##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938518_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3229968 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.832175427124973 31.0 31.0 34.0 30.0 34.0 2 32.06142011314044 33.0 31.0 34.0 30.0 34.0 3 32.11866309511426 33.0 31.0 34.0 30.0 34.0 4 35.793169467932806 37.0 35.0 37.0 35.0 37.0 5 35.65847401584164 37.0 35.0 37.0 33.0 37.0 6 35.68861920613455 37.0 35.0 37.0 33.0 37.0 7 35.63312330029276 37.0 35.0 37.0 33.0 37.0 8 35.616417871632166 37.0 35.0 37.0 33.0 37.0 9 37.20583207016293 39.0 37.0 39.0 33.0 39.0 10-11 37.12038246199343 39.0 37.0 39.0 33.0 39.0 12-13 37.0258993277952 39.0 37.0 39.0 33.0 39.0 14-15 38.08425284708703 40.0 37.0 41.0 33.0 41.0 16-17 38.02299078504802 40.0 37.0 41.0 33.0 41.0 18-19 38.14751384533841 40.0 37.5 41.0 33.0 41.0 20-21 38.19649807676113 40.0 37.5 41.0 33.0 41.0 22-23 38.110558680457515 40.0 37.0 41.0 33.0 41.0 24-25 38.01342567480545 40.0 37.0 41.0 33.0 41.0 26-27 37.83268115968951 40.0 37.0 41.0 32.5 41.0 28-29 37.72009304736146 40.0 37.0 41.0 32.0 41.0 30-31 37.472652515442874 39.0 36.5 41.0 32.0 41.0 32-33 37.236536863523106 39.0 36.0 41.0 31.0 41.0 34-35 37.08319339386644 39.0 36.0 41.0 31.0 41.0 36-37 37.05077341323505 39.0 36.0 41.0 31.0 41.0 38-39 36.91730103827654 39.0 36.0 41.0 31.0 41.0 40-41 36.74250828491181 39.0 35.0 41.0 30.0 41.0 42-43 36.528484492725624 39.0 35.0 41.0 30.0 41.0 44-45 36.264742715717304 39.0 35.0 40.5 30.0 41.0 46-47 35.90996783869066 39.0 35.0 40.0 28.5 41.0 48-49 35.549224636281224 38.0 35.0 40.0 27.5 41.0 50-51 35.194225298826495 38.0 34.0 40.0 26.5 41.0 52-53 34.73440356065447 38.0 34.0 40.0 26.0 41.0 54-55 34.44554744814809 38.0 33.0 40.0 24.5 41.0 56-57 34.1313449545011 37.5 33.0 40.0 24.0 41.0 58-59 33.71386357388061 37.0 33.0 40.0 23.0 41.0 60-61 33.37361778816384 36.5 32.0 39.5 22.5 41.0 62-63 32.94902689438409 36.0 31.5 39.0 21.5 40.0 64-65 32.48795963303661 35.5 31.0 39.0 20.0 40.0 66-67 32.02961345127878 35.0 31.0 38.5 18.5 40.0 68-69 31.52900539571909 35.0 30.5 38.0 17.5 40.0 70-71 30.973786272805178 34.5 29.5 37.0 13.5 39.5 72-73 30.570625467496892 34.0 29.0 37.0 11.0 39.0 74-75 30.103194056411706 34.0 29.0 36.0 8.0 39.0 76-77 28.79865466159417 32.5 27.0 35.0 7.0 37.0 78-79 29.517599709966166 34.0 29.0 35.0 7.0 37.0 80-81 29.284642912870964 34.0 29.0 35.0 3.5 37.0 82-83 28.846971084543252 34.0 28.5 35.0 2.0 36.5 84-85 28.36528488827134 33.0 27.5 35.0 2.0 36.0 86-87 27.86576445958598 33.0 26.5 35.0 2.0 36.0 88-89 27.27148194657037 32.5 26.0 35.0 2.0 35.0 90-91 26.783508691107777 32.0 25.0 34.5 2.0 35.0 92-93 26.133817734417185 32.0 24.5 34.0 2.0 35.0 94-95 25.45357724906253 32.0 21.5 34.0 2.0 35.0 96-97 24.561725688923232 31.0 18.0 34.0 2.0 35.0 98-99 23.44554884135075 31.0 2.0 34.0 2.0 35.0 100-101 19.787769414433825 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3462726289301621 1101 2 0.3604273979607129 1101 3 0.37311943419511095 1101 4 0.19461804191332988 1101 5 0.1286341480673272 1101 6 0.1893429351594449 1101 7 0.3333170547414923 1101 8 0.2940963379705721 1101 9 0.3637489829139753 1101 10-11 0.43477932868562164 1101 12-13 0.46566372777426324 1101 14-15 0.5729171800159278 1101 16-17 0.5498738280862909 1101 18-19 0.5519029123264687 1101 20-21 0.4739630078395223 1101 22-23 0.4886423388159429 1101 24-25 