##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938520_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 391812 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.639385725807276 31.0 31.0 34.0 30.0 34.0 2 31.742435147468683 31.0 31.0 34.0 30.0 34.0 3 31.70348789725685 31.0 31.0 34.0 30.0 34.0 4 35.43604075423928 37.0 35.0 37.0 33.0 37.0 5 35.416033710044616 37.0 35.0 37.0 33.0 37.0 6 35.549743244208955 37.0 35.0 37.0 33.0 37.0 7 35.47121578716323 37.0 35.0 37.0 33.0 37.0 8 35.51900401212826 37.0 35.0 37.0 33.0 37.0 9 36.87380682572254 39.0 37.0 39.0 32.0 39.0 10-11 36.84093774565353 39.0 37.0 39.0 32.0 39.0 12-13 36.69220825293763 39.0 36.0 39.0 32.0 39.0 14-15 37.173479883209296 39.0 36.0 41.0 32.0 41.0 16-17 37.3547109327943 39.0 36.5 40.0 32.0 41.0 18-19 37.794956509754684 39.5 37.0 41.0 33.0 41.0 20-21 37.88037885516523 40.0 37.0 41.0 33.0 41.0 22-23 37.668531081232835 40.0 36.0 41.0 32.5 41.0 24-25 37.43188697640705 39.0 35.5 41.0 32.0 41.0 26-27 36.945720141292256 39.0 35.0 41.0 31.5 41.0 28-29 36.57965682521209 38.5 35.0 40.5 31.0 41.0 30-31 35.964884944820476 37.5 35.0 40.0 30.0 41.0 32-33 35.168455790021746 36.0 34.0 40.0 29.5 41.0 34-35 34.72780185395037 35.0 33.5 40.0 27.5 41.0 36-37 34.28484324114626 35.0 33.0 40.0 25.0 41.0 38-39 33.805678488662934 35.0 33.0 39.5 24.0 41.0 40-41 33.34567343521893 35.0 33.0 39.0 22.0 41.0 42-43 32.794666830010314 35.0 33.0 39.0 20.0 41.0 44-45 32.03845977152308 35.0 32.5 39.0 13.0 41.0 46-47 30.945416424203444 35.0 31.0 39.0 2.0 41.0 48-49 30.09701463967413 35.0 28.5 38.0 2.0 40.0 50-51 29.607272110093618 35.0 27.0 38.0 2.0 40.0 52-53 28.973308627607118 35.0 26.0 38.0 2.0 40.0 54-55 28.659069911079804 35.0 25.0 38.0 2.0 40.0 56-57 28.306834655395953 34.0 23.5 37.5 2.0 40.0 58-59 27.85273549559483 34.0 23.0 37.0 2.0 40.0 60-61 27.39611216603881 33.0 22.0 36.0 2.0 40.0 62-63 27.015395138484784 33.0 22.0 36.0 2.0 39.0 64-65 26.373965064878053 33.0 20.0 35.0 2.0 39.0 66-67 25.77538207099323 33.0 16.5 35.0 2.0 38.5 68-69 25.199685053035637 33.0 10.5 35.0 2.0 38.0 70-71 24.51913545271712 31.5 4.5 35.0 2.0 37.0 72-73 23.99238410257981 31.0 2.0 35.0 2.0 36.0 74-75 23.370925341745533 31.0 2.0 35.0 2.0 36.0 76-77 22.19883770788031 29.0 2.0 33.0 2.0 35.0 78-79 22.66605923248905 30.5 2.0 34.0 2.0 35.0 80-81 22.3687891131461 30.5 2.0 34.0 2.0 35.0 82-83 21.815234091860383 29.5 2.0 34.0 2.0 35.0 84-85 21.213245127765354 29.0 2.0 34.0 2.0 35.0 86-87 20.48721197921452 29.0 2.0 34.0 2.0 35.0 88-89 19.62455973783345 26.5 2.0 33.0 2.0 35.0 90-91 18.901124774126366 25.5 2.0 33.0 2.0 35.0 92-93 18.10707813951589 24.0 2.0 33.0 2.0 35.0 94-95 17.244700264412522 19.5 2.0 33.0 2.0 35.0 96-97 16.183114605984503 6.5 2.0 32.5 2.0 35.0 98-99 14.917648004655295 2.0 2.0 32.0 2.0 34.5 100-101 11.45376736802344 2.0 2.0 26.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20041154601297606 1101 2 0.3752064106086799 1101 3 0.25209436720661316 1101 4 0.2818565303094829 1101 5 0.22352724071831886 1101 6 0.12479758538317753 1101 7 0.3405324836522894 1101 8 0.3526745804656741 1101 9 0.5102396204310651 1101 10-11 0.32127868526348635 1101 12-13 0.2001357170011815 1101 14-15 0.19349645631690038 1101 16-17 0.27820233680341744 1101 18-19 0.368383142810913 1101 20-21 0.4330432004904097 1101 22-23 0.5339539479360482 1101 24-25 0.55865448845055 1101 26-27 0.49860923666336276 1101 28-29 0.5771863713200744 1101 30-31 0.5049574085192603 1101 32-33 0.2222483126545356 1101 34-35 -0.008810574316939324 1101 36-37 -0.47736661897306476 1101 38-39 -0.4103492000149416 1101 40-41 -0.3328172432239924 1101 42-43 -0.018171674583967956 1101 44-45 0.28760459506133884 1101 46-47 0.47766751009027786 1101 48-49 0.4829367852040498 1101 50-51 0.7545614984898918 1101 52-53 0.7633744348700091 1101 54-55 1.3247427445666844 1101 56-57 1.2946024012015585 1101 58-59 1.4558256192566255 