##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938516_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3241873 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.856416337098956 31.0 31.0 34.0 30.0 34.0 2 32.111080847399016 33.0 31.0 34.0 30.0 34.0 3 32.162368482664185 34.0 31.0 34.0 30.0 34.0 4 35.816286757686065 37.0 35.0 37.0 35.0 37.0 5 35.71254333528797 37.0 35.0 37.0 33.0 37.0 6 35.74333541135017 37.0 35.0 37.0 33.0 37.0 7 35.66811901638343 37.0 35.0 37.0 33.0 37.0 8 35.632419283543804 37.0 35.0 37.0 33.0 37.0 9 37.19162687742549 39.0 37.0 39.0 33.0 39.0 10-11 37.12524642390371 39.0 37.0 39.0 33.0 39.0 12-13 37.02259619670481 39.0 37.0 39.0 33.0 39.0 14-15 38.04548481695612 40.0 37.0 41.0 33.0 41.0 16-17 38.13066258301914 40.0 37.0 41.0 33.0 41.0 18-19 38.2839525175724 40.0 38.0 41.0 33.5 41.0 20-21 38.24675288020228 40.0 38.0 41.0 33.0 41.0 22-23 38.135628847891326 40.0 37.0 41.0 33.0 41.0 24-25 38.02053180368262 40.0 37.0 41.0 33.0 41.0 26-27 37.81493368185613 40.0 37.0 41.0 32.0 41.0 28-29 37.68899414011591 40.0 37.0 41.0 32.0 41.0 30-31 37.41635514407875 39.0 36.5 41.0 32.0 41.0 32-33 37.28655178657523 39.0 36.0 41.0 31.0 41.0 34-35 37.15313616542042 39.0 36.0 41.0 31.0 41.0 36-37 37.01233330238415 39.0 36.0 41.0 31.0 41.0 38-39 36.79486210594925 39.0 35.0 41.0 30.0 41.0 40-41 36.57874028378039 39.0 35.0 40.5 30.0 41.0 42-43 36.32076750076267 39.0 35.0 40.0 30.0 41.0 44-45 36.035879567151454 38.5 35.0 40.0 29.5 41.0 46-47 35.63060921880654 38.0 35.0 40.0 28.0 41.0 48-49 35.2237283817102 38.0 34.0 40.0 27.0 41.0 50-51 34.83713473661676 38.0 34.0 40.0 26.0 41.0 52-53 34.28235236235349 38.0 33.0 40.0 24.0 41.0 54-55 33.90052648576918 37.0 33.0 40.0 23.0 41.0 56-57 33.53795599025625 37.0 32.5 40.0 23.0 41.0 58-59 33.04371176785766 36.5 31.5 39.0 21.0 40.5 60-61 32.606067696051014 36.0 31.0 39.0 19.5 40.0 62-63 32.176252277618524 35.5 31.0 39.0 18.0 40.0 64-65 31.596125449701454 35.0 30.0 38.0 14.0 40.0 66-67 31.00509551114433 35.0 29.0 38.0 9.5 40.0 68-69 30.351299541962316 34.0 28.5 37.5 7.5 39.5 70-71 29.628279855503283 34.0 28.0 36.5 6.0 39.0 72-73 29.10413054428721 34.0 27.0 36.0 2.0 39.0 74-75 28.455346029903083 33.0 26.0 35.5 2.0 38.5 76-77 27.271407454887964 31.5 25.5 34.5 2.0 36.5 78-79 27.819570816006674 32.5 26.0 35.0 2.0 37.0 80-81 27.499742124383033 33.0 26.0 35.0 2.0 36.5 82-83 26.879870371232926 32.0 25.0 35.0 2.0 36.0 84-85 26.163256549531706 32.0 23.5 34.5 2.0 36.0 86-87 25.364780020685572 31.0 21.0 34.0 2.0 35.0 88-89 24.463109288982018 31.0 18.0 34.0 2.0 35.0 90-91 23.69084384243306 30.0 10.0 34.0 2.0 35.0 92-93 22.72261112634579 29.5 2.0 34.0 2.0 35.0 94-95 21.648560261305732 29.0 2.0 34.0 2.0 35.0 96-97 20.35350891290313 28.0 2.0 33.5 2.0 35.0 98-99 18.623609407277833 25.5 2.0 33.0 2.0 34.0 100-101 14.09638872343241 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2914647229901739 1101 2 0.23350639469678214 1101 3 0.2922917381895118 1101 4 0.1574440120017968 1101 5 0.18494601365839713 1101 6 0.26115918675372285 1101 7 0.26483970405055146 1101 8 0.31752368601071623 1101 9 0.22299030699985423 1101 10-11 0.3524846180125891 1101 12-13 0.4557512723164905 1101 14-15 0.4779163382551843 1101 16-17 0.4475485090399971 1101 18-19 0.43410060103639125 1101 20-21 0.5111347665086114 1101 22-23 0.4232565433231841 1101 24-25 