##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938514_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2103221 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.0035583516901 31.0 31.0 34.0 28.0 34.0 2 31.089960113559155 31.0 31.0 34.0 27.0 34.0 3 31.135844497558743 31.0 31.0 34.0 27.0 34.0 4 34.784804354844304 37.0 35.0 37.0 32.0 37.0 5 34.8113869155928 37.0 35.0 37.0 32.0 37.0 6 34.84669038584153 37.0 35.0 37.0 32.0 37.0 7 34.76957247954447 37.0 35.0 37.0 32.0 37.0 8 34.72829436374019 37.0 35.0 37.0 31.0 37.0 9 36.21755393275362 39.0 35.0 39.0 32.0 39.0 10-11 36.05979756763554 39.0 35.0 39.0 30.0 39.0 12-13 35.90747667506173 38.0 35.0 39.0 30.0 39.0 14-15 36.851712682594936 39.0 36.0 41.0 30.5 41.0 16-17 36.71211679609513 39.0 36.0 41.0 30.0 41.0 18-19 36.735076104698464 39.0 36.0 40.5 30.0 41.0 20-21 36.820241429692835 39.0 36.0 41.0 30.0 41.0 22-23 36.74215952579401 39.0 36.0 41.0 30.0 41.0 24-25 36.60988526645559 39.0 36.0 41.0 30.0 41.0 26-27 36.40952234691457 39.0 35.5 41.0 29.5 41.0 28-29 36.21972774140235 39.0 35.0 40.5 29.0 41.0 30-31 35.947856406911114 38.5 35.0 40.0 28.0 41.0 32-33 35.621251166662944 38.0 34.5 40.0 27.0 41.0 34-35 35.346684680307014 38.0 34.0 40.0 26.5 41.0 36-37 35.0297617321242 38.0 34.0 40.0 25.0 41.0 38-39 34.74387023522492 38.0 33.5 40.0 24.5 41.0 40-41 34.617221632914465 38.0 33.0 40.0 24.0 41.0 42-43 34.3882711327055 38.0 33.0 40.0 24.0 41.0 44-45 34.04437954927228 38.0 33.0 40.0 23.0 41.0 46-47 33.60807328378711 38.0 33.0 40.0 20.5 41.0 48-49 33.17142207119461 37.5 32.0 40.0 17.5 41.0 50-51 32.382460758997745 36.5 30.5 39.5 15.0 40.5 52-53 32.41930947817657 37.0 31.0 39.0 14.5 40.0 54-55 32.53224910744045 37.0 31.0 40.0 12.5 41.0 56-57 32.2133472897047 36.5 31.0 39.5 8.5 41.0 58-59 31.85812071104273 36.0 31.0 39.0 7.5 41.0 60-61 31.389091778752686 35.5 30.0 39.0 4.5 40.5 62-63 30.856715485438762 35.0 29.0 39.0 2.0 40.0 64-65 30.34853089618257 35.0 29.0 38.0 2.0 40.0 66-67 29.79621518613593 34.5 28.0 38.0 2.0 40.0 68-69 29.23214869003305 34.0 27.5 37.0 2.0 39.5 70-71 28.58908526493412 34.0 26.0 36.5 2.0 39.0 72-73 28.044206956853323 33.5 26.0 36.0 2.0 39.0 74-75 27.44162810280042 33.0 25.0 35.5 2.0 38.0 76-77 26.719407993739125 32.5 23.5 35.0 2.0 37.0 78-79 25.955263379359565 32.0 21.0 35.0 2.0 36.5 80-81 25.308392460896883 31.5 19.0 35.0 2.0 36.0 82-83 24.651324563609815 31.0 16.5 34.5 2.0 36.0 84-85 24.006285834917016 31.0 9.0 34.0 2.0 35.0 86-87 23.350676890350563 30.0 4.5 34.0 2.0 35.0 88-89 22.550942340343692 29.5 2.0 34.0 2.0 35.0 90-91 21.713992252835055 29.0 2.0 34.0 2.0 35.0 92-93 20.876808713872677 29.0 2.0 34.0 2.0 35.0 94-95 19.925716080240736 27.0 2.0 33.5 2.0 35.0 96-97 18.698440867602596 25.0 2.0 33.0 2.0 35.0 98-99 17.23315143772338 21.0 2.0 32.5 2.0 34.0 100-101 13.939336855232998 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5237667089683811 1101 2 0.36091663086873993 