1101 60-61 1.2516041208160047 1101 62-63 1.3853651355307193 1101 64-65 1.8011642187325165 1101 66-67 1.807988786117928 1101 68-69 1.9228180581427665 1101 70-71 1.880900334945519 1101 72-73 1.7893151488810517 1101 74-75 1.7641218935999206 1101 76-77 1.9156088377876657 1101 78-79 2.099711164580217 1101 80-81 2.2502782264608108 1101 82-83 2.4736890332428665 1101 84-85 2.3966433357279016 1101 86-87 2.3515607412360673 1101 88-89 2.014602482021864 1101 90-91 2.4078753755753333 1101 92-93 2.744406062560966 1101 94-95 2.74620020588463 1101 96-97 3.0433409829759555 1101 98-99 3.043423989448458 1101 100-101 2.4888790924556634 1102 1 0.4231067092526928 1102 2 0.41020404590359405 1102 3 0.274495256035447 1102 4 0.2902118216186338 1102 5 0.2610247071057188 1102 6 0.44570683325682126 1102 7 0.45000706870285256 1102 8 0.3390568819618167 1102 9 0.4824411984025261 1102 10-11 0.5329723395961494 1102 12-13 0.45243473583624905 1102 14-15 0.4154108915277348 1102 16-17 0.5860118691923759 1102 18-19 0.500915479214612 1102 20-21 0.4918417801659558 1102 22-23 0.37061288239242174 1102 24-25 0.01803410994759247 1102 26-27 0.3185988770029766 1102 28-29 0.279815322817349 1102 30-31 0.39926635160868784 1102 32-33 0.39095688339675405 1102 34-35 0.6663314956633215 1102 36-37 0.7832651701703028 1102 38-39 1.0793474421038383 1102 40-41 0.9836253394843837 1102 42-43 0.6891941940885715 1102 44-45 0.809367388866562 1102 46-47 0.7416849563589238 1102 48-49 0.8779176048183395 1102 50-51 1.5479012878434624 1102 52-53 1.1893178771299162 1102 54-55 1.0339590888076877 1102 56-57 1.3189438496021921 1102 58-59 1.1045392947590322 1102 60-61 0.7406551609108938 1102 62-63 0.7025246367656237 1102 64-65 1.0625429544035292 1102 66-67 0.760931154876797 1102 68-69 1.1339674549552967 1102 70-71 1.3484963682465718 1102 72-73 0.8456101439639667 1102 74-75 0.8547051124514979 1102 76-77 1.1079081569027416 1102 78-79 0.8932836709424024 1102 80-81 1.2471815888462352 1102 82-83 1.0950246787322868 1102 84-85 0.5586481552220768 1102 86-87 1.1623280317344573 1102 88-89 1.0762161868015738 1102 90-91 0.9110245621918551 1102 92-93 1.169358580784209 1102 94-95 1.0201231390197805 1102 96-97 1.3707550465902756 1102 98-99 1.851759011856851 1102 100-101 1.1705334851768754 1103 1 0.3816174848366387 1103 2 0.398202497316678 1103 3 0.17686975597092314 1103 4 0.23536603506553178 1103 5 0.38762168736123925 1103 6 0.38705410387238715 1103 7 0.5547302587918921 1103 8 0.5539233163404518 1103 9 0.5448363461635282 1103 10-11 0.5912701844793631 1103 12-13 0.41513960098013314 1103 14-15 0.5622362883531267 1103 16-17 0.51890643623328 1103 18-19 0.3369265774208259 1103 20-21 0.3604054658028062 1103 22-23 0.5186965060858029 1103 24-25 0.4714151913774529 1103 26-27 0.8232768999736848 1103 28-29 0.7038700395549924 1103 30-31 0.6742695778314243 1103 32-33 0.987066058774225 1103 34-35 0.9330433294483242 1103 36-37 1.0399757134662195 1103 38-39 0.6239983648035405 1103 40-41 0.5402132863162379 1103 42-43 0.4890393353971234 1103 44-45 0.6505404434542541 1103 46-47 0.7735277747871088 1103 48-49 0.6174323809184834 1103 50-51 0.5351899702540983 1103 52-53 0.5174892540817808 1103 54-55 0.5730609083973128 1103 56-57 0.3469152007442773 1103 58-59 0.6644502510114485 1103 60-61 0.9572314933408848 1103 62-63 0.8949688231122472 1103 64-65 0.38239067669027804 1103 66-67 0.3824952276603817 1103 68-69 0.6257083247449451 1103 70-71 0.5360689582581877 1103 72-73 0.6140964002551037 1103 74-75 0.4154871488432903 1103 76-77 0.49308688964245206 1103 78-79 0.6205710628639416 1103 80-81 0.8630675096102038 1103 82-83 0.7773062634528962 1103 84-85 0.8119066401878818 1103 86-87 0.6308078355801143 1103 88-89 0.5089817049329426 1103 90-91 0.716160708791417 1103 92-93 1.2186875264546195 1103 94-95 1.508540999388856 1103 96-97 1.1939257782975936 1103 98-99 1.5054562631150805 1103 100-101 1.0952011842348188 