##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938518_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3229968 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.172533288255487 31.0 31.0 34.0 28.0 34.0 2 31.271441698493607 31.0 31.0 34.0 28.0 34.0 3 31.332347565053276 31.0 31.0 34.0 28.0 34.0 4 34.975366629019234 37.0 35.0 37.0 32.0 37.0 5 35.013671962075165 37.0 35.0 37.0 32.0 37.0 6 35.054242023450385 37.0 35.0 37.0 32.0 37.0 7 34.984690870002424 37.0 35.0 37.0 32.0 37.0 8 34.93808328751245 37.0 35.0 37.0 32.0 37.0 9 36.46249343646748 39.0 37.0 39.0 32.0 39.0 10-11 36.31797869204896 39.0 35.0 39.0 32.0 39.0 12-13 36.19385393291822 39.0 35.0 39.0 32.0 39.0 14-15 37.20420372585735 39.0 36.0 41.0 31.0 41.0 16-17 37.09347290747153 39.0 36.0 41.0 31.0 41.0 18-19 37.11437497213595 39.0 36.0 41.0 31.0 41.0 20-21 37.19289819589544 39.0 36.0 41.0 31.0 41.0 22-23 37.1271735509454 39.0 36.0 41.0 31.0 41.0 24-25 37.02167142213174 39.0 36.0 41.0 30.5 41.0 26-27 36.849650987254364 39.0 36.0 41.0 30.0 41.0 28-29 36.68780449218073 39.0 36.0 41.0 30.0 41.0 30-31 36.45341470875253 39.0 35.5 40.5 30.0 41.0 32-33 36.15284160709952 39.0 35.0 40.0 29.0 41.0 34-35 35.91279526608313 38.0 35.0 40.0 28.5 41.0 36-37 35.620776583545094 38.0 35.0 40.0 27.0 41.0 38-39 35.35013319017402 38.0 34.0 40.0 26.5 41.0 40-41 35.27056630282405 38.0 34.0 40.0 26.0 41.0 42-43 35.08951172271676 38.0 34.0 40.0 25.0 41.0 44-45 34.80780088223784 38.0 34.0 40.0 24.5 41.0 46-47 34.44582593387922 38.0 33.0 40.0 23.5 41.0 48-49 34.082814907144595 38.0 33.0 40.0 22.5 41.0 50-51 33.33127557300877 37.5 32.0 39.5 21.5 40.5 52-53 33.38772845427571 37.5 32.5 39.5 21.0 40.5 54-55 33.53914574385877 38.0 33.0 40.0 20.5 41.0 56-57 33.27379063817351 37.0 32.5 40.0 18.5 41.0 58-59 32.97225034427586 37.0 32.0 40.0 17.5 41.0 60-61 32.552874053241396 36.5 31.0 39.5 14.0 41.0 62-63 32.076913765089934 36.0 31.0 39.0 10.0 41.0 64-65 31.617871446404422 35.5 30.5 39.0 8.0 40.0 66-67 31.11602746528758 35.0 30.0 38.5 7.0 40.0 68-69 30.602770368003647 35.0 29.0 38.0 2.0 40.0 70-71 29.997490377613648 34.0 28.5 37.5 2.0 39.5 72-73 29.485179574534484 34.0 28.0 37.0 2.0 39.0 74-75 28.933550115666783 34.0 27.5 36.0 2.0 39.0 76-77 28.24057498402461 33.5 26.0 36.0 2.0 38.5 78-79 27.49892692435343 33.0 25.5 35.0 2.0 37.0 80-81 26.894628058234634 32.5 24.5 35.0 2.0 37.0 82-83 26.258029800914436 32.0 23.5 35.0 2.0 36.0 84-85 25.638529391003253 32.0 20.5 35.0 2.0 36.0 86-87 25.025784465976134 31.0 19.0 34.0 2.0 35.0 88-89 24.268115349749596 31.0 14.0 34.0 2.0 35.0 90-91 23.47176891535768 30.5 4.5 34.0 2.0 35.0 92-93 22.672964097477127 30.0 2.0 34.0 2.0 35.0 94-95 21.752788572518366 29.0 2.0 34.0 2.0 35.0 96-97 20.567124349219558 29.0 2.0 34.0 2.0 35.0 98-99 19.117597449881856 26.5 2.0 33.5 2.0 35.0 100-101 15.580028656630654 12.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6796909158779307 1101 2 0.6728795027187289 1101 3 0.7150977478716705 1101 4 0.6257971503728328 1101 5 0.6453981139447791 1101 6 0.6224427564305373 1101 7 0.6019522548196647 1101 8 0.6434534145424422 1101 9 0.7119397701789012 1101 10-11 0.7281984246612296 1101 12-13 0.8225225136402088 1101 14-15 1.0110403184396901 1101 16-17 0.8963189939527965 1101 18-19 0.8763697516500244 1101 20-21 0.9110942218621432 1101 22-23 0.9685715076778934 1101 24-25 1.068361370006535 1101 26-27 0.989734639909166 1101 28-29 1.0709328457235685 1101 30-31 1.0645183919889192 1101 32-33 1.1610633238122006 1101 34-35 1.1521743490148282 1101 36-37 1.2037740043875473 1101 38-39 1.1624025832987925 1101 40-41 1.2486724144384382 1101 42-43 1.2065021183718514 