0.5424135292118919 1101 26-27 0.5220880278861486 1101 28-29 0.5629711307702863 1101 30-31 0.527061242206976 1101 32-33 0.5035158064282541 1101 34-35 0.419896276370217 1101 36-37 0.6614517620754867 1101 38-39 0.5672710087641448 1101 40-41 0.6257606907049578 1101 42-43 0.6398235311980258 1101 44-45 0.6809020580744729 1101 46-47 0.6498474543458741 1101 48-49 0.6420377814277956 1101 50-51 0.601892220265583 1101 52-53 0.6788401203424641 1101 54-55 0.7098205921824672 1101 56-57 0.6842118536137036 1101 58-59 0.8136856554163998 1101 60-61 0.699228437157025 1101 62-63 0.7608620850065435 1101 64-65 0.873381225684021 1101 66-67 0.6071905076147104 1101 68-69 0.5821837617667569 1101 70-71 0.6026245090331663 1101 72-73 0.5733554542056716 1101 74-75 0.6977561256309635 1101 76-77 0.748461621966559 1101 78-79 0.5740403103502238 1101 80-81 0.6469902132471752 1101 82-83 0.4983174229599676 1101 84-85 0.6486205504418621 1101 86-87 0.5422088642344391 1101 88-89 0.554689203862754 1101 90-91 0.6120612502471268 1101 92-93 0.6991219951820931 1101 94-95 0.7111366674336885 1101 96-97 0.7253978129620897 1101 98-99 0.8005415212730433 1101 100-101 0.7201778610677785 1102 1 0.3396295829317957 1102 2 0.27161283088527455 1102 3 0.3202083897173509 1102 4 0.2709607466293491 1102 5 0.24410098729727991 1102 6 0.258040424021857 1102 7 0.2669437243126893 1102 8 0.296560917646552 1102 9 0.42689048908396643 1102 10-11 0.41496952205047677 1102 12-13 0.4061275236540993 1102 14-15 0.5177938335694918 1102 16-17 0.5319994853431353 1102 18-19 0.518640430319607 1102 20-21 0.4772167228623516 1102 22-23 0.4362259722129167 1102 24-25 0.4603026081766828 1102 26-27 0.3772120150378697 1102 28-29 0.34149933461707604 1102 30-31 0.32090577293306666 1102 32-33 0.32574651799554744 1102 34-35 0.3698011316348939 1102 36-37 0.4886660929442428 1102 38-39 0.48169093555288356 1102 40-41 0.6111643563934805 1102 42-43 0.5272513450688692 1102 44-45 0.5573337674334624 1102 46-47 0.42858602309568994 1102 48-49 0.5376145656852387 1102 50-51 0.5520101100386441 1102 52-53 0.6245702467322616 1102 54-55 0.5275044318865483 1102 56-57 0.4118947536166857 1102 58-59 0.44790834108211897 1102 60-61 0.5680589791509831 1102 62-63 0.6378389277817362 1102 64-65 0.5222899444015852 1102 66-67 0.5367972854223808 1102 68-69 0.4650129134302432 1102 70-71 0.4841634404221722 1102 72-73 0.4804884261812248 1102 74-75 0.4557620051698308 1102 76-77 0.5167577623579014 1102 78-79 0.5111961982182578 1102 80-81 0.5066933744870674 1102 82-83 0.4828836334444695 1102 84-85 0.3842153598747551 1102 86-87 0.21226112442912637 1102 88-89 0.3505909324856198 1102 90-91 0.26260083088416053 1102 92-93 0.29351390179289183 1102 94-95 0.23531047796432247 1102 96-97 0.3036014995803207 1102 98-99 0.37545999152509424 1102 100-101 0.2829944762644523 1103 1 0.25504009834230956 1103 2 0.2983416576141025 1103 3 0.25453239904135927 1103 4 0.1624979131665114 1103 5 0.21140220459849957 1103 6 0.15837712435855167 1103 7 0.2440157263846885 1103 8 0.1462760173616502 1103 9 0.21570167789515438 1103 10-11 0.3219916665726146 1103 12-13 0.2714376764642523 1103 14-15 0.4374551907308515 1103 16-17 0.3740578899015361 1103 18-19 0.3822800314592101 1103 20-21 0.35133612198175257 1103 22-23 0.35312897661592757 1103 24-25 0.4019466123206854 1103 26-27 0.45047995080580705 1103 28-29 0.4271733827911177 