FastQCFastQC Report
Thu 26 May 2016
SRR938516_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938516_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3241873
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106260.32777348156451536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83360.25713530418989267No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA58310.17986515819712864No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA57070.17604020885457264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56550.1744361978399524No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC55540.17132071490770923No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA55410.17091971215405416No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA50260.1550338338361805No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA49110.15148650178461648No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG43390.13384238062379372No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT42940.1324542941688339No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA42580.131343825004866No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA40610.12526709096870853No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA38680.11931374239521414No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG38470.11866596871623286No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT33380.10296516859235386No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT33100.1021014703537122No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG33090.10207062398804642No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95100.036.6252171
GTATCAA150350.035.701541
GTGTAAG13500.030.3950311
ATCAACG187600.028.3973313
TATCAAC191050.027.9591562
TCAACGC190600.027.8755654
CAACGCA192900.027.4446535
AACGCAG198300.026.7886076
TAAGGTA14400.026.7317474
TACATGG126500.025.8465562
GTACATG128350.025.724571
ACATGGG128750.024.2506243
ACGCAGA217900.024.2000547
TGTAAGG15700.023.9128992
CGCAGAG220000.023.8826798
TAAGGTG26950.023.276625
AGGTAAG23350.022.7948062
GCAGAGT232750.021.899489
GAGTACT124100.021.62570612-13
GGTAAGG26650.021.398773
Produced by FastQC (version 0.11.5)