0.37756265690217816 1101 26-27 0.5021713677796669 1101 28-29 0.42781215376260917 1101 30-31 0.4256913665334281 1101 32-33 0.43933266675775684 1101 34-35 0.3384558760526204 1101 36-37 0.37972378695526743 1101 38-39 0.5554728186610731 1101 40-41 0.5122406410265512 1101 42-43 0.43426980136529636 1101 44-45 0.49672120923572294 1101 46-47 0.5935370420353294 1101 48-49 0.5589272211710394 1101 50-51 0.6892499967015695 1101 52-53 0.7855345205536182 1101 54-55 0.7614600860029412 1101 56-57 0.7746301505061268 1101 58-59 0.8369540223268288 1101 60-61 0.7436343552879165 1101 62-63 0.8496202817796785 1101 64-65 0.7706059738401372 1101 66-67 1.000342651789552 1101 68-69 1.0092265984830178 1101 70-71 1.2065534301646963 1101 72-73 1.3834017828719496 1101 74-75 1.3180036307685015 1101 76-77 1.3870117523598218 1101 78-79 1.392160828945343 1101 80-81 1.4853588754493714 1101 82-83 1.6086671627015718 1101 84-85 1.8620135354919825 1101 86-87 1.8176947581223644 1101 88-89 1.6279747590388887 1101 90-91 1.8322053129356703 1101 92-93 1.973005037363258 1101 94-95 2.0212363219026983 1101 96-97 2.2507703467281317 1101 98-99 2.174905742632834 1101 100-101 1.9844487804278188 1102 1 0.2538118759186041 1102 2 0.23821689957494385 1102 3 0.1692961461324387 1102 4 0.21794730457244071 1102 5 0.18057472127697594 1102 6 0.2846048787030284 1102 7 0.1925219391411872 1102 8 0.22063913834863058 1102 9 0.31506661499544464 1102 10-11 0.34665068293448087 1102 12-13 0.3419005112588067 1102 14-15 0.41466061570137214 1102 16-17 0.26498018436502946 1102 18-19 0.34678691447497556 1102 20-21 0.2239061300076841 1102 22-23 0.3212352007487169 1102 24-25 0.20612063208690046 1102 26-27 0.3039579389157794 1102 28-29 0.30249562041554867 1102 30-31 0.24541383092651614 1102 32-33 0.28976036042990216 1102 34-35 0.3486495364959694 1102 36-37 0.38875060839641407 1102 38-39 0.44257094282896503 1102 40-41 0.4909130127015544 1102 42-43 0.39003441232625846 1102 44-45 0.3577033502826197 1102 46-47 0.2854081069754173 1102 48-49 0.4370836519381598 1102 50-51 0.4962726571360818 1102 52-53 0.37543518977698653 1102 54-55 0.2657965473693764 1102 56-57 0.5094894462022097 1102 58-59 0.6101905982197238 1102 60-61 0.6152330676840663 1102 62-63 0.7904178236457824 1102 64-65 0.8893718070058192 1102 66-67 1.0685198148085817 1102 68-69 1.0926346625388135 1102 70-71 1.2717707367083015 1102 72-73 1.4176921373321143 1102 74-75 1.3636420078927394 1102 76-77 1.38479283475181 1102 78-79 1.529941147134462 1102 80-81 1.6250660765797704 1102 82-83 1.6340828864614743 1102 84-85 1.602884300469448 1102 86-87 1.5870533943685565 1102 88-89 1.4502242915494854 1102 90-91 1.7212429267927973 1102 92-93 2.0157872712582012 1102 94-95 1.801479910266643 1102 96-97 1.8444043029127961 1102 98-99 1.933223986858554 1102 100-101 1.8528637041141067 1103 1 0.27660644966392667 1103 2 0.2730392784448412 1103 3 0.26860082326327017 1103 4 0.19044575383836104 1103 5 0.1964679197621777 1103 6 0.15258652891779434 1103 7 0.27570506793342986 1103 8 0.22546369847086112 1103 9 0.26526760181737075 1103 10-11 0.31065259445159654 1103 12-13 0.3936116501549165 1103 14-15 0.5327356264372227 1103 16-17 0.46211987118004316 1103 18-19 0.326321606611657 1103 20-21 0.3533285217313846 1103 22-23 0.48868806768356166 1103 24-25 0.4026930959185222 1103 26-27 0.5337484900033331 1103 28-29 