##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938520_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 391812 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.16631700917787 31.0 31.0 34.0 30.0 34.0 2 31.099228711729094 31.0 31.0 34.0 28.0 34.0 3 31.044161485610445 31.0 31.0 34.0 27.0 34.0 4 34.77664287974845 37.0 35.0 37.0 32.0 37.0 5 34.88171367900932 37.0 35.0 37.0 32.0 37.0 6 35.00063040437761 37.0 35.0 37.0 32.0 37.0 7 34.96540943105367 37.0 35.0 37.0 32.0 37.0 8 34.98993139566935 37.0 35.0 37.0 32.0 37.0 9 36.33114095535614 39.0 37.0 39.0 32.0 39.0 10-11 36.20959414208855 39.0 35.0 39.0 31.5 39.0 12-13 36.00519126519862 38.0 35.0 39.0 30.5 39.0 14-15 36.6940024807816 39.0 36.0 41.0 30.5 41.0 16-17 36.77804661419252 39.0 36.0 40.0 31.0 41.0 18-19 37.1061452941717 39.0 36.0 41.0 32.0 41.0 20-21 37.197430145069575 39.0 36.0 41.0 32.0 41.0 22-23 36.99122665972456 39.0 35.5 41.0 31.5 41.0 24-25 36.75355910487683 39.0 35.0 41.0 31.0 41.0 26-27 36.331296642267205 38.5 35.0 41.0 30.5 41.0 28-29 35.93469572141741 38.0 35.0 40.0 30.0 41.0 30-31 35.340135064775964 37.5 35.0 40.0 29.0 41.0 32-33 34.62966933120987 35.5 34.0 40.0 27.5 41.0 34-35 34.14856742519372 35.0 33.5 39.0 25.5 41.0 36-37 33.431730013373766 35.0 33.0 39.0 22.0 41.0 38-39 32.90812940900227 35.0 33.0 39.0 21.0 41.0 40-41 32.397102436882996 35.0 33.0 38.5 18.5 40.0 42-43 31.728682378283462 35.0 31.5 38.0 13.5 40.0 44-45 30.816017630904618 35.0 30.5 38.0 5.0 40.0 46-47 29.73448618214858 35.0 27.0 38.0 2.0 40.0 48-49 28.8784582912213 35.0 24.0 38.0 2.0 40.0 50-51 27.99647535042316 34.0 23.0 37.5 2.0 39.5 52-53 28.00914724408645 33.5 23.5 37.0 2.0 39.5 54-55 28.23606474533705 35.0 24.0 37.0 2.0 40.0 56-57 27.949277970046857 35.0 23.0 37.0 2.0 40.0 58-59 27.606173368860574 34.5 22.5 37.0 2.0 40.0 60-61 27.075476248813207 34.0 22.0 36.0 2.0 39.5 62-63 26.36339877288087 33.0 19.0 35.5 2.0 39.0 64-65 25.676762069563974 33.0 10.5 35.0 2.0 39.0 66-67 25.038811981256316 33.0 4.5 35.0 2.0 38.0 68-69 24.33715659551009 32.5 2.0 35.0 2.0 37.5 70-71 23.582548518166874 31.0 2.0 35.0 2.0 37.0 72-73 22.76571799740692 31.0 2.0 35.0 2.0 36.0 74-75 21.985614274192727 29.5 2.0 35.0 2.0 35.5 76-77 21.160319234735027 29.0 2.0 34.0 2.0 35.0 78-79 20.298839749675864 27.5 2.0 34.0 2.0 35.0 80-81 19.498346145600443 26.5 2.0 33.5 2.0 35.0 82-83 18.695515961736753 24.5 2.0 33.0 2.0 35.0 84-85 17.87458526027789 23.0 2.0 33.0 2.0 35.0 86-87 17.04232514573316 19.0 2.0 33.0 2.0 35.0 88-89 16.06968137780364 6.5 2.0 32.0 2.0 35.0 90-91 15.045275540310147 2.0 2.0 31.0 2.0 34.5 92-93 14.060800843261564 2.0 2.0 31.0 2.0 34.0 94-95 12.882821353097913 2.0 2.0 30.0 2.0 34.0 96-97 11.501107674088594 2.0 2.0 28.0 2.0 33.0 98-99 10.20269542535706 2.0 2.0 25.0 2.0 33.0 100-101 7.218987677763826 2.0 2.0 2.0 2.0 27.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.41906179741638283 1101 2 0.40357124920291554 1101 3 0.2502378503946794 1101 4 0.1909315924152608 1101 5 0.16892834694534997 1101 6 0.44994314313046146 1101 7 0.22863457914361618 1101 8 0.4285638548647981 1101 9 0.11298586010401124 1101 10-11 0.4036828489436317 1101 12-13 0.4496537118296544 1101 14-15 0.4878683870407343 1101 16-17 0.43617552092339196 1101 18-19 0.251005820393992 1101 20-21 0.5396992301823076 1101 22-23 0.5746039298021515 1101 24-25 0.613737122928228 1101 26-27 0.5101544565386718 1101 28-29 0.40861599612621546 1101 30-31 0.6768105108744891 1101 32-33 1.0785101988915144 1101 34-35 1.4073323134288245 1101 36-37 1.502426512319154 1101 38-39 1.753264198463647 1101 40-41 1.68756906835781 1101 42-43 1.6948609508351034 1101 44-45 1.8256436707694057 1101 46-47 1.850537690544762 1101 48-49 1.906478534953152 1101 50-51 2.373886338567825 1101 52-53 2.3035394780359866 1101 54-55 2.536348371769762 1101 56-57 2.191982444487131 1101 58-59 