##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938519_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3209161 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.845746598565793 31.0 31.0 34.0 30.0 34.0 2 32.06697638417019 33.0 31.0 34.0 30.0 34.0 3 32.12626882851936 33.0 31.0 34.0 30.0 34.0 4 35.79947157528089 37.0 35.0 37.0 35.0 37.0 5 35.69723644279611 37.0 35.0 37.0 33.0 37.0 6 35.697211202554186 37.0 35.0 37.0 33.0 37.0 7 35.639443455781745 37.0 35.0 37.0 33.0 37.0 8 35.61150406601601 37.0 35.0 37.0 33.0 37.0 9 37.20386387594764 39.0 37.0 39.0 33.0 39.0 10-11 37.11161686808484 39.0 37.0 39.0 33.0 39.0 12-13 37.015609687391816 39.0 37.0 39.0 33.0 39.0 14-15 38.07038490745712 40.0 37.0 41.0 33.0 41.0 16-17 37.998197659762155 40.0 37.0 41.0 33.0 41.0 18-19 38.24083163792655 40.0 37.5 41.0 33.0 41.0 20-21 38.212895208436095 40.0 38.0 41.0 33.0 41.0 22-23 38.118718568498124 40.0 37.0 41.0 33.0 41.0 24-25 37.99940140117619 40.0 37.0 41.0 33.0 41.0 26-27 37.81517973077699 40.0 37.0 41.0 32.0 41.0 28-29 37.71082254832338 40.0 37.0 41.0 32.0 41.0 30-31 37.45010954576601 39.0 36.5 41.0 32.0 41.0 32-33 37.231742034756124 39.0 36.0 41.0 31.0 41.0 34-35 37.124128861094846 39.0 36.0 41.0 31.0 41.0 36-37 37.06063173521054 39.0 36.0 41.0 31.0 41.0 38-39 36.890946107097776 39.0 36.0 41.0 30.5 41.0 40-41 36.72227865787974 39.0 35.0 41.0 30.0 41.0 42-43 36.51237582034682 39.0 35.0 41.0 30.0 41.0 44-45 36.218602930797175 39.0 35.0 40.0 30.0 41.0 46-47 35.849857174507605 38.5 35.0 40.0 28.5 41.0 48-49 35.494326710314624 38.0 35.0 40.0 27.5 41.0 50-51 35.128773221412075 38.0 34.0 40.0 26.5 41.0 52-53 34.67128713704298 38.0 34.0 40.0 25.5 41.0 54-55 34.35958323686471 38.0 33.0 40.0 24.0 41.0 56-57 34.045937240294265 37.5 33.0 40.0 24.0 41.0 58-59 33.59358224782116 37.0 32.5 40.0 23.0 41.0 60-61 33.225124728862156 36.5 32.0 39.0 22.0 41.0 62-63 32.787461271030026 36.0 31.5 39.0 20.5 40.0 64-65 32.299881807114076 35.0 31.0 39.0 19.5 40.0 66-67 31.831604584500433 35.0 31.0 38.0 18.0 40.0 68-69 31.37464200144524 35.0 30.0 38.0 16.0 40.0 70-71 30.83217108770797 34.0 29.5 37.0 12.0 39.5 72-73 30.375861634863444 34.0 29.0 37.0 8.0 39.0 74-75 29.828941738977882 34.0 29.0 36.0 7.0 39.0 76-77 28.570655850547855 32.5 27.0 35.0 7.0 37.0 78-79 29.27442733474575 34.0 29.0 35.0 3.5 37.0 80-81 28.943157884568585 34.0 28.5 35.0 2.0 37.0 82-83 28.524154132497557 33.0 27.5 35.0 2.0 36.5 84-85 28.004959551733304 33.0 26.5 35.0 2.0 36.0 86-87 27.462730445745787 33.0 26.0 35.0 2.0 35.5 88-89 26.756640754390325 32.0 25.0 34.5 2.0 35.0 90-91 26.207953106746594 32.0 24.5 34.0 2.0 35.0 92-93 25.464126137641582 31.0 21.5 34.0 2.0 35.0 94-95 24.730488591878064 31.0 19.0 34.0 2.0 35.0 96-97 23.761378129673147 31.0 6.0 34.0 2.0 35.0 98-99 22.5060841447344 30.5 2.0 34.0 2.0 35.0 100-101 18.535415019688948 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2540927490609768 1101 2 0.24643125117083997 1101 3 0.27598035663419296 1101 4 0.18025111687281026 1101 5 0.11857056057102966 1101 6 0.19652596447811277 1101 7 0.11728990260825611 1101 8 0.1719902974578673 1101 9 0.2656128281310046 1101 10-11 0.24515809173567504 1101 12-13 0.3055763736652466 1101 14-15 0.40682063952154834 1101 16-17 0.41840298586063085 1101 18-19 0.36121837004413493 1101 20-21 0.2780001372386849 1101 22-23 0.31750559380781596 1101 24-25 0.35755163222055586 1101 26-27 0.2945890694248732 1101 28-29 0.2726769821737207 1101 30-31 0.38783985267897236 1101 32-33 0.36403889955759894 1101 34-35 0.45743185567780387 1101 36-37 0.47403570254352445 