0.4745289955237624 1103 30-31 0.39236694276536355 1103 32-33 0.4829894951446292 1103 34-35 0.5758587361224272 1103 36-37 0.642888414855058 1103 38-39 0.5756851528115021 1103 40-41 0.6765947952521216 1103 42-43 0.6264701145580531 1103 44-45 0.7642116132364833 1103 46-47 0.7789896015811948 1103 48-49 0.739073290015817 1103 50-51 0.7190785753410651 1103 52-53 0.6661846836582797 1103 54-55 0.9156091446858454 1103 56-57 1.0481032116572138 1103 58-59 1.0783619638297424 1103 60-61 0.9123992411345299 1103 62-63 1.0652601210498034 1103 64-65 1.416766729556759 1103 66-67 1.156994793215155 1103 68-69 1.2611995786531125 1103 70-71 1.5414323163829238 1103 72-73 1.5192377104123445 1103 74-75 1.519270710057338 1103 76-77 1.6002533370435863 1103 78-79 1.6039692239418102 1103 80-81 1.8585529496279634 1103 82-83 1.8511269876832657 1103 84-85 1.967092335462965 1103 86-87 1.8305222964293506 1103 88-89 1.979571873134649 1103 90-91 2.363744677150418 1103 92-93 2.556869673907645 1103 94-95 2.2834361333113797 1103 96-97 2.1787351453338673 1103 98-99 2.4072449307760273 1103 100-101 2.1574385887463094 1104 1 0.15265390767705966 1104 2 0.17096269895818494 1104 3 0.17883348660306098 1104 4 0.20634709787533723 1104 5 0.08182430991173106 1104 6 0.16456961446873208 1104 7 0.17859134686671752 1104 8 0.19301228245512903 1104 9 0.21720625376340053 1104 10-11 0.15651397245375165 1104 12-13 0.23567111030747157 1104 14-15 0.322248571630702 1104 16-17 0.32553977479888374 1104 18-19 0.2919510184211944 1104 20-21 0.287498271013952 1104 22-23 0.3278587382763689 1104 24-25 0.30077914674346573 1104 26-27 0.4327143809013734 1104 28-29 0.3165223836096729 1104 30-31 0.27580472114787824 1104 32-33 0.5594639053603672 1104 34-35 0.38371550622839834 1104 36-37 0.40490198658432774 1104 38-39 0.3492485589066803 1104 40-41 0.3646875664860829 1104 42-43 0.34896614575078644 1104 44-45 0.3737467707491504 1104 46-47 0.45543289796199105 1104 48-49 0.4503789883757108 1104 50-51 0.46301305875053345 1104 52-53 0.5804608005620153 1104 54-55 0.6334483560980786 1104 56-57 0.79987835905564 1104 58-59 0.7534652133425226 1104 60-61 0.593991843573697 1104 62-63 0.701919290955999 1104 64-65 0.8547376681589363 1104 66-67 0.6974878914582128 1104 68-69 0.7486560293715314 1104 70-71 0.7813794430382011 1104 72-73 1.0217299554907342 1104 74-75 1.0273863100589544 1104 76-77 1.1134209002878315 1104 78-79 1.1592900371695833 1104 80-81 1.3191195823264508 1104 82-83 1.3483437789322998 1104 84-85 1.3754765500784885 1104 86-87 1.3884463714988797 1104 88-89 1.3518910508686197 1104 90-91 1.5169517334453886 1104 92-93 1.6129492791475002 1104 94-95 1.7786950842550837 1104 96-97 2.102444660921943 1104 98-99 2.255623155754158 1104 100-101 2.0371498801542707 1105 1 0.13678411256918466 1105 2 0.17653448261896898 1105 3 0.17623065326095855 1105 4 0.08733418957377381 1105 5 0.1291766991820893 1105 6 0.06175796001804201 1105 7 0.14025650484997243 1105 8 0.08309117404700572 1105 9 0.17665055414862252 1105 10-11 0.23907104012353386 1105 12-13 0.24866054364219536 1105 14-15 0.3369202946185723 1105 16-17 0.22596288881596394 1105 18-19 0.30760777041748355 1105 20-21 0.25032285193551473 1105 22-23 0.29869120909229707 1105 24-25 0.24083337322577592 1105 26-27 0.32187692980230054 1105 28-29 0.34754664888925646 1105 30-31 0.31988553070540604 1105 32-33 0.38760223204194943 