2.190322908006575 1101 60-61 2.297988424335557 1101 62-63 2.1779024509914855 1101 64-65 2.265395349752101 1101 66-67 2.5791953275365778 1101 68-69 2.418638174547162 1101 70-71 2.5005629215069263 1101 72-73 2.9621410367133727 1101 74-75 2.8694113348117405 1101 76-77 2.5682795838787484 1101 78-79 2.852106038397654 1101 80-81 3.05080372047415 1101 82-83 3.402686845635788 1101 84-85 3.9313820139919606 1101 86-87 3.5003349886734014 1101 88-89 3.2252413965372213 1101 90-91 3.3674925164940017 1101 92-93 3.0841622909412063 1101 94-95 3.169137688685103 1101 96-97 3.477173940215705 1101 98-99 2.776211215534458 1101 100-101 2.0149373708641596 1102 1 0.5011208174525663 1102 2 0.45208148499779455 1102 3 0.30638270547203916 1102 4 0.2598833899664754 1102 5 0.43090013563831064 1102 6 0.17734144204420232 1102 7 0.6175047005318106 1102 8 0.17601335152043163 1102 9 0.44429981036262944 1102 10-11 0.34590522130586265 1102 12-13 0.5970461552831807 1102 14-15 0.4787849167046474 1102 16-17 0.8445901175347288 1102 18-19 0.7947190830113868 1102 20-21 0.5137287627214064 1102 22-23 0.6393480536976739 1102 24-25 0.48569772619076446 1102 26-27 0.15534359541067033 1102 28-29 0.41074798420509495 1102 30-31 0.6835593041241879 1102 32-33 0.7338112671376535 1102 34-35 0.86993994504531 1102 36-37 0.7091392719675227 1102 38-39 0.8345462439710829 1102 40-41 1.3736638517433093 1102 42-43 1.1636918856818461 1102 44-45 0.6476639546841554 1102 46-47 0.80862610799403 1102 48-49 0.6318762182928666 1102 50-51 0.8055538685395227 1102 52-53 0.7256280756532618 1102 54-55 0.8530461924875787 1102 56-57 1.169495225155103 1102 58-59 1.4665504902269966 1102 60-61 1.4015625071677995 1102 62-63 1.1111764790226282 1102 64-65 1.035669880752259 1102 66-67 1.2710705011359558 1102 68-69 1.2263275949316714 1102 70-71 1.1506090895586318 1102 72-73 1.3519720541371214 1102 74-75 1.1376289477357915 1102 76-77 1.3284196645039295 1102 78-79 2.099686907558148 1102 80-81 2.0628749769207815 1102 82-83 2.189259074238958 1102 84-85 2.3653316720480966 1102 86-87 2.3984086924021284 1102 88-89 2.5905695650543628 1102 90-91 2.392659734925191 1102 92-93 2.178089878416527 1102 94-95 1.8695032795986304 1102 96-97 1.4192086375709874 1102 98-99 1.2977975948331526 1102 100-101 0.7800808671744681 1103 1 0.622684890236151 1103 2 0.6530106371464584 1103 3 0.7135965395151445 1103 4 0.7092962174280828 1103 5 0.6591231321539865 1103 6 0.6810838604207632 1103 7 0.5191049070100675 1103 8 0.5958094542433585 1103 9 0.8353180062588734 1103 10-11 0.78928501224663 1103 12-13 0.7547439227370489 1103 14-15 0.8016975505928912 1103 16-17 1.1073014351240928 1103 18-19 0.8301107464518225 1103 20-21 0.7687294079659495 1103 22-23 0.9230298190867643 1103 24-25 1.057126297619341 1103 26-27 1.1392124817185874 1103 28-29 0.9447846340953987 1103 30-31 0.9556911921097395 1103 32-33 1.2791396966124182 1103 34-35 1.4716304857602367 1103 36-37 1.829380593428354 1103 38-39 1.5596075422031177 1103 40-41 1.208029576352935 1103 42-43 2.048193111646487 1103 44-45 1.8772419647499667 1103 46-47 2.025170178196639 1103 48-49 1.791703292754086 1103 50-51 1.7528051267663933 1103 52-53 1.5620585828154745 1103 54-55 1.3563369396807659 1103 56-57 1.277444637982562 1103 58-59 1.3334365400398802 1103 60-61 1.5045757992054796 1103 62-63 1.3399156711764526 1103 64-65 1.1583953937216727 1103 66-67 1.1043715722419059 1103 68-69 1.0440137479836764 1103 70-71 0.5328842218337648 1103 72-73 0.6739168212038429 1103 74-75 0.4651550801399722 1103 76-77 0.7954154058899157 1103 78-79 0.9410857977375251 1103 80-81 0.9964793129641407 1103 82-83 0.6618361809624069 1103 84-85 1.1073629018842048 1103 86-87 1.2589713456477085 1103 88-89 1.625606343993585 1103 90-91 1.6232378740399138 1103 92-93 1.5872071838752948 1103 94-95 0.98871220978317 1103 96-97 1.0188021335059485 1103 98-99 