##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938519_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3209161 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.228130343102137 31.0 31.0 34.0 28.0 34.0 2 31.27430315898766 31.0 31.0 34.0 28.0 34.0 3 31.307167823614957 31.0 31.0 34.0 28.0 34.0 4 34.98100749697507 37.0 35.0 37.0 32.0 37.0 5 34.977763970084396 37.0 35.0 37.0 32.0 37.0 6 34.996125467061326 37.0 35.0 37.0 32.0 37.0 7 34.919239327662275 37.0 35.0 37.0 32.0 37.0 8 34.88044476422342 37.0 35.0 37.0 32.0 37.0 9 36.390209465963224 39.0 37.0 39.0 32.0 39.0 10-11 36.23666575157806 39.0 35.0 39.0 32.0 39.0 12-13 36.11670869738228 39.0 35.0 39.0 31.0 39.0 14-15 37.10144551800299 39.0 36.0 41.0 31.0 41.0 16-17 36.95963181654021 39.0 36.0 41.0 31.0 41.0 18-19 37.12848747694491 39.0 36.0 41.0 31.0 41.0 20-21 37.115310824231 39.0 36.0 41.0 31.0 41.0 22-23 37.04410062318469 39.0 36.0 41.0 31.0 41.0 24-25 36.91731530452975 39.0 36.0 41.0 30.5 41.0 26-27 36.723671545304214 39.0 36.0 41.0 30.0 41.0 28-29 36.53585578909877 39.0 36.0 41.0 30.0 41.0 30-31 36.26412682317901 39.0 35.0 40.0 29.5 41.0 32-33 35.98522370800343 38.5 35.0 40.0 29.0 41.0 34-35 35.73721028642689 38.0 35.0 40.0 27.5 41.0 36-37 35.45075737864195 38.0 34.5 40.0 26.5 41.0 38-39 35.28879869224386 38.0 34.0 40.0 26.0 41.0 40-41 35.18291447515409 38.0 34.0 40.0 25.5 41.0 42-43 34.89779992340677 38.0 34.0 40.0 24.5 41.0 44-45 34.55657584645956 38.0 33.0 40.0 24.0 41.0 46-47 34.17178789097836 38.0 33.0 40.0 23.0 41.0 48-49 33.809746846605705 38.0 33.0 40.0 21.5 41.0 50-51 33.10011245930011 37.5 32.0 39.5 20.0 40.5 52-53 33.12232449540549 37.0 32.0 39.5 19.5 40.0 54-55 33.248485195974894 37.0 32.0 40.0 18.5 41.0 56-57 32.95478304142422 37.0 32.0 40.0 16.5 41.0 58-59 32.61229913363648 37.0 31.5 40.0 13.5 41.0 60-61 32.17125379499502 36.0 31.0 39.0 10.0 41.0 62-63 31.65509692408701 35.5 30.5 39.0 8.0 40.0 64-65 31.173080752258926 35.0 30.0 39.0 6.5 40.0 66-67 30.674006227795985 35.0 29.0 38.0 2.0 40.0 68-69 30.134164350121416 34.5 28.5 38.0 2.0 40.0 70-71 29.523063193152353 34.0 28.0 37.0 2.0 39.5 72-73 28.92567870543111 34.0 26.5 36.5 2.0 39.0 74-75 28.297696344932525 34.0 26.0 36.0 2.0 39.0 76-77 27.58309243444003 33.0 25.5 35.5 2.0 38.0 78-79 26.831923359407647 32.5 24.5 35.0 2.0 37.0 80-81 26.176364943983803 32.0 22.5 35.0 2.0 36.5 82-83 25.469131184131925 31.5 20.0 35.0 2.0 36.0 84-85 24.785110968256188 31.0 18.0 34.0 2.0 36.0 86-87 24.062878272545376 31.0 10.0 34.0 2.0 35.0 88-89 23.262097632371827 30.0 4.5 34.0 2.0 35.0 90-91 22.374999571539103 29.5 2.0 34.0 2.0 35.0 92-93 21.467041229779372 29.0 2.0 34.0 2.0 35.0 94-95 20.483950478022138 28.0 2.0 34.0 2.0 35.0 96-97 19.257623098373685 26.0 2.0 33.0 2.0 35.0 98-99 17.737841915690737 23.5 2.0 33.0 2.0 34.0 100-101 14.060733007786148 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.48395669030468724 1101 2 0.4387777649017792 1101 3 0.45720152097223377 1101 4 0.27509432141297196 1101 5 0.2804990895786261 1101 6 0.4360655654553227 1101 7 0.37746844403664426 1101 8 0.3948087625580001 1101 9 0.5156716029351571 1101 10-11 0.6664959184530659 1101 12-13 0.5920369792539475 1101 14-15 0.707874018128436 1101 16-17 0.6230588483153667 1101 18-19 0.7324590964349298 1101 20-21 0.7598045128684916 1101 22-23 0.7087981463443143 1101 24-25 0.7276112183871035 1101 26-27 0.7177345568585807 1101 28-29 0.676051439039064 1101 30-31 0.6551001966893395 1101 32-33 0.7882731769702644 1101 34-35 0.6081848257750337 1101 36-37 0.7007664237305349 1101 38-39 0.7261059332983066 1101 40-41 0.7422931947806362 1101 42-43 0.9559362211958771 1101 44-45 0.8357790397251605 