##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938521_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 392775 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.628168798930684 31.0 31.0 34.0 30.0 34.0 2 31.70976258672268 31.0 31.0 34.0 30.0 34.0 3 31.69053274775635 31.0 31.0 34.0 30.0 34.0 4 35.44125007956209 37.0 35.0 37.0 33.0 37.0 5 35.38996371968685 37.0 35.0 37.0 33.0 37.0 6 35.54049010247597 37.0 35.0 37.0 33.0 37.0 7 35.466088727643054 37.0 35.0 37.0 33.0 37.0 8 35.52880911463306 37.0 35.0 37.0 33.0 37.0 9 36.916412704474574 39.0 37.0 39.0 32.0 39.0 10-11 36.87675259372414 39.0 37.0 39.0 32.0 39.0 12-13 36.73561708357202 39.0 36.0 39.0 32.0 39.0 14-15 37.2494978040863 39.0 36.0 41.0 32.0 41.0 16-17 37.330849723123926 39.0 36.5 40.0 32.0 41.0 18-19 37.68379479345681 39.0 36.5 40.5 33.0 41.0 20-21 37.81902234103494 39.5 37.0 41.0 33.0 41.0 22-23 37.66510088473044 40.0 36.0 41.0 32.5 41.0 24-25 37.461866208389026 39.0 35.5 41.0 32.0 41.0 26-27 37.01500986569919 39.0 35.0 41.0 31.5 41.0 28-29 36.65831964865381 38.5 35.0 40.5 31.0 41.0 30-31 36.050054102221374 37.5 35.0 40.0 31.0 41.0 32-33 35.36313920183311 36.0 34.5 40.0 30.0 41.0 34-35 34.8595697282159 35.0 34.0 40.0 29.0 41.0 36-37 34.41700210043918 35.0 33.0 40.0 25.5 41.0 38-39 33.99478454585959 35.0 33.0 40.0 24.5 41.0 40-41 33.569888613073644 35.0 33.0 39.0 23.0 41.0 42-43 33.03768187893832 35.0 33.0 39.0 20.5 41.0 44-45 32.2868958054866 35.0 33.0 39.0 15.0 41.0 46-47 31.298212717204507 35.0 31.5 39.0 2.0 41.0 48-49 30.465288014766724 35.0 30.5 39.0 2.0 41.0 50-51 30.036007892559354 35.0 29.0 38.0 2.0 40.0 52-53 29.482888422124624 35.0 27.5 38.0 2.0 40.0 54-55 29.216980459550633 35.0 27.0 38.0 2.0 40.0 56-57 28.89857552033607 35.0 27.0 38.0 2.0 40.0 58-59 28.4958793202215 35.0 26.0 37.5 2.0 40.0 60-61 28.042105531156515 34.5 26.0 37.0 2.0 40.0 62-63 27.493552288205716 34.0 23.0 36.0 2.0 39.5 64-65 26.891402202278655 33.0 22.0 36.0 2.0 39.0 66-67 26.3656049901343 33.0 20.0 35.0 2.0 39.0 68-69 25.869784227611227 33.0 18.5 35.0 2.0 38.0 70-71 25.324001018394757 33.0 11.0 35.0 2.0 37.5 72-73 24.750247597224874 32.5 7.0 35.0 2.0 37.0 74-75 24.187118579339316 31.5 2.0 35.0 2.0 36.0 76-77 22.91460378079053 29.5 2.0 33.5 2.0 35.0 78-79 23.525214181146964 31.0 2.0 35.0 2.0 35.0 80-81 23.393739418241996 31.0 2.0 35.0 2.0 35.0 82-83 23.0134160779072 31.0 2.0 35.0 2.0 35.0 84-85 22.5296938450767 31.0 2.0 35.0 2.0 35.0 86-87 21.97464451658074 30.5 2.0 34.5 2.0 35.0 88-89 21.246142193367703 29.5 2.0 34.0 2.0 35.0 90-91 20.74197950480555 29.0 2.0 34.0 2.0 35.0 92-93 20.109431608427215 29.0 2.0 34.0 2.0 35.0 94-95 19.517345808669084 27.0 2.0 34.0 2.0 35.0 96-97 18.69338298007765 25.5 2.0 33.5 2.0 35.0 98-99 17.863583476545095 23.5 2.0 33.0 2.0 35.0 100-101 15.705874864744448 9.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2038757452798201 1101 2 0.41524809074737945 1101 3 0.40126998684843684 1101 4 0.47259835465125377 1101 5 0.17072656110612883 1101 6 0.26612148394310964 1101 7 0.4414476909356537 1101 8 0.32409003920902535 1101 9 0.1653984515999909 1101 10-11 0.4760922261856422 1101 12-13 0.4286247664154459 1101 14-15 0.5002639164423357 1101 16-17 0.28172432094186917 1101 18-19 0.46560846358535457 1101 20-21 0.35325624246861054 1101 22-23 0.33322416455441584 1101 24-25 0.6086825677352152 1101 26-27 0.6722106089232085 1101 28-29 0.5740017917721261 1101 30-31 0.5039446833502907 1101 32-33 0.8823016177845417 1101 34-35 0.2864810782734608 1101 36-37 0.6489071355735732 1101 38-39 1.1147399864586092 1101 40-41 1.417883084569965 1101 42-43 1.1481995939997205 1101 44-45 1.3986941971446853 1101 46-47 1.6300863948689361 1101 48-49 1.426036361213228 1101 50-51 1.6085895417627292 1101 52-53 1.5513528203241869 1101 54-55 1.443897175353161 