0.8793565056603558 1103 100-101 0.4725250535210943 1104 1 0.29016010194728636 1104 2 0.3495509154561738 1104 3 0.3804688886931231 1104 4 0.19271395151606185 1104 5 0.382068789415996 1104 6 0.1325569727517859 1104 7 0.32981316409902206 1104 8 0.24951103019694898 1104 9 0.18103484527330238 1104 10-11 0.4566857527036703 1104 12-13 0.4498727934691331 1104 14-15 0.15535444379401753 1104 16-17 0.2077773419002682 1104 18-19 0.5584998977632267 1104 20-21 0.24043742390989564 1104 22-23 0.2732255720975303 1104 24-25 0.24679394305105973 1104 26-27 0.005093299342021851 1104 28-29 0.4908681553799994 1104 30-31 0.2431254091686199 1104 32-33 0.22786446504228763 1104 34-35 0.08777017964791867 1104 36-37 0.554478824558764 1104 38-39 0.5823248209525573 1104 40-41 0.6373102549633742 1104 42-43 0.6834612790862344 1104 44-45 0.6345933414042939 1104 46-47 0.5510790821216247 1104 48-49 0.37767858248272645 1104 50-51 0.5975538394775732 1104 52-53 0.7167460163232846 1104 54-55 0.6229227518429852 1104 56-57 1.159283581483706 1104 58-59 0.8053147759931782 1104 60-61 0.5669649788868831 1104 62-63 0.6103881735524403 1104 64-65 0.6249046068796815 1104 66-67 0.8418036615506743 1104 68-69 1.2733462018469623 1104 70-71 1.2332721816051055 1104 72-73 1.61166239902985 1104 74-75 1.3371283555985016 1104 76-77 1.36315233086545 1104 78-79 1.0961704112671775 1104 80-81 1.4413705849331642 1104 82-83 1.535710246525479 1104 84-85 1.1463716540296822 1104 86-87 1.2988170128392227 1104 88-89 1.1105205956638677 1104 90-91 0.8250797963912184 1104 92-93 0.6927116734280414 1104 94-95 0.48321906823104577 1104 96-97 0.5540270366933182 1104 98-99 0.10650092315330006 1104 100-101 -0.15930679747965737 1105 1 0.5777650610523608 1105 2 0.5154713574751995 1105 3 0.6045131994673376 1105 4 0.2437951833991292 1105 5 0.2568187247734386 1105 6 0.3184485637753198 1105 7 0.3981369484786512 1105 8 0.48144853785198904 1105 9 0.4900194807573044 1105 10-11 0.585453733542252 1105 12-13 0.5628509783060309 1105 14-15 0.273208038600842 1105 16-17 0.7409866068803339 1105 18-19 0.44557955100692936 1105 20-21 0.39352461651751014 1105 22-23 0.418477405904909 1105 24-25 0.6089351768393882 1105 26-27 0.5797826748268449 1105 28-29 0.5018778041327323 1105 30-31 0.5597991071313544 1105 32-33 0.7725460834198259 1105 34-35 0.864715920723846 1105 36-37 1.1131946830800103 1105 38-39 0.9633757048545206 1105 40-41 0.7435894291716778 1105 42-43 0.891705587138663 1105 44-45 0.7854282511088435 1105 46-47 0.9357669606208319 1105 48-49 1.177946678997472 1105 50-51 0.6842973514058279 1105 52-53 0.7381120837420738 1105 54-55 0.9568526112624269 1105 56-57 0.8676401634572777 1105 58-59 0.9490866353854024 1105 60-61 0.7574495939574497 1105 62-63 0.8454081106790454 1105 64-65 0.9989400718298036 1105 66-67 1.0185874560009403 1105 68-69 1.0898736034882006 1105 70-71 0.8443485142625242 1105 72-73 0.6378936696984532 1105 74-75 0.9446289339793097 1105 76-77 1.2328499397711532 1105 78-79 1.7248193137045398 1105 80-81 1.5796716423493677 1105 82-83 1.9380897732058493 1105 84-85 1.8825891796485514 1105 86-87 1.43318508077515 1105 88-89 1.096942255684823 1105 90-91 1.354155339728953 1105 92-93 1.539668709940882 1105 94-95 1.2495157979979243 1105 96-97 1.6077756247637023 1105 98-99 0.9130631637324971 1105 100-101 0.7526126102583968 1106 1 0.030039121710984773 1106 2 0.0745229606688298 1106 3 0.04280873807407559 1106 4 0.13326792783195174 1106 5 0.02938715065921116 1106 6 0.10922988153161128 1106 7 -0.03762051906209507 1106 8 -0.25424770988415446 1106 9 0.10966593638569577 1106 10-11 0.13536062842274532 1106 12-13 0.4265221259222898 1106 14-15 0.5198983429142814 1106 16-17 0.48441185494808536 1106 18-19 0.34328508374676403 1106 20-21 0.4677536296032372 1106 22-23 0.30119046357906143 1106 24-25 0.48430185796073033 1106 26-27 0.580433135250793 1106 28-29 