FastQCFastQC Report
Thu 26 May 2016
SRR938519_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938519_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3209161
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108230.33725325715973736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85840.26748424276625576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58300.18166742023849847No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA58100.18104420438862368No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA55940.17431347320997606No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA55820.17393954370005119No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC55760.17375257894508878No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA49070.15290600876677737No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48030.14966528634742848No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG43640.13598569844267708No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA41990.13084416768121013No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT40960.12763460605435503No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA40280.12551567216478077No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG39590.12336557748271278No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA39080.12177637706553207No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG33930.1057285689312565No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT33440.10420169009906328No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33120.10320454473926363No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT32200.10033775182983963No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98550.037.8366361
GTATCAA151800.034.2140051
TAAGGTA11700.027.2354934
TATCAAC191800.026.9542482
AGGTAAG23700.026.890742
ATCAACG192150.026.88043
TCAACGC194750.026.5459564
CAACGCA195200.026.436035
TAAGGTG26850.026.3929715
AACGCAG200850.025.7661746
GGTAAGG26600.024.4954383
GTACATG125600.024.4243581
GTGTAAG14500.024.2726291
GTAAGGT28000.024.1199594
TACATGG123600.023.8956812
AAGGTAA26550.023.1088451
ACGCAGA222750.022.7820767
CGCAGAG224150.022.4903438
GAGTACT129300.022.43224312-13
ACATGGG127550.022.1861933
Produced by FastQC (version 0.11.5)