##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938517_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3220788 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.839733009437442 31.0 31.0 34.0 30.0 34.0 2 32.074392043189434 33.0 31.0 34.0 30.0 34.0 3 32.137498028432795 33.0 31.0 34.0 30.0 34.0 4 35.80866142074548 37.0 35.0 37.0 35.0 37.0 5 35.68019844833004 37.0 35.0 37.0 33.0 37.0 6 35.718370473312746 37.0 35.0 37.0 33.0 37.0 7 35.65489035602467 37.0 35.0 37.0 33.0 37.0 8 35.63289449662629 37.0 35.0 37.0 33.0 37.0 9 37.21291187125635 39.0 37.0 39.0 33.0 39.0 10-11 37.152270500262674 39.0 37.0 39.0 33.0 39.0 12-13 37.05913661501471 39.0 37.0 39.0 33.0 39.0 14-15 38.111136001500256 40.0 37.0 41.0 33.0 41.0 16-17 38.046813078041765 40.0 37.0 41.0 33.0 41.0 18-19 38.076101717964676 40.0 37.0 41.0 33.0 41.0 20-21 38.070984336752375 40.0 37.0 41.0 33.0 41.0 22-23 38.098594971168545 40.0 37.0 41.0 33.0 41.0 24-25 38.02422248840967 40.0 37.0 41.0 33.0 41.0 26-27 37.85241375713024 40.0 37.0 41.0 32.5 41.0 28-29 37.7362260415774 40.0 37.0 41.0 32.0 41.0 30-31 37.48430880890018 39.5 36.5 41.0 32.0 41.0 32-33 37.25205881293646 39.0 36.0 41.0 31.0 41.0 34-35 37.07440011574808 39.0 36.0 41.0 31.0 41.0 36-37 37.0587031807123 39.0 36.0 41.0 31.0 41.0 38-39 36.902280280477946 39.0 36.0 41.0 31.0 41.0 40-41 36.72976877087223 39.0 35.0 41.0 30.0 41.0 42-43 36.49871848131575 39.0 35.0 41.0 30.0 41.0 44-45 36.22362819285218 39.0 35.0 40.0 30.0 41.0 46-47 35.874336342534804 38.5 35.0 40.0 28.5 41.0 48-49 35.48004277214147 38.0 35.0 40.0 27.5 41.0 50-51 35.12278020161526 38.0 34.0 40.0 26.5 41.0 52-53 34.63958152476971 38.0 33.5 40.0 25.5 41.0 54-55 34.348427155093724 38.0 33.0 40.0 24.0 41.0 56-57 34.016212647339714 37.5 33.0 40.0 23.5 41.0 58-59 33.59112863063325 37.0 32.5 40.0 23.0 41.0 60-61 33.210300864260546 36.5 32.0 39.0 22.0 41.0 62-63 32.77505085711944 36.0 31.5 39.0 20.5 40.0 64-65 32.313542679617534 35.5 31.0 39.0 19.0 40.0 66-67 31.85817275151298 35.0 31.0 38.0 18.0 40.0 68-69 31.33432579232163 35.0 30.0 38.0 14.5 40.0 70-71 30.789503841916947 34.0 29.0 37.0 10.0 39.5 72-73 30.24874766672007 34.0 29.0 36.5 8.0 39.0 74-75 29.682154180902316 34.0 28.5 36.0 7.0 39.0 76-77 28.37423217548004 32.5 27.0 34.5 4.0 37.0 78-79 29.037001969704306 33.5 28.0 35.0 2.0 37.0 80-81 28.78911480668706 34.0 28.0 35.0 2.0 37.0 82-83 28.332873507973822 33.0 27.0 35.0 2.0 36.5 84-85 27.78099086310555 33.0 26.0 35.0 2.0 36.0 86-87 27.208057779648954 32.5 25.5 35.0 2.0 35.5 88-89 26.500738173391106 32.0 24.5 34.5 2.0 35.0 90-91 26.0131165106179 32.0 23.5 34.0 2.0 35.0 92-93 25.31408354104648 31.5 20.5 34.0 2.0 35.0 94-95 24.59695763893805 31.0 18.0 34.0 2.0 35.0 96-97 23.7023112045872 31.0 4.5 34.0 2.0 35.0 98-99 22.59688560687633 31.0 2.0 34.0 2.0 35.0 100-101 19.388815097423365 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3369970594502618 1101 2 0.3460958902801039 1101 3 0.2830359759347445 1101 4 0.25404097005669257 1101 5 0.25939669863657855 1101 6 0.25720320585044476 1101 7 0.30045118622361855 1101 8 0.338613064796391 1101 9 0.3560297992799306 1101 10-11 0.3748236338198083 1101 12-13 0.4398649107607824 1101 14-15 0.5240609349350365 1101 16-17 0.48025060065833713 1101 18-19 0.5628700035242673 1101 20-21 0.5306461993396425 1101 22-23 0.5695030030253534 1101 24-25 0.587869989416248 1101 26-27 0.552949773520595 1101 28-29 0.5907727518526613 1101 30-31 0.5722879706773725 1101 32-33 0.5386475675483453 1101 34-35 