0.5735368608375779 1106 30-31 0.7801840266528188 1106 32-33 0.7565332782773595 1106 34-35 0.5249460051561599 1106 36-37 0.7292636063766977 1106 38-39 0.6302251806599486 1106 40-41 0.5535401982454715 1106 42-43 0.21573053420903676 1106 44-45 0.16501017085988323 1106 46-47 0.537340819764264 1106 48-49 -0.12732970291207835 1106 50-51 -0.28580060028154364 1106 52-53 0.05755192252992458 1106 54-55 -0.08021087877660094 1106 56-57 -0.19794155222774634 1106 58-59 0.2504156153104091 1106 60-61 0.3335088241184039 1106 62-63 0.6312599587518939 1106 64-65 0.7776623634911566 1106 66-67 0.8311336897075456 1106 68-69 0.7534006294811135 1106 70-71 0.3696208148517677 1106 72-73 0.5404585244289599 1106 74-75 1.0518783791748305 1106 76-77 1.1030448228158143 1106 78-79 1.6185072967670635 1106 80-81 1.4693994693013863 1106 82-83 2.1095243572938003 1106 84-85 1.8958024541428493 1106 86-87 1.7628664827086737 1106 88-89 1.8329127171291013 1106 90-91 1.2057802153762243 1106 92-93 1.4768737707780613 1106 94-95 1.0432241876619823 1106 96-97 0.80886355170807 1106 98-99 0.7665437647069115 1106 100-101 0.33984445476009384 1107 1 0.1488431657977678 1107 2 0.10112974159014598 1107 3 0.08663766792620109 1107 4 0.2654263332240703 1107 5 0.17510979730533194 1107 6 0.14677440606357806 1107 7 0.24313288998792615 1107 8 0.09888429860698977 1107 9 0.10546234084235095 1107 10-11 0.26539025147835815 1107 12-13 0.3966981852163656 1107 14-15 0.24371432017529315 1107 16-17 0.24879162971691215 1107 18-19 0.24430869823927992 1107 20-21 0.40580306532600474 1107 22-23 0.41370110749075195 1107 24-25 0.482382955572902 1107 26-27 0.4869855872482489 1107 28-29 0.4174551395821382 1107 30-31 0.5438861890159501 1107 32-33 0.6479473894058003 1107 34-35 0.49973342675100696 1107 36-37 0.4415871573055625 1107 38-39 0.8691944902148805 1107 40-41 0.7030226942577471 1107 42-43 0.5328441452235388 1107 44-45 -0.13978648545270644 1107 46-47 -0.05250278969112898 1107 48-49 -0.2623874498808121 1107 50-51 0.164622757557769 1107 52-53 -0.053996271902860826 1107 54-55 -0.08179996981652948 1107 56-57 0.07435969146086308 1107 58-59 0.14015068684949128 1107 60-61 0.33459120499654205 1107 62-63 0.4419032707774164 1107 64-65 0.9642066601534651 1107 66-67 0.6494556095693831 1107 68-69 0.7370060072050535 1107 70-71 0.5328684372117785 1107 72-73 0.520960677197575 1107 74-75 0.6445883490720483 1107 76-77 0.6093052418561271 1107 78-79 1.080842840835853 1107 80-81 0.8939866054595029 1107 82-83 0.7335993863771613 1107 84-85 0.9646130944565918 1107 86-87 0.8206134514425614 1107 88-89 0.683700066765109 1107 90-91 0.29399275780195744 1107 92-93 0.20177512720218793 1107 94-95 0.5006485409319108 1107 96-97 -0.09966776837684144 1107 98-99 -0.0248055834659322 1107 100-101 0.12579688996715443 1108 1 0.34352685020854423 1108 2 0.44424597186005244 1108 3 0.1605714647509373 1108 4 -0.10632375036579589 1108 5 0.08631429847558536 1108 6 0.344184849608709 1108 7 0.35186313107263345 1108 8 0.21083130839564745 1108 9 0.39363408631408703 1108 10-11 0.3953926727021084 1108 12-13 0.2348451419686981 1108 14-15 0.4783136076437273 1108 16-17 0.5898181132929423 1108 18-19 0.31981629050520155 1108 20-21 0.14404571806487354 1108 22-23 0.2121947604256178 1108 24-25 0.3009151174951228 1108 26-27 0.3751798155119985 1108 28-29 0.6325578109990175 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70-71 -0.29292673598653707 2316 72-73 -0.6024640322798156 2316 74-75 -0.3606867830850682 2316 76-77 -0.836964804761319 2316 78-79 -0.4618712313877289 2316 80-81 -0.9111011647097911 2316 82-83 -0.7705242502298013 2316 84-85 -0.7127367371406699 2316 86-87 -0.1875808473998113 2316 88-89 -0.4041370175254819 2316 90-91 -0.4956181314983361 2316 92-93 -0.5892688582549521 2316 94-95 -0.5475274678116495 2316 96-97 -0.5592092891316423 2316 98-99 -0.6878133009954315 2316 