1101 38-39 0.5030069919285083 1101 40-41 0.5772987671194727 1101 42-43 0.44194154421307275 1101 44-45 0.5479646903202848 1101 46-47 0.3831589200135497 1101 48-49 0.29328416899655707 1101 50-51 0.45117146495343263 1101 52-53 0.5467292520261182 1101 54-55 0.6381026314546929 1101 56-57 0.4723333425252534 1101 58-59 0.5728747373615164 1101 60-61 0.47329833560095835 1101 62-63 0.5715469943625422 1101 64-65 0.39328093996314095 1101 66-67 0.3236695150042941 1101 68-69 0.4536318878846366 1101 70-71 0.5815151857989775 1101 72-73 0.5464623951159346 1101 74-75 0.4334861573152331 1101 76-77 0.29887787849217773 1101 78-79 0.6047734174894615 1101 80-81 0.6428051549289471 1101 82-83 0.5359140293445108 1101 84-85 0.5471058421536341 1101 86-87 0.6293548482436968 1101 88-89 0.9027538649503626 1101 90-91 0.8224590082091865 1101 92-93 1.0100443771339158 1101 94-95 1.0413403617260073 1101 96-97 0.942148517834724 1101 98-99 1.0694890068478067 1101 100-101 1.368581947971272 1102 1 0.2845195249069725 1102 2 0.28232158348353664 1102 3 0.30136662363756983 1102 4 0.1880376323019206 1102 5 0.18693943659276613 1102 6 0.208963643307861 1102 7 0.1764397237571984 1102 8 0.23998346627472245 1102 9 0.2125738551636971 1102 10-11 0.3166632377441516 1102 12-13 0.3411032600727637 1102 14-15 0.48727456726170715 1102 16-17 0.4728424353007412 1102 18-19 0.3856812365996234 1102 20-21 0.3264101745091068 1102 22-23 0.39628490339566724 1102 24-25 0.37764003422438464 1102 26-27 0.3740910088739824 1102 28-29 0.43102465204813 1102 30-31 0.4312156756777412 1102 32-33 0.4625267075637467 1102 34-35 0.4078854516416186 1102 36-37 0.4719111537036227 1102 38-39 0.5084361905286485 1102 40-41 0.4553899096221343 1102 42-43 0.3159482314079085 1102 44-45 0.42604232572274015 1102 46-47 0.5360975863696851 1102 48-49 0.548663437223837 1102 50-51 0.6577021741325808 1102 52-53 0.4930259395474863 1102 54-55 0.4493897545066048 1102 56-57 0.5407688360897751 1102 58-59 0.6827174924217942 1102 60-61 0.5639780561587173 1102 62-63 0.6346641655718557 1102 64-65 0.6927538745537589 1102 66-67 0.7290206084696287 1102 68-69 0.7198618162353725 1102 70-71 0.7372242502041075 1102 72-73 0.7300916191828577 1102 74-75 0.7312660490197622 1102 76-77 0.7091211649716094 1102 78-79 0.8189120992148382 1102 80-81 0.9015121530100174 1102 82-83 0.9407746052531678 1102 84-85 1.0275455284269697 1102 86-87 0.8445617711177604 1102 88-89 0.7880196381289082 1102 90-91 0.876088228342006 1102 92-93 1.2336426843664192 1102 94-95 1.2039067055205237 1102 96-97 1.2044945647589138 1102 98-99 1.4547237005821785 1102 100-101 1.7374544945802768 1103 1 0.25851220907606987 1103 2 0.253961101317806 1103 3 0.20189404256920795 1103 4 0.1758780422353965 1103 5 0.048613187576698635 1103 6 0.17820566766309298 1103 7 0.2510670022372352 1103 8 0.20290942821387858 1103 9 0.3160127895836027 1103 10-11 0.27733592376535654 1103 12-13 0.33977498917094096 1103 14-15 0.4951409684594026 1103 16-17 0.3281130130044332 1103 18-19 0.4715557562088293 1103 20-21 0.4347727557318066 1103 22-23 0.41506695645858827 1103 24-25 0.36797362993983995 1103 26-27 0.358332215214368 1103 28-29 0.4295717959415626 1103 30-31 0.26886078070775454 1103 32-33 0.2748308238761936 1103 34-35 0.4446567316033452 1103 36-37 0.49501133737110337 1103 38-39 0.515751342324414 1103 40-41 0.4968178239929557 1103 42-43 0.6515570659682623 1103 44-45 0.468892783348025 1103 46-47 0.5766961262903507 1103 48-49 0.648983049515671 1103 50-51 0.8002588872563194 1103 52-53 0.7099658849102681 1103 54-55 0.6364441293426211 1103 