1104 1 0.052319784225268506 1104 2 0.1510004887205696 1104 3 0.23707799178237465 1104 4 0.16959018644785573 1104 5 0.1437815239500324 1104 6 0.20153557116886844 1104 7 0.05392171374742105 1104 8 0.11798987236410596 1104 9 0.18346635878818063 1104 10-11 -0.13737429684608315 1104 12-13 0.010852893744370817 1104 14-15 0.3686429439554928 1104 16-17 0.08483122735914606 1104 18-19 -0.025432813758939687 1104 20-21 -0.1345304889186636 1104 22-23 -0.1420762927566983 1104 24-25 -0.039156180453971956 1104 26-27 0.15910324719413182 1104 28-29 -0.16904653845541162 1104 30-31 0.039809955436155064 1104 32-33 0.15423274671838527 1104 34-35 0.05604501746341839 1104 36-37 0.07761915928659846 1104 38-39 -0.04024975923010743 1104 40-41 -0.06967791472778373 1104 42-43 -0.1273485960042109 1104 44-45 -0.4924337256593283 1104 46-47 -0.34838166816791016 1104 48-49 -0.2526613819366723 1104 50-51 -0.5212734253513922 1104 52-53 -0.46046016220543606 1104 54-55 0.10906244037739654 1104 56-57 0.16919487221965568 1104 58-59 0.3588095854512119 1104 60-61 -0.07764587732737027 1104 62-63 0.02825463669296724 1104 64-65 -0.021427925519745372 1104 66-67 -0.17672005809646052 1104 68-69 -0.187446844978588 1104 70-71 -0.031125390437502176 1104 72-73 0.2803587943995325 1104 74-75 -0.19328343713909035 1104 76-77 -0.2521185800938497 1104 78-79 0.08025374439648658 1104 80-81 0.15251954303006343 1104 82-83 0.008968603694324173 1104 84-85 -0.08748624177872699 1104 86-87 -0.18192533037020908 1104 88-89 -0.09571069332513815 1104 90-91 -0.17391262766261661 1104 92-93 0.29121898169384863 1104 94-95 0.4395416093438911 1104 96-97 0.2912597074009611 1104 98-99 0.13233400259335504 1104 100-101 0.2316289754883467 1105 1 0.02742899613038574 1105 2 0.1836347751347418 1105 3 -0.04207580307615899 1105 4 0.25036616935500433 1105 5 -0.09956709678186115 1105 6 0.0358736993924893 1105 7 0.12418477494691871 1105 8 0.16998970905658695 1105 9 0.23247902873006865 1105 10-11 0.2027170192764416 1105 12-13 0.09726298614920381 1105 14-15 0.4045535875232176 1105 16-17 0.052693667990055815 1105 18-19 0.20954629648724676 1105 20-21 0.0331353022382288 1105 22-23 -0.0011044769131416388 1105 24-25 -0.0706711298966809 1105 26-27 0.19372784402868604 1105 28-29 0.092228485020037 1105 30-31 0.2689167993822039 1105 32-33 0.529629107682446 1105 34-35 0.33833567763407757 1105 36-37 0.1454802390215022 1105 38-39 0.7213246613684134 1105 40-41 1.0911425694790182 1105 42-43 0.617010490018032 1105 44-45 0.7374794491749839 1105 46-47 0.667210797911757 1105 48-49 0.8543458716390795 1105 50-51 0.7674100438447304 1105 52-53 0.7491654553011315 1105 54-55 0.5589202267439397 1105 56-57 1.0642425716249448 1105 58-59 1.1794570997746447 1105 60-61 1.24563156854299 1105 62-63 1.2856034751682195 1105 64-65 1.1669030716087612 1105 66-67 0.7887880324303431 1105 68-69 0.7464748490647679 1105 70-71 0.7609501120733491 1105 72-73 0.7291959087556279 1105 74-75 0.8480469748041344 1105 76-77 0.9656179776557714 1105 78-79 0.8447404824648324 1105 80-81 0.7625854103971399 1105 82-83 0.6077322296656646 1105 84-85 0.8180369546938309 1105 86-87 0.8449659748651577 1105 88-89 1.068780807879257 1105 90-91 1.4328904915110456 1105 92-93 1.843120153425314 1105 94-95 1.1251848368448805 1105 96-97 1.3658095910443535 1105 98-99 1.582001263517947 1105 100-101 1.0770788196657541 1106 1 0.21908524692751996 1106 2 0.06610550441566687 1106 3 0.22503385689884325 1106 4 0.10003328666745404 1106 5 0.04794712406858537 1106 6 0.28100800033884354 1106 7 0.030123095993779714 1106 8 0.3363672822015005 1106 9 0.38193187672639084 1106 10-11 0.32528416974962937 1106 12-13 0.3766867547383441 1106 14-15 0.35957616232617085 1106 16-17 0.33709792276647477 1106 18-19 0.28954017450678293 1106 20-21 0.21202467294966 1106 22-23 0.4354628776260512 1106 24-25 0.4381802335193825 1106 26-27 0.002792201348277956 1106 28-29 