##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938515_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2102549 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78755263254269 31.0 31.0 34.0 30.0 34.0 2 32.000955506863335 31.0 31.0 34.0 30.0 34.0 3 32.05357639703046 33.0 31.0 34.0 30.0 34.0 4 35.74261479756239 37.0 35.0 37.0 33.0 37.0 5 35.64396644263701 37.0 35.0 37.0 33.0 37.0 6 35.650689234828775 37.0 35.0 37.0 33.0 37.0 7 35.58840436061181 37.0 35.0 37.0 33.0 37.0 8 35.56013581609751 37.0 35.0 37.0 33.0 37.0 9 37.13293007677824 39.0 37.0 39.0 33.0 39.0 10-11 37.036940399486525 39.0 37.0 39.0 33.0 39.0 12-13 36.926064743318705 39.0 37.0 39.0 33.0 39.0 14-15 37.930775929597836 40.0 37.0 41.0 33.0 41.0 16-17 37.848563576877396 40.0 37.0 41.0 32.5 41.0 18-19 38.10847357184066 40.0 37.0 41.0 33.0 41.0 20-21 38.08208583961658 40.0 37.0 41.0 33.0 41.0 22-23 37.97839455822432 40.0 37.0 41.0 33.0 41.0 24-25 37.841756363347535 40.0 37.0 41.0 32.0 41.0 26-27 37.635140251190336 40.0 37.0 41.0 32.0 41.0 28-29 37.512904812206514 39.5 36.5 41.0 32.0 41.0 30-31 37.223201219091685 39.0 36.0 41.0 31.0 41.0 32-33 36.98228198248887 39.0 35.5 40.5 31.0 41.0 34-35 36.85978115135485 39.0 35.0 41.0 30.5 41.0 36-37 36.781311874301146 39.0 35.0 41.0 30.0 41.0 38-39 36.5987220749671 39.0 35.0 41.0 30.0 41.0 40-41 36.400741671181024 39.0 35.0 40.0 30.0 41.0 42-43 36.15331723541283 39.0 35.0 40.0 30.0 41.0 44-45 35.80993593966181 38.0 35.0 40.0 28.5 41.0 46-47 35.36507543938334 38.0 34.5 40.0 27.0 41.0 48-49 34.94298896244511 38.0 34.0 40.0 26.0 41.0 50-51 34.53361110727978 38.0 33.5 40.0 24.5 41.0 52-53 34.03131865178885 37.5 33.0 40.0 23.5 41.0 54-55 33.68565940674867 37.0 33.0 40.0 23.0 41.0 56-57 33.333264052347886 37.0 32.0 39.5 22.0 41.0 58-59 32.84054259853159 36.0 31.5 39.0 20.0 40.5 60-61 32.42306172174822 36.0 31.0 39.0 18.5 40.0 62-63 31.941782331826747 35.0 31.0 38.5 17.5 40.0 64-65 31.40297705309127 35.0 30.0 38.0 13.5 40.0 66-67 30.89464335908462 34.5 29.0 38.0 10.0 40.0 68-69 30.39472992068199 34.0 29.0 37.0 8.0 39.0 70-71 29.81283171046192 34.0 28.5 36.5 7.0 39.0 72-73 29.32640666162834 34.0 28.0 36.0 3.5 39.0 74-75 28.75188949223062 33.0 27.0 35.5 2.0 38.0 76-77 27.560031894619343 31.5 26.0 34.5 2.0 36.0 78-79 28.251301634349545 33.0 27.0 35.0 2.0 37.0 80-81 27.890801355878033 33.0 26.0 35.0 2.0 36.0 82-83 27.462737372589174 33.0 26.0 35.0 2.0 36.0 84-85 26.920550959811163 32.0 25.0 35.0 2.0 35.5 86-87 26.34067886170548 32.0 24.5 34.5 2.0 35.0 88-89 25.59417283497317 31.5 22.0 34.0 2.0 35.0 90-91 24.990432327617572 31.0 19.5 34.0 2.0 35.0 92-93 24.185000444698318 31.0 16.5 34.0 2.0 35.0 94-95 23.38604451073435 30.5 4.5 34.0 2.0 35.0 96-97 22.324932260793922 30.0 2.0 34.0 2.0 35.0 98-99 20.992210883075735 29.0 2.0 34.0 2.0 35.0 100-101 17.13425703752921 22.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2879623841968346 1101 2 0.27851488166297855 1101 3 0.3489171461493541 1101 4 0.26209853814409456 1101 5 0.06993020541754902 1101 6 0.1828395189584029 1101 7 0.2297467310987642 1101 8 0.2514972742198225 1101 9 0.2794293904389775 1101 10-11 0.2639282578593338 1101 12-13 0.4083702542808112 1101 14-15 0.4008374313848577 1101 16-17 0.5126026915137913 1101 18-19 0.4430527110052438 1101 20-21 0.34439593971521276 1101 22-23 0.36679384829994177 1101 24-25 0.4867251377978761 1101 26-27 0.3773783313991217 1101 28-29 0.370402685167214 1101 30-31 0.4498837726427638 1101 32-33 