56-57 0.5819001660584391 1103 58-59 0.7045355844407482 1103 60-61 0.7884089184698411 1103 62-63 0.7646713579288189 1103 64-65 0.8273563965089679 1103 66-67 0.8253069445348267 1103 68-69 0.8043762704033881 1103 70-71 0.6352803999779937 1103 72-73 0.6475932474954362 1103 74-75 0.5593426645990895 1103 76-77 0.6024323347328391 1103 78-79 0.797144693247958 1103 80-81 0.7392498634945 1103 82-83 0.9874482481974027 1103 84-85 0.9873776656065161 1103 86-87 1.050363441102789 1103 88-89 1.0311944617926905 1103 90-91 1.062905965373382 1103 92-93 1.282873596067116 1103 94-95 0.9635522118408453 1103 96-97 1.1922760776233545 1103 98-99 1.472722620185209 1103 100-101 1.9628116583838917 1104 1 0.2298762980239637 1104 2 0.2545160299603495 1104 3 0.1862631099439227 1104 4 0.13863144416038864 1104 5 0.114901936332096 1104 6 0.13576291957149067 1104 7 0.19843905666803607 1104 8 0.20017195725470316 1104 9 0.23595437604227953 1104 10-11 0.24594130046632046 1104 12-13 0.24836443490608673 1104 14-15 0.39842805773918855 1104 16-17 0.4016450408836221 1104 18-19 0.34791553052014734 1104 20-21 0.41450723963489366 1104 22-23 0.36460429465972055 1104 24-25 0.4045577653530472 1104 26-27 0.39855923147723615 1104 28-29 0.38216920715962743 1104 30-31 0.3826503592009516 1104 32-33 0.3789370303149653 1104 34-35 0.35827637979451765 1104 36-37 0.48375611002294505 1104 38-39 0.49298068185313326 1104 40-41 0.6256900443713178 1104 42-43 0.7220595551816729 1104 44-45 0.5246737325680755 1104 46-47 0.46698487504574615 1104 48-49 0.5261008722836706 1104 50-51 0.657757725623398 1104 52-53 0.5733883873994827 1104 54-55 0.5468589982440548 1104 56-57 0.49264693008100835 1104 58-59 0.5213334603119719 1104 60-61 0.6369651746928753 1104 62-63 0.5862020582275207 1104 64-65 0.5496013351083704 1104 66-67 0.6306557663071395 1104 68-69 0.6195544980834455 1104 70-71 0.6934921490652854 1104 72-73 0.6185643726198933 1104 74-75 0.7038113005101394 1104 76-77 0.6926971870395136 1104 78-79 0.9241861801381006 1104 80-81 0.9101254028240717 1104 82-83 0.9670778532422091 1104 84-85 0.981797844829881 1104 86-87 1.0124849147171275 1104 88-89 0.9695548998942485 1104 90-91 0.9687659056322602 1104 92-93 1.2191630086127496 1104 94-95 1.2382764069951762 1104 96-97 1.2084938008228256 1104 98-99 1.2917654346558365 1104 100-101 1.5615544396650094 1105 1 0.11304409453160602 1105 2 0.1954790250945848 1105 3 0.1773838752806256 1105 4 0.1244595971667053 1105 5 -0.007876854769534702 1105 6 0.023225722727175935 1105 7 0.08882351838609992 1105 8 0.13891367681281963 1105 9 0.13966105031340703 1105 10-11 0.1708490958196407 1105 12-13 0.13019579612522847 1105 14-15 0.2033492057101185 1105 16-17 0.22082487693746344 1105 18-19 0.19340154883806804 1105 20-21 0.20600023745863894 1105 22-23 0.22441717238245218 1105 24-25 0.21576433053939326 1105 26-27 0.08594704617883053 1105 28-29 0.04769061695535726 1105 30-31 0.14160109907316354 1105 32-33 0.24554388971549912 1105 34-35 0.3477092381369573 1105 36-37 0.309674985296418 1105 38-39 0.34720148417731167 1105 40-41 0.30742187972786184 1105 42-43 0.3961218501142838 1105 44-45 0.2461209638575781 1105 46-47 0.2096414954572623 1105 48-49 0.366657165206135 1105 50-51 0.2727780548663361 1105 52-53 0.2523310128573897 1105 54-55 0.2525116597047301 1105 56-57 0.2108951767498155 1105 58-59 0.17624846859654753 1105 60-61 0.23719094018801457 1105 62-63 0.23928502891396164 1105 64-65 0.23044982375869694 1105 66-67 0.19918778810532345 1105 68-69 0.2701386004244952 1105 70-71 0.3182601772060636 1105 72-73 0.29340007600017515 1105 