FastQCFastQC Report
Thu 26 May 2016
SRR938515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938515_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102549
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108560.5163256599489476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91920.4371836280628894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69260.32940968319882197No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33380.1587596769445088No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA33170.15776088928248522No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31760.1510547435517555No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30060.1429693196210885No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA28790.13692903233170783No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28080.13355217880772338No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27600.13126923558024092No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA23670.11257763790522837No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21830.10382635553321232No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21460.10206658679536125No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA21310.101353167036773No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA21200.10082999254714159No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79400.037.115771
GTATCAA121450.033.5014421
AGGTAAG13150.028.5554242
TAAGGTG14350.028.1401795
TATCAAC150050.026.9892522
ATCAACG152750.026.4050083
TCAACGC154000.026.1906834
CAACGCA156750.025.7918155
GGTAAGG15300.025.1508963
AACGCAG160750.025.0777916
GTAAGGT15400.024.9875764
GTGTAAG8600.024.8714621
TAAGGTA7800.023.7536244
TACATGG103600.023.628422
GTACATG106250.023.4417251
AAGGTAA15950.022.9465451
ACATGGG105450.022.5259593
GAGTACT99700.022.36006412-13
ACGCAGA180300.022.332267
CGCAGAG181050.022.0561688
Produced by FastQC (version 0.11.5)