1101 46-47 0.6963388536512767 1101 48-49 0.6269392971156691 1101 50-51 0.6633534266390413 1101 52-53 0.6141442826245509 1101 54-55 0.6209405591727162 1101 56-57 0.6971403749093668 1101 58-59 0.7782647486789571 1101 60-61 0.6685731340688505 1101 62-63 0.7040166503913348 1101 64-65 0.5355624906293919 1101 66-67 0.6194648710326902 1101 68-69 0.47227242262320956 1101 70-71 0.6184436823674595 1101 72-73 0.7825726686063987 1101 74-75 0.6806168739 1101 76-77 0.6831171477748086 1101 78-79 0.8836762247365648 1101 80-81 1.00681360917363 1101 82-83 0.9791004128477852 1101 84-85 0.9440609407993072 1101 86-87 1.082007444986072 1101 88-89 1.213047962974823 1101 90-91 1.0330750647701024 1101 92-93 0.9832831135593985 1101 94-95 0.7691134294128048 1101 96-97 0.717560501466469 1101 98-99 0.9471154633933594 1101 100-101 0.6578281638826713 1102 1 0.5270624368676877 1102 2 0.4059594454971531 1102 3 0.49269450849232754 1102 4 0.43582422412644917 1102 5 0.47787926291275085 1102 6 0.4546180265735629 1102 7 0.42928115589649707 1102 8 0.5073789027656375 1102 9 0.7264815057115754 1102 10-11 0.7724041732452349 1102 12-13 0.7298883458118581 1102 14-15 0.8242819451439374 1102 16-17 0.9264353348668166 1102 18-19 0.7810089256356818 1102 20-21 0.8072997391576564 1102 22-23 0.8325243645044438 1102 24-25 0.732371684389939 1102 26-27 0.6595482640474728 1102 28-29 0.5196673474656492 1102 30-31 0.6452944353472176 1102 32-33 0.7153510312401963 1102 34-35 0.5211522845478882 1102 36-37 0.5627745439893559 1102 38-39 0.6457166560475045 1102 40-41 0.8192558649586275 1102 42-43 0.8393980096749516 1102 44-45 0.8942992726400263 1102 46-47 0.8753930392852638 1102 48-49 0.8791103012007184 1102 50-51 0.8095868668358221 1102 52-53 0.724050442373688 1102 54-55 0.6985909112293882 1102 56-57 0.7566771154677383 1102 58-59 0.8625692490742694 1102 60-61 0.7829383416670765 1102 62-63 0.9170110315687872 1102 64-65 1.1492257879958068 1102 66-67 0.7591028466514551 1102 68-69 0.816687938268565 1102 70-71 0.9390063802940176 1102 72-73 1.0473644186269553 1102 74-75 1.0192295819306523 1102 76-77 1.0134957045844288 1102 78-79 1.1554927815301852 1102 80-81 1.3186437045403174 1102 82-83 1.2970974829872723 1102 84-85 1.3466719288384432 1102 86-87 1.4647810926073355 1102 88-89 1.3024336902187343 1102 90-91 1.2847079486055009 1102 92-93 1.3790500670355712 1102 94-95 1.3564957783080231 1102 96-97 1.4239146348161675 1102 98-99 1.5578150013587369 1102 100-101 1.4702042477127186 1103 1 0.3708754355866475 1103 2 0.3960221393898209 1103 3 0.3280102776577962 1103 4 0.24058488532939037 1103 5 0.26316265103764636 1103 6 0.43364363198172384 1103 7 0.32145161166967284 1103 8 0.32393601717967613 1103 9 0.5991278917837164 1103 10-11 0.6044649841603302 1103 12-13 0.6154234972989485 1103 14-15 0.7198921996545877 1103 16-17 0.8147924214992273 1103 18-19 0.8396416987060178 1103 20-21 0.6835053850035351 1103 22-23 0.7574564075351091 1103 24-25 0.8689295396476098 1103 26-27 0.7183953878763916 1103 28-29 0.7969756773262731 1103 30-31 0.8076673575123294 1103 32-33 0.8949297998963317 1103 34-35 0.816372423147385 1103 36-37 0.8524297121454296 1103 38-39 0.8637100193232712 1103 40-41 1.0114348404336155 1103 42-43 0.9130954059182628 1103 44-45 1.030365763356329 1103 46-47 0.6427023878058904 1103 48-49 0.7996553151687067 1103 50-51 1.0044723293229012 1103 52-53 1.0883221467770738 1103 54-55 0.950444100715579 1103 56-57 0.9127075517937655 1103 58-59 0.8415267586049069 1103 60-61 1.02258742873164 1103 62-63 1.0143561566633217 1103 64-65 1.1788663244900768 1103 66-67 1.1124941509992787 1103 68-69 1.2067252582667116 1103 70-71 1.1218815327071603 1103 72-73 