100-101 -0.3750618524770273 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2270.0 3 514.0 4 452.0 5 444.0 6 553.0 7 774.0 8 1066.0 9 1524.0 10 2019.0 11 3022.0 12 4623.0 13 6201.0 14 7276.0 15 9049.0 16 11293.0 17 13325.0 18 10978.0 19 8065.0 20 8351.0 21 8893.0 22 9853.0 23 10935.0 24 11478.0 25 12326.0 26 13430.0 27 14679.0 28 15676.0 29 17288.0 30 19113.0 31 21051.0 32 23409.0 33 27646.0 34 33421.0 35 30290.0 36 17061.0 37 10936.0 38 2497.0 39 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.66367046572647 18.501967858314202 14.459658904558873 12.37470277140046 2 15.063201619677136 26.50332028706888 39.26644761197193 19.167030481282058 3 22.579310873089835 37.02901788563915 28.478890860578826 11.91278038069219 4 10.62649218001915 19.50616022981168 36.15703798276412 33.710309607405044 5 9.303768857696697 45.56749950836545 28.502800401480265 16.625931232457585 6 21.99769141584649 38.51019709792585 19.102256998534152 20.38985448769351 7 22.220349802119237 43.63385675986212 18.472615855993872 15.673177582024767 8 27.32537356116394 29.4451991134625 19.35470692173345 23.874720403640115 9 31.499350173499845 19.116899829691835 19.992952765656476 29.39079723115184 10-11 23.78451941870901 31.343779004227034 21.69576823938996 23.175933337673992 12-13 31.05365351743366 31.009611395292723 24.040615772696693 13.896119314576922 14-15 26.02071557318873 28.99031366962312 31.24901068713204 13.739960070056117 16-17 15.234473726535395 34.09346782161156 31.230230759215434 19.44182769263761 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34.775555320134735 38.388981284470184 14.303091259746742 44-45 13.370133309509072 34.3408869006975 36.872172198362506 15.416807591430922 46-47 13.706629497876706 34.508770787781785 35.56243961028932 16.22216010405219 48-49 14.466519800173074 34.25048310226963 35.159251440372294 16.12374565718501 50-51 14.465965886755464 35.25758511719089 35.18802615927342 15.088422836780225 52-53 13.374284963102692 35.713811666747496 34.591449422227214 16.320453947922598 54-55 12.34692848728653 34.66719588941967 34.9336791148877 18.05219650840609 56-57 14.410971911661102 35.16211290470794 32.61823444726927 17.808680736361687 58-59 14.587935613384229 33.644725037296816 33.42356035575226 18.343778993566698 60-61 14.533819674348914 33.03962283896888 34.80341731990025 17.623140166781955 62-63 16.46431037409431 32.570748124806165 34.26068799863181 16.704253502467715 64-65 15.454778656817563 33.45423498226677 35.116526302489795 15.974460058425871 66-67 14.800861690506089 32.513885060287706 35.08479075418339 17.600462495022818 68-69 14.390074877448322 33.47533715271707 34.9005228786531 17.23406509118151 70-71 14.824642686659367 32.91430861559747 33.65382406322512 18.607224634518037 72-73 14.356458389703745 31.37389790531813 34.317454320823785 19.95218938415434 74-75 14.195862209799493 32.48905565978737 34.574032239537466 18.741049890875676 76-77 14.78234679279139 30.404064510447785 35.817413532798255 18.996175163962565 78-79 14.05545863064889 30.39465808338832 36.57163204384211 18.978251242120685 80-81 13.853493429717892 31.79273746439183 35.35240604037124 19.001363065519037 82-83 13.604177143134285 30.782435781912493 34.85415480751804 20.75923226743518 84-85 13.948163727882543 29.868520465985316 34.79536567187038 21.387950134261764 86-87 14.85477792680639 29.642556786495028 34.970450220969305 20.532215065729275 88-89 15.55689717230869 28.360388325721857 35.228586818062304 20.85412768390715 90-91 14.733574331638174 28.657353511835463 35.38271245634459 21.22635970018177 92-93 14.381600862778631 29.84346183544667 34.72071370682118 21.054223594953513 94-95 14.37539330303065 29.69777733527863 33.79080937774833 22.136019983942386 96-97 14.39657923288021 28.701767821813775 33.05188588933563 23.849767055970386 98-99 15.673986792631034 