0.21992917119201394 1106 30-31 0.5589775090217728 1106 32-33 0.32145626376743763 1106 34-35 0.019680217592750182 1106 36-37 0.13880983114455603 1106 38-39 0.12414459861811622 1106 40-41 0.26121246208928284 1106 42-43 0.3235890681880633 1106 44-45 0.13249386133731633 1106 46-47 -0.5496157204669387 1106 48-49 -0.2941759251621825 1106 50-51 -0.010680918581616794 1106 52-53 -0.32271042154502894 1106 54-55 -0.23275387982953433 1106 56-57 0.010630603153146012 1106 58-59 -0.05159026904916786 1106 60-61 0.3744099783585675 1106 62-63 -0.19262523181848934 1106 64-65 0.21529860256302769 1106 66-67 0.1836238868292419 1106 68-69 -0.019448677195978092 1106 70-71 0.01911912975056751 1106 72-73 0.21067054079909298 1106 74-75 -0.05615678511220068 1106 76-77 -0.17940918863252264 1106 78-79 -0.19688260760480603 1106 80-81 -0.1328180026069674 1106 82-83 0.09981829450763868 1106 84-85 0.311640760525016 1106 86-87 0.13105377426826337 1106 88-89 -0.15759855084328933 1106 90-91 0.15960145750854693 1106 92-93 0.33748920681408734 1106 94-95 0.15036785855239998 1106 96-97 -0.3053762875873858 1106 98-99 -0.05309043864770402 1106 100-101 0.468190469284405 1107 1 -0.008382641440334737 1107 2 0.07240789944570736 1107 3 0.06337832386373776 1107 4 0.09833026079824947 1107 5 0.11031744824315837 1107 6 0.11170084403224934 1107 7 -0.0863842617975692 1107 8 0.3051071629896924 1107 9 0.2557820344361872 1107 10-11 0.0801293982789062 1107 12-13 0.21884204191407264 1107 14-15 0.584729374688699 1107 16-17 0.10330365308873013 1107 18-19 0.17848369881087933 1107 20-21 0.0181885472135761 1107 22-23 0.10232343338815042 1107 24-25 0.28438320541945217 1107 26-27 0.30929488181505604 1107 28-29 0.33394795244913666 1107 30-31 0.30806003381758273 1107 32-33 0.5383881787120899 1107 34-35 0.6879049930212062 1107 36-37 0.6780753712556091 1107 38-39 0.7353809714128801 1107 40-41 0.8898868603334833 1107 42-43 0.9090491850012512 1107 44-45 1.3149814784203429 1107 46-47 1.470358854076423 1107 48-49 1.0364509562616426 1107 50-51 0.9619177549771081 1107 52-53 1.406883250874344 1107 54-55 1.4949838541866072 1107 56-57 1.0821786776545324 1107 58-59 1.1988954531177498 1107 60-61 1.635455660228537 1107 62-63 1.7442098984869752 1107 64-65 1.70988369988736 1107 66-67 1.8467335880801237 1107 68-69 2.0794467546170274 1107 70-71 1.2864762015055256 1107 72-73 1.6362201264361076 1107 74-75 1.3302501900979777 1107 76-77 1.4966321157413773 1107 78-79 1.0680998224452054 1107 80-81 0.7773443845076038 1107 82-83 1.0198780203444606 1107 84-85 1.131197973799022 1107 86-87 1.129914425975489 1107 88-89 1.0872923715491005 1107 90-91 1.3745814889305734 1107 92-93 0.758676287803759 1107 94-95 0.481432489583451 1107 96-97 0.40799366479984656 1107 98-99 0.6952694994676936 1107 100-101 0.24059965123233162 1108 1 0.04770904223254746 1108 2 -0.1035228304956064 1108 3 -0.031089756336417196 1108 4 0.025336129383447314 1108 5 -0.03784415114807871 1108 6 -0.053939684947494015 1108 7 -0.19268174948967953 1108 8 -0.3008040818652091 1108 9 -0.2616968951523262 1108 10-11 -0.15740706419789063 1108 12-13 -0.15260827111556807 1108 14-15 -0.2524635046075332 1108 16-17 -0.23603950349527025 1108 18-19 -0.3189855458825974 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-1.0413735751609678 2316 48-49 -0.8604887979372755 2316 50-51 -0.6982014652401425 2316 52-53 -0.49126933762073577 2316 54-55 -0.5140552464758521 2316 56-57 -0.4325314688893087 2316 58-59 -0.8027467524005054 2316 60-61 -0.5483932903212825 2316 62-63 -0.840208944775803 2316 64-65 -0.4184425330214374 2316 66-67 -0.2065865109540681 2316 68-69 -0.08791601387939352 2316 70-71 -0.16338216175368458 2316 72-73 -0.21220904404099628 2316 74-75 -0.020928071450434516 2316 76-77 -0.4704261895248365 2316 78-79 -0.7161495028285891 2316 80-81 -1.51346539846476 2316 82-83 -0.8315734774361907 2316 84-85 -1.0426834305236845 