787548 3.5 2.0 33.0 2.0 35.0 98-99 15.146531045651777 2.0 2.0 32.5 2.0 35.0 100-101 12.734143596325042 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5090251587317134 1101 2 0.681387946698436 1101 3 0.7586405953992106 1101 4 0.3161500249419902 1101 5 0.3591216890178188 1101 6 0.13362176213718868 1101 7 0.370157788668692 1101 8 0.21482717836631338 1101 9 0.3566984145399701 1101 10-11 0.5448057734874254 1101 12-13 0.5039342107154567 1101 14-15 0.5294626487391128 1101 16-17 0.5447929429733236 1101 18-19 0.6432237833613712 1101 20-21 0.6247026459605394 1101 22-23 0.40731364628620526 1101 24-25 0.5700571136631964 1101 26-27 0.6553854379299722 1101 28-29 0.45860122545837356 1101 30-31 0.9239823856911968 1101 32-33 0.5237670906046716 1101 34-35 0.48002984270907234 1101 36-37 0.4108124235222661 1101 38-39 0.5121068457695941 1101 40-41 0.23189216434094817 1101 42-43 0.441963647009068 1101 44-45 0.190615801829928 1101 46-47 0.2739833451355622 1101 48-49 0.44891263975615914 1101 50-51 0.23113615073490124 1101 52-53 0.3802383887352718 1101 54-55 0.8687281241187677 1101 56-57 0.7746176430130767 1101 58-59 0.5119951852269118 1101 60-61 0.6164863425393463 1101 62-63 0.13305120937070924 1101 64-65 0.5155130132833747 1101 66-67 0.3319472740725473 1101 68-69 0.6187936034204995 1101 70-71 0.5939676899758659 1101 72-73 0.5681251814354802 1101 74-75 0.28826234145426 1101 76-77 0.3389132886491133 1101 78-79 0.5617653448928088 1101 80-81 0.805548407865075 1101 82-83 0.3839094165275547 1101 84-85 0.8171792500605335 1101 86-87 0.9255797728831858 1101 88-89 0.628184270294863 1101 90-91 0.46620834869482763 1101 92-93 0.9274091680403274 1101 94-95 0.573111345053622 1101 96-97 0.6033558329852298 1101 98-99 -0.03611851282876977 1101 100-101 0.029260167130814807 1102 1 0.4970950998403296 1102 2 0.6472521929494199 1102 3 0.526535683736288 1102 4 0.3079538012761489 1102 5 0.5698405281896939 1102 6 0.5341438919410848 1102 7 0.54692301239524 1102 8 0.4581184175139086 1102 9 0.47616596356553487 1102 10-11 0.4853679737181338 1102 12-13 0.6242517384915516 1102 14-15 0.580484141058939 1102 16-17 0.3195106286026217 1102 18-19 0.37573850620758975 1102 20-21 0.0913182127142349 1102 22-23 0.2701483254192354 1102 24-25 0.3767780170691779 1102 26-27 0.47751979528532473 1102 28-29 0.46653183076467286 1102 30-31 0.32647768045168135 1102 32-33 0.5863315979205552 1102 34-35 0.6909915329525305 1102 36-37 0.8843568349119764 1102 38-39 0.8643699141928778 1102 40-41 1.126130522667701 1102 42-43 1.3006470906372627 1102 44-45 1.4575546640726564 1102 46-47 1.4345154925461614 1102 48-49 0.9809082684368704 1102 50-51 0.8624502785959898 1102 52-53 0.8210813855174948 1102 54-55 1.0068269037921347 1102 56-57 0.8994886283813024 1102 58-59 1.2417550662298886 1102 60-61 1.1740648137918477 1102 62-63 0.9395747962810326 1102 64-65 0.9165891470943173 1102 66-67 0.9571294123762293 1102 68-69 0.9481755474433271 1102 70-71 0.6263351843599345 1102 72-73 1.0004000888485116 1102 74-75 1.1761258591520338 1102 76-77 1.1683635345358248 1102 78-79 0.818193309193699 1102 80-81 0.90321248412031 1102 82-83 0.7944567421301301 1102 84-85 0.39775268215329973 1102 86-87 0.4045959224794444 1102 88-89 0.6684444245052923 1102 90-91 1.2412447763555647 1102 92-93 1.6088948094707227 1102 94-95 1.476585633196418 1102 96-97 1.375053058411737 1102 98-99 1.1432877163012147 1102 100-101 0.40440435995456525 1103 1 0.6090310362443496 1103 2 0.5005956579950137 1103 3 0.523525396151534 1103 4 0.6435862539298824 1103 5 0.4493121204272441 1103 6 0.5499259821912617 1103 7 0.4908965920071182 1103 8 0.5230880223771308 1103 9 0.31521786910680305 