1101 44-45 1.3579691751336895 1101 46-47 1.2640103124232454 1101 48-49 1.4029621896080826 1101 50-51 1.2120711260783992 1101 52-53 1.3369777722381926 1101 54-55 1.4757616215987994 1101 56-57 1.3905688150697628 1101 58-59 1.4145527365259127 1101 60-61 1.3798315298322734 1101 62-63 1.2374871910583636 1101 64-65 1.1139601771541727 1101 66-67 1.136106634514146 1101 68-69 1.1531456150164665 1101 70-71 1.2549760143828124 1101 72-73 1.2231240552933258 1101 74-75 1.2758689678231825 1101 76-77 1.5097790453003732 1101 78-79 1.420146799883554 1101 80-81 1.405629340546426 1101 82-83 1.4360922728395558 1101 84-85 1.7453004832146313 1101 86-87 1.834086698896222 1101 88-89 1.8957812106641256 1101 90-91 1.7203117631361025 1101 92-93 1.5449713742003937 1101 94-95 1.3140960899604686 1101 96-97 1.3267497828384158 1101 98-99 1.359822693934838 1101 100-101 1.11435970269204 1102 1 0.5903050532536902 1102 2 0.6827533051337582 1102 3 0.6731438316673781 1102 4 0.6252546865620019 1102 5 0.5527200939803976 1102 6 0.6179385982532111 1102 7 0.502254240578921 1102 8 0.5680851157858342 1102 9 0.5923686796557917 1102 10-11 0.7577410785479657 1102 12-13 0.806609263000567 1102 14-15 0.8261293017258211 1102 16-17 0.8441733253341184 1102 18-19 0.8147862271729451 1102 20-21 0.7215001834112869 1102 22-23 0.8089459251700006 1102 24-25 0.5893759695443777 1102 26-27 0.5944381642153829 1102 28-29 0.5719163283412314 1102 30-31 0.5480736229204624 1102 32-33 0.568507037852612 1102 34-35 0.6597832582354286 1102 36-37 0.7312143440467835 1102 38-39 0.695289451830341 1102 40-41 0.6902048533471898 1102 42-43 0.7772381178934609 1102 44-45 0.8614145678219529 1102 46-47 0.7354185719686868 1102 48-49 0.6532214082893404 1102 50-51 0.6831179210569758 1102 52-53 0.751221772033162 1102 54-55 0.7028972525013018 1102 56-57 0.7311995146772219 1102 58-59 0.7268231504642486 1102 60-61 0.7011599147850944 1102 62-63 0.7068790976691588 1102 64-65 0.5934602775313316 1102 66-67 0.5845258543913836 1102 68-69 0.512823681965898 1102 70-71 0.4729436865296428 1102 72-73 0.6820041238060774 1102 74-75 0.5112985286837812 1102 76-77 0.6555851486589503 1102 78-79 0.7079801802019432 1102 80-81 0.7331343914397799 1102 82-83 0.6227233162921912 1102 84-85 0.4302737017335794 1102 86-87 0.49159822091299077 1102 88-89 0.6800917797385644 1102 90-91 0.8055546651893515 1102 92-93 0.8403506610526925 1102 94-95 0.7797847200044181 1102 96-97 1.0821669460516787 1102 98-99 1.3111341642680472 1102 100-101 0.9732651858478949 1103 1 0.44505770800634536 1103 2 0.637253434633891 1103 3 0.5293663128898594 1103 4 0.48719459850191527 1103 5 0.48749087875118846 1103 6 0.5246596549742719 1103 7 0.4089882473129265 1103 8 0.42953292723364456 1103 9 0.4971019093890163 1103 10-11 0.5544809127877954 1103 12-13 0.479372148263451 1103 14-15 0.611544349640873 1103 16-17 0.6140872077479997 1103 18-19 0.6714749088616259 1103 20-21 0.5823555317666376 1103 22-23 0.6340462877703601 1103 24-25 0.6886054437738522 1103 26-27 0.6070709018481253 1103 28-29 0.5106886671135697 1103 30-31 0.7144035892504306 1103 32-33 0.7710322903778675 1103 34-35 0.7328342812864506 1103 36-37 0.6450579078903402 1103 38-39 0.755960262501155 1103 40-41 0.6286275417698803 1103 42-43 0.4114779521332892 1103 44-45 0.5161348475422329 1103 46-47 0.5721350961852139 1103 48-49 0.5360335036014376 1103 50-51 0.7091247845638406 1103 52-53 0.7529214612328516 1103 54-55 0.6450398321438868 1103 56-57 0.6332618042395097 1103 58-59 0.49164120278229717 1103 60-61 0.4856749368001161 1103 62-63 0.5752745910646553 1103 64-65 0.44684670591776054 1103 66-67 0.5451999025654324 1103 