2316 86-87 -0.8563540592920589 2316 88-89 -0.9396102448272288 2316 90-91 -0.5829665225788041 2316 92-93 -0.5465982867283934 2316 94-95 -0.07704842849028637 2316 96-97 0.25571186344816255 2316 98-99 0.28290026252169476 2316 100-101 -0.5610503610354183 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 27.0 5 94.0 6 206.0 7 433.0 8 745.0 9 1207.0 10 1956.0 11 2766.0 12 3994.0 13 5065.0 14 6408.0 15 8276.0 16 10265.0 17 12986.0 18 10975.0 19 7250.0 20 6737.0 21 7212.0 22 7978.0 23 8716.0 24 9176.0 25 9616.0 26 10512.0 27 11655.0 28 13172.0 29 14611.0 30 16585.0 31 18870.0 32 21880.0 33 26297.0 34 36536.0 35 44174.0 36 28044.0 37 21356.0 38 5921.0 39 101.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.81449599460842 19.345607343983744 15.131649486115153 12.708247175292684 2 15.450649254512363 27.099196695910987 38.0524151451027 19.397738904473947 3 22.37490588430469 36.151657074309924 29.63846811551665 11.834968925868736 4 10.80926566822864 20.487376598981143 35.2357763417149 33.46758139107531 5 9.25576172124244 45.84339348153442 28.90355019014318 15.997294607079962 6 22.03071881412514 38.80713199187365 18.40244811287046 20.759701081130746 7 21.75762865864241 43.47595275285086 18.221238757363224 16.545179831143507 8 27.278133390503612 30.35333272079467 19.12090492378998 23.247628964911744 9 30.09920599472705 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13.958990536277602 34-35 14.101696905342903 31.49140316306423 39.89424971667194 14.512650214920924 36-37 14.994040463593231 31.898833360132105 40.312626815616795 12.794499360657872 38-39 15.564512217845994 31.50259212840416 40.18424404550711 12.748651608242739 40-41 13.279932815409573 33.05190851970548 40.63519048184268 13.032968183042273 42-43 12.634062798307275 34.54328039735167 38.68627390924823 14.136382895092831 44-45 13.349582705903371 34.2283249534213 37.092595901074496 15.329496439600826 46-47 13.581258358600554 34.422503649709554 35.628311537165786 16.36792645452411 48-49 14.298821883964758 34.054597613141 35.24930323726686 16.397277265627395 50-51 14.3275346339571 35.17133216950987 35.25644952170939 15.244683674823639 52-53 13.373122824211611 35.6497503904934 34.39991628638224 16.577210498912745 54-55 12.218997886741601 34.73898196073627 34.60971077965963 18.432309372862495 56-57 14.376282502832991 35.184859065061815 32.40201933580391 18.03683909630129 58-59 14.505426071687442 33.878237522076915 33.065985727849075 18.55035067838657 60-61 14.512827580574358 33.37225506110073 34.189866568660484 17.925050789664432 62-63 16.288296427878677 33.21286739558768 33.490806815513565 17.00802936102008 64-65 15.294089121937462 34.0823329585783 34.33130472178586 16.292273197698385 66-67 14.788256988444582 33.17726179309993 34.147253434403375 17.88722778405212 68-69 14.560011842595571 34.08906448599562 33.81597014849185 17.534953522916954 70-71 14.929353873796616 33.55474053882985 32.56549059242698 18.950414994946556 72-73 14.392799580409408 32.14832170143475 33.00728285740173 20.451595860754114 74-75 14.295631578410054 33.25548987779854 33.046716282298654 19.402162261492755 76-77 14.490623053913612 31.070002960603553 35.361984829459026 19.07738915602381 78-79 13.87655304074403 31.007472971731342 36.2419987136688 18.873975273855827 80-81 13.693446976415954 32.497387136416364 35.106677573271845 18.70248831389584 82-83 13.345363081010653 31.74909043111842 34.49700298489178 20.40854350297915 84-85 13.599898924412818 30.806869056066322 34.25390391693425 21.339328102586617 86-87 14.54716694593722 30.573297991676757 34.475343579350906 20.40419148303511 88-89 15.143231958184028 29.585872816554875 34.205307647545254 21.065587577715846 90-91 14.323378602219972 29.640358645965996 35.20738526876753 20.8288774830465 92-93 13.855004255873041 31.235811097938676 34.024652160541486 20.884532485646798 94-95 13.787642511312853 30.937875659437843 