0.5081264384365625 1101 34-35 0.567203888935282 1101 36-37 0.5688425037462324 1101 38-39 0.5845713729135227 1101 40-41 0.6545681125688247 1101 42-43 0.6826584640502986 1101 44-45 0.6891156815292092 1101 46-47 0.5364961989225137 1101 48-49 0.5891779423980879 1101 50-51 0.673226375998091 1101 52-53 0.8468417932830548 1101 54-55 0.8340358266828574 1101 56-57 0.7934171827426653 1101 58-59 0.9030329399064669 1101 60-61 0.951430694303312 1101 62-63 0.8385162324177813 1101 64-65 0.8642164681398441 1101 66-67 0.7085896621309722 1101 68-69 0.8107365670369191 1101 70-71 0.7746651598464958 1101 72-73 0.8166685903367039 1101 74-75 0.9436855921122458 1101 76-77 0.8279517897782398 1101 78-79 0.9506874805191963 1101 80-81 0.9335202446554369 1101 82-83 1.0259123985998038 1101 84-85 1.1943453918467135 1101 86-87 1.2684452401142963 1101 88-89 1.2671352415813217 1101 90-91 1.2233404403060035 1101 92-93 1.4828861211682955 1101 94-95 1.3660381229984147 1101 96-97 1.3705314638308366 1101 98-99 1.3455500667329439 1101 100-101 1.5319327763391826 1102 1 0.3461855455620544 1102 2 0.25572475243000525 1102 3 0.17320533721564146 1102 4 0.18591809296698614 1102 5 0.12847803429918514 1102 6 0.14298740118608322 1102 7 0.13341940939807984 1102 8 0.23370615021432428 1102 9 0.30247695805617525 1102 10-11 0.40149136032527366 1102 12-13 0.3278655548004963 1102 14-15 0.44628350487559487 1102 16-17 0.38350034800462396 1102 18-19 0.2863607341189436 1102 20-21 0.3306863892430698 1102 22-23 0.4551047792102523 1102 24-25 0.4365088021026011 1102 26-27 0.31332044656972613 1102 28-29 0.273950743611735 1102 30-31 0.39444160172116227 1102 32-33 0.3815665039385152 1102 34-35 0.23063462981125582 1102 36-37 0.4164436261994453 1102 38-39 0.2736369098392615 1102 40-41 0.21360102970099604 1102 42-43 0.22785775743385983 1102 44-45 0.4046796082352415 1102 46-47 0.5939514177615308 1102 48-49 0.4527487166371955 1102 50-51 0.5184742055356253 1102 52-53 0.46120756567471943 1102 54-55 0.40961394256183326 1102 56-57 0.43537696113795477 1102 58-59 0.6218080141864633 1102 60-61 0.509712339547761 1102 62-63 0.49948409142680816 1102 64-65 0.4863354538654079 1102 66-67 0.42600539615284916 1102 68-69 0.6093508817042377 1102 70-71 0.5794757595303537 1102 72-73 0.5548135800719649 1102 74-75 0.587376110727611 1102 76-77 0.4875264206739054 1102 78-79 0.7181342601891032 1102 80-81 0.6019232084216632 1102 82-83 0.6038500930450361 1102 84-85 0.47587093563747374 1102 86-87 0.43355097022267586 1102 88-89 0.5687111908238691 1102 90-91 0.6546270747172613 1102 92-93 1.151446226198786 1102 94-95 1.1837897840499032 1102 96-97 0.9385230727606171 1102 98-99 1.254812328124185 1102 100-101 1.473410636638139 1103 1 0.2030895675723201 1103 2 0.1777686248570518 1103 3 0.27226887564634694 1103 4 0.1410006651189093 1103 5 0.10377034194369372 1103 6 0.06381849270443496 1103 7 0.2584184384483734 1103 8 0.1903955793942913 1103 9 0.30468476822023405 1103 10-11 0.24545935471603286 1103 12-13 0.3114798076171539 1103 14-15 0.3911888411633697 1103 16-17 0.3028080841693708 1103 18-19 0.3238210093301035 1103 20-21 0.32960591641386827 1103 22-23 0.21117709865115586 1103 24-25 0.49083330851060936 1103 26-27 0.46615155465895697 1103 28-29 0.4203049698839223 1103 30-31 0.37536554710148806 1103 32-33 0.22874873022520603 1103 34-35 0.27573550242316713 1103 36-37 0.3985377601615099 1103 38-39 0.34615468339722355 1103 40-41 0.3931380446339432 1103 42-43 0.38473086440559 1103 44-45 0.38516054163120117 