68-69 0.5330192271614429 1103 70-71 0.5826788587648153 1103 72-73 0.6440800108819644 1103 74-75 0.5939422738275262 1103 76-77 0.48176036233416397 1103 78-79 0.5164138761356369 1103 80-81 0.5754938837992754 1103 82-83 0.6387683823372576 1103 84-85 0.7867033831632604 1103 86-87 0.8047813340961021 1103 88-89 0.5825296196764036 1103 90-91 0.8024434245781116 1103 92-93 0.7780772862793199 1103 94-95 0.631727221946921 1103 96-97 0.501611390496123 1103 98-99 0.6142389298728119 1103 100-101 0.5069093194920331 1104 1 0.5064879582885062 1104 2 0.575697519666992 1104 3 0.5392507711936183 1104 4 0.5671987873247346 1104 5 0.43366566901915604 1104 6 0.44977513849242 1104 7 0.4246148835718415 1104 8 0.47888075079112014 1104 9 0.4107022026066147 1104 10-11 0.5249409851613507 1104 12-13 0.46964244369182495 1104 14-15 0.6386215985151225 1104 16-17 0.5674094678506236 1104 18-19 0.6026016440233022 1104 20-21 0.637592723859072 1104 22-23 0.6675088734376118 1104 24-25 0.5690852385215308 1104 26-27 0.5762987621741544 1104 28-29 0.6377944232542703 1104 30-31 0.5900077687647567 1104 32-33 0.5972621753015943 1104 34-35 0.6035750629673373 1104 36-37 0.728483259576926 1104 38-39 0.5572640336052572 1104 40-41 0.7593531389680166 1104 42-43 0.7577635716718234 1104 44-45 0.777234355355553 1104 46-47 0.8710503656079638 1104 48-49 0.8610672468964111 1104 50-51 0.8985203723371313 1104 52-53 0.6855276653448854 1104 54-55 0.8920785671193912 1104 56-57 0.8213341312469495 1104 58-59 0.8832661751477033 1104 60-61 0.9653539100730981 1104 62-63 0.8854909553991561 1104 64-65 0.8580736237586528 1104 66-67 0.8223184578869827 1104 68-69 0.9302918380779808 1104 70-71 0.8946478382046799 1104 72-73 0.8747424848472711 1104 74-75 0.7805077881800635 1104 76-77 0.9343041995285866 1104 78-79 0.8855605780194473 1104 80-81 1.0695364468934194 1104 82-83 0.9623406918912636 1104 84-85 0.8684319707154735 1104 86-87 1.0196964522558183 1104 88-89 1.0349396472528234 1104 90-91 1.0079229284353168 1104 92-93 0.9354865575405036 1104 94-95 0.9158236579568353 1104 96-97 0.8323422237227973 1104 98-99 0.6948874410953678 1104 100-101 0.5067208224649331 1105 1 0.4795675145622589 1105 2 0.4833528683413917 1105 3 0.48539187554366237 1105 4 0.4459378725189609 1105 5 0.5094616393386602 1105 6 0.5097770671810196 1105 7 0.37833903741299935 1105 8 0.3659762789507752 1105 9 0.40695873524008164 1105 10-11 0.5050095868034958 1105 12-13 0.5073690313035684 1105 14-15 0.6759465180776161 1105 16-17 0.755706963272651 1105 18-19 0.6240446653939173 1105 20-21 0.5640768683871045 1105 22-23 0.6902355915821445 1105 24-25 0.6994546250120806 1105 26-27 0.7567133023550809 1105 28-29 0.7604862438563913 1105 30-31 0.670242067135014 1105 32-33 0.6653721084208541 1105 34-35 0.7333267363178066 1105 36-37 0.7383707189985458 1105 38-39 0.704990019167802 1105 40-41 0.7780272135038402 1105 42-43 0.9788881995867698 1105 44-45 0.847263972979718 1105 46-47 0.7496240737462401 1105 48-49 0.7674324520753117 1105 50-51 0.6346289393907654 1105 52-53 0.7983180675084256 1105 54-55 0.7114611949629435 1105 56-57 0.8753497195262767 1105 58-59 0.9613522234299197 1105 60-61 0.9401318311705538 1105 62-63 0.7791284141547905 1105 64-65 1.0159111589159302 1105 66-67 1.0350095485710504 1105 68-69 0.9579157203576472 1105 70-71 0.8590980047805026 1105 72-73 0.862449865491012 1105 74-75 0.905521600680629 1105 76-77 0.899508952200911 1105 78-79 0.9319199118866308 1105 80-81 0.9218192523148439 1105 82-83 1.016406042467711 1105 84-85 0.9374444448553305 1105 86-87 1.0448856582061943 1105 88-89 1.000365985999803 1105 90-91 0.9882197759884654 1105 92-93 