1.146051828915418 1103 74-75 1.2355401806249162 1103 76-77 1.2745942522222151 1103 78-79 1.2231254465608217 1103 80-81 1.3572814783051896 1103 82-83 1.4014909944317644 1103 84-85 1.5726809115675273 1103 86-87 1.6521763789897044 1103 88-89 1.6348703558050026 1103 90-91 1.7711644626275103 1103 92-93 1.7845703399129746 1103 94-95 1.7289275666736899 1103 96-97 2.1275568073401274 1103 98-99 2.065437109367565 1103 100-101 1.865147866425195 1104 1 0.4196693287164166 1104 2 0.41210710454083355 1104 3 0.39983503703383505 1104 4 0.3332951408886373 1104 5 0.35817493115713006 1104 6 0.46116968574872885 1104 7 0.43247451243303203 1104 8 0.4690618054305915 1104 9 0.5884488343658631 1104 10-11 0.6209814129688311 1104 12-13 0.6473040150553402 1104 14-15 0.8110598066907713 1104 16-17 0.8423755612270654 1104 18-19 0.7985968928646656 1104 20-21 0.7904353551329777 1104 22-23 0.6915101745447387 1104 24-25 0.7345252913900637 1104 26-27 0.8224936288322482 1104 28-29 0.7899687075287289 1104 30-31 0.7649949379968959 1104 32-33 0.8087087577456558 1104 34-35 0.8324215436253155 1104 36-37 1.088551849550008 1104 38-39 0.9548670057155633 1104 40-41 1.011393685438584 1104 42-43 0.9650688543085693 1104 44-45 0.9379289893769069 1104 46-47 0.7582616310850128 1104 48-49 0.676563380220145 1104 50-51 0.865430842432751 1104 52-53 0.9042185954992732 1104 54-55 0.8207527631782412 1104 56-57 0.8903630446580237 1104 58-59 0.9715141465903088 1104 60-61 1.0466591180778124 1104 62-63 1.1724099236729408 1104 64-65 1.1110203238262848 1104 66-67 1.0856876458549074 1104 68-69 1.2178291414396298 1104 70-71 1.282169617672313 1104 72-73 1.2715387190581353 1104 74-75 1.1548432008705198 1104 76-77 1.2888830009776093 1104 78-79 1.3943886393918845 1104 80-81 1.3259704925766833 1104 82-83 1.3932998228419855 1104 84-85 1.397950078510771 1104 86-87 1.5880529140046384 1104 88-89 1.5504455300499416 1104 90-91 1.4667595505797166 1104 92-93 1.4116025337397247 1104 94-95 1.4153517286385089 1104 96-97 1.6291366281167612 1104 98-99 1.6244175275554191 1104 100-101 1.5488332298503522 1105 1 0.3559395978160431 1105 2 0.1910074509770361 1105 3 0.22085960612760402 1105 4 0.16665669224175161 1105 5 0.2134936224853874 1105 6 0.3137019943145347 1105 7 0.21148378362612874 1105 8 0.2339381694304521 1105 9 0.29757161881123295 1105 10-11 0.3569894625190244 1105 12-13 0.3976833047144339 1105 14-15 0.4880532550960126 1105 16-17 0.44406370292697517 1105 18-19 0.578337378831236 1105 20-21 0.4612796222821913 1105 22-23 0.5460204649470981 1105 24-25 0.5914951902841992 1105 26-27 0.5514420525864665 1105 28-29 0.5871302201127335 1105 30-31 0.6135478656671225 1105 32-33 0.7566336557793889 1105 34-35 0.665649686155227 1105 36-37 0.7480849047998248 1105 38-39 0.6747121575983996 1105 40-41 0.8460533190438682 1105 42-43 0.7409471521762825 1105 44-45 0.8478301324254076 1105 46-47 0.8458606061894685 1105 48-49 0.8083417993133253 1105 50-51 0.7175382804360666 1105 52-53 0.6656351315575861 1105 54-55 0.7509407954733192 1105 56-57 0.7830170566363321 1105 58-59 0.7672225645110231 1105 60-61 0.8229209996511031 1105 62-63 0.8460612703552677 1105 64-65 0.9150825558151077 1105 66-67 1.0389164114216278 1105 68-69 1.09721113586793 1105 70-71 1.0904612987547075 1105 72-73 0.9587773889897733 1105 74-75 1.0860588171600725 1105 76-77 1.2735769922890192 1105 78-79 1.3112111452736634 1105 80-81 1.4583458641450555 1105 82-83 1.435248069606807 1105 84-85 1.3804236477211411 1105 86-87 1.4679559549683496 1105 88-89 1.3651593443868926 1105 90-91 1.5159140608274484 1105 92-93 1.5277549443320773 1105 94-95 1.5167334727852442 1105 96-97 1.7910797903038045 1105 98-99 1.930849433099624 