FastQCFastQC Report
Thu 26 May 2016
SRR938515_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938515_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102549
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114000.5421990165270821No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88210.41953837936713967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63900.3039168171586013No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33360.15866455431003035No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32150.152909634924085No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31080.1478205739794887No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA28860.13726196155238238No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28270.1344558438352685No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26300.12508626433914263No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24290.11552643957405988No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24190.1150508264016677No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA22760.10824955803645955No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA21670.10306537445738481No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21210.10087755386438081No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73200.038.463871
GTATCAA115050.035.2618261
TATCAAC142700.028.294722
ATCAACG145000.027.8131083
TCAACGC147200.027.3974234
GTACATG102100.027.2503761
CAACGCA148550.027.0523955
TAAGGTG13050.026.9676385
TAAGGTA7850.026.6565974
TACATGG102300.026.5914522
AACGCAG152300.026.4591756
GTGTAAG9250.025.7081411
AGGTAAG10400.025.1507552
GTGCGAA1151.2783031E-424.7938569
ACATGGG106150.024.6861443
GTAAGGT13350.024.5804334
ACGCAGA169250.023.6414497
CGCAGAG170450.023.363498
GAGTACT94950.022.72009712-13
GGTAAGG13650.021.949753
Produced by FastQC (version 0.11.5)