1101 3 0.483078737241879 1101 4 0.4329217506518077 1101 5 0.3428222588122054 1101 6 0.4986904211084564 1101 7 0.3955314678035009 1101 8 0.4832851286855444 1101 9 0.3634299043014977 1101 10-11 0.6355631221442977 1101 12-13 0.6665920003304464 1101 14-15 0.7128277378137327 1101 16-17 0.6298067145938191 1101 18-19 0.664196473763532 1101 20-21 0.6968552309125258 1101 22-23 0.8190977978493876 1101 24-25 0.7355009084417645 1101 26-27 0.7058687970896997 1101 28-29 0.6810760102417532 1101 30-31 0.5591972565185657 1101 32-33 0.5936347913343667 1101 34-35 0.44914783794114754 1101 36-37 0.6781818496081087 1101 38-39 0.6413424121869085 1101 40-41 0.49556709239951147 1101 42-43 0.6088411632658719 1101 44-45 0.5570501888952535 1101 46-47 0.4977857578014877 1101 48-49 0.4067474306456731 1101 50-51 0.29770040314336654 1101 52-53 0.29294344976181463 1101 54-55 0.33309000504182507 1101 56-57 0.2847830073723543 1101 58-59 0.284948556790912 1101 60-61 0.23991400632011306 1101 62-63 0.24501104991358957 1101 64-65 0.1340068101536076 1101 66-67 0.16546298138466398 1101 68-69 0.21582237278688865 1101 70-71 0.3914209617775626 1101 72-73 0.23358535412343073 1101 74-75 0.25438620214911367 1101 76-77 0.3420919828896736 1101 78-79 0.35149483889438926 1101 80-81 0.4255555208172588 1101 82-83 0.4226918448901067 1101 84-85 0.6822584294126628 1101 86-87 0.8700438285244516 1101 88-89 0.9534474198416731 1101 90-91 0.8072454538597817 1101 92-93 0.8669985748607907 1101 94-95 0.5579287613172248 1101 96-97 0.8181574478631717 1101 98-99 1.0437206443429083 1101 100-101 0.8939986727799187 1102 1 0.5075936965237524 1102 2 0.5950779373072592 1102 3 0.563357530119216 1102 4 0.5869972647276569 1102 5 0.44750336920311895 1102 6 0.5004050839793592 1102 7 0.4099954701119657 1102 8 0.5145051826503177 1102 9 0.6247326820015147 1102 10-11 0.6793174424486992 1102 12-13 0.692720689951166 1102 14-15 0.7369728558966671 1102 16-17 0.7392608865273971 1102 18-19 0.8700342144354423 1102 20-21 0.6625520139142722 1102 22-23 0.806344016977377 1102 24-25 0.6643428030661696 1102 26-27 0.5864870770648096 1102 28-29 0.43644570447725783 1102 30-31 0.5890703445483396 1102 32-33 0.5028771741397122 1102 34-35 0.5811304458222466 1102 36-37 0.539464142874138 1102 38-39 0.6128823732530222 1102 40-41 0.7076704857630958 1102 42-43 0.6447455846734798 1102 44-45 0.5651850125407023 1102 46-47 0.5666625809430244 1102 48-49 0.5035219802034661 1102 50-51 0.5423254599398888 1102 52-53 0.5735216491558432 1102 54-55 0.3689648168865176 1102 56-57 0.463580083885617 1102 58-59 0.7138942194744651 1102 60-61 0.6380017938289697 1102 62-63 0.2738342097600075 1102 64-65 0.28603280411972065 1102 66-67 0.35715031193721103 1102 68-69 0.3560929665070738 1102 70-71 0.4129160239791787 1102 72-73 0.2816395213194802 1102 74-75 0.4577780583349309 1102 76-77 0.5935339686038681 1102 78-79 0.4776408635683076 1102 80-81 0.5014739017878931 