1105 100-101 1.8113690451318867 1106 1 0.3037327534066101 1106 2 0.18571840393360617 1106 3 0.15801406321700284 1106 4 0.1470028556016203 1106 5 0.3012310137832941 1106 6 0.15291858605397834 1106 7 0.32060609588723565 1106 8 0.2758991636474164 1106 9 0.115601977404161 1106 10-11 0.18114282193798203 1106 12-13 0.21466671947787574 1106 14-15 0.5332453700604276 1106 16-17 0.287197253654206 1106 18-19 0.1663970847773868 1106 20-21 0.40325876695532514 1106 22-23 0.39642423028186613 1106 24-25 0.15768503705895398 1106 26-27 0.15330870970459642 1106 28-29 0.41434682913833143 1106 30-31 0.08708215459522961 1106 32-33 0.36280075686342883 1106 34-35 0.018413062238856526 1106 36-37 0.15802098309126222 1106 38-39 0.245386576691196 1106 40-41 0.492654625547452 1106 42-43 0.12489165376104694 1106 44-45 0.4605173527344917 1106 46-47 0.45115197863147927 1106 48-49 0.725334433356096 1106 50-51 0.44119743092063146 1106 52-53 0.46939412807195424 1106 54-55 0.5927221166371979 1106 56-57 -0.26070023087059013 1106 58-59 0.15608039663865014 1106 60-61 0.6480663576410706 1106 62-63 0.6273031031644614 1106 64-65 0.6379255016876506 1106 66-67 0.21236789146579582 1106 68-69 0.4106224914682919 1106 70-71 0.38672582374985964 1106 72-73 0.7558061572570125 1106 74-75 1.094402007713935 1106 76-77 0.8702200942307883 1106 78-79 1.1151697042015272 1106 80-81 1.2266537991870017 1106 82-83 0.995347532871893 1106 84-85 0.9874048440954972 1106 86-87 0.9876615986078079 1106 88-89 1.0519751267230006 1106 90-91 1.12271503881189 1106 92-93 0.9842000231507413 1106 94-95 0.9477090062995899 1106 96-97 0.993267434740897 1106 98-99 1.0251477924383217 1106 100-101 0.6683151547400641 1107 1 0.18159653909312823 1107 2 0.06867538146759955 1107 3 0.1441632493975895 1107 4 0.0770848919230005 1107 5 0.13930122233434616 1107 6 0.13889388583568518 1107 7 0.1147069294157177 1107 8 0.05153512572367447 1107 9 0.18639811108716486 1107 10-11 0.3112216931579752 1107 12-13 0.3454715251503586 1107 14-15 0.42404427466349404 1107 16-17 0.4893858727262952 1107 18-19 0.39599970532600537 1107 20-21 0.480496852872875 1107 22-23 0.48147492239051104 1107 24-25 0.410620668605425 1107 26-27 0.37186265060535817 1107 28-29 0.45441730410160375 1107 30-31 0.4598470201832683 1107 32-33 0.5854612711943545 1107 34-35 0.5184318975048328 1107 36-37 0.4120007582875118 1107 38-39 0.5530715205562728 1107 40-41 0.45750059190728365 1107 42-43 0.49827497627866535 1107 44-45 0.4353802824843882 1107 46-47 0.4957527234206154 1107 48-49 0.5382106289585806 1107 50-51 0.4927833894434315 1107 52-53 0.554109911675333 1107 54-55 0.6342921312044325 1107 56-57 0.6812219060815963 1107 58-59 0.5744199553186533 1107 60-61 0.5412491370039874 1107 62-63 0.6305974300211794 1107 64-65 0.6022810229643163 1107 66-67 0.7624392130451447 1107 68-69 0.8867647144258015 1107 70-71 0.8960922908403752 1107 72-73 0.9102419886126398 1107 74-75 0.836459593529927 1107 76-77 0.9608877733415468 1107 78-79 1.0978441236190157 1107 80-81 1.2123199190484222 1107 82-83 0.9028207935904184 1107 84-85 1.0722395617237943 1107 86-87 1.1838804577965227 1107 88-89 1.2551158490729257 1107 90-91 0.9960755939590236 1107 92-93 1.1104829086513561 1107 94-95 1.191376327734467 1107 96-97 1.1783423699728246 1107 98-99 1.1523184112098193 1107 100-101 1.0264371544472422 1108 1 0.2702249841017057 1108 2 0.2024448128070162 1108 3 0.2704991340699294 1108 4 0.21294241964746874 1108 5 0.37812668882667566 1108 6 0.3245407560487408 1108 7 0.38233245096191837 1108 8 0.3796357927420715 1108 9 0.3833928325426257 1108 10-11 0.45396923803579625 1108 12-13 0.4479099527506918 1108 14-15 0.5710553083763372 1108 16-17 