1103 30-31 0.5007879278152174 1103 32-33 0.5482115529605807 1103 34-35 0.42994099177475675 1103 36-37 0.5356001773783277 1103 38-39 0.6049750588370131 1103 40-41 0.6515813468104668 1103 42-43 0.5763027566202794 1103 44-45 0.6689337415334435 1103 46-47 0.5966156786253407 1103 48-49 0.6546244076950813 1103 50-51 0.7739021069306418 1103 52-53 0.7820747792367939 1103 54-55 0.6534789203610387 1103 56-57 0.709521663743601 1103 58-59 0.6963187269925015 1103 60-61 0.6470152081072129 1103 62-63 0.4356018130446202 1103 64-65 0.5858388954505429 1103 66-67 0.6242746228997191 1103 68-69 0.485567821485148 1103 70-71 0.44204352330225305 1103 72-73 0.4131807338735314 1103 74-75 0.4779615648693891 1103 76-77 0.39522512832526857 1103 78-79 0.44261292963498633 1103 80-81 0.5332862260799196 1103 82-83 0.39015830321913825 1103 84-85 0.18379448445388036 1103 86-87 0.3677066048746056 1103 88-89 0.33518689208158037 1103 90-91 0.19191966020298779 1103 92-93 0.25246883574782686 1103 94-95 0.26994528759913194 1103 96-97 0.1992600077388289 1103 98-99 0.18928089284599636 1103 100-101 -0.3500701443001688 1104 1 0.264886159103515 1104 2 0.22758720408094746 1104 3 0.23658387501961897 1104 4 0.12356455035627789 1104 5 0.09168099402870666 1104 6 0.1506981976280457 1104 7 0.11744869940169167 1104 8 0.164802831645531 1104 9 0.23361005903149135 1104 10-11 0.2507267002577436 1104 12-13 0.37024211661644557 1104 14-15 0.43092644493421517 1104 16-17 0.43287972530407615 1104 18-19 0.45749441773848787 1104 20-21 0.314371796789473 1104 22-23 0.3688862226976255 1104 24-25 0.4188688747504372 1104 26-27 0.5017776132099669 1104 28-29 0.5039495600248713 1104 30-31 0.4699407383989751 1104 32-33 0.4435517784588825 1104 34-35 0.4850737541870487 1104 36-37 0.4972613946967783 1104 38-39 0.580562948484804 1104 40-41 0.5420087230947459 1104 42-43 0.5986454721410368 1104 44-45 0.6176285159769677 1104 46-47 0.6536677363845556 1104 48-49 0.6239494254393989 1104 50-51 0.6157685183893946 1104 52-53 0.6359500221303378 1104 54-55 0.5705323743945243 1104 56-57 0.584193830179494 1104 58-59 0.7339468642462634 1104 60-61 0.6154736631297268 1104 62-63 0.5339323926148012 1104 64-65 0.6388856822743705 1104 66-67 0.63691875912955 1104 68-69 0.5089431981883052 1104 70-71 0.4520617912240148 1104 72-73 0.22086984177062519 1104 74-75 0.298496396094734 1104 76-77 0.19745393113643317 1104 78-79 0.3139532365826163 1104 80-81 0.2937210491603288 1104 82-83 0.17748682328476306 1104 84-85 0.21346705813643396 1104 86-87 0.3662230634576211 1104 88-89 0.34279978667450806 1104 90-91 0.24130180883638275 1104 92-93 0.3943803352300428 1104 94-95 0.48983783265806835 1104 96-97 0.5387347177210238 1104 98-99 0.5223624119924075 1104 100-101 0.23130006618817944 1105 1 0.19069323803003257 1105 2 0.23882261437747587 1105 3 0.17183342974296778 1105 4 0.12447827990192195 1105 5 0.15160897189460343 1105 6 0.17859470696807023 1105 7 0.2009209569210455 1105 8 0.21596369439802743 1105 9 0.2607164453883115 1105 10-11 0.240083749001883 1105 12-13 0.3106597576171666 1105 14-15 0.4649502700991306 1105 16-17 0.30431787167448476 1105 18-19 0.32538084675022816 1105 20-21 0.3200864158761334 1105 22-23 0.3474794308366924 1105 24-25 0.384122057752613 1105 26-27 0.34713173801955577 1105 28-29 0.3531483284346564 1105 30-31 0.47454769350581927 1105 32-33 