1103 10-11 0.38005174739127057 1103 12-13 0.46954341490024376 1103 14-15 0.40845935733202765 1103 16-17 0.7003266211207375 1103 18-19 0.51143525626604 1103 20-21 0.49953366747224237 1103 22-23 0.295618865517433 1103 24-25 0.3839530229144046 1103 26-27 0.7798491149018858 1103 28-29 0.6692586252467905 1103 30-31 0.7964770959298235 1103 32-33 0.9118372093304217 1103 34-35 0.8271851265929371 1103 36-37 1.0799378496419294 1103 38-39 1.00563423497848 1103 40-41 1.27316699683184 1103 42-43 1.4377759192554507 1103 44-45 1.4609887300067221 1103 46-47 1.5121107696095244 1103 48-49 1.410414931986086 1103 50-51 1.4202010384744135 1103 52-53 1.3330786967169317 1103 54-55 1.3112166629691302 1103 56-57 1.4373071927956111 1103 58-59 1.3987722511726055 1103 60-61 1.351087259431317 1103 62-63 1.678322750454523 1103 64-65 1.5020608562362376 1103 66-67 1.250734743215606 1103 68-69 1.6354563517454075 1103 70-71 1.4542665614934371 1103 72-73 1.5227434369897317 1103 74-75 1.108145966704054 1103 76-77 1.2935388910941583 1103 78-79 1.4244739965914093 1103 80-81 1.2399847544032205 1103 82-83 1.5569801230045783 1103 84-85 1.557341763284935 1103 86-87 1.4203487866365627 1103 88-89 1.2440961663804373 1103 90-91 1.461667970183015 1103 92-93 1.4910405892228837 1103 94-95 1.3830913615106546 1103 96-97 1.3514529882691164 1103 98-99 0.743474763296776 1103 100-101 0.7908756014789979 1104 1 0.4215240740075217 1104 2 0.48176976961359586 1104 3 0.4788974580875909 1104 4 0.6123954329429111 1104 5 0.662829999451418 1104 6 0.6412148314727588 1104 7 0.5021614911939309 1104 8 0.6011625461601326 1104 9 0.4924624942599678 1104 10-11 0.34885396416747483 1104 12-13 0.48418499894837197 1104 14-15 0.5640653504254942 1104 16-17 0.616710156823082 1104 18-19 0.5499085769745164 1104 20-21 0.7332907689538004 1104 22-23 0.5008168715327983 1104 24-25 0.3487589157516524 1104 26-27 0.5168227697977343 1104 28-29 0.7147986163351234 1104 30-31 0.8365029954508216 1104 32-33 0.6290938095309357 1104 34-35 0.7035914984320755 1104 36-37 1.018670165560387 1104 38-39 0.697283728127637 1104 40-41 0.7607602908053295 1104 42-43 0.66999436639815 1104 44-45 0.9960018190119548 1104 46-47 1.392666634597827 1104 48-49 1.028661562263462 1104 50-51 0.5229775785211963 1104 52-53 0.485391551791011 1104 54-55 0.8233866529435048 1104 56-57 0.8885715350034751 1104 58-59 0.7275081874542124 1104 60-61 0.9072864351024741 1104 62-63 0.5420717757413662 1104 64-65 0.6121547071886724 1104 66-67 0.27216450817049065 1104 68-69 0.32546971924011814 1104 70-71 0.04767889300730488 1104 72-73 -0.051035386670733374 1104 74-75 0.22889900223931292 1104 76-77 0.5239889300826839 1104 78-79 0.37825170629398386 1104 80-81 0.43774012925005223 1104 82-83 0.564255660489259 1104 84-85 0.5325910113633618 1104 86-87 0.7282467480936212 1104 88-89 0.7317103470644106 1104 90-91 1.2244088635772457 1104 92-93 1.2326650183194658 1104 94-95 1.0944370226446658 1104 96-97 0.7852351538032565 1104 98-99 0.6917314191951807 1104 100-101 0.6706865071267654 1105 1 0.6963402990855272 1105 2 0.7388827845800137 1105 3 0.7301931373937407 1105 4 0.7991847925071411 1105 5 0.5735867819359513 1105 6 0.5741039319011279 1105 7 0.662640628112861 1105 8 0.45342866282415173 1105 9 0.79423678163635 1105 10-11 0.7195504579006169 1105 12-13 0.7577848720246863 1105 14-15 1.1209298065046127 1105 16-17 1.004270313362305 1105 18-19 0.8472669777360622 1105 20-21 0.8858570072530227 1105 22-23 1.013932319203228 1105 24-25 1.0712918365830006 1105 26-27 0.6588384766040107 1105 28-29 0.845153209386055 1105 30-31 1.039237143211146 1105 32-33 1.3511639877529475 1105 34-35 1.3068756488366446 1105 