FastQCFastQC Report
Thu 26 May 2016
SRR938517_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938517_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3220788
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115030.3571486232561721No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87610.27201417789683763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61560.19113334997522344No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA58580.18188095584062036No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC57770.17936604334094639No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA57720.1792108018286208No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA55960.1737463005947613No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48940.15195039226425336No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA48830.1516088609371371No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG44080.1368609172662094No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA42340.13145851263728006No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT41370.12844682729816428No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA39720.12332385739142097No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG39560.1228270845519792No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA38120.11835612899700322No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34430.10689930538737724No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT33470.10391866835072659No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT32670.10143480415351772No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT32390.10056545168449461No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98600.036.4091951
GTATCAA157100.035.627471
ATCAACG197000.028.08333
TCAACGC198450.027.8541554
TATCAAC198650.027.8496062
CAACGCA198400.027.7893075
TAAGGTG26700.027.7699225
AACGCAG204300.027.0295226
GTGTAAG14650.027.0105511
TAAGGTA13450.026.5033254
AGGTAAG24400.025.906962
GTAAGGT27700.025.223134
GGTAAGG27450.025.1065483
ACGCAGA225100.024.2702667
GTACATG123900.024.0877381
TACATGG123200.023.8800132
CGCAGAG227700.023.8679528
ATACCGA1000.001649707223.7613246
ACATGGG127500.022.5903933
AAGGTAA27800.022.2941821
Produced by FastQC (version 0.11.5)