FastQCFastQC Report
Thu 26 May 2016
SRR938518_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938518_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3229968
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112950.349693866936143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87810.2718602784919231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60170.1862866752859471No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA58120.17993986318130706No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA57730.1787324208784731No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC56670.1754506546194885No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA55810.17278808954144437No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA49700.15387149346371234No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48630.1505587671456807No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG43920.1359765793345321No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA41970.1299393678203623No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT41830.12950592699370397No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA40120.12421175689666275No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG39450.12213743294051212No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA38470.1191033471539037No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT34070.10548092117321287No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33780.10458307946084915No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG33420.10346851733515625No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT32920.10192051438280504No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94600.037.3987271
GTATCAA149700.036.1339721
ATCAACG184950.029.0867923
TCAACGC186150.028.8992844
CAACGCA186550.028.8364265
TATCAAC187700.028.7619132
AACGCAG192400.028.0527886
TAAGGTG24050.026.4776825
ACGCAGA214200.024.8175897
GTACATG122300.024.6064971
CGCAGAG216700.024.5316568
GGTAAGG22900.024.4878273
TACATGG122250.024.2571322
GTAAGGT25150.023.4308174
ACATGGG123400.023.3378683
AGGTAAG23400.023.1522242
GCAGAGT225850.022.6122659
GAGTACT121650.022.29732512-13
GTACTTT124400.021.95716514-15
GCGCGAC1302.9402607E-421.9262129
Produced by FastQC (version 0.11.5)