0.45918422582086293 1105 34-35 0.41495926360858704 1105 36-37 0.38896792714376716 1105 38-39 0.43742670901200853 1105 40-41 0.3959476433381468 1105 42-43 0.5536615809531682 1105 44-45 0.510894302586216 1105 46-47 0.30203066869451334 1105 48-49 0.3147591176857034 1105 50-51 0.20804843610579127 1105 52-53 0.19104253258495163 1105 54-55 0.272999497446456 1105 56-57 0.40245845797722524 1105 58-59 0.27263257484813863 1105 60-61 0.2459215291316923 1105 62-63 0.43042361788083383 1105 64-65 0.25120414179561834 1105 66-67 0.1984381714869059 1105 68-69 0.2865381767782722 1105 70-71 0.2893998173444281 1105 72-73 0.22184905082714934 1105 74-75 0.19229235895522478 1105 76-77 0.2660234462417428 1105 78-79 0.30478306817400025 1105 80-81 0.4317007638316319 1105 82-83 0.391857684529473 1105 84-85 0.4147631155666005 1105 86-87 0.47933564186387656 1105 88-89 0.36857782871458866 1105 90-91 0.3387500792236082 1105 92-93 0.4348074065590204 1105 94-95 0.40473583326864215 1105 96-97 0.3055889253789843 1105 98-99 0.13546177728336417 1105 100-101 -0.10986875107283822 1106 1 0.09472669074577666 1106 2 0.09558234193772108 1106 3 0.1507215768797252 1106 4 0.0356874976525674 1106 5 -0.024340127426007996 1106 6 -0.040721251050591434 1106 7 0.026032898916639624 1106 8 0.09502452020929297 1106 9 -0.0858689154771568 1106 10-11 0.04667768122526894 1106 12-13 0.0757842514018634 1106 14-15 0.17115095159534377 1106 16-17 0.04622735532647937 1106 18-19 0.2368191529668735 1106 20-21 0.12543005315384903 1106 22-23 0.19557027379983083 1106 24-25 0.20606186472002008 1106 26-27 0.1928495846673428 1106 28-29 0.21696324815295753 1106 30-31 0.2369182417338962 1106 32-33 0.13668825738103862 1106 34-35 -0.012544081144064023 1106 36-37 -0.2775644304955307 1106 38-39 -0.08045684322978275 1106 40-41 0.04108472554168685 1106 42-43 0.11575935975657359 1106 44-45 0.05869427493076529 1106 46-47 -0.07481482128466155 1106 48-49 0.081812472252075 1106 50-51 0.1825903125921613 1106 52-53 0.04564247941078037 1106 54-55 -0.23231379820098397 1106 56-57 0.04028745022912261 1106 58-59 0.09129768353007961 1106 60-61 -0.1910539597760632 1106 62-63 -0.14344833128198076 1106 64-65 -0.4954757543102488 1106 66-67 -0.08987843884628788 1106 68-69 -0.8149893747345871 1106 70-71 -0.5487246852924557 1106 72-73 -0.32061641114859185 1106 74-75 -0.01306446502699643 1106 76-77 -0.11253662057089642 1106 78-79 -0.17470870646643633 1106 80-81 -0.8325134810448596 1106 82-83 -0.22306902395003547 1106 84-85 -0.12265354797127515 1106 86-87 2.976312722324792E-5 1106 88-89 0.030039410075186623 1106 90-91 0.15390621194168475 1106 92-93 -0.057613138204498426 1106 94-95 0.19761482968716848 1106 96-97 0.19948983034710466 1106 98-99 0.14652349930214115 1106 100-101 -0.44439529801521616 1107 1 0.1608456055997216 1107 2 0.20364394758063753 1107 3 0.20156047683168765 1107 4 0.14595816132730732 1107 5 0.17632083104162888 1107 6 0.18629182913351627 1107 7 0.056836873779339214 1107 8 0.17693123631087815 1107 9 0.24067893898856596 1107 10-11 0.19932139975534824 1107 12-13 0.1831701061640132 1107 14-15 0.2750200759363892 1107 16-17 0.30494694942036205 1107 18-19 0.2851349805029102 1107 20-21 0.3151674056406222 1107 22-23 0.25548589072419503 1107 24-25 0.3224281271760603 1107 26-27 0.3270704051185902 1107 28-29 0.37473630081684206 1107 30-31 0.30748353106254456 1107 