33.126303243145294 22.148178586104013 96-97 13.61903157636826 30.164466631956145 32.618194440190706 23.598307351484895 98-99 14.93701060712791 29.484803936581834 32.060273804783925 23.517911651506335 100-101 15.552396436036764 28.674794735216725 32.6735339232436 23.09927490550291 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7454.0 1 4895.0 2 3606.0 3 4660.0 4 3619.0 5 3233.5 6 5467.0 7 10371.0 8 14214.5 9 13601.0 10 9440.0 11 5799.0 12 4765.0 13 4382.0 14 3927.5 15 3309.0 16 2742.5 17 2395.0 18 2218.0 19 2076.5 20 2017.0 21 1988.0 22 1976.5 23 2134.5 24 2292.0 25 2472.0 26 2675.0 27 2910.0 28 3207.5 29 3476.0 30 3892.0 31 4365.5 32 4900.0 33 5599.0 34 6168.5 35 6687.5 36 7382.5 37 8041.0 38 8427.5 39 8486.5 40 8384.0 41 7927.0 42 7325.0 43 6841.5 44 6769.5 45 6987.0 46 8323.0 47 12782.5 48 24989.5 49 25957.0 50 16204.0 51 12406.0 52 8871.0 53 6521.0 54 4753.5 55 3728.5 56 3357.5 57 3285.5 58 3219.5 59 2988.5 60 2650.5 61 2308.5 62 1980.0 63 1371.5 64 863.0 65 672.5 66 551.5 67 417.0 68 285.5 69 218.5 70 209.5 71 190.0 72 163.5 73 181.0 74 167.5 75 111.5 76 71.0 77 56.0 78 49.5 79 41.0 80 34.5 81 20.0 82 8.5 83 7.0 84 6.0 85 2.5 86 2.5 87 1.0 88 0.5 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022970199993874615 2 0.014037344440701153 3 0.0017865711106346922 4 0.0 5 5.104488887527692E-4 6 0.0 7 0.0 8 0.0 9 2.552244443763846E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002169407777199269 26-27 0.009826141108490807 28-29 2.552244443763846E-4 30-31 0.0 32-33 0.0 34-35 0.010208977775055384 36-37 7.656733331291537E-4 38-39 0.012505997774442845 40-41 0.014164956662889345 42-43 0.004594039998774922 44-45 5.104488887527692E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.276122221881923E-4 66-67 0.0011485099996937306 68-69 0.0015313466662583075 70-71 0.0 72-73 3.8283666656457687E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.276122221881923E-4 82-83 0.0014037344440701153 84-85 0.006635835553785999 86-87 0.004466427776586731 88-89 0.0 90-91 0.001276122221881923 92-93 1.276122221881923E-4 94-95 2.552244443763846E-4 96-97 0.0 98-99 0.0 100-101 2.552244443763846E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 391812.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.67013800421041 #Duplication Level Percentage of deduplicated Percentage of total 1 76.11149179722902 24.86572940721801 2 11.366065545201549 7.426618598532713 3 4.5766007412778045 4.485545334231525 4 2.3409757311186836 3.05920000800619 5 1.299868515038902 2.123344188682449 6 0.9138025114338009 1.7912432494281803 7 0.6048446320887139 1.3832250321010908 8 0.4055395186111261 1.0599225631349236 9 0.32036090360358793 0.9419611438694488 >10 1.768221040579376 10.345401312967837 >50 0.13361275377749554 2.947878290580427 >100 0.11486008658065407 7.836077232936212 >500 0.023440833996051853 5.448265184954802 >1k 0.016408583797236295 12.18310004297207 >5k 7.813611332017283E-4 1.3075019574257836 >10k+ 0.0031254445328069134 12.794986452958327 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16206 4.136167345563688 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12405 3.1660592324890513 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10839 2.766377752595633 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10673 2.7240104948291526 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5122 1.307259604095842 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4192 1.0699008708258042 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4031 1.0288097352812062 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3809 0.9721499086296489 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3594 0.9172766530887262 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3095 0.7899196553449104 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3002 0.7661837820179066 