1103 46-47 0.2383219290610583 1103 48-49 0.3278432871382435 1103 50-51 0.36001819162851945 1103 52-53 0.1780678871807666 1103 54-55 0.09476201109689697 1103 56-57 0.1681670718469377 1103 58-59 0.3164167577050492 1103 60-61 0.4537049831149744 1103 62-63 0.4644699621667385 1103 64-65 0.5003933815014321 1103 66-67 0.30596325952495107 1103 68-69 0.30587938697507155 1103 70-71 0.18791465883070302 1103 72-73 0.34467611430764933 1103 74-75 0.3283844691804916 1103 76-77 0.26012171485612967 1103 78-79 0.494430685178191 1103 80-81 0.5987572650909527 1103 82-83 0.8400479782906913 1103 84-85 0.6806628701783772 1103 86-87 0.36360608391699856 1103 88-89 0.2932342473838929 1103 90-91 0.2645376748470305 1103 92-93 0.5141771115651004 1103 94-95 0.4579656548882163 1103 96-97 0.5328947233077912 1103 98-99 0.8181504842725751 1103 100-101 1.152761747068002 1104 1 0.31631059365068737 1104 2 0.26110059502097727 1104 3 0.27787821603430984 1104 4 0.22442883028230654 1104 5 0.16174150151093158 1104 6 0.15808080279560954 1104 7 0.2737063630217307 1104 8 0.3069536441852847 1104 9 0.26579220216490995 1104 10-11 0.3199808151123804 1104 12-13 0.34608730150058875 1104 14-15 0.42623665911165176 1104 16-17 0.4121726429529389 1104 18-19 0.4502533443269954 1104 20-21 0.4076836619937225 1104 22-23 0.5739244131870791 1104 24-25 0.48065580432160715 1104 26-27 0.45691927620714523 1104 28-29 0.42398223126600243 1104 30-31 0.460246891546241 1104 32-33 0.4249389199466407 1104 34-35 0.5197872211519226 1104 36-37 0.48830026838019336 1104 38-39 0.4096774419377667 1104 40-41 0.33120640998059514 1104 42-43 0.6345292577537762 1104 44-45 0.6114613531605357 1104 46-47 0.556095517771773 1104 48-49 0.5437726175062991 1104 50-51 0.6728465272572706 1104 52-53 0.5450977103162487 1104 54-55 0.4629766929441459 1104 56-57 0.44815567981984117 1104 58-59 0.6266654071296927 1104 60-61 0.7350723046970558 1104 62-63 0.557260659670817 1104 64-65 0.5929007432266147 1104 66-67 0.7043425202737019 1104 68-69 0.5266787723036757 1104 70-71 0.7199425885849138 1104 72-73 0.6413651752027469 1104 74-75 0.7536934467863432 1104 76-77 0.7945436549334808 1104 78-79 0.9337385987794562 1104 80-81 0.6131935953542644 1104 82-83 0.8745496332597362 1104 84-85 1.0444857864152368 1104 86-87 0.9848960149129553 1104 88-89 1.047943834924638 1104 90-91 1.1085790904565478 1104 92-93 1.2577606731580993 1104 94-95 1.102531845959323 1104 96-97 0.9726167148763238 1104 98-99 1.1640427990978104 1104 100-101 1.4415888199542692 1105 1 0.19763483864828046 1105 2 0.2156809806186999 1105 3 0.2817739089768523 1105 4 0.17328111038472827 1105 5 0.06267013913237918 1105 6 0.12068667103626751 1105 7 0.2248818516381661 1105 8 0.16783074823079858 1105 9 0.22214042189942518 1105 10-11 0.2935093151818009 1105 12-13 0.2976623690657618 1105 14-15 0.3464064357672001 1105 16-17 0.2542025363016833 1105 18-19 0.38827743853399 1105 20-21 0.34665786614547045 1105 22-23 0.2533902528194929 1105 24-25 0.27663725094270575 1105 26-27 0.17884784602126302 1105 28-29 0.27441619822122476 1105 30-31 0.40147649687525444 1105 32-33 0.3015190730620674 1105 34-35 0.42085147344687357 1105 36-37 0.3389223199718927 1105 38-39 0.22743876657023065 1105 40-41 0.35373539761705786 1105 42-43 0.4288316655600113 1105 44-45 0.4470778025746469 1105 46-47 0.3459218341220591 1105 48-49 0.4441176276935366 1105 50-51 0.5086260371716662 1105 52-53 0.4986383834170809 1105 54-55 0.3895986605838999 1105 56-57 0.3680122511580066 