74-75 0.37323591855070504 1105 76-77 0.35666916617247324 1105 78-79 0.5643432174188519 1105 80-81 0.5724949824971972 1105 82-83 0.5824903009415223 1105 84-85 0.6044479212212366 1105 86-87 0.559421509946862 1105 88-89 0.5481284827282202 1105 90-91 0.7807352108277996 1105 92-93 0.9327937950804355 1105 94-95 0.9891313489839426 1105 96-97 1.294361021650861 1105 98-99 1.529461764595645 1105 100-101 1.7972486770972935 1106 1 0.05230890227876728 1106 2 0.12899433630082768 1106 3 0.18051698973839336 1106 4 0.11800496980874442 1106 5 0.02308448969773025 1106 6 0.06690020643723926 1106 7 0.14557137233131812 1106 8 0.07616567717006717 1106 9 -0.20566321108444185 1106 10-11 0.08251785328715044 1106 12-13 0.09250031344862464 1106 14-15 0.10283200002428572 1106 16-17 0.18918031802029844 1106 18-19 0.09479641756828983 1106 20-21 0.19897918004846105 1106 22-23 0.22447273500337417 1106 24-25 0.21969260783885147 1106 26-27 0.2389161377217448 1106 28-29 0.33252236099195187 1106 30-31 0.27442349294958746 1106 32-33 0.20165106548394363 1106 34-35 0.2388175621995714 1106 36-37 0.37183664494779123 1106 38-39 0.2396110539538796 1106 40-41 0.22952349676476302 1106 42-43 0.24526490986961846 1106 44-45 0.1480189315415359 1106 46-47 -0.45178937167769107 1106 48-49 -0.17948903198519162 1106 50-51 0.1780072589091617 1106 52-53 0.20399065805506922 1106 54-55 0.14368568142189275 1106 56-57 0.2149489329681984 1106 58-59 0.08917690270607181 1106 60-61 0.32807796038935066 1106 62-63 0.17575647734094701 1106 64-65 0.27896381175141016 1106 66-67 0.21711460472521438 1106 68-69 0.028548569017203818 1106 70-71 0.11882668957327525 1106 72-73 0.40179276873353587 1106 74-75 0.36211135579766207 1106 76-77 0.3389904263656405 1106 78-79 0.7015064925019843 1106 80-81 0.05352353715703018 1106 82-83 0.32512007361631845 1106 84-85 0.5544746427261487 1106 86-87 0.8591744414924811 1106 88-89 0.8782150040183758 1106 90-91 0.8857713553416495 1106 92-93 0.9070964430303192 1106 94-95 1.0216421188719629 1106 96-97 1.3362644982657876 1106 98-99 1.1507163294765306 1106 100-101 1.1275565228274687 1107 1 0.08749560636741549 1107 2 0.05568155865055502 1107 3 0.05112783877392957 1107 4 0.023142511911387942 1107 5 -0.018713684019346033 1107 6 0.049978952994948145 1107 7 0.015403276320391512 1107 8 0.03505227216952278 1107 9 0.11188529555081317 1107 10-11 0.14174092963367002 1107 12-13 0.10734313412485363 1107 14-15 0.20063275941560477 1107 16-17 0.13437066488822325 1107 18-19 0.11455616501261545 1107 20-21 0.20876070261368795 1107 22-23 0.15691490782413098 1107 24-25 0.20342398665410855 1107 26-27 0.12295813337448891 1107 28-29 0.10964606635867824 1107 30-31 0.14134005699317953 1107 32-33 0.2004320160007822 1107 34-35 0.1773904684655747 1107 36-37 0.12452770930342894 1107 38-39 0.1213335448537265 1107 40-41 0.05166615778784234 1107 42-43 0.0753281885962167 1107 44-45 -0.0761664140778322 1107 46-47 -0.11330163188242892 1107 48-49 0.052177943424489115 1107 50-51 0.038441530238742416 1107 52-53 0.043030251725951985 1107 54-55 0.05741919231159187 1107 56-57 -0.023980542358962964 1107 58-59 -0.06697153006387424 1107 60-61 -0.09174036084863957 1107 62-63 0.10741329724123005 1107 64-65 -0.1022714865467762 1107 66-67 -0.12072710486450333 1107 68-69 -0.003099775013392758 1107 70-71 0.3348808799927667 1107 72-73 0.3245727413699697 1107 74-75 0.21762632167864737 1107 76-77 0.1863283139497831 1107 78-79 0.5080599126173482 1107 80-81 0.43192140943217794 1107 82-83 0.45450509829828434 1107 84-85 0.6084568548562963 1107 86-87 0.7426682241071028 