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2547 0.6500566598266515 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2342 0.5977356487294927 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2332 0.5951834042857288 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1965 0.5015160331995957 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1929 0.4923279532020459 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1766 0.45072636876869515 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1758 0.44868457321368416 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1725 0.44026216654926337 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1685 0.430053188774208 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1654 0.4221412309985401 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1450 0.3700754443457577 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1399 0.35705899768256205 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1201 0.3065245576960379 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1162 0.29657080436535893 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1088 0.2776841954815064 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 968 0.2470572621563403 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 954 0.2434841199350709 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 925 0.23608261104815573 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 918 0.23429603993752107 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 862 0.22000347105244353 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 847 0.21617510438679774 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 845 0.215664655498045 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 842 0.21489898216491585 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 826 0.2108153910548937 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 821 0.20953926883301174 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 792 0.20213775994609662 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 733 0.1870795177278899 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 709 0.18095413106285668 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 700 0.1786571110634692 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 691 0.17636009106408174 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 687 0.1753391932865762 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 687 0.1753391932865762 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 665 0.16972425551029577 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 641 0.16359886884526254 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 640 0.16334364440088614 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 636 0.1623227466233806 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 617 0.1574734821802293 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 603 0.1539003399589599 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 553 0.14113911774014068 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 549 0.14011821996263515 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 545 0.1390973221851296 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 543 0.13858687329637684 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 535 0.13654507774136576 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 507 0.12939879329882698 No Hit ACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAA 502 0.12812267107694505 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 465 0.11867936663501884 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 460 0.1174032444131369 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 456 0.11638234663563138 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 454 0.1158718977468786 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 440 