1105 58-59 0.4338213414017815 1105 60-61 0.4947485730978656 1105 62-63 0.4905325896936432 1105 64-65 0.36010039708630615 1105 66-67 0.40194924417837186 1105 68-69 0.4094487665884827 1105 70-71 0.5031512317601035 1105 72-73 0.3314799956263208 1105 74-75 0.5109383378264098 1105 76-77 0.4792790933079125 1105 78-79 0.7219036353792738 1105 80-81 0.6067922598726234 1105 82-83 0.6352559273950469 1105 84-85 0.6209506670581781 1105 86-87 0.7037159412289853 1105 88-89 1.1130719746465019 1105 90-91 1.2989255859473623 1105 92-93 1.2919024744438552 1105 94-95 1.537796020114392 1105 96-97 1.6759337852119067 1105 98-99 1.7459750498816327 1105 100-101 1.6798428133220646 1106 1 -0.05291384404534227 1106 2 0.09979674475710709 1106 3 0.02317691457709259 1106 4 0.04361931282669218 1106 5 -0.01708192090654137 1106 6 -0.06582132736480872 1106 7 0.012773711348302186 1106 8 0.0813398620453043 1106 9 -0.1637373656871759 1106 10-11 0.04290080657823836 1106 12-13 0.08661872890534994 1106 14-15 0.10286752263353094 1106 16-17 0.09008190587697129 1106 18-19 0.04256634353490085 1106 20-21 0.1827951444386713 1106 22-23 0.20834513049165793 1106 24-25 0.19013956889033778 1106 26-27 0.13885561781916778 1106 28-29 0.13631917017635686 1106 30-31 0.09590903307940124 1106 32-33 0.1135348118440973 1106 34-35 0.0889480202106867 1106 36-37 0.07976249410651093 1106 38-39 -0.023841195961146866 1106 40-41 0.13616638837423523 1106 42-43 0.225185493593834 1106 44-45 0.05802924176099822 1106 46-47 -0.5725941624572783 1106 48-49 -0.13948323255179673 1106 50-51 0.3339193401011116 1106 52-53 0.2634733151531563 1106 54-55 0.41701953775429246 1106 56-57 0.17826229003927807 1106 58-59 -0.03297249338779551 1106 60-61 0.19669299315640387 1106 62-63 0.14396376717004955 1106 64-65 0.24715554075538648 1106 66-67 0.12187329980911343 1106 68-69 -0.2816513438833468 1106 70-71 -0.048335146871728796 1106 72-73 0.3643126109744621 1106 74-75 0.42316559517942665 1106 76-77 0.4119816290797971 1106 78-79 0.6393739597226009 1106 80-81 -0.018409889314700223 1106 82-83 0.2654429006318111 1106 84-85 0.4075282506796505 1106 86-87 0.7917517173459458 1106 88-89 0.7623605699341347 1106 90-91 0.7881524658512511 1106 92-93 1.0563547208900594 1106 94-95 1.2980562193736667 1106 96-97 1.3124658699476761 1106 98-99 1.4465028102179218 1106 100-101 1.5019045144456342 1107 1 0.18348068103633963 1107 2 0.16355884515571262 1107 3 0.21849179767698956 1107 4 0.06298059232124587 1107 5 0.011474313887070764 1107 6 0.0016953352161621638 1107 7 0.11024408791257656 1107 8 0.09937917285896702 1107 9 0.1887752090586048 1107 10-11 0.15012027434251962 1107 12-13 0.10601754974351252 1107 14-15 0.1114980743137366 1107 16-17 0.13087159178319752 1107 18-19 0.179395128910933 1107 20-21 0.3135319644323147 1107 22-23 0.2507406446968332 1107 24-25 0.19348844925988118 1107 26-27 0.1702693798981727 1107 28-29 0.23049959879367776 1107 30-31 0.11222753854460166 1107 32-33 0.17784505864153743 1107 34-35 0.2992685313034542 1107 36-37 0.26004073214900814 1107 38-39 0.27057491956437474 1107 40-41 0.36783305718000747 1107 42-43 0.2602479320142663 1107 44-45 0.24864161701356835 1107 46-47 0.27750763768757736 1107 48-49 0.4054837032574241 1107 50-51 0.40829280609335683 1107 52-53 0.3292479952174787 1107 54-55 0.4022325265885698 1107 56-57 0.4243324279646785 1107 58-59 0.32915430180502625 1107 60-61 0.2795434228817939 1107 62-63 0.48244026457766864 1107 64-65 0.4076425386158533 1107 66-67 