1107 88-89 0.6302286298769815 1107 90-91 0.6656590874474659 1107 92-93 0.7408522591468589 1107 94-95 0.8062923576914862 1107 96-97 0.805900690248798 1107 98-99 0.9683960518951977 1107 100-101 1.1820273353497797 1108 1 0.1762119846384671 1108 2 0.1586036191945226 1108 3 0.14779007054561788 1108 4 0.1605363229389809 1108 5 0.09965468243551356 1108 6 0.21124027280950486 1108 7 0.17638805239642608 1108 8 0.155594119481691 1108 9 0.214846862343137 1108 10-11 0.25335340902032755 1108 12-13 0.3422511100540717 1108 14-15 0.3513780893947356 1108 16-17 0.4619475555170851 1108 18-19 0.3084311672428015 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2316 64-65 -0.9945286734248953 2316 66-67 -1.0393210506944932 2316 68-69 -0.9417126377609826 2316 70-71 -0.8736286317904103 2316 72-73 -0.8400782259947519 2316 74-75 -0.9625413210888674 2316 76-77 -0.9593449611578677 2316 78-79 -1.0615676563129206 2316 80-81 -1.16990572579315 2316 82-83 -1.2499306957470822 2316 84-85 -1.3872891456262728 2316 86-87 -1.4803844717238412 2316 88-89 -1.7012754503833953 2316 90-91 -1.4428214552056033 2316 92-93 -1.7364541714705624 2316 94-95 -1.5692761520320815 2316 96-97 -1.6670545089536795 2316 98-99 -1.5538581019225646 2316 100-101 -1.9243289683102418 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 13.0 4 46.0 5 84.0 6 204.0 7 599.0 8 1347.0 9 2587.0 10 4526.0 11 6752.0 12 9581.0 13 13344.0 14 17141.0 15 22167.0 16 27527.0 17 34379.0 18 32080.0 19 26937.0 20 25925.0 21 27112.0 22 29303.0 23 33453.0 24 38960.0 25 45590.0 26 54167.0 27 65412.0 28 78414.0 29 93934.0 30 111986.0 31 134295.0 32 162602.0 33 202191.0 34 258921.0 35 343065.0 36 459507.0 37 539403.0 38 291228.0 39 14373.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.61902936763531 19.84460035305354 15.776819059282147 22.75955122002901 2 15.35307884956351 21.066040885882614 39.61396259344857 23.96691767110531 3 18.501595899987567 24.943840461728158 31.0075748770473 25.54698876123697 4 12.8883842225429 17.687862964806065 37.65544950845408 31.768303304196955 5 12.994701836042166 38.247381703175 34.74264390695557 14.015272553827257 6 31.150073181121172 37.69134674140687 17.17585998334144 13.98272009413052 7 27.627220946534 33.77125672410951 21.03281823504648 17.568704094310007 8 26.472339655131044 34.76385260820507 20.131897402467498 18.631910334196384 9 26.304663430722236 16.693116986028436 20.42184857662174 36.58037100662759 10-11 24.37939386649657 27.0040985790367 27.852170707546303 20.764336846920425 12-13 25.809954689091636 24.389256257320838 27.918745740709177 21.88204331287835 14-15 22.124988431555785 25.970993664699275 26.468257591314366 25.43576031243057 16-17 21.350035102632745 29.027477898428906 27.68502421660989 21.937462782328467 18-19 21.51135452537283 27.25706189250087 30.07544651078584 21.156137071340453 20-21 21.983985845521616 26.39426628953798 30.311879023832084 21.309868841108315 22-23 21.814626938318145 26.380758085991946 30.375135432594373 21.429479543095532 24-25 21.69824006377161 26.73080288062939 30.234782295712474 21.33617475988652 26-27 21.02298231823745 27.098709216532065 29.969095672537215 21.90921279269327 28-29 21.327317630037676 27.54878112052186 29.815778694494956 21.308122554945513 30-31 21.445939389444614 26.904242327548033 29.538538866371404 22.111279416635945 32-33 20.85547316608645 27.178335184291907 29.531825892729163 22.434365756892483 34-35 22.12671144185733 27.184800660439777 29.19617822524574 21.49230967245715 36-37 22.27963383732129 26.434760520008354 29.38305889833282 21.902546744337535 