1105 34-35 0.3094529415422187 1105 36-37 0.3886539441181185 1105 38-39 0.39380287345241527 1105 40-41 0.47831500538860894 1105 42-43 0.46556736611970706 1105 44-45 0.542381394933642 1105 46-47 0.5490083432126411 1105 48-49 0.5777695269089946 1105 50-51 0.6404740322667024 1105 52-53 0.6935342328519525 1105 54-55 0.6233325629396802 1105 56-57 0.69752319555122 1105 58-59 0.8773891408303527 1105 60-61 0.9793189863163576 1105 62-63 1.011284407779982 1105 64-65 1.2056089078849972 1105 66-67 1.2175441386205677 1105 68-69 1.1715428249731303 1105 70-71 1.2122575345920197 1105 72-73 1.064628341645836 1105 74-75 1.1069655002892809 1105 76-77 1.1800893733836446 1105 78-79 1.189061669867506 1105 80-81 1.497760248596336 1105 82-83 1.5692392909746182 1105 84-85 1.671145363140063 1105 86-87 1.724537029876135 1105 88-89 1.6939944926727755 1105 90-91 1.6372459461400268 1105 92-93 1.7336310864210454 1105 94-95 1.614054033803935 1105 96-97 1.6193173392797782 1105 98-99 1.832386206968419 1105 100-101 2.0024050534995297 1106 1 0.00804034042979751 1106 2 0.08156146104719397 1106 3 0.029206747291347313 1106 4 0.058589584954852114 1106 5 0.1459085226005925 1106 6 -0.0175476951981679 1106 7 0.001174453636643591 1106 8 0.013197004530617562 1106 9 -0.028115147532133733 1106 10-11 0.06849836933020015 1106 12-13 0.07561789591870394 1106 14-15 0.10108127910480391 1106 16-17 -0.008476241295710452 1106 18-19 0.10385982572823593 1106 20-21 0.08765452355847714 1106 22-23 0.17439531419100973 1106 24-25 0.07914199003869982 1106 26-27 -0.13624077102729615 1106 28-29 0.03981612210838392 1106 30-31 0.2043641223774415 1106 32-33 -0.02932001599391043 1106 34-35 -0.013484245237350478 1106 36-37 0.11439005907013922 1106 38-39 0.10885634706463776 1106 40-41 0.08590120864437267 1106 42-43 0.25765585625766363 1106 44-45 0.3039555507892402 1106 46-47 -0.008498632356719327 1106 48-49 0.0764151740765513 1106 50-51 0.2743695329381737 1106 52-53 0.2349949115948604 1106 54-55 0.2171837168172317 1106 56-57 0.3202058567893076 1106 58-59 0.42862658104919404 1106 60-61 0.6175978470620223 1106 62-63 0.5123262762214154 1106 64-65 0.7078411205384505 1106 66-67 0.5420473006214905 1106 68-69 0.39142605042929546 1106 70-71 0.2602838880514575 1106 72-73 0.5427364648999173 1106 74-75 0.6097322257168223 1106 76-77 0.6237471077479526 1106 78-79 0.8346997512174035 1106 80-81 0.8866042817282036 1106 82-83 1.0967115724517846 1106 84-85 1.0146716469554846 1106 86-87 1.0887208364842351 1106 88-89 1.0460245549300602 1106 90-91 1.1259321939435978 1106 92-93 1.189826825506529 1106 94-95 1.1091577851191516 1106 96-97 1.3731533948211911 1106 98-99 1.7675876243764996 1106 100-101 1.907552132328842 1107 1 0.020837689711552088 1107 2 0.04151356797436989 1107 3 0.058554106949998186 1107 4 0.08181229969493842 1107 5 0.029725042390872147 1107 6 -0.009982615747574641 1107 7 -7.701234963164438E-4 1107 8 0.09341780665896238 1107 9 0.09930563805388459 1107 10-11 0.08054282455835704 1107 12-13 0.07116190135490541 1107 14-15 0.18262964094014222 1107 16-17 0.07666201423999297 1107 18-19 0.040431762277030714 1107 20-21 0.15754452846427114 1107 22-23 0.22607149585763864 1107 24-25 0.24822781398508198 1107 26-27 0.3278410842781838 1107 28-29 0.08991509151380939 1107 30-31 0.03972324264432814 1107 32-33 0.05735955002041493 1107 34-35 