0.4585418398716641 1101 36-37 0.7010248645672306 1101 38-39 0.6912694788997129 1101 40-41 0.8326396104108014 1101 42-43 0.7735332081699156 1101 44-45 0.8139420792098164 1101 46-47 0.9294354706261885 1101 48-49 0.8945786297557916 1101 50-51 0.9510309114254341 1101 52-53 0.9459232072497556 1101 54-55 0.9975646372823377 1101 56-57 1.014471727380375 1101 58-59 1.0787996190461868 1101 60-61 1.233228763168448 1101 62-63 1.2713650291133902 1101 64-65 1.1982188921753547 1101 66-67 1.2838620003752155 1101 68-69 1.3101973827240307 1101 70-71 1.4744632364132855 1101 72-73 1.576582795279844 1101 74-75 1.5523378023187675 1101 76-77 1.2624904747970156 1101 78-79 1.572276803971615 1101 80-81 1.8612992403848345 1101 82-83 1.8092862289638454 1101 84-85 1.9805468593997517 1101 86-87 1.9983043117062387 1101 88-89 2.0469168940066425 1101 90-91 2.3922504710059656 1101 92-93 2.6499734111251207 1101 94-95 2.584159184456734 1101 96-97 3.0757198926029936 1101 98-99 3.2010591832608384 1101 100-101 3.941933227572502 1102 1 0.32661805191367677 1102 2 0.3130474912199688 1102 3 0.2857668842146097 1102 4 0.27738984683126944 1102 5 0.13527652829844072 1102 6 0.21316893074709498 1102 7 0.2841461336542821 1102 8 0.28726265269636286 1102 9 0.3741710181001139 1102 10-11 0.3363380560239335 1102 12-13 0.3121320573895474 1102 14-15 0.5084820498095439 1102 16-17 0.48398647281136675 1102 18-19 0.5223537503407982 1102 20-21 0.5527385470908541 1102 22-23 0.5362899195627762 1102 24-25 0.5229454330267558 1102 26-27 0.5885981113751839 1102 28-29 0.46204660515646623 1102 30-31 0.4154565504494627 1102 32-33 0.33896059758574637 1102 34-35 0.48067049599507783 1102 36-37 0.5440910028606325 1102 38-39 0.7555793864170965 1102 40-41 0.6529818946223642 1102 42-43 0.586284449138212 1102 44-45 0.5354218528530552 1102 46-47 0.47777228783428427 1102 48-49 0.5781472759203297 1102 50-51 0.7041949267164469 1102 52-53 0.8034258136721846 1102 54-55 0.7050073348906523 1102 56-57 0.8442710458203564 1102 58-59 0.8259377412809101 1102 60-61 0.8654469972329579 1102 62-63 0.9489220462250074 1102 64-65 0.8509876649005079 1102 66-67 0.8478140110809989 1102 68-69 1.0387157936621954 1102 70-71 1.0413743794527122 1102 72-73 1.0533233927081689 1102 74-75 1.203730747083359 1102 76-77 1.0989770173582123 1102 78-79 1.393974168502485 1102 80-81 1.5366777551649342 1102 82-83 1.527113121019056 1102 84-85 1.6694113409939995 1102 86-87 1.7672482048973812 1102 88-89 1.8458450189904312 1102 90-91 1.958100890886353 1102 92-93 2.0663720874256093 1102 94-95 2.3271166569790758 1102 96-97 2.7158909569051204 1102 98-99 2.9590664760567407 1102 100-101 3.8996663921583554 1103 1 0.21950108757572195 1103 2 0.25233080995037227 1103 3 0.2542083533273356 1103 4 0.14591812993578657 1103 5 0.07299508572462798 1103 6 0.17456257029751043 1103 7 0.21748004457564463 1103 8 0.12856707749779162 1103 9 0.2959408633341525 1103 10-11 0.3210854001862913 1103 12-13 0.3447797183966017 1103 14-15 0.5058973433862377 1103 16-17 0.5227669258262182 1103 18-19 0.45403946360048764 1103 20-21 0.3486207588110233 1103 22-23 0.3600357311078639 1103 24-25 0.2686467341316856 1103 26-27 0.29287260179814467 1103 28-29 0.2356769242085548 1103 30-31 0.42066915683552253 1103 32-33 0.34401317441939483 1103 34-35 0.4514656991013979 1103 36-37 0.4662845591379252 1103 38-39 0.5218647947605319 1103 40-41 0.5091723324265516 1103 42-43 0.5398430443135425 1103 44-45 0.6624911561337328 1103 46-47 0.6208344385661633 1103 48-49 0.7955915267472307 