38-39 21.486239252556622 26.916605978337927 29.34558816884537 22.251566600260077 40-41 22.006012234495216 26.88567873528064 29.446845758171314 21.66146327205283 42-43 21.810351556809415 26.876224151139656 29.026351998053045 22.287072293997888 44-45 21.665650479503743 27.112096844688217 29.4314966496987 21.790756026109335 46-47 22.380522510400695 27.476948024732945 28.36800646648766 21.774522998378703 48-49 21.923471586498778 27.630492829745844 28.11625842393074 22.32977715982464 50-51 22.169393954549513 27.612627119317462 28.379639345799912 21.838339580333113 52-53 22.59804042240324 27.152455112099393 28.41103952092151 21.838464944575854 54-55 21.67721578154233 27.603131204979814 28.573024471536712 22.146628541941148 56-57 22.04291090412728 27.654361996796045 28.16360724812498 22.139119850951698 58-59 21.90259187636564 27.11252564923918 28.252710010523845 22.73217246387133 60-61 21.24463372202267 27.64202232296853 28.713221306129544 22.400122648879258 62-63 21.558283837515976 27.64540457863968 28.734655361706672 22.061656222137664 64-65 21.158236901893932 27.64904150317733 28.452925978676525 22.739795616252216 66-67 21.108723864702807 27.67716423173246 28.367202921952206 22.846908981612522 68-69 21.885199995886108 27.158367046983443 28.498093117927496 22.45833983920295 70-71 21.740410060896046 27.213840392688738 28.01496966104032 23.0307798853749 72-73 21.510349679004538 27.395367787339826 28.16561667665112 22.928665857004525 74-75 21.599497448359813 27.929983747484943 27.841264245885018 22.62925455827023 76-77 21.583863227952996 27.272524626226325 28.37213130495617 22.77148084086451 78-79 21.626907530689202 27.556576212781575 28.142860148573686 22.67365610795554 80-81 22.00576034550229 27.604759446120386 27.776999418230353 22.61248079014698 82-83 21.896890858988264 27.600048005586103 27.965654185214277 22.53740695021135 84-85 21.5570860061939 27.83471257769255 27.993860952347116 22.614340463766435 86-87 21.89794340459361 27.85000344360451 27.46033076678321 22.791722385018666 88-89 21.54868566914444 27.825550536318843 27.287602159590314 23.338161634946403 90-91 21.46751242233508 27.653386461633982 27.662066979310758 23.21703413672018 92-93 21.689554396403043 27.333932078151 27.784315348080035 23.19219817736592 94-95 21.19405576314316 27.006804883856915 28.08504484017001 23.714094512829913 96-97 21.47461250293219 26.53055594305046 28.221289290359586 23.773542263657767 98-99 21.132060294739365 27.051368628727424 27.60949216884223 24.207078907690985 100-101 21.139810320932295 26.69405173399803 28.159543962429446 24.00659398264023 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 427.0 1 315.0 2 273.0 3 363.5 4 361.5 5 385.0 6 650.5 7 1526.5 8 2496.0 9 2807.0 10 2458.5 11 2078.5 12 2093.0 13 2301.0 14 2571.5 15 2736.5 16 3062.0 17 3550.0 18 3902.5 19 4382.5 20 5042.5 21 5928.5 22 7223.5 23 8941.0 24 11089.5 25 14046.0 26 18260.5 27 23401.5 28 28815.5 29 35245.5 30 42669.5 31 52581.5 32 68045.5 33 74146.0 34 71818.5 35 76007.0 36 84267.5 37 91496.5 38 96010.5 39 99278.5 40 103060.0 41 107626.5 42 109762.5 43 111035.5 44 112875.0 45 118433.5 46 119940.0 47 121290.0 48 124569.0 49 118897.0 50 111053.0 51 104751.5 52 99906.5 53 100060.5 54 102151.0 55 96017.5 56 86000.0 57 76628.0 58 70090.0 59 60775.0 60 45873.5 61 37657.5 62 33916.0 63 24847.5 64 15381.5 65 11020.0 66 7771.0 67 5706.5 68 4559.5 69 3672.5 70 2967.5 71 2051.0 72 1551.0 73 1333.0 74 1004.5 75 764.5 76 520.5 77 322.0 78 204.5 79 125.5 80 77.5 81 34.0 82 14.5 83 11.0 84 4.0 85 1.0 86 1.