0.6303291927294552 1108 18-19 0.6279096667628039 1108 20-21 0.4442113195512647 1108 22-23 0.48231793475469686 1108 24-25 0.5705499269329621 1108 26-27 0.6471606679014457 1108 28-29 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1.8632966882353585 1108 96-97 2.0170045864517725 1108 98-99 1.8062987931715213 1108 100-101 1.389608537650206 1109 1 0.290444251549264 1109 2 0.30041927127477663 1109 3 0.28738111848815606 1109 4 0.2857325610282899 1109 5 0.22973428677629215 1109 6 0.25382420506763026 1109 7 0.33794026542803834 1109 8 0.34181436398527154 1109 9 0.5024680104828576 1109 10-11 0.3891008570824255 1109 12-13 0.3529746963751421 1109 14-15 0.3097708687203067 1109 16-17 0.35076180678805 1109 18-19 0.3328988891348885 1109 20-21 0.20211778664705804 1109 22-23 0.3679901777099346 1109 24-25 0.31507074328914797 1109 26-27 0.416265839309105 1109 28-29 0.3101913499099922 1109 30-31 0.3325168888924068 1109 32-33 0.3967031436135997 1109 34-35 0.3077759733796839 1109 36-37 0.16691702315329593 1109 38-39 0.22487214301744274 1109 40-41 0.26685616868240203 1109 42-43 0.24573912050320956 1109 44-45 0.12584907296773196 1109 46-47 0.35359665963229503 1109 48-49 0.5396216389864179 1109 50-51 0.4577226448692642 1109 52-53 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-1.4816289774585485 2316 56-57 -1.5405673045436465 2316 58-59 -1.3873258923763316 2316 60-61 -1.3530346031451899 2316 62-63 -1.5056280133464384 2316 64-65 -1.6780967027244955 2316 66-67 -1.722567976291188 2316 68-69 -1.9289490342628355 2316 70-71 -2.3041232379748315 2316 72-73 -1.9358865859170642 2316 74-75 -1.8881278232783707 2316 76-77 -2.279006311585654 2316 78-79 -2.431023311977615 2316 80-81 -2.371092152834354 2316 82-83 -2.575390714339786 2316 84-85 -2.6321867020072354 2316 86-87 -2.724326277412768 2316 88-89 -3.0351352822737105 2316 90-91 -2.815478928893686 2316 92-93 -3.025658652822578 2316 94-95 -3.563394237511499 2316 96-97 -3.6200242400414187 2316 98-99 -3.5698856706389392 2316 100-101 -3.163826003248447 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9265.0 3 2861.0 4 3011.0 5 3390.0 6 4221.0 7 5430.0 8 7088.0 9 9289.0 10 11893.0 11 15366.0 12 19714.0 13 24048.0 14 27961.0 15 32361.0 16 37378.0 17 42898.0 18 40547.0 19 37468.0 20 38712.0 21 41480.0 22 45927.0 23 51570.0 24 57331.0 25 64975.0 26 73089.0 27 83111.0 28 95539.0 29 110339.0 30 126822.0 31 148728.0 32 176153.0 33 212925.0 34 260900.0 35 321544.0 36 384095.0 37 385137.0 38 186609.0 39 9986.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.868397245541864 19.196567553998918 15.269311326255325 22.6657238742039 2 15.130376138223568 20.50766073474552 40.60010050649409 23.761862620536828 3 18.582700648475335 24.894939009876403 30.645521052909057 25.876839288739202 4 12.484650901603544 17.120078617333252 38.12872028451987 32.26655019654333 5 13.010558852682907 38.055063829999725 34.7294871378743 14.204890179443069 6 30.978693301884764 37.41954741630046 17.532308135695885 14.069451146118892 7 27.606440856632315 33.686580343487 21.221033615297173 17.48594518458351 8 26.351138433189032 34.504644652812864 20.33071586219923 18.813501051798873 9 26.43836470342562 16.89316091470389 20.5445897889025 36.12388459296799 10-11 24.368843245221715 26.911038444696946 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21.465549778770203 36-37 22.309669552600045 26.327450631022202 29.418671410615488 21.944208405762264 38-39 21.455302621776546 26.93483431157715 29.349854899894957 22.260008166751348 40-41 22.045413631570867 26.863011745012205 29.45240078772864 21.639173835688286 42-43 21.796236733346518 26.832425706195007 29.062599699104496 22.308737861353976 