1103 50-51 0.7764282737863297 1103 52-53 0.8726543845913284 1103 54-55 0.903434562691622 1103 56-57 0.7917311275817056 1103 58-59 1.0637268397840316 1103 60-61 1.113273382810135 1103 62-63 1.0394370588679038 1103 64-65 0.940013379370626 1103 66-67 0.9673435932563308 1103 68-69 1.040436817640387 1103 70-71 0.8964773822324155 1103 72-73 1.0440289340625952 1103 74-75 1.1017160195931552 1103 76-77 1.0550325506891838 1103 78-79 1.3111821983823724 1103 80-81 1.5511560663578159 1103 82-83 1.8065047550833917 1103 84-85 1.9161979425589664 1103 86-87 1.8683872587127617 1103 88-89 2.176533313064102 1103 90-91 2.245981938892207 1103 92-93 2.332472981506765 1103 94-95 2.3322669843082586 1103 96-97 2.6823093611700273 1103 98-99 2.9910692474747727 1103 100-101 3.4759076978890064 1104 1 0.36862054115270126 1104 2 0.2754509941302672 1104 3 0.23020571126329514 1104 4 0.18857344677108756 1104 5 0.17650601503454766 1104 6 0.16629762479539067 1104 7 0.24065332489778513 1104 8 0.286206338059678 1104 9 0.28873677875412085 1104 10-11 0.28833569964853467 1104 12-13 0.3871456612566604 1104 14-15 0.5288757608663346 1104 16-17 0.5575894997572846 1104 18-19 0.6354652649274684 1104 20-21 0.532474646722946 1104 22-23 0.5183094905695924 1104 24-25 0.5299970964597591 1104 26-27 0.4787967082962652 1104 28-29 0.4892184368964081 1104 30-31 0.5804673065411592 1104 32-33 0.5501403630423809 1104 34-35 0.6204664721812279 1104 36-37 0.6050454863239949 1104 38-39 0.5842171874850877 1104 40-41 0.6744590303562248 1104 42-43 0.6446728817968719 1104 44-45 0.7578789066413307 1104 46-47 0.74147152855776 1104 48-49 0.732900911596559 1104 50-51 0.6714667578702276 1104 52-53 0.7846422192032207 1104 54-55 0.8391776113446809 1104 56-57 0.855975866815939 1104 58-59 1.0113851937186666 1104 60-61 0.888198304865 1104 62-63 0.9588737512755827 1104 64-65 0.9737177485459725 1104 66-67 1.0443526450866614 1104 68-69 1.1399140330238176 1104 70-71 1.2440680916818252 1104 72-73 1.2958766798127819 1104 74-75 1.337189446035886 1104 76-77 1.246908203771735 1104 78-79 1.6529429514130989 1104 80-81 1.742405715923276 1104 82-83 1.8189796937042466 1104 84-85 2.0274311747972433 1104 86-87 2.181770247558326 1104 88-89 2.1609442857004737 1104 90-91 2.443252160027022 1104 92-93 2.624002660126436 1104 94-95 2.898375398135343 1104 96-97 3.3304141474686766 1104 98-99 3.6219793790682466 1104 100-101 4.125353692740806 1105 1 0.31791115170586437 1105 2 0.32844796527645315 1105 3 0.2865623189470199 1105 4 0.24476665576199963 1105 5 0.18453646298608817 1105 6 0.2014921090931736 1105 7 0.30297597753607874 1105 8 0.28428142175169313 1105 9 0.3618596864537835 1105 10-11 0.40063444028299955 1105 12-13 0.4548505074227691 1105 14-15 0.6048979990298946 1105 16-17 0.6136790900649487 1105 18-19 0.5516955375243171 1105 20-21 0.5132892489866165 1105 22-23 0.4233330040400247 1105 24-25 0.4060153492893406 1105 26-27 0.4947719195189606 1105 28-29 0.5507838760802102 1105 30-31 0.5950775408787976 1105 32-33 0.5597988199210633 1105 34-35 0.6110770253455797 1105 36-37 0.640887587392065 1105 38-39 0.585693753721344 1105 40-41 0.6904342415789273 1105 42-43 0.764320578273832 1105 44-45 0.712392297363472 1105 46-47 0.8450005417890694 1105 48-49 0.7892256366085135 1105 50-51 0.7726782993974055 1105 52-53 0.9180592445499443 1105 54-55 0.8462117258034709 1105 56-57 0.8974014207773635 1105 58-59 1.088336014697461 1105 60-61 1.104564078816857 1105 62-63 1.0665873651764315 1105 64-65 1.18821240819846 