1101 56-57 1.195959674141477 1101 58-59 1.279979803571205 1101 60-61 0.7348172037212777 1101 62-63 1.0258173579545442 1101 64-65 0.8225330103257242 1101 66-67 1.029940559186759 1101 68-69 1.2148502563594974 1101 70-71 1.4627645511482648 1101 72-73 1.0145603203875417 1101 74-75 1.2383495676716052 1101 76-77 0.7871432784511647 1101 78-79 0.8866699191436602 1101 80-81 1.237656200529603 1101 82-83 1.3906006454584166 1101 84-85 1.6550392944644798 1101 86-87 1.7569183551474055 1101 88-89 2.0068707262164835 1101 90-91 2.0560445461994767 1101 92-93 2.2960932378926913 1101 94-95 2.6189622900475698 1101 96-97 2.908906299356847 1101 98-99 3.021262809199719 1101 100-101 3.4296451702412742 1102 1 0.22137114122825707 1102 2 0.3478512376570073 1102 3 0.2847955595278293 1102 4 0.10679434185684045 1102 5 -0.11963562428368135 1102 6 0.030927144503351656 1102 7 0.11528698530417358 1102 8 0.15000842633706668 1102 9 0.07816379036125909 1102 10-11 0.30218023626608925 1102 12-13 0.02536639027250942 1102 14-15 0.026030450641556513 1102 16-17 0.3162950940994449 1102 18-19 0.5386998015065814 1102 20-21 0.4992369862248722 1102 22-23 0.17723566660604462 1102 24-25 0.28697244528360244 1102 26-27 0.45680561393112384 1102 28-29 0.13551030522888396 1102 30-31 0.45717721218618834 1102 32-33 0.45242859268035573 1102 34-35 0.10073586810254653 1102 36-37 0.21052065371515027 1102 38-39 0.14598291883559256 1102 40-41 -0.14742982939081628 1102 42-43 -0.435489139805334 1102 44-45 -0.14217685710235628 1102 46-47 0.16997202053531524 1102 48-49 0.361329243511058 1102 50-51 -0.04953943629936575 1102 52-53 0.07931637562126781 1102 54-55 0.014629946507564995 1102 56-57 0.16869076522092286 1102 58-59 0.43997463410979876 1102 60-61 0.4722408733926926 1102 62-63 0.5928588105731976 1102 64-65 0.041734328538382215 1102 66-67 -0.34205065272885093 1102 68-69 0.26971924282196724 1102 70-71 -0.20192318107363505 1102 72-73 -0.2269181455747855 1102 74-75 0.21336652996684435 1102 76-77 0.5113444351181542 1102 78-79 0.5345569017936533 1102 80-81 0.905195213808657 1102 82-83 1.0984533804265908 1102 84-85 0.9153135990103749 1102 86-87 1.027442732388856 1102 88-89 1.0308037170729953 1102 90-91 0.8692331346134239 1102 92-93 1.204779548512704 1102 94-95 1.0596588749721292 1102 96-97 1.025251490142285 1102 98-99 1.1931958646745002 1102 100-101 1.8998399943180377 1103 1 0.46145150285558145 1103 2 0.36488376638924436 1103 3 0.4775748309551382 1103 4 0.38309652521134296 1103 5 0.18736351891955394 1103 6 0.28263335184816185 1103 7 0.3694897211448662 1103 8 0.39834653606613557 1103 9 0.3859163238206449 1103 10-11 0.46291087709097667 1103 12-13 0.4819208855010402 1103 14-15 0.7393654581754632 1103 16-17 0.542482053061839 1103 18-19 0.6504600756815435 1103 20-21 0.6558018221039745 1103 22-23 0.570527538854698 1103 24-25 0.336932559917102 1103 26-27 0.3116154936841369 1103 28-29 0.17460535058001625 1103 30-31 0.4809828670448155 1103 32-33 0.42913997035045526 1103 34-35 0.4488243249820343 1103 36-37 0.8274807978933651 1103 38-39 0.8305044421611427 1103 40-41 0.643740732254237 1103 42-43 0.9244762928770314 1103 44-45 0.9784468318508814 1103 46-47 0.5210001766495935 1103 48-49 0.20363432224772282 1103 50-51 -0.08790324993129772 1103 52-53 0.3604343476719656 1103 54-55 0.19488225838158968 1103 56-57 0.05693787722493937 1103 58-59 0.260645594608512 1103 60-61 0.7182818814583989 1103 62-63 0.5935457252185152 1103 64-65 0.592750666766289 1103 66-67 0.7471670031933897 1103 68-69 0.7586473605281157 1103 70-71 0.7884674000709282 1103 72-73 0.9769551666833145 1103 74-75 1.026931986284982 1103 76-77 0.7102911659953364 1103 78-79 0.8664991714187344 1103 80-81 1.1051390788483921 1103 82-83 0.9854891590775772 1103 84-85 1.035656613162292 1103 86-87 0.8560833800090251 