1102 82-83 0.40118870735314616 1102 84-85 0.34169071718746835 1102 86-87 0.2704757512940965 1102 88-89 0.29582979450708535 1102 90-91 0.5336814907436072 1102 92-93 0.5678868079128137 1102 94-95 0.6729251704847776 1102 96-97 0.8636709799717828 1102 98-99 0.8606791715740165 1102 100-101 0.6283684574591781 1103 1 0.6145756141078316 1103 2 0.6477500123673927 1103 3 0.5938092376167283 1103 4 0.45724785499883325 1103 5 0.6497421494141093 1103 6 0.6240176311636958 1103 7 0.44178588121272355 1103 8 0.5625710045195333 1103 9 0.5392375110714482 1103 10-11 0.5547364796021697 1103 12-13 0.546932853736152 1103 14-15 0.8411189474333938 1103 16-17 0.7959498451720961 1103 18-19 0.7175817104646711 1103 20-21 0.617679297657098 1103 22-23 0.782704448433023 1103 24-25 0.8087051273049752 1103 26-27 0.9279062110727239 1103 28-29 0.687054007067033 1103 30-31 0.7292904668837465 1103 32-33 0.8223729437126792 1103 34-35 0.9075987051263255 1103 36-37 0.9829874397170428 1103 38-39 0.8324752291042401 1103 40-41 1.0197049855227647 1103 42-43 0.9496587067507747 1103 44-45 0.8599226219978178 1103 46-47 0.7799740653558942 1103 48-49 0.9706957932341922 1103 50-51 1.027088787343466 1103 52-53 1.1961701597353809 1103 54-55 1.1355313061756007 1103 56-57 1.0138771642907827 1103 58-59 0.9283251970294764 1103 60-61 1.0329436991928986 1103 62-63 1.169141508824385 1103 64-65 1.0812966732384517 1103 66-67 1.1077208773559484 1103 68-69 0.8166237494773831 1103 70-71 0.9994528953848132 1103 72-73 0.9984914736320611 1103 74-75 0.9191053145669699 1103 76-77 0.7105645349396319 1103 78-79 0.7789424224612205 1103 80-81 0.8150548527852095 1103 82-83 0.9348470440222911 1103 84-85 1.0170741551802749 1103 86-87 1.0394208415207125 1103 88-89 1.0388539560850276 1103 90-91 1.1800458169844141 1103 92-93 1.2373370095297673 1103 94-95 1.1928406083106609 1103 96-97 1.2845063804777972 1103 98-99 1.50385232413986 1103 100-101 1.230774517662459 1104 1 0.43051308779931574 1104 2 0.4568821878097289 1104 3 0.5025456356579419 1104 4 0.4353440844319607 1104 5 0.3848554834579261 1104 6 0.44672122926836977 1104 7 0.42474745240959066 1104 8 0.3794192090682742 1104 9 0.2863545843917308 1104 10-11 0.37847283141338295 1104 12-13 0.4255718232842156 1104 14-15 0.4801607602719926 1104 16-17 0.5288543588252708 1104 18-19 0.5845972432556081 1104 20-21 0.5748890596796912 1104 22-23 0.603489623496273 1104 24-25 0.6894782193080147 1104 26-27 0.6060364560478888 1104 28-29 0.5000118360676424 1104 30-31 0.44204880129982627 1104 32-33 0.5207115350994016 1104 34-35 0.5188095756954922 1104 36-37 0.5732351984139967 1104 38-39 0.6940783246645523 1104 40-41 0.6216424845190858 1104 42-43 0.5728904703568745 1104 44-45 0.6720526908123716 1104 46-47 0.8158815090173519 1104 48-49 0.8120084233168825 1104 50-51 0.5709285678142635 1104 52-53 0.6872963126262377 1104 54-55 0.8573139784930248 1104 56-57 0.9194670030356988 1104 58-59 