1105 66-67 1.369502095110576 1105 68-69 1.3789005625949962 1105 70-71 1.4361412624135426 1105 72-73 1.4898548401826197 1105 74-75 1.699681475367946 1105 76-77 1.3965814063861117 1105 78-79 1.6522654446380294 1105 80-81 1.9507729245953591 1105 82-83 1.9660126125628494 1105 84-85 1.9562331834055655 1105 86-87 2.2487517556087013 1105 88-89 2.533708513342752 1105 90-91 2.593192380017456 1105 92-93 2.9316744280205853 1105 94-95 2.9444199541691383 1105 96-97 3.2256576513743056 1105 98-99 3.5719654303993487 1105 100-101 4.125052800025994 1106 1 0.09233240407778354 1106 2 0.17643447170698323 1106 3 0.06978390865817374 1106 4 0.10831293448854495 1106 5 0.05250610245477816 1106 6 0.08219630474993522 1106 7 0.11417985693729804 1106 8 0.04019750861654359 1106 9 0.08202203096148253 1106 10-11 0.20740336290580785 1106 12-13 0.09746277813750481 1106 14-15 0.020796164747793 1106 16-17 0.08111952860596006 1106 18-19 0.1948928809778252 1106 20-21 0.3061165734647915 1106 22-23 0.2099733160788162 1106 24-25 0.2984115120555231 1106 26-27 0.13588408481519565 1106 28-29 0.2246179473431269 1106 30-31 0.27067842639429784 1106 32-33 0.22276318846417809 1106 34-35 0.2298204366886054 1106 36-37 0.3184271193338688 1106 38-39 0.21528262152652644 1106 40-41 0.25724533160633456 1106 42-43 0.3580481768401427 1106 44-45 -0.11090835623129891 1106 46-47 -0.262219572062925 1106 48-49 0.19765700915753825 1106 50-51 -0.027336459194195584 1106 52-53 0.2852572205145236 1106 54-55 0.38204980296981716 1106 56-57 0.2507990594027021 1106 58-59 0.6957922660151965 1106 60-61 0.448782927646711 1106 62-63 0.5793914975820798 1106 64-65 0.34578567406394 1106 66-67 0.4516874208537729 1106 68-69 0.4226924664533165 1106 70-71 0.6343133054242927 1106 72-73 0.906088236767058 1106 74-75 1.1871219139089533 1106 76-77 1.1634494224097232 1106 78-79 1.2712977027025438 1106 80-81 1.1928696987889076 1106 82-83 1.5371047483418927 1106 84-85 1.554688802212226 1106 86-87 1.6929189493679253 1106 88-89 1.9942999111922148 1106 90-91 2.150518966566054 1106 92-93 1.881094626207389 1106 94-95 2.1713881177786654 1106 96-97 2.8747268059178417 1106 98-99 3.4462675598532826 1106 100-101 3.9861354481390023 1107 1 0.15582848079966993 1107 2 0.16337272961298055 1107 3 0.2044787648046409 1107 4 0.15393986640579982 1107 5 0.09917369379979135 1107 6 0.10619012261471994 1107 7 0.07404075889498074 1107 8 0.13946120046088595 1107 9 0.18617357253237543 1107 10-11 0.10249867082369946 1107 12-13 0.20843785145128635 1107 14-15 0.36486210752340753 1107 16-17 0.29968039886038156 1107 18-19 0.3059085740902887 1107 20-21 0.3504658641044074 1107 22-23 0.2916426907320613 1107 24-25 0.32663811108857743 1107 26-27 0.25931239582414634 1107 28-29 0.2518863154179485 1107 30-31 0.346713735897346 1107 32-33 0.33833959331477814 1107 34-35 0.35278845602760356 1107 36-37 0.4269519271328761 1107 38-39 0.49381872361101387 1107 40-41 0.4929558808652743 1107 42-43 0.4326627841160402 1107 44-45 0.5389626511397054 1107 46-47 0.6636972465151842 1107 48-49 0.736238090238615 1107 50-51 0.6308096928229148 1107 52-53 0.7760252319276901 1107 54-55 0.6332143032472217 1107 56-57 0.575859691882691 1107 58-59 0.7042790299051873 1107 60-61 0.7195469761526923 1107 62-63 0.8582319509386593 1107 64-65 0.8444279820610845 1107 66-67 1.0832751596189354 1107 68-69 1.1072018585433518 1107 70-71 1.0815139077861815 1107 72-73 1.1502772278108893 1107 74-75 1.0648904008709863 1107 76-77 1.0388649332284636 1107 78-79 1.2109263782989608 