1103 88-89 1.0335070585803692 1103 90-91 1.249089547294016 1103 92-93 1.5651069039651802 1103 94-95 1.4364795680911655 1103 96-97 1.936144626904774 1103 98-99 2.1089195624911454 1103 100-101 1.9235939459237965 1104 1 0.3295167709208471 1104 2 0.26203171647836854 1104 3 0.34692900619166167 1104 4 0.2643662358928225 1104 5 0.2664530568326242 1104 6 0.3155141964937158 1104 7 0.2533235343904465 1104 8 0.3629564359701547 1104 9 0.4505536472815024 1104 10-11 0.2968749522315193 1104 12-13 0.43413533771549595 1104 14-15 0.46792023673023664 1104 16-17 0.44719890483751357 1104 18-19 0.7584334748314134 1104 20-21 0.6358923198415312 1104 22-23 0.6303451722017357 1104 24-25 0.5645755052071024 1104 26-27 0.4220981482844337 1104 28-29 0.4687161417496313 1104 30-31 0.2997611475878088 1104 32-33 0.3874972992960224 1104 34-35 0.6744927741798961 1104 36-37 0.693598993423322 1104 38-39 0.7148524470973783 1104 40-41 0.9434116495722122 1104 42-43 0.96997197366683 1104 44-45 0.9871332434784676 1104 46-47 1.1109905784046994 1104 48-49 1.11317086413721 1104 50-51 0.9868396542233704 1104 52-53 1.1651854788936866 1104 54-55 1.3671090511966142 1104 56-57 1.1357257560128176 1104 58-59 1.3925529029864094 1104 60-61 1.257826650414934 1104 62-63 1.5649086759932302 1104 64-65 1.2387948186927957 1104 66-67 1.1058739824886032 1104 68-69 1.0483635267148692 1104 70-71 1.118455059470353 1104 72-73 1.2309795736489022 1104 74-75 1.1637206877795663 1104 76-77 0.9445431884826547 1104 78-79 1.1858602025444718 1104 80-81 1.1470354178035542 1104 82-83 1.0508120714593154 1104 84-85 1.4525426682836446 1104 86-87 1.416230096038099 1104 88-89 1.6201860658476122 1104 90-91 1.5157836375175187 1104 92-93 1.6644693062687566 1104 94-95 1.4686474002589947 1104 96-97 1.9929593807783483 1104 98-99 2.611456241498413 1104 100-101 2.6517099475692074 1105 1 0.3115680529721274 1105 2 0.3876727421193955 1105 3 0.39051874978141043 1105 4 0.2746226461492327 1105 5 0.07927356965313237 1105 6 0.1129500939296122 1105 7 0.09178507285198378 1105 8 0.00654617955989778 1105 9 0.4095280062558615 1105 10-11 0.18661854197511474 1105 12-13 0.46618661976678055 1105 14-15 0.42048433929434736 1105 16-17 0.5613014689400799 1105 18-19 0.2725360389339784 1105 20-21 0.03973847368768446 1105 22-23 0.17649901835557813 1105 24-25 0.1658575564891578 1105 26-27 0.4387648149510994 1105 28-29 0.3212802443137335 1105 30-31 0.3702739681006264 1105 32-33 0.3567280685267846 1105 34-35 0.41808251776964056 1105 36-37 0.32436822419255407 1105 38-39 0.5699806522255884 1105 40-41 0.5421295982901597 1105 42-43 0.7635617172565787 1105 44-45 1.0448255511707742 1105 46-47 0.7238110912252118 1105 48-49 0.9221452231115634 1105 50-51 1.3073524747361915 1105 52-53 1.3908265045347115 1105 54-55 1.4901859742735368 1105 56-57 1.3088026790897445 1105 58-59 1.548963159396667 1105 60-61 1.7680830606713442 1105 62-63 2.1072163683009215 1105 64-65 1.9811025110004898 1105 66-67 1.8763868030014237 1105 68-69 1.7252866036379473 1105 70-71 1.5569165979318917 1105 72-73 1.3489282915976197 1105 74-75 1.1521822262411057 1105 76-77 1.3535175474570131 1105 78-79 0.9948345615188252 1105 80-81 1.0944713152394527 1105 82-83 0.9661966868439293 1105 84-85 0.934593950334925 1105 86-87 1.0380249678329676 1105 88-89 1.3740322196937669 1105 90-91 0.8503990221329012 1105 92-93 1.159341101140555 1105 94-95 1.2622371438487399 1105 96-97 1.5955234833424505 1105 98-99 1.7729947030368756 1105 100-101 2.336325332184595 1106 1 0.24167643256504334 1106 2 0.339179482801395 1106 3 0.2389591410053029 1106 4 0.23969005296921608 1106 5 0.15383079936500366 1106 6 0.2554956926607801 1106 7 0.28020696024294267 1106 8 0.20001696993790574 1106 9 0.36222427905522636 1106 10-11 0.4138522189510567 1106 12-13 0.17990589018707936 1106 14-15 