FastQCFastQC Report
Thu 26 May 2016
SRR938518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938518_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3229968
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104970.3249877398166174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86120.26662802851297596No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC62330.1929740480401044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60790.18820619894686264No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA59410.18393371079837323No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA57950.17941354217750763No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA54940.17009456440435325No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA50110.15514085588464033No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG49310.15266405116087836No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48990.15167332927137359No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG41240.12767928350992952No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT40000.12384023618809846No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA39040.12086807051958409No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38690.11978446845293823No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA37930.11743150396536436No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT36070.11167293298261778No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG35930.11123949215595944No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT35160.10885556760933854No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35040.10848404690077425No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT33170.10269451585898065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91850.039.35151
GTATCAA142450.034.3542021
TAAGGTG27100.027.7157295
AGGTAAG24400.027.4824982
ATCAACG179600.026.9984653
GGTAAGG26650.026.5787323
TATCAAC184400.026.4614352
TCAACGC183700.026.4472314
CAACGCA183800.026.4069795
GTAAGGT27700.026.0856884
AACGCAG188700.025.6985686
GTACATG124500.024.218481
AAGGTAA28100.023.5253141
TACATGG123900.023.337762
GTGTAAG12900.022.857511
ACGCAGA212600.022.5861917
CGCAGAG214400.022.3522628
ACATGGG126600.022.079483
GAGTACT127150.021.38524612-13
AGAGTAC187000.020.63653810-11
Produced by FastQC (version 0.11.5)