1.2657547145187067 1105 94-95 1.262783069717468 1105 96-97 1.2937885377165017 1105 98-99 1.3587158986293204 1105 100-101 1.10339154363996 1106 1 0.34850510268348245 1106 2 0.4554492521624063 1106 3 0.1995605927123556 1106 4 0.41229987136695456 1106 5 0.41093467359631575 1106 6 -0.00511917756909952 1106 7 0.3232491373602855 1106 8 0.22419295421016727 1106 9 0.32446429914416797 1106 10-11 0.16178668296296905 1106 12-13 0.2685939845777625 1106 14-15 0.45817084040463385 1106 16-17 0.32485276091107806 1106 18-19 -0.013747391875448045 1106 20-21 0.2734800570665641 1106 22-23 0.38429733161054 1106 24-25 0.36257538073234485 1106 26-27 0.25485922728183397 1106 28-29 0.28385403050772595 1106 30-31 0.4262149269435582 1106 32-33 0.5160506895192398 1106 34-35 0.4072068661213848 1106 36-37 0.27020378997139716 1106 38-39 0.40906922852718 1106 40-41 0.5552370777693127 1106 42-43 0.026470530257427072 1106 44-45 0.4180619242786605 1106 46-47 0.13177370193916715 1106 48-49 0.5036139609578711 1106 50-51 -0.004871284508489282 1106 52-53 0.30203278048239923 1106 54-55 0.15741692121306272 1106 56-57 -0.17584888170625845 1106 58-59 -0.35065176799017905 1106 60-61 0.1886277989998817 1106 62-63 0.28072028137688676 1106 64-65 0.4680831037747488 1106 66-67 -0.057859954641230615 1106 68-69 -0.0550957719163776 1106 70-71 0.035922707385214636 1106 72-73 0.47021851330740816 1106 74-75 0.34084875152523253 1106 76-77 0.3238828966158991 1106 78-79 0.5919955156591286 1106 80-81 0.5199962514665621 1106 82-83 0.7112437223769454 1106 84-85 0.4396100252977355 1106 86-87 0.6457044243518943 1106 88-89 0.42565277392920464 1106 90-91 0.7752970802963972 1106 92-93 0.7388955036513956 1106 94-95 0.5409756792411393 1106 96-97 0.5881137981615439 1106 98-99 0.4419436925394429 1106 100-101 -0.1003621189519528 1107 1 0.4340571538115974 1107 2 0.5313121285110967 1107 3 0.45774616050745465 1107 4 0.4998403919099559 1107 5 0.46420581535723215 1107 6 0.4336621467353865 1107 7 0.3806287060677249 1107 8 0.42481931054180677 1107 9 0.4654051427557775 1107 10-11 0.4904160000840392 1107 12-13 0.4333585429939504 1107 14-15 0.600978901779591 1107 16-17 0.5225026126697614 1107 18-19 0.4864528679865927 1107 20-21 0.6663909156205321 1107 22-23 0.6950733883165157 1107 24-25 0.590629841034179 1107 26-27 0.5858905093428675 1107 28-29 0.6270297972478076 1107 30-31 0.6386458019681385 1107 32-33 0.5203409218552792 1107 34-35 0.6507211282567766 1107 36-37 0.6721615211554806 1107 38-39 0.6084788872520832 1107 40-41 0.6807853053613968 1107 42-43 0.5692253620131922 1107 44-45 0.6566586461876369 1107 46-47 0.7805382326736492 1107 48-49 0.5795186482208763 1107 50-51 0.647373211877607 1107 52-53 0.6543980094879842 1107 54-55 0.728467505498962 1107 56-57 0.5807171619489537 1107 58-59 0.560150990030742 1107 60-61 0.5890098354163271 1107 62-63 0.6627712595504391 1107 64-65 0.5392245186676199 1107 66-67 0.6019756736787762 1107 68-69 0.5600112611407724 1107 70-71 0.6718629656440243 1107 72-73 0.5523706162233886 1107 74-75 0.6566917143446638 1107 76-77 0.7576189411419243 1107 78-79 0.6851425914614495 1107 80-81 0.5058423919154791 1107 82-83 0.49927449751669073 1107 84-85 0.5116738754658741 1107 86-87 0.5836362662178267 1107 88-89 0.7675328348520303 1107 90-91 1.0236239228457187 1107 92-93 0.9814768056951699 1107 94-95 0.8314196650150443 1107 96-97 0.9632243087333627 1107 98-99 0.833704174247309 1107 100-101 0.5733900559364695 1108 1 0.32536091148934077 1108 2 0.3945877793382202 1108 3 0.4025331163686978 1108 4 0.4074556153453983 1108 5 0.3960196456751959 1108 6 0.31988612241470804 1108 7 0.38830640315411813 1108 8 