36-37 1.3635720641272115 1105 38-39 1.4421671169900776 1105 40-41 0.7244349959721816 1105 42-43 1.159219074209247 1105 44-45 1.3593473158653104 1105 46-47 1.2749250829557504 1105 48-49 1.4059554434840464 1105 50-51 1.015408431554146 1105 52-53 1.071997136433243 1105 54-55 1.235029256994487 1105 56-57 1.0768945557872307 1105 58-59 1.3699601944350128 1105 60-61 1.579353969081005 1105 62-63 1.3269096836159235 1105 64-65 1.3661673466725155 1105 66-67 0.794389374636193 1105 68-69 0.5536811529489327 1105 70-71 0.6704577284824786 1105 72-73 0.5123603508087733 1105 74-75 0.7847255927517658 1105 76-77 0.7332014743737609 1105 78-79 0.8238265648269554 1105 80-81 0.8935415994494278 1105 82-83 0.7095832822566699 1105 84-85 0.6381095475101652 1105 86-87 0.8034886963722165 1105 88-89 1.2118454679063362 1105 90-91 1.188242223353015 1105 92-93 1.2987049992809148 1105 94-95 1.4902802718910557 1105 96-97 1.539624597983277 1105 98-99 1.023643471656973 1105 100-101 0.9936345741847799 1106 1 0.5018371278381721 1106 2 0.4622208160575809 1106 3 0.11890584587389341 1106 4 0.6629386097493324 1106 5 0.6027152761807244 1106 6 0.31288711254477164 1106 7 0.38768173687489593 1106 8 0.4434451063289657 1106 9 0.4860972467526281 1106 10-11 0.3085215643135797 1106 12-13 0.28156922720438615 1106 14-15 0.7042631398379413 1106 16-17 0.5272618567026868 1106 18-19 0.08881031811660733 1106 20-21 0.6579089217468024 1106 22-23 0.38766969294059805 1106 24-25 0.5515302674028248 1106 26-27 0.2547687091621498 1106 28-29 0.31928405270294746 1106 30-31 0.22399166517497093 1106 32-33 0.07652809393100313 1106 34-35 0.1999928675352578 1106 36-37 -0.016507804324753295 1106 38-39 0.3074478311080071 1106 40-41 0.10019844441004722 1106 42-43 -0.39134470309871716 1106 44-45 -0.24136915113022894 1106 46-47 -0.7494759036545382 1106 48-49 -0.5573403046954226 1106 50-51 -0.5944987800972505 1106 52-53 -0.14990208837295782 1106 54-55 -0.13166090039334222 1106 56-57 -0.6170800742339146 1106 58-59 -0.5095617693892756 1106 60-61 0.041504544603672144 1106 62-63 -0.1461934406499914 1106 64-65 0.16317815240425304 1106 66-67 -0.5212084642174588 1106 68-69 -0.9614703622025829 1106 70-71 -0.6469312778600163 1106 72-73 -0.3540948993674071 1106 74-75 -0.37897537036780093 1106 76-77 -0.19274402715313244 1106 78-79 -0.07067073374011201 1106 80-81 -0.10757420980591803 1106 82-83 -0.24596052793830125 1106 84-85 0.09911495037603757 1106 86-87 0.6310668030200937 1106 88-89 0.6183230342676218 1106 90-91 0.8438976144735193 1106 92-93 0.8182610246508908 1106 94-95 0.790315507972803 1106 96-97 0.8487624885165168 1106 98-99 0.3464353438674497 1106 100-101 0.021265534955276166 1107 1 0.30694875329587745 1107 2 0.3691546980272804 1107 3 0.20748980070936085 1107 4 0.3552430049634552 1107 5 0.323694494128965 1107 6 0.1281754527972936 1107 7 0.17412992870168154 1107 8 -0.09621469013673334 1107 9 0.31543214761844496 1107 10-11 0.39181815860433744 1107 12-13 0.26428350767024256 1107 14-15 0.2691657201433699 1107 16-17 0.3538382677880776 1107 18-19 0.30332040298379326 1107 20-21 0.42669788043986756 1107 22-23 0.25009728641084905 1107 24-25 0.14207669942947376 1107 26-27 0.42838823963718653 1107 28-29 0.2005495902563652 1107 30-31 0.560827453900977 1107 32-33 0.6166685595371391 1107 34-35 0.6985171826582786 1107 36-37 0.6041891353129998 1107 38-39 0.5256440665807816 1107 40-41 0.19535868786743293 1107 42-43 0.32898545843060845 1107 44-45 0.5388915736086126 1107 46-47 -0.26864449912093846 1107 48-49 0.07661942931391152 1107 50-51 0.11371974372801574 1107 52-53 -0.10197600732662693 1107 54-55 0.24441218580869872 1107 56-57 0.41345925491117086 