0.3020699308853061 1107 68-69 0.48326615286413954 1107 70-71 0.7465970284341559 1107 72-73 0.7026537028521389 1107 74-75 0.6880977448160124 1107 76-77 0.5184969025240989 1107 78-79 0.8943971198438128 1107 80-81 0.8031659444132977 1107 82-83 1.0108261710111996 1107 84-85 1.056038490375247 1107 86-87 1.0815641518090295 1107 88-89 1.0966067675628892 1107 90-91 1.3172333168568215 1107 92-93 1.7270637667067597 1107 94-95 1.7678571497144695 1107 96-97 1.5714817819156828 1107 98-99 1.5521057541437777 1107 100-101 1.7316066265579906 1108 1 0.2664059214134262 1108 2 0.12937925919936788 1108 3 0.1662139311606552 1108 4 0.1815267415899342 1108 5 0.13632883485546898 1108 6 0.15004323211240234 1108 7 0.20717352510133935 1108 8 0.21662618967752678 1108 9 0.22774291866429053 1108 10-11 0.2973635328222741 1108 12-13 0.28829917459866294 1108 14-15 0.36219086143516677 1108 16-17 0.4211954353110272 1108 18-19 0.3732774929060412 1108 20-21 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2316 72-73 -1.0053006656307168 2316 74-75 -1.0437667906671457 2316 76-77 -0.9619864787233503 2316 78-79 -1.2146407442437912 2316 80-81 -1.2957283933090373 2316 82-83 -1.3026986823761533 2316 84-85 -1.5609483994586633 2316 86-87 -1.3701872427020483 2316 88-89 -1.448906811513588 2316 90-91 -1.4845809969027712 2316 92-93 -1.7479023864888248 2316 94-95 -1.5377303296392633 2316 96-97 -1.6514566890840534 2316 98-99 -1.6246788619578716 2316 100-101 -2.1265323505239415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 16.0 4 26.0 5 93.0 6 213.0 7 481.0 8 1127.0 9 2096.0 10 3337.0 11 5289.0 12 7369.0 13 9991.0 14 12855.0 15 17054.0 16 21666.0 17 26941.0 18 25297.0 19 20707.0 20 20001.0 21 21391.0 22 22907.0 23 25741.0 24 29191.0 25 34187.0 26 40615.0 27 48393.0 28 57671.0 29 67861.0 30 79988.0 31 95873.0 32 115047.0 33 140737.0 34 179503.0 35 233180.0 36 294009.0 37 305298.0 38 131490.0 39 4903.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.91633651075698 19.244276689835445 15.379906798532444 22.459480000875132 2 16.142783112446484 20.84243616202694 38.58535023148269 24.42943049404388 3 19.330203481583546 24.602565742819788 30.08400755463963 25.983223220957036 4 13.366442351640796 17.123501045635557 35.852006302825764 33.65805029989789 5 13.667625621314109 37.86226074113268 33.51051630333928 14.959597334213933 6 32.386260676921204 36.26540927226904 16.553478658523535 14.79485139228622 7 28.941822521139816 33.26942677673624 19.779800613445868 18.008950088678077 8 27.57110535830556 34.18712239286694 18.98904615302664 19.25272609580086 9 27.03423320930927 16.4497474256248 19.49757175694835 37.018447608117576 10-11 25.409633735052072 26.621353414355625 26.37890959972871 21.590103250863596 12-13 26.71069734878949 24.001913867405705 26.971143121991446 22.31624566181335 14-15 23.033446545122136 25.2621936516105 25.669770359691974 26.034589443575396 16-17 22.117748504315475 28.479645420867715 26.69497833344193 22.707627741374875 18-19 22.29679308306251 26.43617342568473 29.51819434410328 21.748839147149486 20-21 22.851595848657986 25.401500749804168 29.85285955285703 21.894043848680816 22-23 22.636357107491907 25.428919849192578 29.970359787096516 21.964363256219 24-25 22.513768105084942 25.842918150972682 29.75535022011607 21.887963523826308 26-27 21.786887121103845 26.09298762833201 29.77105172852104 22.349073522043106 28-29 22.005659330559077 26.556214835778825 29.546875442172354 21.891250391489745 30-31 22.057929642390356 