0.2903138996045129 1108 9 0.33417991310892603 1108 10-11 0.3665420314610941 1108 12-13 0.40387884513270933 1108 14-15 0.47289681589675325 1108 16-17 0.43986547851141466 1108 18-19 0.43179790681464425 1108 20-21 0.4048770666953061 1108 22-23 0.382773674796411 1108 24-25 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2316 52-53 -1.3023949436737183 2316 54-55 -1.172861119363624 2316 56-57 -1.1839685064334233 2316 58-59 -1.1577426101261032 2316 60-61 -1.4333953371881876 2316 62-63 -1.3893448059317919 2316 64-65 -1.5068876744559638 2316 66-67 -1.5045994783814898 2316 68-69 -1.3230165403497338 2316 70-71 -1.5531287762231436 2316 72-73 -1.543639237083184 2316 74-75 -1.3979297885363415 2316 76-77 -1.220225766291307 2316 78-79 -1.2808147149454108 2316 80-81 -1.3265008427856166 2316 82-83 -1.0754727044448167 2316 84-85 -1.2684267326225935 2316 86-87 -1.187176707861937 2316 88-89 -1.3605432910194573 2316 90-91 -1.4032054347592755 2316 92-93 -1.5395399325674077 2316 94-95 -1.6385353024139633 2316 96-97 -1.5943533005505302 2316 98-99 -1.661357195313478 2316 100-101 -1.3156517260198335 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8509.0 3 2669.0 4 2658.0 5 3083.0 6 3656.0 7 4814.0 8 6437.0 9 8266.0 10 11053.0 11 14214.0 12 18366.0 13 22074.0 14 26082.0 15 30966.0 16 35117.0 17 40521.0 18 37945.0 19 34168.0 20 35358.0 21 38028.0 22 42111.0 23 47481.0 24 53541.0 25 59608.0 26 68327.0 27 78290.0 28 89139.0 29 104305.0 30 121222.0 31 143116.0 32 171010.0 33 207120.0 34 258688.0 35 326105.0 36 403125.0 37 427343.0 38 230138.0 39 15314.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88366651406481 19.243932214997415 15.221508425980032 22.65089284495775 2 15.111568997395272 20.479958715893297 40.64607396756435 23.762398319147085 3 18.548742804610004 24.88530120899898 30.6383918504697 25.927564135921322 4 12.525516920932395 17.126182916440797 38.12570278014404 32.22259738248277 5 12.957502662627856 38.07152013591019 34.75362870173426 14.217348499727697 6 30.991725807870708 37.42182146233985 17.522959427089337 14.063493302700111 7 27.598368154988112 33.75628496335101 21.197260353735732 17.448086527925152 8 26.29730468847994 34.62868426912667 20.30773385645384 18.766277185939547 9 26.50851835849118 16.83210912419102 20.620049154203894 36.03932336311391 10-11 24.340344763449604 26.91525312434036 27.9229774622537 20.821424649956334 12-13 25.945046824147113 24.40458565678315 27.90890135770824 21.7414661613615 14-15 22.31941879033167 25.911593346489713 26.574026929695137 25.19496093348348 16-17 21.338320783411216 28.981361944894935 27.707013322939716 21.973303948754136 18-19 21.559904678307873 27.177677577535597 30.172648224673747 21.08976951948278 20-21 22.057831847784843 26.375008711948144 30.382427419791995 21.184732020475014 22-23 21.827926264069415 26.31220452776713 30.44696116125185 21.412908046911603 24-25 21.603840925172968 26.614461280316654 30.424064460722654 21.357633333787724 26-27 20.91836418605804 26.964150744678218 30.15872425841945 21.9587608108443 28-29 21.246657710013512 27.56523321498678 29.91428715236506 21.27382192263465 30-31 21.377874295138366 26.941493328567013 29.605110209096107 22.075522167198518 32-33 20.82251632038286 27.098882547451076 29.679833969628213 22.39876716253785 34-35 22.02716796541651 27.15644254158689 29.35371849678521 21.46267099621139 36-37 22.28614226966055 26.362277834814524 29.427332301767535 21.92424759375739 38-39 21.45822026674553 26.91314860220665 29.381090595466397 22.247540535581425 40-41 22.01687282209059 26.838796187550468 29.489332743912307 21.654998246446635 42-43 21.77224401509011 