0.08513809380978898 1107 36-37 -0.012977276966374518 1107 38-39 -0.007582713998935731 1107 40-41 -0.1378563430608324 1107 42-43 -0.029964611533550567 1107 44-45 -0.04373547609849027 1107 46-47 0.03462021477965749 1107 48-49 0.08179318522592638 1107 50-51 -0.04031570944676588 1107 52-53 0.10954227406114825 1107 54-55 0.2597213534574223 1107 56-57 0.1235278767137089 1107 58-59 0.23606654017901718 1107 60-61 0.27867634040506317 1107 62-63 0.4299570108790647 1107 64-65 0.5130288949238668 1107 66-67 0.4837494453949347 1107 68-69 0.716376736461612 1107 70-71 0.7678299969432238 1107 72-73 0.8720187934828942 1107 74-75 0.7092524887398248 1107 76-77 0.709903465749715 1107 78-79 0.8871350872220454 1107 80-81 1.018258489796537 1107 82-83 1.3188928129973299 1107 84-85 1.2467855875465261 1107 86-87 1.457224510891777 1107 88-89 1.3408087799998079 1107 90-91 1.5688366453093465 1107 92-93 1.6662963134494113 1107 94-95 1.198950609543214 1107 96-97 1.198857164612697 1107 98-99 1.4090484257004938 1107 100-101 1.5267432049356202 1108 1 0.013891619042532 1108 2 0.08246517704439071 1108 3 0.06625354640671333 1108 4 0.0546674315954121 1108 5 0.009446851346503138 1108 6 -0.0023806772201666604 1108 7 0.06830745677437022 1108 8 0.03579823961532469 1108 9 -0.0922819469438565 1108 10-11 -0.031694543195129654 1108 12-13 0.017929919657227344 1108 14-15 0.08109265196035409 1108 16-17 0.011518683521195783 1108 18-19 -0.10288720417829467 1108 20-21 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0.50738480647016 2316 98-99 0.6709141412125881 2316 100-101 0.2637407454267624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 12.0 4 46.0 5 87.0 6 236.0 7 627.0 8 1421.0 9 2830.0 10 4751.0 11 7254.0 12 10427.0 13 14295.0 14 18939.0 15 24942.0 16 31061.0 17 38596.0 18 37496.0 19 33901.0 20 33887.0 21 35476.0 22 39401.0 23 44542.0 24 51190.0 25 58443.0 26 68529.0 27 80909.0 28 96080.0 29 113675.0 30 134268.0 31 159152.0 32 189981.0 33 231600.0 34 287367.0 35 362149.0 36 438322.0 37 423370.0 38 162320.0 39 4286.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.63561455414083 19.801749861233542 15.796050485998858 22.766585098626777 2 15.37823885987073 21.054482598411763 39.62409496717063 23.94318357454688 3 18.529120715056006 24.87497748175714 31.04491433196702 25.550987471219834 4 12.832458273350007 17.700076468140484 37.64330064749606 31.82416461101345 5 12.999280660755662 38.18300212470356 34.779929077839164 14.0377881367016 6 31.147703811963023 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21.896635362511983 28-29 21.331083392481425 27.56743576147419 29.792051669055724 21.309429176988665 30-31 21.453462242351875 26.907037999329397 29.524059085596505 22.11544067272222 32-33 20.88169096674858 27.137294421738865 29.554902330034764 22.426112281477785 34-35 22.056511826243323 27.201369983666506 29.218708569508824 21.523409620581347 36-37 22.335690387171812 26.394509643397345 29.34944604582073 21.920353923610108 38-39 21.506606059446824 26.89628428052167 29.35590226252831 22.24120739750319 40-41 22.054306427746067 26.850632257674405 29.434206623322755 21.66085469125677 42-43 21.844707589526372 26.8513021547902 29.000863594220842 22.30312666146258 44-45 21.65829745610753 27.071869303973077 29.45896824738274 21.81086499253665 46-47 22.419820643779172 27.40690758196073 28.405682209895673 