1107 80-81 1.3964731702956072 1107 82-83 1.520954118239647 1107 84-85 1.7917033460816079 1107 86-87 1.8136318763711756 1107 88-89 2.0061081857424234 1107 90-91 2.070738709769664 1107 92-93 2.3266046523625725 1107 94-95 2.484206933661998 1107 96-97 2.736164548645906 1107 98-99 2.9419897996884252 1107 100-101 3.283005940332078 1108 1 0.14361310313082498 1108 2 0.12801080552790722 1108 3 0.13277490103634193 1108 4 0.0738395339188358 1108 5 0.06202839054470388 1108 6 0.0030839789357841596 1108 7 0.06202842110698015 1108 8 0.13156097000695155 1108 9 0.15487098007226763 1108 10-11 0.1265684256512145 1108 12-13 0.24575614175287086 1108 14-15 0.3987301858425667 1108 16-17 0.39726415097873513 1108 18-19 0.26243714920477856 1108 20-21 0.36644627552037434 1108 22-23 0.3431481178341471 1108 24-25 0.29526808300017393 1108 26-27 0.324669980573141 1108 28-29 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44-45 -0.7585146315349434 2316 46-47 -0.7444924922666587 2316 48-49 -0.6934006831501591 2316 50-51 -0.6408895805210904 2316 52-53 -0.5828273752801749 2316 54-55 -0.45700530999870637 2316 56-57 -0.327034622195832 2316 58-59 -0.5144725243726853 2316 60-61 -0.24608141217306212 2316 62-63 -0.24455897257594472 2316 64-65 -0.15737466941261857 2316 66-67 -0.08621107072256251 2316 68-69 -0.1064525968874186 2316 70-71 -0.16713977672276314 2316 72-73 -0.0047138071143564275 2316 74-75 0.2434713327413931 2316 76-77 0.15643361987609694 2316 78-79 0.2048970235853993 2316 80-81 0.2800745440285617 2316 82-83 0.46692337835364484 2316 84-85 0.6379100376830351 2316 86-87 0.6034400411793364 2316 88-89 0.7907766644898508 2316 90-91 0.8956182221491815 2316 92-93 0.8009351316272664 2316 94-95 1.2771904881587126 2316 96-97 1.5184462782960573 2316 98-99 1.800851110852367 2316 100-101 2.4902586360716157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 56.0 5 79.0 6 200.0 7 520.0 8 1446.0 9 2569.0 10 4276.0 11 6647.0 12 9665.0 13 13233.0 14 16919.0 15 22251.0 16 27953.0 17 34609.0 18 32435.0 19 27423.0 20 26670.0 21 27890.0 22 30418.0 23 34353.0 24 39840.0 25 46391.0 26 54929.0 27 66448.0 28 80193.0 29 96313.0 30 114448.0 31 137578.0 32 166641.0 33 205735.0 34 260026.0 35 337967.0 36 439891.0 37 518525.0 38 314099.0 39 22135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.62531032778314 19.843994699433804 15.811751658289836 22.718943314493224 2 15.370154484325896 21.105827339001042 39.56222037709545 23.961797799577607 3 18.531163258589533 24.939952433882475 30.944243319941133 25.58464098758686 4 12.86734799061596 17.69405499523719 37.6151115813894 31.823485432757447 5 12.975815003549304 38.19399465180145 34.81015884224306 14.02003150240619 6 31.14517316880217 37.66727272953079 17.16042782076933 14.027126280897717 7 27.619234795956764 33.66818927541956 21.07782319109485 17.634752737528828 8 26.453246845181987 34.8082208453335 20.068970699096 18.669561610388513 9 26.238734123450534 16.69762182422438 20.46846920691458 36.5951748454105 10-11 24.35380720494488 26.982635926363365 27.846974094538357 20.816582774153407 12-13 25.82459634101965 24.3467126678316 27.915978946767066 21.912712044381685 14-15 22.134024344352994 25.935392208366398 26.485894135224054 25.44468931205655 16-17 21.346484152325456 29.007559640684207 27.675339078511225 21.970617128479116 18-19 21.55481826186635 27.286303848623376 30.031346366168776 21.127531523341492 20-21 21.97122257037719 26.378591201904626 30.341829390819886 21.308356836898298 22-23 21.81371453197168 