FastQCFastQC Report
Thu 26 May 2016
SRR938514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103221
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114590.5448309996904748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91010.43271724654708177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64450.30643474936775544No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32500.15452489300934139No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA32370.153906793437304No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31250.1485816278935975No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28830.1370754666295173No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA28410.13507852955062735No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26790.12737605796062323No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA25310.12033923206358248No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24450.11625026566395068No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA22730.10807233286468707No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA22130.10521956560912998No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21820.10374563586042553No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21640.10288980568375838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78700.038.1525421
GTATCAA122600.034.3107831
TATCAAC152150.027.4247912
ATCAACG155500.026.8651733
TCAACGC157000.026.6084964
CAACGCA159150.026.2185525
AACGCAG164250.025.4574836
GTATAGG4600.024.8268851
GTACATG101000.024.499141
TACATGG100500.024.2589552
GAGTACT100700.022.94783612-13
ACGCAGA182100.022.8794277
ACATGGG104100.022.8270823
CGCAGAG182050.022.8074258
GTGTAAG9700.022.5660171
TAAGGTA6850.022.201924
GTACTTT104700.021.70947814-15
GTGTACG2457.374183E-921.3646321
AGTACTT105200.021.26637812-13
AGAGTAC153650.021.11529410-11
Produced by FastQC (version 0.11.5)