0.23339076276777604 1106 16-17 0.07369242849598123 1106 18-19 0.23624209233075533 1106 20-21 0.4166417250754719 1106 22-23 0.2582859599363374 1106 24-25 0.3689767740413643 1106 26-27 0.25430803461802753 1106 28-29 0.5030275348239073 1106 30-31 0.3816802594038293 1106 32-33 0.09773388401713845 1106 34-35 0.06547215562319053 1106 36-37 0.38369944280211854 1106 38-39 0.4866803614510715 1106 40-41 0.6949976923953756 1106 42-43 0.9097221719221835 1106 44-45 0.30454667197437146 1106 46-47 0.09273786891487745 1106 48-49 0.6316416545152137 1106 50-51 0.7023828738903042 1106 52-53 0.9971838928508632 1106 54-55 1.2411706425262494 1106 56-57 0.8407944845351558 1106 58-59 1.606040875495001 1106 60-61 1.1486804701347317 1106 62-63 0.936432598805812 1106 64-65 0.8800253235830944 1106 66-67 0.7319511699059973 1106 68-69 0.32756654019623355 1106 70-71 0.11413309277725148 1106 72-73 0.5927691586343649 1106 74-75 0.9526514305762888 1106 76-77 1.1495656574226487 1106 78-79 1.0304346143869232 1106 80-81 1.3025865676846031 1106 82-83 1.4578897875576509 1106 84-85 1.2059526603534287 1106 86-87 1.4784095832175836 1106 88-89 1.7129960050493054 1106 90-91 1.4752734604094009 1106 92-93 1.0995010271252212 1106 94-95 1.2089725390377417 1106 96-97 1.2304759020577585 1106 98-99 1.536086164839677 1106 100-101 2.2136250942781697 1107 1 0.2967328881369653 1107 2 0.4620317164783678 1107 3 0.34308285234550695 1107 4 0.2801171516437364 1107 5 -0.02292423254466769 1107 6 0.07100870198821951 1107 7 0.2688912999582129 1107 8 0.33035570336942044 1107 9 0.3007367974646513 1107 10-11 0.3259042562608272 1107 12-13 0.26060053918069315 1107 14-15 0.5678286616386714 1107 16-17 0.6610267436653459 1107 18-19 0.39213310853104844 1107 20-21 0.5347934187426304 1107 22-23 0.433641875498445 1107 24-25 0.4428722085038075 1107 26-27 0.32695162813791256 1107 28-29 -0.04364649561300382 1107 30-31 0.06340583623249785 1107 32-33 0.37183795863668223 1107 34-35 0.4387784884656085 1107 36-37 0.3712546710790008 1107 38-39 0.41476087200580736 1107 40-41 0.07976696092752178 1107 42-43 -0.017756964062101588 1107 44-45 -0.09702426567903899 1107 46-47 0.07582574323986435 1107 48-49 0.19293276889911226 1107 50-51 0.3238359912197062 1107 52-53 0.8479693616775705 1107 54-55 0.6574936665812317 1107 56-57 0.7797733750604365 1107 58-59 0.2830290934625985 1107 60-61 0.4131380057262888 1107 62-63 0.5059343170188697 1107 64-65 0.12579115568913224 1107 66-67 -0.038814662200042704 1107 68-69 0.26988367323501805 1107 70-71 0.18905945507474797 1107 72-73 0.5164191340884621 1107 74-75 0.5206803947392764 1107 76-77 0.3511365950760599 1107 78-79 0.38842430510857184 1107 80-81 0.5320171027852432 1107 82-83 0.6813065769538191 1107 84-85 0.5886232543642294 1107 86-87 0.8343619641699647 1107 88-89 0.990057860719407 1107 90-91 1.0330913298252078 1107 92-93 1.308791650591104 1107 94-95 1.815808572420167 1107 96-97 1.8349923478113155 1107 98-99 2.19634635138852 1107 100-101 2.3282667241259833 1108 1 0.12704359397958598 1108 2 0.22207899780224594 1108 3 0.06613249773558039 1108 4 0.3364156994316474 1108 5 0.15181403155529694 1108 6 0.15886753346407545 1108 7 0.02730062113349163 1108 8 0.42356745615564506 1108 9 0.30394517301345303 1108 10-11 0.22858071691601367 1108 12-13 0.22746139699899715 1108 14-15 0.30765473391154785 1108 16-17 0.5309777737227606 1108 18-19 0.4353110889430667 1108 20-21 0.5369379645041974 1108 22-23 0.49422951480948285 1108 24-25 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50-51 -1.2731921631077192 2316 52-53 -1.3745019626185737 2316 54-55 -0.9234674039783783 2316 56-57 -0.6518654848630945 2316 58-59 -0.9693692381328134 2316 60-61 -0.4522425990703738 2316 62-63 -0.4839683593801567 2316 64-65 -0.7481226434071466 2316 66-67 -0.7423947803786994 2316 