26.840699942014712 29.068914235194203 22.31814180770097 44-45 21.642620652052926 27.0685979024492 29.495300736311634 21.793480709186237 46-47 22.316140668663834 27.434662455586263 28.473282088200456 21.775914787549443 48-49 21.86116604569174 27.606414914049697 28.183421533137704 22.348997507120853 50-51 22.147739219836822 27.6565984053172 28.388639026798558 21.807023348047423 52-53 22.519224957704473 27.156824132353414 28.477816213243763 21.84613469669835 54-55 21.63435844814037 27.566074296001204 28.695169279872378 22.104397975986046 56-57 21.984069396015816 27.662747153717742 28.27430635488702 22.078877095379426 58-59 21.93644133593611 27.009730679717077 28.37552159604636 22.678306388300456 60-61 21.245392266904055 27.54562836242598 28.86333552657042 22.34564384409955 62-63 21.537119721050594 27.497365962884153 28.986901180339665 21.978613135725595 64-65 21.14981391690972 27.54918352560887 28.66105321171364 22.63994934576777 66-67 21.092815353863607 27.557145387128497 28.555355201137395 22.7946840578705 68-69 21.939951379377558 27.037205783386348 28.717021506180373 22.30582133105572 70-71 21.757757853852045 27.05583610941007 28.18273100919647 23.00367502754142 72-73 21.52762864592857 27.266605838935742 28.377437050962605 22.82832846417308 74-75 21.604074938210303 27.792628775179022 28.18943297472626 22.413863311884416 76-77 21.737218079859822 27.15604841075666 28.43885016944198 22.667883339941536 78-79 21.727165768778253 27.491485540900367 28.20691683695585 22.574431853365535 80-81 22.19330526405091 27.480113377319242 27.808142217972836 22.518439140657016 82-83 22.033091722487026 27.439914260427294 28.084865209880363 22.442128807205318 84-85 21.715232079242007 27.738109023401435 28.109633345945046 22.43702555141151 86-87 22.13026688480816 27.744845664066226 27.547846430698996 22.577041020426613 88-89 21.80225819593629 27.623329065233488 27.474567539389593 23.09984519944063 90-91 21.779215682995172 27.511133493208405 27.68059425707181 23.029056566724616 92-93 22.13537658679518 27.068829742331683 27.876298026556807 22.91949564431634 94-95 21.575073492156502 26.79913320755325 28.24087093005826 23.38492237023199 96-97 21.95642798352635 26.285259892049428 28.289739895022674 23.46857222940155 98-99 21.65732374706687 26.71505733876593 27.693931063420628 23.93368785074657 100-101 22.033108102348226 26.06790718075852 28.122450845836767 23.776533871056486 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 470.0 1 350.0 2 321.5 3 401.5 4 356.5 5 383.5 6 662.0 7 1517.5 8 2551.0 9 2973.0 10 2708.5 11 2321.0 12 2390.5 13 2632.0 14 2868.5 15 3099.5 16 3354.5 17 3787.0 18 4127.0 19 4661.5 20 5545.5 21 6504.0 22 7762.5 23 9565.0 24 11791.5 25 14946.0 26 19228.5 27 24231.5 28 29866.5 29 36030.0 30 42785.0 31 52493.5 32 67474.0 33 74160.0 34 72350.0 35 76308.0 36 84553.0 37 92052.5 38 97145.0 39 100048.5 40 103081.0 41 107470.5 42 109281.5 43 110693.0 44 112319.0 45 117628.0 46 120305.0 47 121633.5 48 123673.5 49 117939.0 50 110909.5 51 104676.5 52 99617.5 53 99307.5 54 100937.0 55 95775.0 56 86390.5 57 77330.0 58 71322.5 59 61790.5 60 46875.0 61 38404.0 62 34405.5 63 26059.0 64 16993.0 65 12132.0 66 8631.5 67 6375.5 68 4993.0 69 3980.5 70 3225.0 71 2314.0 72 1773.5 73 1495.5 74 1136.0 75 866.5 76 606.0 77 382.0 78 262.5 79 164.5 80 102.5 81 58.0 82 34.5 83 17.5 84 12.0 85 10.5 86 9.0 87 6.5 88 3.0 89 2.0 90 2.0 91 1.0 92 0.5 93 2.0 94 3.0 95 3.0 96 3.