26.377768421889304 30.38326335045958 21.42525369567944 24-25 21.71058800443871 26.72935998296053 30.21487488349016 21.345177129110603 26-27 21.032604704463825 27.07708445152705 29.993980579177897 21.896330264831228 28-29 21.326100941757108 27.512444159628018 29.837170282551973 21.3242846160629 30-31 21.43380750300858 26.90377323810198 29.53584650712807 22.12657275176137 32-33 20.86333839060239 27.15919592682075 29.55274915847157 22.42471652410529 34-35 22.101060658380803 27.179743648346843 29.245179898294055 21.4740157949783 36-37 22.291322407710656 26.41190417196833 29.363775295076888 21.932998125244126 38-39 21.509571060603 26.898926681126632 29.37547839776921 22.216023860501153 40-41 22.03026407123128 26.862930153422482 29.440796087254 21.66600968809224 42-43 21.846256406785177 26.866083402238523 29.051850131450298 22.23581005952601 44-45 21.67644743372658 27.06643899299265 29.47235089110522 21.78476268217555 46-47 22.365034130638588 27.437288997578698 28.41094869219056 21.786728179592156 48-49 21.95745264823391 27.619219271805534 28.106367137483122 22.316960942477433 50-51 22.157760771587572 27.61436021246974 28.396560096473284 21.831318919469396 52-53 22.619526649999937 27.135222808828154 28.41504936059126 21.83020118058065 54-55 21.668439524737423 27.57946192049896 28.634995535254106 22.117103019509514 56-57 22.021551868673132 27.65366736339057 28.19011993338276 22.134660834553532 58-59 21.88891352054218 27.14646229432052 28.27545619270812 22.689167992429184 60-61 21.253261624173962 27.618582781605 28.74577587844962 22.38237971577142 62-63 21.573369622589254 27.61069651277886 28.778500789247847 22.037433075384037 64-65 21.14251232928091 27.660342748420575 28.521281127475635 22.67586379482288 66-67 21.099919041413074 27.705115138945274 28.356059435866904 22.838906383774752 68-69 21.91810530515488 27.158462268097892 28.49655324443672 22.42687918231051 70-71 21.761072135142083 27.212144978185464 27.985915248069727 23.040867638602727 72-73 21.51537473366374 27.46513081039963 28.154931053352634 22.864563402583997 74-75 21.61477253392648 27.94207504498899 27.815863695468312 22.627288725616214 76-77 21.610875971967108 27.265703299937776 28.36063721052115 22.762783517573958 78-79 21.645727691484197 27.580595183538936 28.173943767798438 22.59973335717843 80-81 22.032258269835644 27.583169514651157 27.790075498639847 22.594496716873348 82-83 21.903116475210464 27.584845039391396 28.00412574198464 22.5079127434135 84-85 21.583868915454353 27.81932405339437 28.036703275343804 22.560103755807464 86-87 21.920667896405142 27.841172476254673 27.503454952419304 22.73470467492088 88-89 21.524305646652238 27.913090739228284 27.309076406695326 23.253527207424156 90-91 21.453541172799547 27.703457729631715 27.68485960589986 23.15814149166888 92-93 21.690542375333656 27.349114825397724 27.866070827008514 23.09427197226011 94-95 21.20532304516783 27.04887747967268 28.117668719580426 23.62813075557907 96-97 21.48289486920592 26.571960029657337 28.257820756907936 23.687324344228802 98-99 21.159526799031788 27.03170776840947 27.692555362228127 24.116210070330617 100-101 21.11907299013526 26.64698500107428 28.21768375453774 24.01625825425272 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 476.0 1 331.0 2 269.5 3 366.0 4 354.5 5 394.5 6 677.0 7 1598.5 8 2613.0 9 2919.5 10 2560.0 11 2172.5 12 2257.5 13 2428.0 14 2637.0 15 2938.5 16 3266.0 17 3604.5 18 3957.5 19 4473.5 20 5167.0 21 6057.5 22 7298.0 23 9018.0 