68-69 -0.2526658575261891 2316 70-71 -0.1823778059610568 2316 72-73 -0.029800886766594203 2316 74-75 0.19327337353663054 2316 76-77 -0.06947515327291143 2316 78-79 0.16871627591148908 2316 80-81 0.3952948226323052 2316 82-83 0.5497546131025857 2316 84-85 0.615213452487275 2316 86-87 0.24331225961660863 2316 88-89 0.2616515346853454 2316 90-91 0.32731274101742613 2316 92-93 0.054811810479876044 2316 94-95 0.4167383603937509 2316 96-97 0.13616544478733417 2316 98-99 0.43398104030058704 2316 100-101 0.6322639434516706 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 24.0 5 77.0 6 185.0 7 413.0 8 677.0 9 1096.0 10 1712.0 11 2627.0 12 3609.0 13 5074.0 14 6046.0 15 8006.0 16 10122.0 17 12530.0 18 10751.0 19 6715.0 20 5971.0 21 6403.0 22 6871.0 23 7643.0 24 8158.0 25 8392.0 26 9002.0 27 10015.0 28 11527.0 29 13078.0 30 14737.0 31 17042.0 32 19789.0 33 23776.0 34 32164.0 35 51060.0 36 34247.0 37 29186.0 38 13397.0 39 642.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.68588886767234 19.34313538285278 15.28101330278149 12.6899624466934 2 15.451326982833274 27.201110488513063 37.982272935662984 19.365289592990678 3 22.377244929654204 36.07698065554237 29.69188388233437 11.853890532469054 4 10.800330978295463 20.392591178155435 35.295525428044044 33.51155241550506 5 9.254587155963302 45.8789500509684 28.805555555555557 16.06090723751274 6 22.03220673413532 38.883839348227355 18.33950735153714 20.744446566100187 7 21.71777735344663 43.60078925593533 18.16434345363121 16.517089936986824 8 27.45414041117688 30.221118961237348 19.052383680224047 23.272356947361722 9 30.198714276621473 19.391254535039145 19.844440201132965 30.565590987206413 10-11 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39.93297861911 14.491209173169548 36-37 14.924922822316287 31.829795359791223 40.489226950128895 12.756054867763597 38-39 15.476478451842892 31.51951110828188 40.30428416831116 12.69972627156407 40-41 13.22376335941031 33.05979032329932 40.75302834518361 12.963417972106761 42-43 12.55386620938472 34.57278128504884 38.76724832624877 14.106104179317667 44-45 13.254032006538285 34.257129290272644 37.15200114063552 15.336837562553546 46-47 13.5288651263446 34.42365221819107 35.74412831773917 16.30335433772516 48-49 14.29304309082808 34.05843039908344 35.354465024505124 16.29406148558335 50-51 14.266819425879957 35.07873464451658 35.38259817961937 15.271847749984088 52-53 13.274011838839032 35.606645025778114 34.51174336452168 16.60759977086118 54-55 12.158742282477245 34.59690662593088 34.89465979250207 18.34969129908981 56-57 14.350073197123034 35.13143657310165 32.48526510088473 18.03322512889059 58-59 14.407357902106805 33.936350327795815 33.127872191458216 18.52841957863917 60-61 14.40850359620648 33.366176564190695 34.39959264209789 17.825727197504936 62-63 16.279803959009612 33.15180446820699 33.69549996817516 16.87289160460824 64-65 15.351027942206096 34.15594169690026 34.34421742728025 16.148812933613392 66-67 14.69776512701961 33.23819804773228 34.29554909439016 17.76848773085795 68-69 14.52052701928585 34.194131500222774 33.94118770288333 17.344153777608046 70-71 14.888549423970465 33.74209152822863 32.59410604035389 18.775253007447013 72-73 14.423015721469035 32.225574438291645 33.24422379224747 20.107186047991853 74-75 14.267965119979632 33.42880784163961 33.06320412449876 19.240022913881994 76-77 14.572974349182102 31.26624657883012 35.36757685697919 18.79320221500859 78-79 13.878047228056776 31.02666921265356 36.44669339952899 18.648590159760676 80-81 13.584749538539874 32.521163516007896 35.41378651899943 18.480300426452803 82-83 13.12775128509997 31.803773700196807 34.891323442922214 20.177151571781014 84-85 13.50363197975359 30.887104371360703 34.543017254768166 21.06624639411754 86-87 