28.0805920086994 32.46820046407029 23.777220734599275 100-101 16.83737094960646 26.563170806501073 33.08685985893898 23.51259838495349 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6903.0 1 4792.5 2 3735.5 3 4496.0 4 3448.5 5 3138.0 6 5055.5 7 9573.0 8 13899.0 9 13800.5 10 9586.0 11 5963.5 12 4984.0 13 4635.5 14 4150.5 15 3541.0 16 2967.5 17 2524.0 18 2312.5 19 2157.0 20 2032.5 21 2035.0 22 2077.0 23 2193.0 24 2375.0 25 2541.5 26 2810.0 27 3062.0 28 3239.0 29 3622.0 30 4062.5 31 4423.0 32 4967.5 33 5563.0 34 6092.0 35 6540.5 36 7231.0 37 7915.0 38 8138.5 39 8305.0 40 8247.0 41 7809.0 42 7270.5 43 6972.0 44 6922.5 45 7230.5 46 8839.0 47 12761.0 48 21996.0 49 24482.0 50 18079.5 51 13025.0 52 8444.0 53 5929.5 54 4746.0 55 4077.5 56 3742.0 57 3442.5 58 3227.5 59 3002.0 60 2702.0 61 2436.5 62 2091.5 63 1549.0 64 1071.0 65 828.5 66 659.5 67 514.0 68 394.5 69 308.0 70 264.5 71 226.0 72 187.5 73 197.5 74 183.0 75 125.0 76 93.5 77 75.0 78 68.5 79 54.5 80 34.5 81 21.5 82 14.0 83 7.5 84 5.0 85 7.0 86 5.0 87 1.5 88 1.0 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3930456443396323 2 0.03292395332455361 3 0.04007023776709238 4 0.04772697109838392 5 0.06661357998223638 6 0.05870162220656845 7 0.041346359988974304 8 0.04491950221024369 9 0.043132931099609 10-11 0.042494869988668034 12-13 0.03611425887925842 14-15 0.03215827999142446 16-17 0.026798566659520382 18-19 0.03126499443610711 20-21 0.03675231999019938 22-23 0.03611425887925842 24-25 0.04415382887711453 26-27 0.03930456443396323 28-29 0.03445529999081192 30-31 0.03726276887895215 32-33 0.03126499443610711 34-35 0.03330678999111819 36-37 0.03534858554612927 38-39 0.019141833328228844 40-41 0.01735526221759415 42-43 0.02003511888354619 44-45 0.013909732218512959 46-47 0.02182168999418088 48-49 0.018631384439476074 50-51 0.014803017773830305 52-53 0.012505997774442845 54-55 0.010847038885996344 56-57 0.01225077333006646 58-59 0.005742549998468654 60-61 0.010974651108184538 62-63 0.013399283329760191 64-65 0.008550018886608885 66-67 0.00612538666503323 68-69 0.009826141108490807 70-71 0.017610486661970536 72-73 0.014803017773830305 74-75 0.014547793329453922 76-77 0.00778434555347973 78-79 0.010847038885996344 80-81 0.01225077333006646 82-83 0.01556869110695946 84-85 0.010208977775055384 86-87 0.0039559788878339615 88-89 0.020162731105734383 90-91 0.027564239992649533 92-93 0.013909732218512959 94-95 0.024884383326697495 96-97 0.02220452666074546 98-99 0.01556869110695946 100-101 0.12557042663318121 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 391812.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.99796376071396 #Duplication Level Percentage of deduplicated Percentage of total 1 78.38078528439198 27.43167882919452 2 10.4501030992744 7.314646591282601 3 4.001384039486156 4.2012088081990715 4 2.0751253172934776 2.9050064261430872 5 1.2375859802567974 2.165649464389753 6 0.7766878301396424 1.6309495519568857 7 0.5805608951399447 1.4222914418296806 8 0.3448848550785157 0.965621412772558 9 0.29454679519840116 0.9277684257769272 >10 1.6002545895324718 10.066206304104561 >50 0.11883405145745438 2.8428845991576877 >100 0.10133701320605006 7.56775778235658 >500 0.018226081511879506 4.413844972251735 >1k 0.016038951730453967 12.19031569681896 >5k 7.290432604751801E-4 1.340559655714817 >10k+ 0.0029161730419007205 12.613610038050568 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16794 4.286239318857002 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11820 3.016752932528866 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10606 2.706910457055935 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10216 2.607372923749145 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5254 1.3409492307535247 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4455 1.1370248996967933 