24 11277.0 25 14277.5 26 18435.5 27 23595.5 28 29194.0 29 35490.0 30 42626.5 31 52712.0 32 68210.0 33 74359.0 34 71781.5 35 75783.5 36 84269.5 37 91468.0 38 96000.0 39 99467.0 40 103378.5 41 107969.0 42 109861.0 43 111056.5 44 113296.5 45 118959.0 46 120634.5 47 121964.5 48 124798.0 49 118795.5 50 111717.5 51 105783.0 52 99999.0 53 100019.0 54 102411.0 55 95850.5 56 86000.0 57 76912.5 58 70281.5 59 61041.5 60 46231.0 61 38294.5 62 34134.5 63 24838.0 64 15597.5 65 11125.0 66 7898.5 67 5902.5 68 4606.0 69 3694.5 70 3008.5 71 2099.5 72 1601.5 73 1391.5 74 1036.5 75 744.0 76 505.5 77 318.0 78 208.5 79 138.0 80 79.5 81 33.5 82 16.5 83 8.0 84 2.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0464793087902712 3 2.4838641972088816E-4 4 0.0 5 0.10109327282640149 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.314490739533308E-4 26-27 0.011751782483044522 28-29 0.0 30-31 0.0 32-33 3.5705547834877677E-4 34-35 0.016269310491718175 36-37 0.003166926851441324 38-39 0.012512465893439743 40-41 0.0073429235329987574 42-43 0.005557646141254873 44-45 0.0029961611878832136 46-47 4.6572453697666534E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.707656635581106E-4 68-69 1.707656635581106E-4 70-71 0.0 72-73 1.552415123255551E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.6572453697666534E-5 82-83 0.0010556422838137746 84-85 0.004626197067301542 86-87 0.00152136682079044 88-89 1.0866905862788858E-4 90-91 0.0010245939813486636 92-93 3.415313271162212E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.451592591626645E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3220788.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.8723790880926 #Duplication Level Percentage of deduplicated Percentage of total 1 66.42892622672365 27.15108255153586 2 15.796625684328452 12.912913465651455 3 6.797259195074941 8.334604637433785 4 3.5095374439451987 5.737725793311348 5 2.0372393817341754 4.163341015171531 6 1.2808399924381837 3.141058663327386 7 0.8548106479605564 2.445670139638735 8 0.5960091081851635 1.948824816776 9 0.44822796889596156 1.648812911634137 >10 2.0232736315062194 14.450744522984646 >50 0.13587524521383976 3.8428224569002967 >100 0.07834645564171236 5.958421171608273 >500 0.0073145366189724115 2.006131891613073 >1k 0.00518113010445712 4.722704597483829 >5k 4.5715853862856936E-4 1.2069677224989004 >10k+ 7.619308977142824E-5 0.3281736424308747 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10538 0.32718701137733996 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8742 0.27142426015000054 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6286 0.1951696292956879 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6224 0.193244634542851 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6137 0.19054343222838632 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5861 0.1819741007480157 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5507 0.1709830016753664 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4917 0.1526645032209509 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4871 0.1512362813075558 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4816 0.14952862467197467 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 4195 0.13024762884114074 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3942 0.12239240831746764 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 3938 0.12226821510760721 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3809 