26.03992074257699 29.30531800947056 22.596831605562098 32-33 21.73347681075843 26.13560994750391 29.337954733268727 22.79295850846893 34-35 22.70786663562153 26.185603445343958 29.09732150098308 22.00920841805143 36-37 22.860436915720012 25.529732123785653 29.20516276230904 22.4046681981853 38-39 22.159679099797593 26.044793707182606 29.199954053833594 22.595573139186207 40-41 22.499830560671764 25.99881808637006 29.294097922852192 22.20725343010598 42-43 22.308235613063403 25.9573177837019 28.84559682095015 22.888849782284552 44-45 22.248412042317632 26.1021049417186 29.227577618952942 22.421905397010825 46-47 22.997585311923764 26.58204398565741 28.192660432646278 22.227710269772547 48-49 22.524897160541798 26.832287856311552 27.85409519587891 22.78871978726774 50-51 22.657642660781722 26.875398327736928 28.03361648292066 22.433342528560694 52-53 23.09879579500882 26.532603996387245 27.913332816500354 22.455267392103583 54-55 22.37744343808546 26.88001868235198 27.964218159265148 22.778319720297414 56-57 22.515860510266346 26.975138272639544 27.747106012749285 22.76189520434482 58-59 22.505807969626108 26.49247633937714 27.783823410131497 23.21789228086525 60-61 22.064456048348934 26.966577235536484 28.112638516391296 22.85632819972329 62-63 22.255047852300837 26.875985948135767 28.362153005118785 22.50681319444461 64-65 21.99009052763469 27.032138713585706 27.92086121839132 23.056909540388283 66-67 21.899696991965687 27.02376951888051 27.890818174013322 23.185715315140484 68-69 22.534565730365543 26.531682071629568 28.00255257551587 22.931199622489018 70-71 22.341313259896985 26.82337822255061 27.540128125584857 23.29518039196755 72-73 22.21070709885953 26.9111683009528 27.590819524301217 23.28730507588646 74-75 22.246388808016455 27.377578165584882 27.30712400503122 23.06890902136745 76-77 22.273794047276887 26.82310948602231 27.803698515150188 23.099397951550614 78-79 22.32991255816432 27.125737691620223 27.62937450030603 22.91497524990943 80-81 22.642581639607087 27.1767687639971 27.174461434897047 23.006188161498763 82-83 22.46901773830382 27.163120314300905 27.393089043596998 22.97477290379828 84-85 22.274426679231254 27.4501838427232 27.303694764234404 22.971694713811143 86-87 22.596572106998817 27.39077533092871 26.973882923845373 23.038769638227098 88-89 22.335171445687386 27.39539503231274 26.660649901120838 23.608783620879034 90-91 22.16351389217278 27.392030774339432 26.974073874970205 23.47038145851758 92-93 22.41169047871069 27.023587660155968 27.1163779211033 23.448343940030043 94-95 22.090888504278343 26.640356577744246 27.459963976856127 23.808790941121284 96-97 22.386579419028937 26.31774272668058 27.383256009139018 23.91242184515146 98-99 22.057615675331512 26.847231567656877 26.87299958790202 24.222153169109593 100-101 22.163539316516186 26.325348113624443 27.39788484672916 24.113227723130215 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1986.0 1 1306.5 2 846.0 3 1061.0 4 889.5 5 812.0 6 1385.5 7 3063.5 8 4600.0 9 4793.0 10 3807.0 11 2733.0 12 2480.0 13 2554.5 14 2705.0 15 2831.0 16 2967.5 17 3138.0 18 3413.0 19 3737.0 20 4088.0 21 4704.0 22 5471.0 23 6373.5 24 7720.0 25 9557.0 26 12028.0 27 14822.5 28 17128.5 29 19371.0 30 22587.0 31 27755.0 32 35404.5 33 38261.5 34 37154.5 35 39002.5 36 43144.5 37 47542.0 38 50674.5 39 53027.0 40 55229.0 41 58546.0 42 60738.0 43 62353.0 44 64488.0 45 68190.5 46 70318.5 47 73103.0 48 77303.5 49 76060.0 50 73829.5 51 72675.5 52 72304.0 53 75043.5 54 77813.5 55 74792.0 56 69767.5 57 64250.5 58 59390.5 59 53553.5 60 43877.0 61 36847.0 62 31532.0 63 23074.5 64 15191.0 65 11149.0 66 8473.5 67 6641.5 68 5223.5 69 4003.0 70 3036.5 71 2109.5 72 1490.0 73 1166.0 74 905.0 75 695.0 76 485.5 77 314.5 78 231.0 79 161.5 80 97.0 81 64.5 82 42.5 83 22.0 84 11.5 85 7.5 86 5.0 87 3.5 88 3.0 89 1.0 90 1.