14.38324513486918 30.61668484951097 34.943140951626845 20.056929063993003 88-89 14.994736165407888 29.583641504221887 34.66136421789093 20.7602581124793 90-91 14.188511401016365 29.562952552625948 35.68406098194372 20.56447506441396 92-93 13.774096266677867 31.1134788879596 34.589273461677024 20.52315138368551 94-95 13.61975685825218 30.988988606708674 33.63401438482592 21.757240150213224 96-97 13.453249315766024 30.101202978804658 33.10012093437719 23.34542677105213 98-99 14.7705429317039 29.503150658774107 32.49786773598116 23.22843867354083 100-101 15.307481185013188 28.8253745175318 33.263700060085746 22.603444237369263 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8084.0 1 5148.0 2 3534.0 3 4702.0 4 3686.0 5 3256.0 6 5577.5 7 10898.5 8 14825.0 9 14006.0 10 9634.5 11 5745.0 12 4748.5 13 4370.5 14 3849.0 15 3246.5 16 2667.0 17 2307.5 18 2192.5 19 2040.5 20 1961.0 21 1990.0 22 2009.5 23 2098.0 24 2216.0 25 2411.5 26 2669.5 27 2930.5 28 3188.5 29 3524.0 30 3943.0 31 4392.0 32 5077.0 33 5750.5 34 6118.0 35 6550.5 36 7383.5 37 8083.5 38 8261.5 39 8289.5 40 8169.0 41 7736.0 42 7216.5 43 6815.5 44 6732.5 45 6938.5 46 8259.0 47 12595.0 48 26283.0 49 26710.0 50 15426.0 51 11956.5 52 8430.0 53 6315.5 54 4709.5 55 3683.5 56 3342.5 57 3338.0 58 3333.0 59 3079.5 60 2615.5 61 2271.5 62 1888.5 63 1291.0 64 835.5 65 673.5 66 540.0 67 378.5 68 284.5 69 231.0 70 220.0 71 215.0 72 190.5 73 189.0 74 156.0 75 96.5 76 69.5 77 61.5 78 58.0 79 44.5 80 32.0 81 18.0 82 5.5 83 5.5 84 5.0 85 2.0 86 2.5 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.03946279676659665 3 2.545986888167526E-4 4 0.0 5 0.09547450830628222 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.910954108586342E-4 26-27 0.012220737063204125 28-29 0.0 30-31 0.0 32-33 6.364967220418814E-4 34-35 0.016421615428680543 36-37 0.003182483610209407 38-39 0.012729934440837629 40-41 0.007001463942460697 42-43 0.0049646744319266755 44-45 0.002927884921392655 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.545986888167526E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0010183947552670103 84-85 0.0035643816434345367 86-87 0.0014002927884921392 88-89 1.272993444083763E-4 90-91 7.637960664502578E-4 92-93 3.818980332251289E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.637960664502578E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 392775.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.376738730464155 #Duplication Level Percentage of deduplicated Percentage of total 1 76.12211387479526 24.645857925348867 2 11.335237244895978 7.3399602945164695 3 4.492806780496606 4.363872938957893 4 2.2927219255993183 2.9692343506694323 5 1.4152938174938057 2.2911299077919085 6 0.8725432343999343 1.6950062598720488 7 0.6276816545830017 1.4225599455437508 8 0.44201902283336447 1.144890753293673 9 0.314598084504446 0.9167093988394439 >10 1.7884778103678305 10.36918362421937 >50 0.13763566645975472 2.9955809353411835 >100 0.11718693887144832 8.090665849931607 >500 0.02123521711093359 5.09406635595889 >1k 0.01651627997517057 12.237727318221857 >5k 7.864895226271701E-4 1.329728293467499 >10k+ 0.0031459580905086803 13.093825848026095 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16410 4.17796448348291 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12797 3.2580994207879828 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11326 2.8835847495385396 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10888 2.772070523836802 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5222 1.329514353001082 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4440 1.1304181783463814 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3962 1.0087200050919738 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3791 0.965183629304309 