44-45 21.648572135080972 27.04929003692659 29.51493712321021 21.787200704782226 46-47 22.39400685659934 27.40084210795318 28.45462236112784 21.750528674319632 48-49 21.869587275785836 27.644681592359188 28.176098531759113 22.309632600095856 50-51 22.154715656987147 27.634277850457444 28.386465667738474 21.824540824816932 52-53 22.545368420478564 27.16173229340258 28.464840009754223 21.828059276364637 54-55 21.6370811782629 27.60063431644487 28.674132470043006 22.08815203524922 56-57 21.99527379793889 27.687113892431764 28.274468769746075 22.04314353988327 58-59 21.98930450950017 27.000932041388808 28.343860159819933 22.66590328929109 60-61 21.264634987701676 27.507005516362 28.857163829109815 22.37119566682651 62-63 21.55416718282656 27.472312404120864 28.971209574941355 22.00231083811122 64-65 21.203169264325116 27.54875644441439 28.648515909038142 22.59955838222235 66-67 21.12296973780409 27.547539252722654 28.541954025495958 22.787536983977297 68-69 21.920046920864984 27.037836803522307 28.743657689602397 22.298458586010312 70-71 21.739487539465657 27.03303660125137 28.21277495044681 23.01470090883616 72-73 21.51006952501964 27.2841012888724 28.354740772244973 22.85108841386299 74-75 21.62892927675873 27.813753029713634 28.14619552749434 22.411122166033294 76-77 21.775087621419363 27.152603863913686 28.390664520221108 22.68164399444584 78-79 21.75445687284016 27.50379440103621 28.15384023300684 22.58790849311679 80-81 22.181706111056318 27.490630681753554 27.752315627413616 22.575347579776516 82-83 22.044031159458115 27.45070665032709 27.984085273268512 22.52117691694629 84-85 21.749473628774325 27.707249564856586 28.09054660183026 22.452730204538828 86-87 22.134039364092377 27.68262523270452 27.51866661534844 22.66466878785466 88-89 21.800676695104404 27.675031110268755 27.389256171554116 23.135036023072722 90-91 21.79833344139489 27.540922080966368 27.607411111055697 23.053333366583043 92-93 22.116031838724854 27.02984494728638 27.819703783844773 23.03441943014399 94-95 21.580021202500717 26.75485298305717 28.181742935344534 23.48338287909758 96-97 21.998404498578296 26.212735393352826 28.224086584788367 23.56477352328051 98-99 21.6699417314316 26.707058352880086 27.5846011644363 24.038398751252014 100-101 22.07407305770042 25.969649049989197 28.140739246209485 23.8155386461009 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 448.0 1 333.5 2 297.5 3 394.5 4 381.5 5 376.5 6 594.0 7 1452.0 8 2453.0 9 2855.5 10 2539.0 11 2151.5 12 2246.5 13 2396.0 14 2678.5 15 3039.0 16 3371.0 17 3767.0 18 4124.0 19 4664.0 20 5388.5 21 6403.0 22 7754.5 23 9506.0 24 11777.0 25 14813.5 26 19080.0 27 24172.0 28 29468.0 29 35569.0 30 42344.5 31 52151.5 32 66858.0 33 73259.5 34 72135.5 35 76394.0 36 84401.5 37 91528.0 38 96058.0 39 99014.5 40 102005.5 41 106759.0 42 109175.0 43 110172.0 44 111984.0 45 116801.0 46 118720.0 47 120185.0 48 122630.5 49 117120.0 50 110148.5 51 104094.0 52 99062.0 53 99059.5 54 100392.0 55 94619.0 56 85618.5 57 76962.0 58 70993.0 59 61734.0 60 47310.5 61 38872.5 62 34348.5 63 25866.5 64 17053.5 65 12270.0 66 8713.0 67 6390.0 68 5019.0 69 4084.5 70 3284.5 71 2347.0 72 1729.5 73 1412.0 74 1117.5 75 867.0 76 625.5 77 425.5 78 290.5 79 194.0 80 118.5 81 60.0 82 32.0 83 18.5 84 14.0 85 9.0 86 7.0 87 9.0 88 5.5 89 2.0 90 2.5 91 3.5 92 3.0 93 2.0 94 1.