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.985726798998243E-4 2 0.0513529860296819 3 0.0 4 0.0 5 0.04505850594594662 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006528186027438324 26-27 0.0019787103233524276 28-29 1.4022356622182556E-4 30-31 0.0015580396246869508 32-33 4.0509030241860723E-4 34-35 0.012152709072558218 36-37 0.023401755162798 38-39 0.0044248325341109404 40-41 0.0011841101147620826 42-43 0.0015424592284400815 44-45 0.008428994369556403 46-47 0.0 48-49 0.0 50-51 6.387962461216499E-4 52-53 0.0 54-55 0.0014022356622182559 56-57 0.0 58-59 0.022825280501663832 60-61 0.0 62-63 0.00947288091809666 64-65 0.015299949114425858 66-67 0.006559346819932063 68-69 0.01620361209674429 70-71 1.0906277372808657E-4 72-73 1.0906277372808657E-4 74-75 0.022887602086651308 76-77 0.050013071952451124 78-79 0.03080244338006102 80-81 0.026377610845950077 82-83 0.037424111784980556 84-85 0.01620361209674429 86-87 0.009784488843034051 88-89 0.08474177518672325 90-91 0.025769975392322165 92-93 0.02438332012635078 94-95 0.02732801501700912 96-97 0.03843683754102708 98-99 0.0535030807117499 100-101 0.014894858812007252 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3209161.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.84822921034578 #Duplication Level Percentage of deduplicated Percentage of total 1 66.38949129466614 27.11893157562778 2 15.890577109592302 12.982038721146003 3 6.742913390513033 8.263082151635585 4 3.443180271824587 5.6259126782412565 5 2.0463527454613257 4.17949429959123 6 1.3155909858665633 3.224373728264529 7 0.8701123824111817 2.487978502484439 8 0.6084825658785704 1.9884348257205735 9 0.42421473717996666 1.5595578736860483 >10 2.0386728111387176 14.488074666320088 >50 0.13946589405706317 3.8943923628161228 >100 0.07802106715484025 5.968064031877822 >500 0.007265388783213311 2.012750202116424 >1k 0.005124011035941842 4.701280136080235 >5k 4.588666599350903E-4 1.1837704046547168 >10k+ 7.647777665584839E-5 0.3218638397370899 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10303 0.3210496450629931 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8627 0.26882415684348654 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6186 0.19276066236626957 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6157 0.19185699938395112 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5951 0.1854378761302409 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5628 0.17537294015476318 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5344 0.1665232750865413 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4928 0.15356038540914588 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4872 0.1518153810294965 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4872 0.1518153810294965 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 4233 0.13190363462599727 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3922 0.12221262816044443 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3919 0.1221191457829632 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3886 0.12109083963066983 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3809 0.11869145860865192 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3591 0.1118984058450168 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3530 0.10999759750289873 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3453 0.10759821648088083 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3407 0.10616482002616884 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3335 0.10392124296661961 