1107 58-59 0.2524641438820936 1107 60-61 -0.05826032552381122 1107 62-63 -0.03226260802082592 1107 64-65 -0.021805988575703594 1107 66-67 -0.26878981058090545 1107 68-69 0.05801357671263929 1107 70-71 0.42560996171527776 1107 72-73 0.4341014585688363 1107 74-75 0.23724246766200352 1107 76-77 -0.16559693627299055 1107 78-79 -0.4294825931030566 1107 80-81 -0.36271767576719327 1107 82-83 -0.050505759822890184 1107 84-85 -0.4449963934156784 1107 86-87 -0.8164667825880016 1107 88-89 -0.8963633980039525 1107 90-91 -0.5624698739230638 1107 92-93 -0.3052372669836281 1107 94-95 -0.19243551153562777 1107 96-97 -0.035426864508941946 1107 98-99 0.40609954901826306 1107 100-101 -0.10681901382804959 1108 1 0.15129363289871733 1108 2 -0.0051909695887530916 1108 3 0.13193622144086348 1108 4 0.08690762654826756 1108 5 0.0341784468031463 1108 6 0.014397604973893863 1108 7 0.16256864941855298 1108 8 -0.21756872194995225 1108 9 -0.4144594442807801 1108 10-11 -0.13154361825284155 1108 12-13 -0.11252751550860296 1108 14-15 0.20492294453575255 1108 16-17 0.13545448284813943 1108 18-19 -0.049785494732091706 1108 20-21 0.1312773628277739 1108 22-23 0.28289270692845747 1108 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>>Per sequence quality scores pass #Quality Count 2 2298.0 3 440.0 4 468.0 5 450.0 6 506.0 7 644.0 8 960.0 9 1272.0 10 1779.0 11 2756.0 12 4070.0 13 5632.0 14 6633.0 15 8713.0 16 10454.0 17 12484.0 18 10223.0 19 6699.0 20 6726.0 21 7315.0 22 7857.0 23 8583.0 24 8858.0 25 9359.0 26 10127.0 27 11225.0 28 12629.0 29 13813.0 30 15455.0 31 17931.0 32 20449.0 33 24403.0 34 32772.0 35 47894.0 36 28393.0 37 22117.0 38 9810.0 39 515.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.80146430439164 18.40992869807013 14.377734409877677 12.410872587660556 2 15.15160007439244 26.535936389367997 39.18468942429932 19.12777411194024 3 22.67002968115056 36.86016738640272 28.507917096597495 11.961885835849225 4 10.682901599288638 19.480645218822534 36.27381289061803 33.562640291270796 5 9.430794044728238 45.47455912592143 28.514825752498986 16.57982107685134 6 21.85857237138831 38.35430039165123 19.243657008314628 20.543470228645834 7 22.491842198441546 43.56380661743541 18.449750234990535 15.494600949132508 8 27.317812476120125 29.45260959270486 19.27940090170407 23.950177029470947 9 31.542719725281305 19.193879968513325 19.99403893037012 29.269361375835253 10-11 23.836618340504558 31.306793430495162 21.717476645751653 23.139111583248628 12-13 31.05530569640649 30.999135151934315 23.989407840185248 13.956151311473949 14-15 26.160684718648902 28.988078559237845 31.195710319178744 13.655526402934509 16-17 15.268048642562752 34.062784975215116 31.36943486389966 19.299731518322474 18-19 14.40426453355072 30.76731033779997 40.16698935140368 14.661435777245632 20-21 17.804982133871757 27.78313513926663 39.34802963038452 15.063853096477093 22-23 16.146985091053615 29.16887464460047 38.86427559640881 15.819864667937104 24-25 16.28396181700499 30.122112194400984 39.72303058411013 13.870895404483901 26-27 16.542970193098984 28.476304035995547 41.52201440535245 13.458711365553016 28-29 14.220241332794945 30.77483545780752 41.99605888489625 13.008864324501278 30-31 14.080649393995756 32.69913296933162 40.37934821103267 12.840869425639953 32-33 13.427719384511908 32.928663966889296 39.66150447832683 13.982112170271959 34-35 14.217107509184574 31.657987393823277 39.57824848537347 14.546656611618674 36-37 15.080188018292295 31.97164439575558 40.19515177763907 12.753015808313059 38-39 15.696255731023943 31.68785022924096 39.96867040244524 12.647223637289862 40-41 13.24462069808668 