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3471 0.8837120488829483 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3084 0.7851823563108651 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3024 0.7699064349818598 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2600 0.6619565909235567 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2396 0.6100184584049392 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2239 0.570046464260709 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2003 0.5099611736999554 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1985 0.5053783973012539 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1775 0.4519126726497359 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1762 0.448602889695118 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1744 0.44402011329641655 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1739 0.4427471198523328 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1633 0.415759658837757 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1462 0.3722232830500923 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1406 0.35796575647635415 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1269 0.32308573610845903 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1174 0.29889886067086757 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1100 0.28005855769842786 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 988 0.2515435045509516 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 949 0.2416141556870982 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 934 0.23779517535484693 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 923 0.23499458977786267 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 878 0.2235376487811088 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 850 0.2164088854942397 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 849 0.21615428680542295 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 836 0.2128445038508052 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 811 0.20647953663038635 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 809 0.20597033925275288 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 748 0.19043981923493092 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 746 0.18993062185729742 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 738 0.1878938323467634 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 716 0.18229266119279486 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 701 0.17847368086054358 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 694 0.17669149003882628 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 684 0.17414550315065877 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 671 0.17083572019604099 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 665 0.16930812806314047 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 661 0.16828973330787345 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 657 0.16727133855260645 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 655 0.16676214117497296 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 640 0.16294316084272165 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 567 0.14435745655909873 No Hit ACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAA 562 0.14308446311501496 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 551 0.14028387753803068 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 522 0.13290051556234483 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 495 0.12602635096429252 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACAAAAAAAAAAAAAAAA 481 0.12246196932085798 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 478 0.12169817325440775 