FastQCFastQC Report
Thu 26 May 2016
SRR938517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938517_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3220788
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105380.32718701137733996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87420.27142426015000054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62860.1951696292956879No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC62240.193244634542851No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA61370.19054343222838632No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA58610.1819741007480157No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA55070.1709830016753664No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA49170.1526645032209509No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG48710.1512362813075558No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA48160.14952862467197467No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG41950.13024762884114074No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA39420.12239240831746764No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT39380.12226821510760721No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA38090.11826298408960788No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA37770.11726943841072433No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT36210.11242590322616702No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT36050.11192913038672525No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA35660.1107182465905859No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34400.10680616047998191No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT32430.10068964489435504No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94650.035.731981
GTATCAA148650.034.879451
ATCAACG188850.027.1624873
TATCAAC190500.027.0671422
TCAACGC190950.026.888664
CAACGCA192300.026.6504525
AACGCAG196900.026.074346
GTGTAAG15150.026.0599481
TAAGGTA14050.026.0543044
TAAGGTG26500.023.8600225
ACGCAGA217300.023.495367
CGCAGAG218200.023.3766848
GGTAAGG25300.023.3005523
AGGTAAG24450.022.9567932
GTACATG120050.022.2284281
TGTAAGG17250.022.0601752
GTAAGGT28600.021.775614
TACATGG118650.021.6488132
GAGTACT127050.021.62529612-13
AGAGTAC189300.021.61414310-11
Produced by FastQC (version 0.11.5)