33.32454612140106 40.44919208590582 12.981641094606436 42-43 12.565234334208645 34.76948625016873 38.462978480629 14.20230093499362 44-45 13.348510487133161 34.42068982201222 36.99342111126814 15.237378579586474 46-47 13.572819217952784 34.63428361148993 35.68528132078859 16.107615849768703 48-49 14.305741778495932 34.24974119786235 35.41406432044862 16.030452703193102 50-51 14.415864377601523 35.300124656684794 35.402116489698315 14.881894476015367 52-53 13.395717567244075 35.68736407299694 34.70525168460351 16.211666675155474 54-55 12.268155969307326 34.7305732338088 35.14415522567468 17.857115571209192 56-57 14.305087076602199 35.25835208310254 32.84144634892016 17.595114491375107 58-59 14.598539216535173 33.688809318861324 33.57751880733244 18.135132657271065 60-61 14.381233852309034 33.11007233910883 34.906389551385466 17.602304257196664 62-63 16.303916026218886 32.63839019694724 34.40044920472429 16.65724457210958 64-65 15.51484407230278 33.61269424114169 35.09060766727276 15.781854019282779 66-67 14.765152798061582 32.83977371549931 35.04506718378167 17.35000630265743 68-69 14.44062168143283 33.68226303042959 34.923868726714325 16.953246561423256 70-71 14.817833460470197 33.26766364042723 33.58119715807718 18.33330574102539 72-73 14.322134694386927 31.589865448238445 34.45892771185599 19.629072145518634 74-75 14.021982332123978 32.694484805804905 34.86763100958018 18.415901852490936 76-77 14.722728025161397 30.61821145251041 36.104949511670256 18.55411101065794 78-79 13.971017961937044 30.534274360407597 37.02588418644152 18.46882349121384 80-81 13.732785315568593 32.035483640956684 35.78005948743023 18.451671556044495 82-83 13.347203512194127 31.076628069541034 35.348583739294966 20.22758467896987 84-85 13.652626753928034 30.154065547887647 35.29196057959204 20.901347118592273 86-87 14.611497133165512 29.93833409094671 35.4799894570777 19.970179318810075 88-89 15.293759543953447 28.536277530206334 35.747717411100346 20.422245514739874 90-91 14.372997930265882 28.795414742875337 36.1767871358064 20.65480019105238 92-93 14.179413349090783 30.182734639350922 35.370213069429994 20.2676389421283 94-95 13.977861921664672 29.98154523328277 34.6568801637367 21.383712681315853 96-97 13.928411764181428 28.82991202719553 34.13668327115671 23.104992937466324 98-99 15.332305219819753 28.311877512712396 33.39225247275733 22.96356479471052 100-101 16.142092636076928 27.362367836664347 34.08239700374532 22.41314252351341 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8067.0 1 5209.0 2 3705.5 3 4792.5 4 3632.0 5 3141.0 6 5358.0 7 10483.5 8 14835.5 9 14221.0 10 9626.5 11 5776.0 12 4781.5 13 4433.0 14 4014.0 15 3416.5 16 2735.5 17 2366.5 18 2172.0 19 2039.0 20 1972.5 21 2019.0 22 2121.0 23 2206.5 24 2379.5 25 2611.5 26 2759.5 27 2971.0 28 3280.5 29 3593.0 30 4028.0 31 4503.5 32 5088.0 33 5710.0 34 6170.0 35 6584.5 36 7199.5 37 7898.5 38 8170.5 39 8241.0 40 8181.0 41 7687.0 42 7036.5 43 6649.0 44 6596.5 45 6773.0 46 8250.0 47 12471.5 48 24682.5 49 26536.0 50 17237.5 51 12269.0 52 7506.0 53 5302.0 54 4326.0 55 3893.5 56 3730.5 57 3433.5 58 3188.5 59 2955.0 60 2637.5 61 2393.5 62 2073.0 63 1485.0 64 966.0 65 780.5 66 626.5 67 463.0 68 357.0 69 285.0 70 272.0 71 239.5 72 196.0 73 228.5 74 214.0 75 125.5 76 78.0 77 70.5 78 64.5 79 47.5 80 40.5 81 24.5 82 14.0 83 12.0 84 9.5 85 10.0 86 6.0 87 3.0 88 1.5 89 1.0 90 1.0 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1825765446434028 2 0.05067326692334332 3 0.05271038318156817 4 0.05729389476257409 5 0.06442380166636111 6 0.06900731324736703 7 0.036413453115769316 8 0.031066022937929066 9 0.04201552282588767 