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00791484129340971 2 0.10588437289372518 3 0.1134876062621975 4 0.11719574056895245 5 0.15658298228103856 6 0.09597524088071617 7 0.025863457769803384 8 0.02642435203469069 9 0.02153210761317366 10-11 0.051649013558372425 12-13 0.05384584942918102 14-15 0.027109889469552947 16-17 0.03064663941759232 18-19 0.03552330344286248 20-21 0.05785001126462649 22-23 0.02986761960524885 24-25 0.04737998498673018 26-27 0.056432195206161356 28-29 0.05261499812567834 30-31 0.06799284922133854 32-33 0.0490003461964046 34-35 0.06155814557138142 36-37 0.05317589239056563 38-39 0.03212677706104493 40-41 0.03750201376621491 42-43 0.033357628364547615 44-45 0.007057919499831887 46-47 0.013321238791073432 48-49 0.008943147445703098 50-51 0.00794600208590345 52-53 0.009145692596912402 54-55 0.010984179354043003 56-57 0.014131419395910646 58-59 0.01908598540241515 60-61 0.024819571221263124 62-63 0.011482752033942828 64-65 0.008647119917012579 66-67 0.010563508655377528 68-69 0.011030920542783613 70-71 0.030911506153789105 72-73 0.027779846508168334 74-75 0.02919766256663346 76-77 0.023557559125266695 78-79 0.004876664025270157 80-81 0.004658538477813983 82-83 0.0057180054226011095 84-85 0.005032467987738851 86-87 0.013227756413592213 88-89 0.03792268446488038 90-91 0.03468196204553153 92-93 0.028963956622930418 94-95 0.04737998498673018 96-97 0.04337582315128471 98-99 0.025785555788569038 100-101 0.07503518832492356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3209161.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.36900785494117 #Duplication Level Percentage of deduplicated Percentage of total 1 69.15795921003861 29.993120762118654 2 14.64764239116095 12.70507435837257 3 6.23940797435531 8.117908003499942 4 3.2179577893382243 5.582385465707143 5 1.8481440782352903 4.007608752302465 6 1.16814165932006 3.0396686879241415 7 0.7432927954872962 2.2565109760206816 8 0.551192236634682 1.912372835215369 9 0.3994881503586505 1.5592864257774206 >10 1.8229959762076766 13.80448612710394 >50 0.12452725706566894 3.722953673055726 >100 0.0678470215672866 5.532204795808834 >500 0.006206946708120478 1.7808112365481121 >1k 0.004691296929758345 4.489448508627772 >5k 4.330427935161549E-4 1.1573880138816726 >10k+ 7.217379891935915E-5 0.3387713780355187 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10823 0.33725325715973736 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8584 0.26748424276625576 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5830 0.18166742023849847 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5810 0.18104420438862368 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5594 0.17431347320997606 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5582 0.17393954370005119 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5576 0.17375257894508878 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4907 0.15290600876677737 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4803 0.14966528634742848 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4364 0.13598569844267708 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4199 0.13084416768121013 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4096 0.12763460605435503 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 4028 0.12551567216478077 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3959 0.12336557748271278 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3908 0.12177637706553207 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3393 0.1057285689312565 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3344 0.10420169009906328 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3312 0.10320454473926363 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3220 0.10033775182983963 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.1160792493739015E-5 2 0.0 0.0 0.0 3.1160792493739015E-5 3.1160792493739015E-5 3 0.0 