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.329292206745241E-4 2 0.05212720369418264 3 0.0 4 0.0 5 0.04542105796345293 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006444558485914002 26-27 0.002068917299905971 28-29 1.6646461033726205E-4 30-31 0.0015695234688941852 32-33 5.469551482510039E-4 34-35 0.01110556757535734 36-37 0.023590413350651995 38-39 0.004209176575670769 40-41 0.0012603749068392698 42-43 0.0016170847861334027 44-45 0.0077524947099924905 46-47 0.0 48-49 0.0 50-51 4.2805185515295955E-4 52-53 0.0 54-55 0.001426839517176532 56-57 0.0 58-59 0.02285321293344412 60-61 0.0 62-63 0.009250676203027849 64-65 0.015124498882071237 66-67 0.0061591905824786965 68-69 0.017050732230259557 70-71 1.1890329309804431E-4 72-73 0.0 74-75 0.022496503054149988 76-77 0.050272312321853134 78-79 0.0303203397400013 80-81 0.026230066457428577 82-83 0.03526671673287995 84-85 0.016384873788910508 86-87 0.009892753985757289 88-89 0.08335120846172907 90-91 0.02558798867469914 92-93 0.023614194009271602 94-95 0.026967266874636454 96-97 0.03766856325346044 98-99 0.052650378183814024 100-101 0.013792781999373142 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2102549.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.09376020576496 #Duplication Level Percentage of deduplicated Percentage of total 1 70.96950490706621 31.293123312940374 2 14.480872670955305 12.770322542466367 3 5.537199264649981 7.32467809761043 4 2.737633666640445 4.828502497122914 5 1.5427485203195188 3.4012791656383787 6 0.9779139272589196 2.5871941326259607 7 0.6803623856321533 2.099981511956045 8 0.49008063707147625 1.728759847401456 9 0.37980366304345275 1.5072274479158245 >10 1.9834688759652825 15.981567062033871 >50 0.14201697433778587 4.275626229006044 >100 0.06857035059111541 5.677844312254154 >500 0.00485809935252081 1.557748703411229 >1k 0.004642183825742105 3.6821115987520456 >5k 2.159155267787026E-4 0.7672593082013868 >10k+ 1.079577633893513E-4 0.5167742306634976 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10856 0.5163256599489476 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9192 0.4371836280628894 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6926 0.32940968319882197 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3338 0.1587596769445088 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3317 0.15776088928248522 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3176 0.1510547435517555 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3006 0.1429693196210885 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2879 0.13692903233170783 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2808 0.13355217880772338 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2760 0.13126923558024092 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2367 0.11257763790522837 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2183 0.10382635553321232 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2146 0.10206658679536125 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2131 0.101353167036773 