21.767589564364425 48-49 21.946032432485787 27.589112836930994 28.131715832174798 22.333138898408418 50-51 22.1529498533718 27.60888535732276 28.380029075784275 21.858135713521165 52-53 22.592726488668742 27.163170796635157 28.40814553808863 21.835957176607472 54-55 21.673890371399494 27.54913594702815 28.607628984849192 22.169344696723158 56-57 22.040715351896882 27.638112288791078 28.188010449514834 22.133161909797206 58-59 21.89539812324542 27.07499646037954 28.269861280808968 22.759744135566077 60-61 21.254148450602475 27.593107441284715 28.75510545909726 22.39763864901555 62-63 21.57561385038834 27.621840830902382 28.781648756752652 22.020896561956622 64-65 21.17924222747982 27.583262129573367 28.4945263073473 22.742969335599515 66-67 21.10706523943655 27.641219112355735 28.377958823209156 22.873756824998562 68-69 21.924781675115053 27.144729093913554 28.489041572957113 22.441447658014287 70-71 21.74324306191655 27.194584979694596 27.979440270843554 23.082731687545298 72-73 21.494181749534526 27.441771225725414 28.144020669564583 22.920026355175473 74-75 21.60618259876312 27.899982510110664 27.81666647644741 22.677168414678796 76-77 21.605118399147653 27.247165450343058 28.37208613662534 22.775630013883948 78-79 21.604100469080684 27.555875877926123 28.17639679284167 22.663626860151524 80-81 22.05071115579725 27.554061767735632 27.757940978826884 22.637286097640235 82-83 21.9323868648072 27.55302119274529 27.95820769097061 22.5563842514769 84-85 21.56529496714464 27.811646235310793 27.992183751253393 22.630875046291177 86-87 21.911798048285426 27.814940318005775 27.458868856934416 22.81439277677438 88-89 21.54287043323412 27.869043605347898 27.21122943434243 23.376856527075553 90-91 21.466222062504723 27.65470790462809 27.62672992302199 23.252340109845196 92-93 21.67780429888749 27.299403861114385 27.781780964149615 23.24101087584851 94-95 21.18950754055297 26.97127677196286 28.037913908357243 23.80130177912693 96-97 21.45158061404626 26.53211584784475 28.170057864697352 23.846245673411637 98-99 21.120583687269672 27.02015162222579 27.58501027029745 24.274254420207082 100-101 21.230862718999592 26.576569559722042 28.028353983693066 24.1642137375853 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 403.0 1 311.5 2 283.5 3 350.0 4 338.5 5 378.0 6 657.5 7 1514.0 8 2425.0 9 2755.5 10 2476.0 11 2081.5 12 2085.0 13 2260.0 14 2521.5 15 2846.5 16 3152.0 17 3522.5 18 3964.5 19 4508.0 20 5181.5 21 6186.0 22 7560.5 23 9107.0 24 11318.0 25 14509.0 26 18680.5 27 23803.0 28 29285.0 29 35811.0 30 42860.0 31 52823.0 32 68159.0 33 74325.0 34 72361.0 35 76677.0 36 85165.5 37 92226.0 38 96859.5 39 100463.0 40 104231.5 41 108431.0 42 110280.0 43 111621.0 44 113465.0 45 118826.0 46 121068.0 47 122697.5 48 125166.0 49 119404.0 50 112312.5 51 106306.0 52 101050.0 53 100862.0 54 102716.0 55 96306.5 56 86449.5 57 77807.5 58 70995.5 59 61363.5 60 47163.0 61 38807.5 62 34411.0 63 25607.0 64 16388.5 65 11588.5 66 8264.0 67 6119.0 68 4786.0 69 3839.5 70 3088.0 71 2198.5 72 1628.5 73 1382.5 74 1075.5 75 775.0 76 563.0 77 355.5 78 203.5 79 142.0 80 73.5 81 29.0 82 17.5 83 8.0 84 3.5 85 1.