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18241666790506902 2 0.04975281488856856 3 0.04681160927910122 4 0.053189381442788286 5 0.060495955377886095 6 0.0650161239987517 7 0.038080872627840275 8 0.03526350725456104 9 0.04188895989062431 10-11 0.04354532304964012 12-13 0.0400003962887558 14-15 0.03455142589647947 16-17 0.032926022796510676 18-19 0.04092919806016654 20-21 0.050867377014261445 22-23 0.04815837184764679 24-25 0.06823597013964225 26-27 0.053498982033258535 28-29 0.04506236594294433 30-31 0.054598064129427915 32-33 0.050867377014261445 34-35 0.04975281488856856 36-37 0.047755891080035466 38-39 0.02664113080996468 40-41 0.025093127857613452 42-43 0.021687521362440742 44-45 0.004752369063718278 46-47 0.012167303205480673 48-49 0.011424261788352083 50-51 0.009210617566489823 52-53 0.00965953842267168 54-55 0.010712180430270515 56-57 0.008436616090314207 58-59 0.010449019928370808 60-61 0.010619300253129443 62-63 0.004179607971348323 64-65 0.007058893462721613 66-67 0.004210568030395347 68-69 0.008312775854126108 70-71 0.02292592372432173 72-73 0.019489357170101997 74-75 0.020665839413888932 76-77 0.01227566341214526 78-79 0.005201289919900136 80-81 0.006857653078915952 82-83 0.005805011071317115 84-85 0.005139369801806086 86-87 0.0080496153522264 88-89 0.030170577541325485 90-91 0.037059190679288465 92-93 0.02287948363575119 94-95 0.03256998211746989 96-97 0.03639354940977743 98-99 0.021532721067205617 100-101 0.12447491739856247 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3229968.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.9302734681576 #Duplication Level Percentage of deduplicated Percentage of total 1 68.37840810981753 29.35503759471752 2 15.129497298734002 12.99026912940805 3 6.384155041558101 8.222205653916188 4 3.269761673403479 5.61487051259648 5 1.8608211181066685 3.9942779737821037 6 1.193325993459795 3.0737886741533913 7 0.7782483497927704 2.3387290137922205 8 0.5646382722902037 1.9392060352005185 9 0.4091680922755415 1.580912828625001 >10 1.8222775328027851 13.667969212435327 >50 0.12967807891593067 3.820017734931633 >100 0.06840264238835703 5.548817834672745 >500 0.006389788050684577 1.8281871083555163 >1k 0.004719729809378098 4.500579700852609 >5k 4.356673670195168E-4 1.1730410084831797 >10k+ 7.261122783658613E-5 0.3520899840774233 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11295 0.349693866936143 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8781 0.2718602784919231 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6017 0.1862866752859471 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5812 0.17993986318130706 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5773 0.1787324208784731 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 5667 0.1754506546194885 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5581 0.17278808954144437 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4970 0.15387149346371234 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4863 0.1505587671456807 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4392 0.1359765793345321 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 4197 0.1299393678203623 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4183 0.12950592699370397 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 4012 0.12421175689666275 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 3945 0.12213743294051212 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3847 0.1191033471539037 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3407 0.10548092117321287 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3378 0.10458307946084915 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3342 0.10346851733515625 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3292 0.10192051438280504 