32-33 0.3209097410398982 1107 34-35 0.3588478532133834 1107 36-37 0.40158827276751197 1107 38-39 0.3668712086642003 1107 40-41 0.42198100160214125 1107 42-43 0.4970495234650514 1107 44-45 0.4944853979834605 1107 46-47 0.49826863849064296 1107 48-49 0.50409030779619 1107 50-51 0.5855582819715721 1107 52-53 0.684103935803158 1107 54-55 0.5473689232800822 1107 56-57 0.6275461075393665 1107 58-59 0.4470005769030294 1107 60-61 0.346822403331835 1107 62-63 0.32635236618174446 1107 64-65 0.5261436602562171 1107 66-67 0.44562984057980515 1107 68-69 0.6301959398882246 1107 70-71 0.5495424974074012 1107 72-73 0.44500515567072796 1107 74-75 0.39400010890248893 1107 76-77 0.3842161131420241 1107 78-79 0.486021819595603 1107 80-81 0.5093090538268861 1107 82-83 0.46236829226215903 1107 84-85 0.5998521714734544 1107 86-87 0.6059270051639736 1107 88-89 0.5410216955116631 1107 90-91 0.6106639225951653 1107 92-93 0.3576184048719888 1107 94-95 0.3070952670812339 1107 96-97 0.6364258515743622 1107 98-99 0.470269157454954 1107 100-101 0.03940311686093523 1108 1 0.16330805623880096 1108 2 0.14419572924825985 1108 3 0.06989883052963108 1108 4 0.13751999361206657 1108 5 0.10799506196401865 1108 6 0.03575999408911912 1108 7 0.1517605076064399 1108 8 0.18099427087633302 1108 9 0.1721528047771912 1108 10-11 0.2343626907935743 1108 12-13 0.15070335877599916 1108 14-15 0.3423534128653287 1108 16-17 0.25458995070849255 1108 18-19 0.2268508395515667 1108 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-0.33919378823730284 2316 80-81 -0.4855547689905855 2316 82-83 -0.38624029817946237 2316 84-85 -0.40211059407415206 2316 86-87 -0.26099911563439093 2316 88-89 -0.13083683828641313 2316 90-91 -0.24068953388161418 2316 92-93 -0.26491050622167833 2316 94-95 -0.25747360498369076 2316 96-97 -0.35523099306364614 2316 98-99 -0.13589106634235648 2316 100-101 -0.09019395491577953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 23.0 4 27.0 5 83.0 6 204.0 7 570.0 8 1367.0 9 2608.0 10 4385.0 11 6703.0 12 9591.0 13 13105.0 14 16806.0 15 21831.0 16 27145.0 17 33316.0 18 31412.0 19 25748.0 20 24925.0 21 25265.0 22 27471.0 23 31117.0 24 36054.0 25 42569.0 26 51696.0 27 62549.0 28 75366.0 29 90514.0 30 109202.0 31 131503.0 32 158917.0 33 198225.0 34 255867.0 35 339625.0 36 463587.0 37 565979.0 38 325966.0 39 18641.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.61344592971271 19.878425136782557 15.772038152983459 22.736090780521273 2 15.390665220297652 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21.980666681527495 26.42530823834787 30.30971204668281 21.28431303344182 22-23 21.817847730999194 26.34940965359409 30.420997978927346 21.411744636479373 24-25 21.681752518144197 26.740471547927246 30.23903097191976 21.338744962008793 26-27 21.006567193755437 27.074347692812623 30.023020785276604 21.896064328155333 28-29 21.29635649641111 27.536635037870344 29.840311111441352 21.326697354277194 30-31 21.45731784339659 26.933904608342868 29.537444333813834 22.071333214446707 32-33 20.872915768824953 27.152838665893903 29.544998588221304 22.429246977059837 34-35 22.06414062957198 27.221914477715597 29.247201890270315 21.466743002442104 36-37 22.279888834691526 26.423432827202898 29.37727334154911 21.919404996556462 38-39 21.470582634936054 26.901153324991316 29.410971549479843 22.21729249059279 