10-11 0.04354336001955632 12-13 0.04010572633380187 14-15 0.03615881358349121 16-17 0.034121697325266355 18-19 0.04379799955183442 20-21 0.053346982012263434 22-23 0.049909348326509 24-25 0.07282690623153863 26-27 0.05576605756890546 28-29 0.046726354173032655 30-31 0.05678461569801788 32-33 0.053601621544541546 34-35 0.052455743649290065 36-37 0.0501639878587871 38-39 0.028519627615147997 40-41 0.024445395098698284 42-43 0.02266291837275153 44-45 0.004201552282588767 46-47 0.009548982460429017 48-49 0.009548982460429017 50-51 0.008530424331316589 52-53 0.009676302226568071 54-55 0.01145877895251482 56-57 0.010312901057263337 58-59 0.011713418484792928 60-61 0.012095377783210088 62-63 0.004583511581005928 64-65 0.007639185968343213 66-67 0.005220110411701196 68-69 0.008912383629733749 70-71 0.023044877671168692 72-73 0.019097964920858034 74-75 0.01998920328383141 76-77 0.011968058017071036 78-79 0.005220110411701196 80-81 0.006111348774674571 82-83 0.005856709242396464 84-85 0.005602069710118356 86-87 0.008021145266760375 88-89 0.030684063639511906 90-91 0.03806861007557701 92-93 0.023808796268003016 94-95 0.03323045896229297 96-97 0.0375593310110208 98-99 0.021262400945221943 100-101 0.12451873128399439 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 392712.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.585412856600236 #Duplication Level Percentage of deduplicated Percentage of total 1 78.13387628687835 27.022923694682145 2 10.432546404196486 7.216278490695514 3 4.215686502800038 4.374037745200095 4 2.1110644338339855 2.9204814004413366 5 1.2203207809319128 2.1102649013009507 6 0.7760233606352313 1.6103452988361509 7 0.5604078789667639 1.3567356503510029 8 0.4027187073518339 1.1142554207071635 9 0.28858033914409903 0.898260315443674 >10 1.598179211229798 9.977259868682935 >50 0.11925583964155002 2.854824573886157 >100 0.10306060215936422 7.522652428842798 >500 0.018403678957029324 4.410173126625883 >1k 0.016195237482185806 12.438714829375021 >5k 7.361471582811729E-4 1.3066048080962953 >10k+ 0.0029445886331246917 12.86618744683287 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17384 4.426653629122614 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12172 3.09947238688912 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10603 2.69994296074477 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10376 2.6421397869176393 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5132 1.3068100796512458 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4430 1.1280531279920145 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4389 1.117612907168612 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3490 0.8886919676505938 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3489 0.8884373281183158 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3265 0.8313980728880198 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3131 0.7972763755627533 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2777 0.7071339811363034 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2249 0.5726843080934629 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2065 0.5258306341542912 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1942 0.494509971684084 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1832 0.4664996231334922 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1779 0.45300372792275256 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1741 0.4433274256961845 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1674 0.4262665770335513 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1633 