0.11826298408960788 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3777 0.11726943841072433 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3621 0.11242590322616702 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3605 0.11192913038672525 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3566 0.1107182465905859 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3440 0.10680616047998191 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3243 0.10068964489435504 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.104830246511102E-5 3.104830246511102E-5 14-15 0.0 0.0 0.0 4.657245369766653E-5 3.104830246511102E-5 16-17 0.0 0.0 0.0 1.2419320986044408E-4 3.104830246511102E-5 18-19 0.0 0.0 0.0 1.552415123255551E-4 3.104830246511102E-5 20-21 0.0 0.0 0.0 2.639105709534437E-4 6.209660493022204E-5 22-23 0.0 0.0 0.0 4.502003857441098E-4 6.209660493022204E-5 24-25 0.0 0.0 0.0 8.383041665579976E-4 6.209660493022204E-5 26-27 0.0 0.0 0.0 0.0016610841818834397 6.209660493022204E-5 28-29 0.0 0.0 0.0 0.004796962730859652 6.209660493022204E-5 30-31 0.0 0.0 0.0 0.015229192359136957 6.209660493022204E-5 32-33 0.0 0.0 0.0 0.039943641121365336 7.762075616277755E-5 34-35 0.0 0.0 0.0 0.07622358255184755 9.314490739533307E-5 36-37 0.0 0.0 0.0 0.1323123409550706 9.314490739533307E-5 38-39 0.0 0.0 0.0 0.22145201733240438 9.314490739533307E-5 40-41 0.0 0.0 0.0 0.3188350180142251 9.314490739533307E-5 42-43 0.0 0.0 0.0 0.41960228366474295 9.314490739533307E-5 44-45 0.0 0.0 0.0 0.5493376155152093 1.0866905862788858E-4 46-47 0.0 0.0 0.0 0.7026541330879276 1.2419320986044408E-4 48-49 0.0 0.0 0.0 0.8584079424041571 1.2419320986044408E-4 50-51 0.0 0.0 0.0 1.0322318637550811 1.2419320986044408E-4 52-53 0.0 0.0 0.0 1.202842285800866 1.2419320986044408E-4 54-55 0.0 0.0 0.0 1.3879677892490907 1.2419320986044408E-4 56-57 0.0 0.0 0.0 1.6044055057333795 1.397173610929996E-4 58-59 0.0 0.0 0.0 1.8283724355654578 1.707656635581106E-4 60-61 0.0 0.0 0.0 2.0777679251164622 1.8628981479066613E-4 62-63 0.0 0.0 0.0 2.3229718938346764 1.8628981479066613E-4 64-65 0.0 0.0 0.0 2.582442557535609 1.8628981479066613E-4 66-67 0.0 0.0 0.0 2.888889302866255 1.8628981479066613E-4 68-69 0.0 0.0 0.0 3.2242730660943844 1.8628981479066613E-4 70-71 0.0 0.0 0.0 3.5723400608795117 1.8628981479066613E-4 72-73 0.0 0.0 0.0 3.935154999335566 1.8628981479066613E-4 74-75 0.0 0.0 0.0 4.314270296585804 1.8628981479066613E-4 76-77 0.0 0.0 0.0 4.723254060807479 1.8628981479066613E-4 78-79 0.0 0.0 0.0 5.186029009049959 1.8628981479066613E-4 80-81 0.0 0.0 0.0 5.660384974111926 1.8628981479066613E-4 82-83 0.0 0.0 0.0 6.162994894417142 2.1733811725577716E-4 84-85 0.0 0.0 0.0 6.677868894196079 2.4838641972088816E-4 86-87 0.0 0.0 0.0 7.207257975377454 2.794347221859992E-4 88-89 0.0 0.0 0.0 7.762277430243779 3.104830246511102E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9465 0.0 35.73198 1 GTATCAA 14865 0.0 34.87945 1 ATCAACG 18885 0.0 27.162487 3 TATCAAC 19050 0.0 27.067142 2 TCAACGC 19095 0.0 26.88866 4 CAACGCA 19230 0.0 26.650452 5 AACGCAG 19690 0.0 26.07434 6 GTGTAAG 1515 0.0 26.059948 1 TAAGGTA 1405 0.0 26.054304 4 TAAGGTG 2650 0.0 23.860022 5 ACGCAGA 21730 0.0 23.49536 7 CGCAGAG 21820 0.0 23.376684 8 GGTAAGG 2530 0.0 23.300552 3 AGGTAAG 2445 0.0 22.956793 2 GTACATG 12005 0.0 22.228428 1 TGTAAGG 1725 0.0 22.060175 2 GTAAGGT 2860 0.0 21.77561 4 TACATGG 11865 0.0 21.648813 2 GAGTACT 12705 0.0 21.625296 12-13 AGAGTAC 18930 0.0 21.614143 10-11 >>END_MODULE