FastQCFastQC Report
Thu 26 May 2016
SRR938516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3241873
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102490.3161444017085185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85670.2642608146586865No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC63020.19439379642570823No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA60680.18717574685991709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60610.18695982230025668No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA58290.1798034654657971No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA56780.1751456642502652No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA49490.1526586636799159No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA49250.15191835090393732No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG49130.15154819451594803No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG42390.1307577440572163No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT40480.12486608821505346No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA39050.12045505792484777No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38450.11860427598490131No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA36650.11305193016506199No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT35630.10990560086715302No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT35550.10965882994182682No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG34200.10549457057694733No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA33980.10481595053230032No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT32980.10173131396572291No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100300.036.475531
GTATCAA149100.032.3444821
TAAGGTG26750.028.941985
TAAGGTA12950.024.940414
ATCAACG193000.024.905833
TCAACGC193950.024.8075624
TATCAAC195150.024.6824022
GTAAGGT28900.024.6523234
CAACGCA195250.024.6180635
GGTAAGG27300.024.5320763
AGGTAAG27050.024.4098742
AACGCAG201750.023.8013746
AAGGTAA27650.023.5416661
GTACATG122900.023.0412581
GTGTAAG14300.022.925821
TACATGG121400.022.8122392
ACGCAGA224250.021.3709167
ACATGGG123150.021.3674723
GAGTACT128800.021.31456212-13
CGCAGAG224800.021.2552438
Produced by FastQC (version 0.11.5)