0.4158263562101489 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1554 0.39570983316017844 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1518 0.3865428099981666 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1469 0.37406547291653935 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1246 0.31728085721852145 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1120 0.28519627615147997 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1058 0.26940862515023734 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1005 0.25591272993949765 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 913 0.2324858929699118 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 874 0.2225549512110656 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 835 0.21262400945221946 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 831 0.211605451323107 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 816 0.2077858583389354 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 813 0.2070219397421011 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 804 0.20473018395159812 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 774 0.1970909979832549 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 761 0.19378068406363952 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 705 0.17952087025606553 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 691 0.17595591680417202 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 691 0.17595591680417202 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 686 0.1746827191427815 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 659 0.1678074517712726 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 657 0.16729817270671637 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 643 0.16373321925482287 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 626 0.15940434720609506 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 612 0.15583939375420156 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 606 0.15431155656053291 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 598 0.15227444030230805 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 598 0.15227444030230805 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 585 0.14896412638269266 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 523 0.13317647538145003 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 520 0.1324125567846157 No Hit ACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAA 501 0.12757440567133166 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 494 0.1257919289453849 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACAAAAAAAAAAAAAAAA 478 0.12171769642893521 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 470 0.11968058017071034 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 451 0.11484242905742631 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 447 0.11382387092831386 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 434 0.1105135570086985 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 431 0.10974963841186418 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 424 0.10796716168591741 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 422 0.10745788262136119 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 416 0.10593004542769255 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 405 0.10312901057263338 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 405 0.10312901057263338 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.546395322781071E-4 0.0 12-13 0.0 0.0 0.0 2.546395322781071E-4 0.0 14-15 0.0 0.0 0.0 5.092790645562142E-4 