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.1160792493739015E-5 2 0.0 0.0 0.0 3.1160792493739015E-5 3.1160792493739015E-5 3 0.0 0.0 0.0 3.1160792493739015E-5 3.1160792493739015E-5 4 0.0 0.0 0.0 6.232158498747803E-5 3.1160792493739015E-5 5 0.0 0.0 0.0 6.232158498747803E-5 3.1160792493739015E-5 6 0.0 0.0 0.0 6.232158498747803E-5 3.1160792493739015E-5 7 0.0 0.0 0.0 6.232158498747803E-5 3.1160792493739015E-5 8 0.0 0.0 0.0 6.232158498747803E-5 3.1160792493739015E-5 9 0.0 0.0 0.0 6.232158498747803E-5 3.1160792493739015E-5 10-11 0.0 0.0 0.0 6.232158498747803E-5 3.1160792493739015E-5 12-13 0.0 0.0 0.0 6.232158498747803E-5 9.348237748121706E-5 14-15 0.0 0.0 0.0 6.232158498747803E-5 9.348237748121706E-5 16-17 0.0 0.0 0.0 1.0906277372808656E-4 9.348237748121706E-5 18-19 0.0 0.0 0.0 1.713843587155646E-4 9.348237748121706E-5 20-21 0.0 0.0 0.0 1.8696475496243412E-4 1.2464316997495606E-4 22-23 0.0 0.0 0.0 4.050903024186072E-4 1.2464316997495606E-4 24-25 0.0 0.0 0.0 9.659845673059095E-4 1.2464316997495606E-4 26-27 0.0 0.0 0.0 0.001838486757130602 1.2464316997495606E-4 28-29 0.0 0.0 0.0 0.005437558290157459 1.2464316997495606E-4 30-31 0.0 0.0 0.0 0.016250353285484898 1.2464316997495606E-4 32-33 0.0 0.0 0.0 0.04049344984561386 1.2464316997495606E-4 34-35 0.0 0.0 0.0 0.07706063983701658 1.558039624686951E-4 36-37 0.0 0.0 0.0 0.13656217310381125 1.558039624686951E-4 38-39 0.0 0.0 0.0 0.22753610678928232 1.558039624686951E-4 40-41 0.0 0.0 0.0 0.32534983442712906 1.558039624686951E-4 42-43 0.0 0.0 0.0 0.4252201743695626 1.558039624686951E-4 44-45 0.0 0.0 0.0 0.5541946945011484 1.558039624686951E-4 46-47 0.0 0.0 0.0 0.7057296283982013 1.558039624686951E-4 48-49 0.0 0.0 0.0 0.8590563078636442 1.8696475496243412E-4 50-51 0.0 0.0 0.0 1.0323726357138205 2.1812554745617312E-4 52-53 0.0 0.0 0.0 1.1968860396845158 2.1812554745617312E-4 54-55 0.0 0.0 0.0 1.379410381716592 2.1812554745617312E-4 56-57 0.0 0.0 0.0 1.5953390933019564 2.1812554745617312E-4 58-59 0.0 0.0 0.0 1.819400771728187 2.1812554745617312E-4 60-61 0.0 0.0 0.0 2.06286004348177 2.3370594370304262E-4 62-63 0.0 0.0 0.0 2.305010561950616 2.492863399499121E-4 64-65 0.0 0.0 0.0 2.557833651848567 2.492863399499121E-4 66-67 0.0 0.0 0.0 2.8623524964936315 2.6486673619678165E-4 68-69 0.0 0.0 0.0 3.197580302141276 2.804471324436512E-4 70-71 0.0 0.0 0.0 3.5525328894374573 2.804471324436512E-4 72-73 0.0 0.0 0.0 3.9208690371097 2.804471324436512E-4 74-75 0.0 0.0 0.0 4.300127042550997 2.804471324436512E-4 76-77 0.0 0.0 0.0 4.706728643405551 2.804471324436512E-4 78-79 0.0 0.0 0.0 5.1668489053681 2.9602752869052065E-4 80-81 0.0 0.0 0.0 5.6428144303137175 3.116079249373902E-4 82-83 0.0 0.0 0.0 6.140841796344901 3.7392950992486823E-4 84-85 0.0 0.0 0.0 6.657643539853563 3.7392950992486823E-4 86-87 0.0 0.0 0.0 7.180677441860972 3.895099061717377E-4 88-89 0.0 0.0 0.0 7.736741783911745 4.0509030241860723E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9325 0.0 36.802464 1 GTATCAA 14300 0.0 34.037094 1 TAAGGTG 2780 0.0 30.759499 5 GGTAAGG 2860 0.0 27.07529 3 GTAAGGT 2950 0.0 26.410303 4 ATCAACG 18240 0.0 26.409773 3 TATCAAC 18600 0.0 26.091637 2 AGGTAAG 2755 0.0 26.05217 2 TCAACGC 18515 0.0 26.017513 4 CAACGCA 18545 0.0 26.00104 5 TAAGGTA 1335 0.0 25.265543 4 AACGCAG 19165 0.0 25.147346 6 TACATGG 11660 0.0 24.092257 2 AAGGTAA 3015 0.0 23.963202 1 GTACATG 12020 0.0 23.884768 1 GTGTAAG 1445 0.0 23.026012 1 ACATGGG 12025 0.0 22.795141 3 ACGCAGA 21310 0.0 22.50468 7 CGCAGAG 21430 0.0 22.356506 8 GAGTACT 12445 0.0 21.61424 12-13 >>END_MODULE