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4274 1.0908292752646678 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3454 0.8815452308760324 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3228 0.8238645064469694 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3227 0.8236092820025931 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3002 0.7661837820179066 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2656 0.6778761242636775 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2163 0.5520504731861199 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2040 0.5206578665278245 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1894 0.4833950976488724 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1794 0.45787265321123394 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1768 0.451236817657448 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1733 0.44230396210427453 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1685 0.430053188774208 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1557 0.39738445989403076 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1505 0.3841127887864588 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1500 0.3828366665645769 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1386 0.35374107990566905 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1273 0.32490071769113754 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1134 0.28942451992282014 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1028 0.26237072881892337 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1021 0.26058415770828863 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 943 0.2406766510469307 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 856 0.21847212438618524 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 846 0.21591987994242137 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 839 0.2141333088317867 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 831 0.2120915132767756 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 800 0.2041795555011077 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 793 0.20239298439047299 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 783 0.19984073994670914 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 775 0.19779894439169807 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 747 0.19065265994915928 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 724 0.18478249772850244 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 710 0.18120935550723305 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 700 0.1786571110634692 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 692 0.17661531550845813 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 638 0.16283319551213338 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 616 0.1572182577358529 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 609 0.15543168662521822 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 604 0.15415556440333628 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 570 0.14547793329453923 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 566 0.14445703551703368 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 564 0.14394658662828091 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 559 0.142670464406399 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACAAAAAAAAAAAAAAAA 520 0.13271671107571997 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 512 0.13067491552070892 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 502 0.12812267107694505 No Hit ACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAA 488 0.12454952885567568 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 486 0.12403907996692291 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 477 