FastQCFastQC Report
Thu 26 May 2016
SRR938519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938519_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3209161
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103030.3210496450629931No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86270.26882415684348654No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC61860.19276066236626957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61570.19185699938395112No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA59510.1854378761302409No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA56280.17537294015476318No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA53440.1665232750865413No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA49280.15356038540914588No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG48720.1518153810294965No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48720.1518153810294965No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG42330.13190363462599727No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA39220.12221262816044443No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT39190.1221191457829632No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38860.12109083963066983No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA38090.11869145860865192No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT35910.1118984058450168No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT35300.10999759750289873No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA34530.10759821648088083No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34070.10616482002616884No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT33350.10392124296661961No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93250.036.8024641
GTATCAA143000.034.0370941
TAAGGTG27800.030.7594995
GGTAAGG28600.027.075293
GTAAGGT29500.026.4103034
ATCAACG182400.026.4097733
TATCAAC186000.026.0916372
AGGTAAG27550.026.052172
TCAACGC185150.026.0175134
CAACGCA185450.026.001045
TAAGGTA13350.025.2655434
AACGCAG191650.025.1473466
TACATGG116600.024.0922572
AAGGTAA30150.023.9632021
GTACATG120200.023.8847681
GTGTAAG14450.023.0260121
ACATGGG120250.022.7951413
ACGCAGA213100.022.504687
CGCAGAG214300.022.3565068
GAGTACT124450.021.6142412-13
Produced by FastQC (version 0.11.5)