40-41 22.007722485896135 26.855732680989377 29.460964787483025 21.675580045631467 42-43 21.82185003930398 26.874075257441827 29.037218188623797 22.266856514630398 44-45 21.647877006831028 27.103519291831997 29.461220668440056 21.78738303289692 46-47 22.37743531824464 27.41412608422127 28.4600497590069 21.748388838527195 48-49 21.928220341501834 27.643911642468282 28.118157826950608 22.309710189079272 50-51 22.14357851223294 27.59439102802257 28.449167298251872 21.812863161492622 52-53 22.59647773600234 27.168055534915514 28.433315748019794 21.80215098106235 54-55 21.69218085132732 27.58338472703135 28.617682899644826 22.106751521996504 56-57 22.013221183615443 27.64375684217305 28.173622772733353 22.169399201478157 58-59 21.891579111619684 27.09333343240552 28.325961743274235 22.68912571270056 60-61 21.24694424217206 27.6081063341804 28.761538814006826 22.383410609640713 62-63 21.5566067527604 27.607610973235648 28.788938466263442 22.04684380774051 64-65 21.172841340843004 27.607812213619454 28.51500386381537 22.704342581722173 66-67 21.072661944117343 27.70914355250654 28.36249438654821 22.855700116827908 68-69 21.906636626883667 27.16175135196052 28.512132891673808 22.419479129482013 70-71 21.748923246679645 27.20579436708979 28.010679374339876 23.034603011890688 72-73 21.509470062861304 27.455117202399528 28.145557479207223 22.889855255531945 74-75 21.57561622901527 27.932087872077986 27.882102856746567 22.61019304216017 76-77 21.578975395421875 27.282979274098075 28.36354725495732 22.77449807552273 78-79 21.625864404848592 27.608168254298494 28.134969139013144 22.63099820183977 80-81 22.005079674290307 27.602055956914256 27.793066199701137 22.5997981690943 82-83 21.86096919267381 27.575878977543276 28.013579793594072 22.549572036188838 84-85 21.576734258138906 27.81071931478025 28.003734601194623 22.608811825886217 86-87 21.900831947579654 27.830645058919945 27.49111347720403 22.77740951629637 88-89 21.518514906508273 27.865038785249997 27.306565241419023 23.309881066822705 90-91 21.472693226761052 27.658398604520695 27.676077400306763 23.19283076841149 92-93 21.683990773204645 27.332840961002226 27.80857727874554 23.174590987047594 94-95 21.175486057747978 27.014278168872362 28.125172215435086 23.68506355794457 96-97 21.422568493336232 26.547976101926725 28.265485643431397 23.76396976130565 98-99 21.127141895611285 27.06031580518185 27.652257020662134 24.160285278544727 100-101 21.14347899785369 26.669726552976154 28.196258940685937 23.990535508484218 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 455.0 1 321.5 2 288.0 3 399.0 4 373.0 5 391.5 6 674.5 7 1504.5 8 2471.5 9 2901.5 10 2585.0 11 2156.0 12 2232.0 13 2422.0 14 2660.5 15 2889.5 16 3167.0 17 3520.5 18 3891.0 19 4393.5 20 5095.5 21 6153.5 22 7394.0 23 9016.5 24 11282.5 25 14295.5 26 18390.0 27 23477.5 28 28931.5 29 35402.5 30 42612.5 31 52146.5 32 68072.0 33 75057.5 34 72568.0 35 76733.5 36 84877.5 37 91837.5 38 96612.0 39 100074.0 40 103849.5 41 108378.0 42 110325.5 43 111493.5 44 113725.5 45 119341.5 46 121174.5 47 122692.5 48 125273.0 49 119224.5 50 111871.0 51 106100.0 52 100874.0 53 100602.0 54 102731.5 55 96204.0 56 86071.5 57 76896.5 58 70460.0 59 61121.5 60 46203.5 61 37991.5 62 34042.5 63 24829.5 64 15307.5 65 10881.0 66 7775.5 67 5809.5 68 4585.0 69 3721.5 70 2903.5 71 2039.5 72 1586.0 73 1353.5 74 1022.5 75 717.5 76 506.0 77 333.5 78 209.0 79 127.0 80 69.0 81 24.0 82 9.0 83 6.0 84 2.5 85 1.