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2120 0.10082999254714159 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.512263447843546E-5 2 0.0 0.0 0.0 0.0 9.512263447843546E-5 3 0.0 0.0 0.0 0.0 9.512263447843546E-5 4 0.0 0.0 0.0 0.0 9.512263447843546E-5 5 0.0 0.0 0.0 0.0 9.512263447843546E-5 6 0.0 0.0 0.0 0.0 9.512263447843546E-5 7 0.0 0.0 0.0 0.0 9.512263447843546E-5 8 0.0 0.0 0.0 0.0 9.512263447843546E-5 9 0.0 0.0 0.0 0.0 9.512263447843546E-5 10-11 0.0 0.0 0.0 4.756131723921773E-5 9.512263447843546E-5 12-13 0.0 0.0 0.0 4.756131723921773E-5 2.853679034353064E-4 14-15 0.0 0.0 0.0 4.756131723921773E-5 2.853679034353064E-4 16-17 0.0 0.0 0.0 4.756131723921773E-5 2.853679034353064E-4 18-19 0.0 0.0 0.0 4.756131723921773E-5 3.091485620549153E-4 20-21 0.0 0.0 0.0 7.13419758588266E-5 3.56709879294133E-4 22-23 0.0 0.0 0.0 3.56709879294133E-4 3.8049053791374185E-4 24-25 0.0 0.0 0.0 8.79884368925528E-4 3.8049053791374185E-4 26-27 0.0 0.0 0.0 0.0023542852033412776 4.2805185515295955E-4 28-29 0.0 0.0 0.0 0.005802480703184563 4.756131723921773E-4 30-31 0.0 0.0 0.0 0.017264758157836034 4.756131723921773E-4 32-33 0.0 0.0 0.0 0.03643196900524078 4.756131723921773E-4 34-35 0.0 0.0 0.0 0.0657297404245989 5.707358068706128E-4 36-37 0.0 0.0 0.0 0.11340996095691468 5.945164654902217E-4 38-39 0.0 0.0 0.0 0.18720134465356097 6.658584413490482E-4 40-41 0.0 0.0 0.0 0.26565373743965065 6.658584413490482E-4 42-43 0.0 0.0 0.0 0.346008582915309 6.658584413490482E-4 44-45 0.0 0.0 0.0 0.44771845983137615 7.372004172078748E-4 46-47 0.0 0.0 0.0 0.567739443884542 7.847617344470926E-4 48-49 0.0 0.0 0.0 0.6970824461165946 8.561037103059191E-4 50-51 0.0 0.0 0.0 0.84571156248915 9.036650275451369E-4 52-53 0.0 0.0 0.0 0.9866595261275719 9.036650275451369E-4 54-55 0.0 0.0 0.0 1.1325300861002525 9.274456861647457E-4 56-57 0.0 0.0 0.0 1.3071990236612798 9.512263447843546E-4 58-59 0.0 0.0 0.0 1.4922125477218366 9.512263447843546E-4 60-61 0.0 0.0 0.0 1.6994134262744887 9.750070034039635E-4 62-63 0.0 0.0 0.0 1.9057344204582152 9.987876620235724E-4 64-65 0.0 0.0 0.0 2.1146712870900988 0.0010463489792627901 66-67 0.0 0.0 0.0 2.3617047688305957 0.0010463489792627901 68-69 0.0 0.0 0.0 2.6390585903110937 0.0010463489792627901 70-71 0.0 0.0 0.0 2.9320125238460557 0.0010463489792627901 72-73 0.0 0.0 0.0 3.2347403080736763 0.001070129637882399 74-75 0.0 0.0 0.0 3.5412729976804345 0.0011176909551216167 76-77 0.0 0.0 0.0 3.869208280044841 0.0011890329309804434 78-79 0.0 0.0 0.0 4.24560854467601 0.001236594248219661 80-81 0.0 0.0 0.0 4.642983350209674 0.001236594248219661 82-83 0.0 0.0 0.0 5.056338758335715 0.001236594248219661 84-85 0.0 0.0 0.0 5.47982472703371 0.001236594248219661 86-87 0.0 0.0 0.0 5.91394065013467 0.001236594248219661 88-89 0.0 0.0 0.0 6.369126236772603 0.0012603749068392698 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7940 0.0 37.11577 1 GTATCAA 12145 0.0 33.501442 1 AGGTAAG 1315 0.0 28.555424 2 TAAGGTG 1435 0.0 28.140179 5 TATCAAC 15005 0.0 26.989252 2 ATCAACG 15275 0.0 26.405008 3 TCAACGC 15400 0.0 26.190683 4 CAACGCA 15675 0.0 25.791815 5 GGTAAGG 1530 0.0 25.150896 3 AACGCAG 16075 0.0 25.077791 6 GTAAGGT 1540 0.0 24.987576 4 GTGTAAG 860 0.0 24.871462 1 TAAGGTA 780 0.0 23.753624 4 TACATGG 10360 0.0 23.62842 2 GTACATG 10625 0.0 23.441725 1 AAGGTAA 1595 0.0 22.946545 1 ACATGGG 10545 0.0 22.525959 3 GAGTACT 9970 0.0 22.360064 12-13 ACGCAGA 18030 0.0 22.33226 7 CGCAGAG 18105 0.0 22.056168 8 >>END_MODULE