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 475 0.12093437718795749 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 471 0.11991598243269047 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 469 0.11940678505505697 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 459 0.11686079816688945 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 450 0.11456940996753867 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 445 0.11329641652345492 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 442 0.11253262045700466 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 439 0.1117688243905544 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 410 0.10438546241486857 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 410 0.10438546241486857 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 405 0.1031124689707848 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 398 0.10133027814906753 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.091973776335052E-4 0.0 18-19 0.0 0.0 0.0 6.364967220418814E-4 0.0 20-21 0.0 0.0 0.0 0.002164088854942397 0.0 22-23 0.0 0.0 0.0 0.008401756730952836 0.0 24-25 0.0 0.0 0.0 0.01960409903888995 0.0 26-27 0.0 0.0 0.0 0.03984469479982178 0.0 28-29 2.545986888167526E-4 0.0 0.0 0.07561581057857553 0.0 30-31 2.545986888167526E-4 0.0 0.0 0.1703265228184075 0.0 32-33 2.545986888167526E-4 0.0 0.0 0.28960600852905605 0.0 34-35 2.545986888167526E-4 0.0 0.0 0.4494939851059767 0.0 36-37 2.545986888167526E-4 0.0 0.0 0.6648844758449494 0.0 38-39 7.637960664502578E-4 0.0 0.0 0.9350136846795238 0.0 40-41 7.637960664502578E-4 0.0 0.0 1.2325122525618992 0.0 42-43 8.910954108586341E-4 0.0 0.0 1.4941124053211126 0.0 44-45 0.001272993444083763 0.0 0.0 1.7590223410349437 0.0 46-47 0.001272993444083763 0.0 0.0 2.0810896823881357 0.0 48-49 0.001272993444083763 0.0 0.0 2.4202151358920503 0.0 50-51 0.001272993444083763 0.0 0.0 2.7333715231366558 0.0 52-53 0.001272993444083763 0.0 0.0 3.021449939532811 0.0 54-55 0.001272993444083763 0.0 0.0 3.2984533129654383 0.0 56-57 0.001272993444083763 0.0 0.0 3.6025714467570493 0.0 58-59 0.001272993444083763 0.0 0.0 3.959391509133728 0.0 60-61 0.001272993444083763 0.0 0.0 4.423270320157851 0.0 62-63 0.001272993444083763 0.0 0.0 4.802495067150405 0.0 64-65 0.001272993444083763 0.0 0.0 5.1109413786519 0.0 66-67 0.0015275921329005156 0.0 0.0 5.460123480364076 0.0 68-69 0.0015275921329005156 0.0 0.0 5.852969257208326 0.0 70-71 0.0015275921329005156 0.0 0.0 6.3140474826554644 0.0 72-73 0.0015275921329005156 0.0 0.0 6.769779135637451 0.0 74-75 0.0015275921329005156 0.0 0.0 7.20183311055948 0.0 76-77 0.0015275921329005156 0.0 0.0 7.569855515244097 0.0 78-79 0.0015275921329005156 0.0 0.0 8.016039717395454 0.0 80-81 0.0015275921329005156 0.0 0.0 8.493539558271275 0.0 82-83 0.0015275921329005156 0.0 0.0 8.972439691935588 0.0 84-85 0.0015275921329005156 0.0 0.0 9.580930558207626 0.0 86-87 0.0015275921329005156 0.0 0.0 10.197059385144167 0.0 88-89 0.0015275921329005156 0.0 0.0 10.691871936859526 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCACGC 25 0.0049932003 57.073742 3 AGATAGT 50 4.237918E-7 56.993816 6 TAGATAG 60 1.4765392E-6 47.56145 5 TAGTCAA 65 2.5869322E-6 43.841396 9 TTAGGCA 45 9.5563155E-4 42.27684 4 ATGGGCA 170 0.0 41.96598 5 ATGGGCG 80 2.2347194E-7 41.61627 5 GGAAGTC 140 0.0 40.709866 8 ATGGGGC 110 1.5934347E-9 38.913914 5 TATATGG 50 0.0016042965 38.04916 2 CTGTCGA 50 0.0016153819 37.995876 9 GGGAAGT 250 0.0 37.995876 7 GATAGTC 75 6.9158104E-6 37.995872 7 CATATAG 65 1.316538E-4 36.58573 3 GAAGTCT 170 0.0 36.319584 9 GGGAATG 95 8.687912E-7 34.996204 7 TAGGCAG 70 2.0353592E-4 33.97246 5 TCTAGAT 100 1.2832734E-6 33.293015 3 TACCTGG 175 3.6379788E-12 32.613564 2 GAGTAAT 75 3.0515518E-4 31.707634 1 >>END_MODULE