0.0 16-17 1.2731976613905354E-4 0.0 0.0 6.365988306952677E-4 0.0 18-19 3.8195929841716063E-4 0.0 0.0 0.0011458778952514821 0.0 20-21 5.092790645562142E-4 0.0 0.0 0.0029283546211982315 0.0 22-23 5.092790645562142E-4 0.0 0.0 0.008403104565177535 2.546395322781071E-4 24-25 5.092790645562142E-4 0.0 0.0 0.020371162582248567 2.546395322781071E-4 26-27 5.092790645562142E-4 0.0 0.0 0.036540772881908365 2.546395322781071E-4 28-29 5.092790645562142E-4 0.0 0.0 0.08110269103057711 2.546395322781071E-4 30-31 5.092790645562142E-4 0.0 0.0 0.17124508545702705 2.546395322781071E-4 32-33 5.092790645562142E-4 0.0 0.0 0.28277720059483796 2.546395322781071E-4 34-35 5.092790645562142E-4 0.0 0.0 0.4383619548167614 2.546395322781071E-4 36-37 5.092790645562142E-4 0.0 0.0 0.656206074680682 2.546395322781071E-4 38-39 5.092790645562142E-4 0.0 0.0 0.9282884149198395 2.546395322781071E-4 40-41 5.092790645562142E-4 0.0 0.0 1.2585558882845445 2.546395322781071E-4 42-43 5.092790645562142E-4 0.0 0.0 1.5251634785797226 2.546395322781071E-4 44-45 6.365988306952677E-4 0.0 0.0 1.7935535456008473 2.546395322781071E-4 46-47 0.0017824767259467496 0.0 0.0 2.112871519077594 2.546395322781071E-4 48-49 0.002291755790502964 0.0 0.0 2.4719132595897246 2.546395322781071E-4 50-51 0.002291755790502964 0.0 0.0 2.795687424881338 2.546395322781071E-4 52-53 0.002291755790502964 0.0 0.0 3.0752816313226994 2.546395322781071E-4 54-55 0.002291755790502964 0.0 0.0 3.3586954307482326 2.546395322781071E-4 56-57 0.002291755790502964 0.0 0.0 3.6590427590702603 2.546395322781071E-4 58-59 0.002291755790502964 0.0 0.0 4.020121615840616 2.546395322781071E-4 60-61 0.002291755790502964 0.0 0.0 4.491204750555115 2.546395322781071E-4 62-63 0.002291755790502964 0.0 0.0 4.863869706044124 2.546395322781071E-4 64-65 0.002291755790502964 0.0 0.0 5.158742284422171 2.546395322781071E-4 66-67 0.002291755790502964 0.0 0.0 5.507216484344761 2.546395322781071E-4 68-69 0.002291755790502964 0.0 0.0 5.898470125690073 2.546395322781071E-4 70-71 0.0024190755566420178 0.0 0.0 6.327537737578684 2.546395322781071E-4 72-73 0.0025463953227810713 0.0 0.0 6.777867750412517 2.546395322781071E-4 74-75 0.0025463953227810713 0.0 0.0 7.205534844873597 2.546395322781071E-4 76-77 0.0025463953227810713 0.0 0.0 7.5709425736926805 2.546395322781071E-4 78-79 0.0025463953227810713 1.2731976613905354E-4 0.0 8.00815865061419 2.546395322781071E-4 80-81 0.003437633685754446 2.546395322781071E-4 0.0 8.456069587891381 2.546395322781071E-4 82-83 0.0035649534518934997 2.546395322781071E-4 0.0 8.911365071604635 2.546395322781071E-4 84-85 0.0035649534518934997 2.546395322781071E-4 0.0 9.508367455030658 2.546395322781071E-4 86-87 0.0035649534518934997 2.546395322781071E-4 0.0 10.114027582554137 2.546395322781071E-4 88-89 0.0035649534518934997 2.546395322781071E-4 0.0 10.594150420664507 2.546395322781071E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGG 20 0.0020492247 71.45796 1 AGATAGT 35 3.400337E-6 67.89903 6 GATAGTC 35 3.410647E-6 67.864426 7 TACGCGA 25 0.005016688 57.006115 8 ATATGAG 90 2.237357E-10 47.541443 3 TAGATAG 55 4.9318583E-5 43.219494 5 GTACTGG 45 9.4803213E-4 42.34546 1 GATTGGC 75 6.905557E-6 38.004078 9 GATATGA 90 5.646507E-7 36.97668 2 CATGAGA 125 5.5842975E-9 34.229836 4 TAGTCAA 70 2.0495844E-4 33.932213 9 ATGAGAG 155 3.6379788E-11 33.73909 5 GGTAAAC 60 0.0038918722 31.75909 1 GGATATG 120 1.4635953E-7 31.75909 1 CTAGATA 75 3.059089E-4 31.694298 4 GATTGCT 90 2.4071784E-5 31.670063 7 CGTCACT 110 2.6661128E-6 30.315498 1 GGGAACG 220 0.0 30.230516 7 GTTCGAC 40 4.4794916E-4 29.70204 16-17 GGTCAGG 65 0.005756851 29.316084 1 >>END_MODULE