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025170634383271644 2 0.012832088116962015 3 0.0017582428429491223 4 0.0 5 2.776172909919667E-4 6 0.0 7 0.0 8 6.169273133154815E-5 9 3.701563879892889E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016811269287846872 26-27 0.008960869225907369 28-29 3.0846365665774075E-4 30-31 0.0 32-33 3.547332051564019E-4 34-35 0.011551963941832392 36-37 0.0013418169064611722 38-39 0.014035096377927204 40-41 0.013371899516113062 42-43 0.005814539927998413 44-45 9.253909699732223E-4 46-47 1.542318283288704E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 9.253909699732222E-5 66-67 6.940432274799167E-4 68-69 9.253909699732223E-4 70-71 4.626954849866111E-5 72-73 1.5423182832887037E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.711591416443519E-5 82-83 0.0010025068841376574 84-85 0.005074227152019836 86-87 0.0033314074919036 88-89 0.0 90-91 0.0011721618952994148 92-93 3.3931002232351484E-4 94-95 6.169273133154815E-5 96-97 0.0 98-99 0.0 100-101 1.542318283288704E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3241873.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.811775410139475 #Duplication Level Percentage of deduplicated Percentage of total 1 66.36059363693386 27.08293643594076 2 15.892101428235433 12.971697485686025 3 6.787714530029381 8.310560428430986 4 3.5008063861831 5.714964959491467 5 2.0000872995371144 4.081355683469053 6 1.251255608906663 3.0639577734825636 7 0.8939534880570147 2.553868028718558 8 0.6291548010943236 2.0541539552378008 9 0.43849842790863997 1.6106309421755987 >10 2.0128241560229303 14.343828476895068 >50 0.14170765145465303 3.9949209305966633 >100 0.07840534727473045 5.978179225352181 >500 0.007131414153706089 1.9597591339564642 >1k 0.005234761452567166 4.769650292723988 >5k 4.5519664804931874E-4 1.192203577618111 >10k+ 7.58661080082198E-5 0.3173326702248544 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10249 0.3161444017085185 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8567 0.2642608146586865 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 6302 0.19439379642570823 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 6068 0.18717574685991709 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6061 0.18695982230025668 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 5829 0.1798034654657971 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5678 0.1751456642502652 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 4949 0.1526586636799159 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4925 0.15191835090393732 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 4913 0.15154819451594803 No Hit CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG 4239 0.1307577440572163 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 4048 0.12486608821505346 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 3905 0.12045505792484777 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3845 0.11860427598490131 No Hit GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA 3665 0.11305193016506199 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 3563 0.10990560086715302 No Hit GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT 3555 0.10965882994182682 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 3420 0.10549457057694733 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 3398 0.10481595053230032 No Hit GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT 3298 0.10173131396572291 