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.192011809404923E-5 0.0 3 0.0 0.0 0.0 6.192011809404923E-5 0.0 4 0.0 0.0 0.0 1.2384023618809846E-4 0.0 5 0.0 0.0 0.0 1.2384023618809846E-4 0.0 6 0.0 0.0 0.0 1.2384023618809846E-4 0.0 7 0.0 0.0 0.0 1.2384023618809846E-4 0.0 8 0.0 0.0 0.0 1.2384023618809846E-4 0.0 9 0.0 0.0 0.0 1.2384023618809846E-4 0.0 10-11 0.0 0.0 0.0 1.2384023618809846E-4 0.0 12-13 0.0 0.0 0.0 1.2384023618809846E-4 3.0960059047024616E-5 14-15 0.0 0.0 0.0 1.3932026571161077E-4 4.644008857053693E-5 16-17 0.0 0.0 0.0 2.0124038380566E-4 6.192011809404923E-5 18-19 0.0 0.0 0.0 2.167204133291723E-4 6.192011809404923E-5 20-21 0.0 0.0 0.0 2.4768047237619693E-4 1.7028032475863538E-4 22-23 0.0 0.0 0.0 6.346812104640046E-4 2.167204133291723E-4 24-25 0.0 0.0 0.0 0.0011145621256928862 2.167204133291723E-4 26-27 0.0 0.0 0.0 0.0018730835723449893 2.167204133291723E-4 28-29 0.0 0.0 0.0 0.004520168620865594 2.167204133291723E-4 30-31 0.0 0.0 0.0 0.015278789139706647 2.167204133291723E-4 32-33 0.0 0.0 0.0 0.038901314192586425 2.167204133291723E-4 34-35 0.0 0.0 0.0 0.07450538209666474 2.167204133291723E-4 36-37 0.0 0.0 0.0 0.13171957121556624 2.167204133291723E-4 38-39 0.0 0.0 0.0 0.2216121026586022 2.7864053142322154E-4 40-41 0.0 0.0 0.0 0.31817652682627195 2.7864053142322154E-4 42-43 0.0 0.0 0.0 0.4189515190243371 2.7864053142322154E-4 44-45 0.0 0.0 0.0 0.5461664016485612 3.0960059047024615E-4 46-47 0.0 0.0 0.0 0.695486766432361 3.4056064951727076E-4 48-49 0.0 0.0 0.0 0.8486771385970386 3.7152070856429537E-4 50-51 0.0 0.0 0.0 1.0235240720651102 3.7152070856429537E-4 52-53 0.0 0.0 0.0 1.194144957473263 4.0248076761132E-4 54-55 0.0 0.0 0.0 1.3774285070316488 4.0248076761132E-4 56-57 0.0 0.0 0.0 1.5902479529208957 4.0248076761132E-4 58-59 0.0 0.0 0.0 1.8170458654698747 4.0248076761132E-4 60-61 0.0 1.5480029523512308E-5 0.0 2.0658408999717643 4.0248076761132E-4 62-63 0.0 3.0960059047024616E-5 0.0 2.3130414914327324 4.0248076761132E-4 64-65 0.0 3.0960059047024616E-5 0.0 2.5679666176259333 4.0248076761132E-4 66-67 0.0 3.0960059047024616E-5 0.0 2.870074873806799 4.0248076761132E-4 68-69 0.0 3.0960059047024616E-5 0.0 3.203468269654684 4.0248076761132E-4 70-71 0.0 3.0960059047024616E-5 0.0 3.5566606232631406 4.0248076761132E-4 72-73 0.0 3.0960059047024616E-5 0.0 3.924729285243693 4.0248076761132E-4 74-75 0.0 3.0960059047024616E-5 0.0 4.295708192774665 4.0248076761132E-4 76-77 0.0 3.0960059047024616E-5 0.0 4.693544951528931 4.0248076761132E-4 78-79 0.0 3.0960059047024616E-5 0.0 5.146227454885002 4.0248076761132E-4 80-81 0.0 3.0960059047024616E-5 0.0 5.61048902032466 4.0248076761132E-4 82-83 0.0 3.0960059047024616E-5 0.0 6.097862269842921 4.644008857053692E-4 84-85 0.0 3.0960059047024616E-5 0.0 6.595436858817177 4.644008857053692E-4 86-87 0.0 3.0960059047024616E-5 0.0 7.1086772376692275 4.644008857053692E-4 88-89 0.0 3.0960059047024616E-5 0.0 7.644735180038936 4.644008857053692E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9460 0.0 37.398727 1 GTATCAA 14970 0.0 36.133972 1 ATCAACG 18495 0.0 29.086792 3 TCAACGC 18615 0.0 28.899284 4 CAACGCA 18655 0.0 28.836426 5 TATCAAC 18770 0.0 28.761913 2 AACGCAG 19240 0.0 28.052788 6 TAAGGTG 2405 0.0 26.477682 5 ACGCAGA 21420 0.0 24.817589 7 GTACATG 12230 0.0 24.606497 1 CGCAGAG 21670 0.0 24.531656 8 GGTAAGG 2290 0.0 24.487827 3 TACATGG 12225 0.0 24.257132 2 GTAAGGT 2515 0.0 23.430817 4 ACATGGG 12340 0.0 23.337868 3 AGGTAAG 2340 0.0 23.152224 2 GCAGAGT 22585 0.0 22.612265 9 GAGTACT 12165 0.0 22.297325 12-13 GTACTTT 12440 0.0 21.957165 14-15 GCGCGAC 130 2.9402607E-4 21.926212 9 >>END_MODULE