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.430414171285907E-4 2 0.051207937663778715 3 0.0012693624209280093 4 0.0 5 0.08836000852020826 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.0960059047024615E-4 26-27 0.008606896415072843 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011671942260728279 36-37 1.0836020666458616E-4 38-39 0.004102207823730761 40-41 0.005541850569417406 42-43 4.644008857053692E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.0836020666458616E-4 68-69 8.978417123637139E-4 70-71 1.0836020666458616E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0011300421552163983 82-83 6.811212990345415E-4 84-85 0.005325130156088234 86-87 0.002383924546620895 88-89 9.288017714107384E-5 90-91 0.0034056064951727078 92-93 8.514016237931769E-4 94-95 6.192011809404923E-5 96-97 1.5480029523512308E-5 98-99 6.192011809404923E-5 100-101 1.5480029523512308E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3229968.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.832605609622405 #Duplication Level Percentage of deduplicated Percentage of total 1 66.46600068368119 27.139799923656472 2 15.743264787699571 12.856770441679846 3 6.774022506826361 8.298029682358393 4 3.534876818243676 5.773529239917637 5 2.042968952654927 4.170987275823099 6 1.2810175566523518 3.1384370801872565 7 0.8467609189564164 2.420281825457215 8 0.5987315849647831 1.955821653991289 9 0.4407437708547949 1.6197044913190461 >10 2.0420544054887446 14.53199317730868 >50 0.13820341383638768 3.891395224158376 >100 0.0783857163527089 5.960179698134127 >500 0.0073566182895627875 2.040142863515876 >1k 0.00500553409331904 4.541120984452948 >5k 5.308899795944436E-4 1.3367350247472465 >10k+ 7.584142565634908E-5 0.3250714132925251 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10497 0.3249877398166174 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8612 0.26662802851297596 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6233 0.1929740480401044 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6079 0.18820619894686264 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 5941 0.18393371079837323 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5795 0.17941354217750763 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5494 0.17009456440435325 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 5011 0.15514085588464033 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4931 0.15266405116087836 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4899 0.15167332927137359 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 4124 0.12767928350992952 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4000 0.12384023618809846 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3904 0.12086807051958409 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3869 0.11978446845293823 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3793 0.11743150396536436 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3607 0.11167293298261778 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3593 0.11123949215595944 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3516 0.10885556760933854 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3504 