0.0 0.0 3.1160792493739015E-5 3.1160792493739015E-5 4 0.0 0.0 0.0 9.348237748121706E-5 3.1160792493739015E-5 5 0.0 0.0 0.0 9.348237748121706E-5 3.1160792493739015E-5 6 0.0 0.0 0.0 9.348237748121706E-5 3.1160792493739015E-5 7 0.0 0.0 0.0 9.348237748121706E-5 3.1160792493739015E-5 8 0.0 0.0 0.0 9.348237748121706E-5 3.1160792493739015E-5 9 0.0 0.0 0.0 9.348237748121706E-5 3.1160792493739015E-5 10-11 0.0 0.0 0.0 1.2464316997495606E-4 6.232158498747803E-5 12-13 0.0 0.0 0.0 1.2464316997495606E-4 1.2464316997495606E-4 14-15 0.0 0.0 0.0 1.558039624686951E-4 1.8696475496243412E-4 16-17 0.0 0.0 0.0 2.804471324436512E-4 2.1812554745617312E-4 18-19 0.0 0.0 0.0 4.2067069866547676E-4 2.1812554745617312E-4 20-21 0.0 0.0 0.0 4.3625109491234623E-4 2.6486673619678165E-4 22-23 0.0 0.0 0.0 6.387962461216498E-4 3.116079249373902E-4 24-25 0.0 0.0 0.0 0.0011996905110089522 3.116079249373902E-4 26-27 0.0 0.0 0.0 0.0020410319083399055 3.116079249373902E-4 28-29 0.0 0.0 0.0 0.005577781856379284 3.116079249373902E-4 30-31 0.0 0.0 0.0 0.016390576851706723 3.116079249373902E-4 32-33 0.0 0.0 0.0 0.04015068112818272 3.116079249373902E-4 34-35 0.0 0.0 0.0 0.07612581606220442 3.116079249373902E-4 36-37 0.0 0.0 0.0 0.1348327491204087 3.116079249373902E-4 38-39 0.0 0.0 0.0 0.22470047467235205 3.7392950992486823E-4 40-41 0.0 0.0 0.0 0.32133009219543673 3.7392950992486823E-4 42-43 0.0 0.0 0.0 0.42001632202310824 3.7392950992486823E-4 44-45 0.0 0.0 0.0 0.5477755712474381 3.7392950992486823E-4 46-47 0.0 0.0 0.0 0.6981731362184695 3.7392950992486823E-4 48-49 0.0 0.0 0.0 0.850829858645297 3.7392950992486823E-4 50-51 0.0 0.0 0.0 1.023226943116908 3.7392950992486823E-4 52-53 0.0 0.0 0.0 1.1866964605390629 4.0509030241860723E-4 54-55 0.0 0.0 0.0 1.3688468730612144 4.0509030241860723E-4 56-57 0.0 0.0 0.0 1.5836226353243106 4.0509030241860723E-4 58-59 0.0 0.0 0.0 1.806827391956963 4.0509030241860723E-4 60-61 0.0 0.0 0.0 2.049102553595784 4.2067069866547676E-4 62-63 0.0 0.0 0.0 2.2907077581959894 4.674118874060853E-4 64-65 0.0 1.5580396246869507E-5 0.0 2.542564863526635 4.674118874060853E-4 66-67 0.0 3.1160792493739015E-5 0.0 2.845463346962025 4.674118874060853E-4 68-69 0.0 3.1160792493739015E-5 0.0 3.1782294500026644 4.674118874060853E-4 70-71 0.0 3.1160792493739015E-5 0.0 3.530486628748137 4.8299228365295476E-4 72-73 0.0 3.1160792493739015E-5 0.0 3.8930736102052843 5.141530761466938E-4 74-75 0.0 3.1160792493739015E-5 0.0 4.265787849222897 5.297334723935633E-4 76-77 0.0 3.1160792493739015E-5 0.0 4.665238048200137 5.297334723935633E-4 78-79 0.0 3.1160792493739015E-5 0.0 5.11663328826444 5.297334723935633E-4 80-81 0.0 3.1160792493739015E-5 0.0 5.580103335420068 5.297334723935633E-4 82-83 0.0 3.1160792493739015E-5 0.0 6.062830752336826 5.297334723935633E-4 84-85 0.0 3.1160792493739015E-5 0.0 6.564472770297284 5.297334723935633E-4 86-87 0.0 3.1160792493739015E-5 0.0 7.0697450205832615 5.297334723935633E-4 88-89 0.0 3.1160792493739015E-5 0.0 7.602859438962396 5.297334723935633E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9855 0.0 37.836636 1 GTATCAA 15180 0.0 34.214005 1 TAAGGTA 1170 0.0 27.235493 4 TATCAAC 19180 0.0 26.954248 2 AGGTAAG 2370 0.0 26.89074 2 ATCAACG 19215 0.0 26.8804 3 TCAACGC 19475 0.0 26.545956 4 CAACGCA 19520 0.0 26.43603 5 TAAGGTG 2685 0.0 26.392971 5 AACGCAG 20085 0.0 25.766174 6 GGTAAGG 2660 0.0 24.495438 3 GTACATG 12560 0.0 24.424358 1 GTGTAAG 1450 0.0 24.272629 1 GTAAGGT 2800 0.0 24.119959 4 TACATGG 12360 0.0 23.895681 2 AAGGTAA 2655 0.0 23.108845 1 ACGCAGA 22275 0.0 22.782076 7 CGCAGAG 22415 0.0 22.490343 8 GAGTACT 12930 0.0 22.432243 12-13 ACATGGG 12755 0.0 22.186193 3 >>END_MODULE