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.084636566577408E-5 0.0 3 0.0 0.0 0.0 3.084636566577408E-5 0.0 4 0.0 0.0 0.0 6.169273133154815E-5 0.0 5 0.0 0.0 0.0 6.169273133154815E-5 0.0 6 0.0 0.0 0.0 6.169273133154815E-5 0.0 7 0.0 0.0 0.0 6.169273133154815E-5 0.0 8 0.0 0.0 0.0 6.169273133154815E-5 0.0 9 0.0 0.0 0.0 9.253909699732223E-5 0.0 10-11 0.0 0.0 0.0 1.0796227983020926E-4 0.0 12-13 0.0 0.0 0.0 1.5423182832887037E-4 3.084636566577408E-5 14-15 0.0 0.0 0.0 1.6965501116175742E-4 3.084636566577408E-5 16-17 0.0 0.0 0.0 3.0846365665774075E-4 3.084636566577408E-5 18-19 0.0 0.0 0.0 4.0100275365506297E-4 3.084636566577408E-5 20-21 0.0 0.0 0.0 4.6269548498661115E-4 1.233854626630963E-4 22-23 0.0 0.0 0.0 6.631968618141426E-4 1.233854626630963E-4 24-25 0.0 0.0 0.0 0.001187585078132302 1.233854626630963E-4 26-27 0.0 0.0 0.0 0.0022517846936015076 1.542318283288704E-4 28-29 0.0 0.0 0.0 0.005135919883351384 1.8507819399464446E-4 30-31 0.0 0.0 0.0 0.016225188340197164 1.8507819399464446E-4 32-33 0.0 0.0 0.0 0.041149051798142613 2.1592455966041853E-4 34-35 0.0 0.0 0.0 0.07921346702970783 2.1592455966041853E-4 36-37 0.0 0.0 0.0 0.13651059125388318 2.1592455966041853E-4 38-39 0.0 0.0 0.0 0.2245306956811695 2.1592455966041853E-4 40-41 0.0 0.0 0.0 0.31873549642444354 2.1592455966041853E-4 42-43 0.0 0.0 0.0 0.4207290044983255 2.1592455966041853E-4 44-45 0.0 0.0 0.0 0.5522733308800191 2.467709253261926E-4 46-47 0.0 0.0 0.0 0.7055026523247518 2.9304047382485376E-4 48-49 0.0 0.0 0.0 0.8578220059823441 3.3931002232351484E-4 50-51 0.0 0.0 0.0 1.0316258533261482 3.7015638798928893E-4 52-53 0.0 0.0 0.0 1.2026689509428654 3.7015638798928893E-4 54-55 0.0 0.0 0.0 1.3883332258851597 3.7015638798928893E-4 56-57 0.0 0.0 0.0 1.6037179741464271 3.7015638798928893E-4 58-59 0.0 0.0 0.0 1.8247013377760326 3.7015638798928893E-4 60-61 0.0 0.0 0.0 2.0726444249975247 3.7015638798928893E-4 62-63 0.0 0.0 0.0 2.314526201365692 4.3184911932083706E-4 64-65 0.0 0.0 0.0 2.56939429767915 4.3184911932083706E-4 66-67 0.0 0.0 0.0 2.871195139353084 4.3184911932083706E-4 68-69 0.0 0.0 0.0 3.207374255561523 4.3184911932083706E-4 70-71 0.0 0.0 0.0 3.554534677946977 4.3184911932083706E-4 72-73 0.0 0.0 0.0 3.92017207336623 4.3184911932083706E-4 74-75 0.0 0.0 0.0 4.2980708991376275 4.472723021537241E-4 76-77 0.0 0.0 0.0 4.706291702358483 4.6269548498661115E-4 78-79 0.0 0.0 0.0 5.16678167219999 4.6269548498661115E-4 80-81 0.0 0.0 0.0 5.636232511267407 4.6269548498661115E-4 82-83 0.0 0.0 0.0 6.1301753646734465 4.6269548498661115E-4 84-85 0.0 0.0 0.0 6.6440141239339106 4.935418506523852E-4 86-87 0.0 0.0 0.0 7.168063030229747 5.243882163181593E-4 88-89 0.0 0.0 0.0 7.7181616923303284 5.552345819839333E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10030 0.0 36.47553 1 GTATCAA 14910 0.0 32.344482 1 TAAGGTG 2675 0.0 28.94198 5 TAAGGTA 1295 0.0 24.94041 4 ATCAACG 19300 0.0 24.90583 3 TCAACGC 19395 0.0 24.807562 4 TATCAAC 19515 0.0 24.682402 2 GTAAGGT 2890 0.0 24.652323 4 CAACGCA 19525 0.0 24.618063 5 GGTAAGG 2730 0.0 24.532076 3 AGGTAAG 2705 0.0 24.409874 2 AACGCAG 20175 0.0 23.801374 6 AAGGTAA 2765 0.0 23.541666 1 GTACATG 12290 0.0 23.041258 1 GTGTAAG 1430 0.0 22.92582 1 TACATGG 12140 0.0 22.812239 2 ACGCAGA 22425 0.0 21.370916 7 ACATGGG 12315 0.0 21.367472 3 GAGTACT 12880 0.0 21.314562 12-13 CGCAGAG 22480 0.0 21.255243 8 >>END_MODULE