0.10848404690077425 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3317 0.10269451585898065 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.0960059047024616E-5 7 0.0 0.0 0.0 0.0 3.0960059047024616E-5 8 0.0 0.0 0.0 0.0 3.0960059047024616E-5 9 0.0 0.0 0.0 0.0 3.0960059047024616E-5 10-11 0.0 0.0 0.0 1.5480029523512308E-5 3.0960059047024616E-5 12-13 0.0 0.0 0.0 3.0960059047024616E-5 7.740014761756154E-5 14-15 0.0 0.0 0.0 3.0960059047024616E-5 1.3932026571161077E-4 16-17 0.0 0.0 0.0 2.167204133291723E-4 1.8576035428214768E-4 18-19 0.0 0.0 0.0 2.167204133291723E-4 2.167204133291723E-4 20-21 0.0 0.0 0.0 2.322004428526846E-4 2.4768047237619693E-4 22-23 0.0 0.0 0.0 6.192011809404923E-4 2.4768047237619693E-4 24-25 0.0 0.0 0.0 0.0011300421552163983 2.4768047237619693E-4 26-27 0.0 0.0 0.0 0.0019040436313920138 2.7864053142322154E-4 28-29 0.0 0.0 0.0 0.004597568768483155 3.0960059047024615E-4 30-31 0.0 0.0 0.0 0.015263309110183136 3.0960059047024615E-4 32-33 0.0 0.0 0.0 0.03905611448782155 3.0960059047024615E-4 34-35 0.0 0.0 0.0 0.07501622307094064 3.4056064951727076E-4 36-37 0.0 0.0 0.0 0.13263289295745345 3.4056064951727076E-4 38-39 0.0 0.0 0.0 0.22303626537476534 3.4056064951727076E-4 40-41 0.0 0.0 0.0 0.3203592109890872 3.4056064951727076E-4 42-43 0.0 0.0 0.0 0.42178436442713985 3.4056064951727076E-4 44-45 0.0 0.0 0.0 0.5496184482323045 3.4056064951727076E-4 46-47 0.0 0.0 0.0 0.700053375141797 3.4056064951727076E-4 48-49 0.0 0.0 0.0 0.8536926681626567 3.4056064951727076E-4 50-51 0.0 0.0 0.0 1.028895642309769 3.4056064951727076E-4 52-53 0.0 0.0 0.0 1.2003214892531444 3.7152070856429537E-4 54-55 0.0 0.0 0.0 1.384363560258182 3.7152070856429537E-4 56-57 0.0 0.0 0.0 1.5983440083616927 4.179607971348323E-4 58-59 0.0 0.0 0.0 1.826426763361123 4.334408266583446E-4 60-61 0.0 0.0 0.0 2.076769800815364 4.334408266583446E-4 62-63 0.0 0.0 0.0 2.3255957953763007 4.953609447523939E-4 64-65 0.0 0.0 0.0 2.5819296042561413 4.953609447523939E-4 66-67 0.0 0.0 0.0 2.8864063049541047 5.108409742759061E-4 68-69 0.0 0.0 0.0 3.2217811445809987 5.263210037994184E-4 70-71 0.0 0.0 0.0 3.5796484671055566 5.263210037994184E-4 72-73 0.0 0.0 0.0 3.9518812570279334 5.263210037994184E-4 74-75 0.0 0.0 0.0 4.327937614242618 5.263210037994184E-4 76-77 0.0 0.0 0.0 4.733545347817687 5.263210037994184E-4 78-79 0.0 0.0 0.0 5.194138146260272 5.263210037994184E-4 80-81 0.0 0.0 0.0 5.667563889177849 5.263210037994184E-4 82-83 0.0 0.0 0.0 6.1660827599530394 5.572810628464431E-4 84-85 0.0 0.0 0.0 6.676118772693723 5.572810628464431E-4 86-87 0.0 0.0 0.0 7.203585298677881 5.727610923699554E-4 88-89 0.0 0.0 0.0 7.7573214347634405 5.882411218934676E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9185 0.0 39.3515 1 GTATCAA 14245 0.0 34.354202 1 TAAGGTG 2710 0.0 27.715729 5 AGGTAAG 2440 0.0 27.482498 2 ATCAACG 17960 0.0 26.998465 3 GGTAAGG 2665 0.0 26.578732 3 TATCAAC 18440 0.0 26.461435 2 TCAACGC 18370 0.0 26.447231 4 CAACGCA 18380 0.0 26.406979 5 GTAAGGT 2770 0.0 26.085688 4 AACGCAG 18870 0.0 25.698568 6 GTACATG 12450 0.0 24.21848 1 AAGGTAA 2810 0.0 23.525314 1 TACATGG 12390 0.0 23.33776 2 GTGTAAG 1290 0.0 22.85751 1 ACGCAGA 21260 0.0 22.586191 7 CGCAGAG 21440 0.0 22.352262 8 ACATGGG 12660 0.0 22.07948 3 GAGTACT 12715 0.0 21.385246 12-13 AGAGTAC 18700 0.0 20.636538 10-11 >>END_MODULE