0.12174205996753545 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 476 0.12148683552315907 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 472 0.12046593774565353 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 460 0.1174032444131369 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 455 0.116127122191255 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 439 0.11204353108123283 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 437 0.11153308219248007 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 400 0.10208977775055385 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.552244443763846E-4 0.0 0.0 0.0 0.0 7 5.104488887527692E-4 0.0 0.0 0.0 0.0 8 5.104488887527692E-4 0.0 0.0 0.0 0.0 9 5.104488887527692E-4 0.0 0.0 0.0 0.0 10-11 5.104488887527692E-4 0.0 0.0 0.0 0.0 12-13 5.104488887527692E-4 0.0 0.0 0.0 0.0 14-15 5.104488887527692E-4 0.0 0.0 0.0 0.0 16-17 5.104488887527692E-4 0.0 0.0 0.0 0.0 18-19 6.380611109409615E-4 0.0 0.0 1.276122221881923E-4 0.0 20-21 7.656733331291537E-4 0.0 0.0 0.002041795555011077 0.0 22-23 7.656733331291537E-4 0.0 0.0 0.007273896664726961 0.0 24-25 0.0010208977775055385 0.0 0.0 0.016589588884464997 0.0 26-27 0.0010208977775055385 0.0 0.0 0.03381723887987096 0.0 28-29 0.0014037344440701151 0.0 0.0 0.0714628444253877 0.0 30-31 0.0015313466662583075 0.0 0.0 0.15951527773524038 0.0 32-33 0.0015313466662583075 0.0 0.0 0.2778118077036946 0.0 34-35 0.0017865711106346922 0.0 0.0 0.4291599032188907 0.0 36-37 0.0017865711106346922 0.0 0.0 0.640358130940349 0.0 38-39 0.0017865711106346922 0.0 0.0 0.9142139597562096 0.0 40-41 0.0017865711106346922 0.0 0.0 1.2275019652282215 0.0 42-43 0.0022970199993874616 0.0 0.0 1.4903831429358978 0.0 44-45 0.002552244443763846 0.0 0.0 1.7613038906414302 0.0 46-47 0.0026798566659520383 0.0 0.0 2.0859493838881913 0.0 48-49 0.0028074688881402307 0.0 0.0 2.4349688115728974 0.0 50-51 0.0028074688881402307 0.0 0.0 2.781308382591651 0.0 52-53 0.0028074688881402307 0.0 0.0 3.0649903525160025 0.0 54-55 0.0028074688881402307 0.0 0.0 3.3487999346625426 0.0 56-57 0.0028074688881402307 0.0 0.0 3.6581319612467205 0.0 58-59 0.0028074688881402307 0.0 0.0 4.020678284483375 0.0 60-61 0.0028074688881402307 0.0 0.0 4.4763815299174095 0.0 62-63 0.0028074688881402307 0.0 0.0 4.856410727593846 0.0 64-65 0.0028074688881402307 0.0 0.0 5.169826345288046 0.0 66-67 0.0028074688881402307 0.0 0.0 5.514506957418353 0.0 68-69 0.0028074688881402307 0.0 0.0 5.892621971761967 0.0 70-71 0.0028074688881402307 0.0 0.0 6.343348340530662 0.0 72-73 0.0028074688881402307 0.0 0.0 6.794967994854675 0.0 74-75 0.0028074688881402307 0.0 0.0 7.20192337141282 0.0 76-77 0.0028074688881402307 0.0 0.0 7.560386103539452 0.0 78-79 0.0028074688881402307 0.0 0.0 7.992863924535237 0.0 80-81 0.003062693332516615 0.0 0.0 8.443845517748308 0.0 82-83 0.003062693332516615 0.0 0.0 8.875940502077526 0.0 84-85 0.0031903055547048073 0.0 0.0 9.448536543035946 0.0 86-87 0.0033179177768929997 0.0 0.0 10.000459403999876 0.0 88-89 0.0033179177768929997 0.0 0.0 10.440976794993517 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGG 25 0.0049307784 57.25536 1 GATAGTC 45 1.51191325E-5 52.790764 7 AGATAGT 55 4.9426373E-5 43.20348 6 GGATATC 50 0.0016125643 38.00935 8 TCCGGCG 25 0.0016165902 37.99479 92-93 TAGTCAA 65 1.3246664E-4 36.54745 9 TACCTGG 150 2.5465852E-11 34.855263 2 ATGGGTG 220 0.0 34.562782 5 GGACTTA 55 0.0025712245 34.562782 6 TCGACCG 35 2.0513112E-4 33.932583 18-19 TATATGG 115 1.027729E-7 33.064278 2 AGTACTC 145 6.5847416E-10 32.775055 5 GTAAGCG 80 5.2750693E-11 32.68098 94-95 CCCTAGG 75 2.9951756E-4 31.808535 1 TAGATAG 75 3.065735E-4 31.682554 5 TGGGAGT 285 0.0 31.68255 6 CTATGGC 60 0.0039430456 31.674458 9 TATGCCC 30 0.003959023 31.652224 50-51 ATGGGAG 1270 0.0 30.684679 5 GGGAACT 140 1.6958438E-8 30.543226 7 >>END_MODULE