0.11229875552560922 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 439 0.11204353108123283 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 436 0.11127785774810367 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 430 0.10974651108184537 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 427 0.10898083774871622 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 417 0.10642859330495238 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACAAAAAAAAAAAAAAAA 415 0.1059181444161996 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 411 0.10489724663869406 No Hit ACGCAGAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAA 394 0.10055843108429552 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.552244443763846E-4 0.0 0.0 0.0 0.0 8 2.552244443763846E-4 0.0 0.0 0.0 0.0 9 2.552244443763846E-4 0.0 0.0 0.0 0.0 10-11 2.552244443763846E-4 0.0 0.0 0.0 0.0 12-13 2.552244443763846E-4 0.0 0.0 0.0 0.0 14-15 2.552244443763846E-4 0.0 0.0 0.0 0.0 16-17 2.552244443763846E-4 0.0 0.0 0.0 0.0 18-19 2.552244443763846E-4 0.0 0.0 1.276122221881923E-4 0.0 20-21 2.552244443763846E-4 0.0 0.0 0.0019141833328228846 0.0 22-23 2.552244443763846E-4 0.0 0.0 0.007018672220350576 0.0 24-25 2.552244443763846E-4 0.0 0.0 0.016334364440088615 0.0 26-27 2.552244443763846E-4 0.0 0.0 0.03368962665768277 0.0 28-29 2.552244443763846E-4 0.0 0.0 0.07159045664757588 0.0 30-31 2.552244443763846E-4 0.0 0.0 0.15977050217961675 0.0 32-33 2.552244443763846E-4 0.0 0.0 0.27921554214776473 0.0 34-35 3.828366665645769E-4 0.0 0.0 0.43043602544077264 0.0 36-37 5.104488887527692E-4 0.0 0.0 0.6440588853838065 0.0 38-39 7.656733331291537E-4 0.0 0.0 0.921105019754372 0.0 40-41 7.656733331291537E-4 0.0 0.0 1.2373281063367125 0.0 42-43 0.0015313466662583075 0.0 0.0 1.5019958551550232 0.0 44-45 0.0017865711106346922 0.0 0.0 1.774575561749002 0.0 46-47 0.0017865711106346922 0.0 0.0 2.103559870550162 0.0 48-49 0.0017865711106346922 0.0 0.0 2.457173338233643 0.0 50-51 0.0017865711106346922 0.0 0.0 2.8052994803630313 0.0 52-53 0.0017865711106346922 0.0 0.0 3.0928098169530287 0.0 54-55 0.0017865711106346922 0.0 0.0 3.3821067246536605 0.0 56-57 0.002041795555011077 0.0 0.0 3.6910559145712742 0.0 58-59 0.002041795555011077 0.0 0.0 4.056920155584821 0.0 60-61 0.0021694077771992693 0.0 0.0 4.517089828795443 0.0 62-63 0.002297019999387461 0.0 0.0 4.8999264953600195 0.0 64-65 0.002297019999387461 0.0 0.0 5.21691525527549 0.0 66-67 0.002552244443763846 0.0 0.0 5.562233928516738 0.0 68-69 0.002552244443763846 0.0 0.0 5.946601941747573 0.0 70-71 0.002552244443763846 0.0 0.0 6.408941022735394 0.0 72-73 0.002552244443763846 0.0 0.0 6.879192061498882 0.0 74-75 0.002552244443763846 0.0 0.0 7.300184782497729 0.0 76-77 0.002552244443763846 0.0 0.0 7.672429634620686 0.0 78-79 0.002552244443763846 0.0 0.0 8.121879881167498 0.0 80-81 0.002552244443763846 0.0 0.0 8.601701836595101 0.0 82-83 0.002552244443763846 0.0 0.0 9.063147632027604 0.0 84-85 0.002552244443763846 0.0 0.0 9.67262360519841 0.0 86-87 0.002552244443763846 0.0 0.0 10.262064459485671 0.0 88-89 0.002552244443763846 0.0 0.0 10.742779700468592 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGGCA 20 0.0020738821 71.24282 4 TATAACG 30 1.2999863E-4 63.335037 2 GGGTATG 25 0.005020814 56.99426 7 TAACGCA 45 9.6220657E-4 42.217968 4 GTGCTAA 45 9.6220657E-4 42.217968 9 ATAACGC 45 9.6220657E-4 42.217968 3 GTAAGCG 30 8.293256E-5 39.579346 94-95 GCGGGAT 50 0.0016153152 37.996174 7 GGACCGA 65 1.3273738E-4 36.534782 6 GTATAAC 55 0.0025739945 34.555202 1 GAGTAAT 55 0.0025788336 34.541973 6 CGGGATC 55 0.0025788336 34.541973 8 GGACTTG 75 3.07659E-4 31.663477 8 ATGGGTG 245 0.0 31.01728 5 ACTACCT 80 4.4909446E-4 29.68451 5 ATAGTGA 80 4.4909446E-4 29.68451 6 ATGGGGA 695 0.0 29.385529 5 TCCTGAG 65 0.0058386656 29.231556 2 CATGGGT 455 0.0 29.227825 4 ATGGGGT 325 0.0 29.227825 5 >>END_MODULE