0.8738347561753947 1104 60-61 1.1095685739461203 1104 62-63 1.0665016789118233 1104 64-65 1.1762864126132158 1104 66-67 1.1209498330982726 1104 68-69 1.1202421466786703 1104 70-71 1.0133948879364283 1104 72-73 1.0274370453583792 1104 74-75 0.9304428553162758 1104 76-77 0.8902439713035655 1104 78-79 1.0866439012856937 1104 80-81 1.0993345140702182 1104 82-83 1.063427861105783 1104 84-85 1.1028781166526826 1104 86-87 1.3300652778870727 1104 88-89 1.1616679465460784 1104 90-91 0.9465174417448061 1104 92-93 1.0183973601088354 1104 94-95 0.9184210745328372 1104 96-97 0.8632866678142328 1104 98-99 0.690062963559054 1104 100-101 0.43887566694007774 1105 1 0.5501427602012932 1105 2 0.586557240648304 1105 3 0.4831459427429081 1105 4 0.57984413548985 1105 5 0.4441681543664302 1105 6 0.44662971693367837 1105 7 0.311076224187282 1105 8 0.42814710693774316 1105 9 0.524064443266802 1105 10-11 0.6174439238885654 1105 12-13 0.567558742783099 1105 14-15 0.7171265339672956 1105 16-17 0.7107330494289883 1105 18-19 0.6619207863826944 1105 20-21 0.5269569964084795 1105 22-23 0.5617861617252586 1105 24-25 0.7302582593844207 1105 26-27 0.8541219004463088 1105 28-29 0.8323572050018058 1105 30-31 0.8360682973457827 1105 32-33 0.7147916782366792 1105 34-35 0.8293098899954074 1105 36-37 0.8898153202291326 1105 38-39 0.6410337876627992 1105 40-41 0.7406232722305219 1105 42-43 0.8095580798080491 1105 44-45 0.8606075174195524 1105 46-47 0.5971991852125953 1105 48-49 0.4670342369410676 1105 50-51 0.5369802577612504 1105 52-53 0.6885704547672518 1105 54-55 0.5827548455043967 1105 56-57 0.644376084263385 1105 58-59 0.8370365399513453 1105 60-61 0.8780387942879955 1105 62-63 0.7320201769662091 1105 64-65 0.7040773486507064 1105 66-67 0.4442811218109277 1105 68-69 0.2898601437664112 1105 70-71 0.4253261370475272 1105 72-73 0.4525507961124333 1105 74-75 0.5520277106472626 1105 76-77 0.6656775426842216 1105 78-79 0.7038410052545387 1105 80-81 0.6701731289561188 1105 82-83 0.7267837175379128 1105 84-85 0.5945858774288375 1105 86-87 0.7052313889102031 1105 88-89 0.8353214762367607 1105 90-91 0.8644143960898347 1105 92-93 1.0298136967432967 1105 94-95 0.9674836724274094 1105 96-97 1.068448217051209 1105 98-99 0.8986286794425311 1105 100-101 0.7934687908830451 1106 1 0.3578973011795199 1106 2 0.4580300038973846 1106 3 0.1623734749309662 1106 4 0.5045865398375184 1106 5 0.5231328478048027 1106 6 0.1641635463096165 1106 7 0.3438316770085237 1106 8 0.28503722073575233 1106 9 0.5359717453056874 1106 10-11 0.2799638704545586 1106 12-13 0.2455316929599931 1106 14-15 0.49046492802937536 1106 16-17 0.413847791195046 1106 18-19 0.029075175083129068 1106 20-21 0.2034713396991421 1106 22-23 0.40800691436049163 1106 24-25 0.4559225370223885 1106 26-27 0.16155957597609216 1106 28-29 0.2534518953399214 1106 30-31 0.3208875928558683 1106 32-33 0.5455196481093907 1106 34-35 0.2980434768210998 1106 36-37 0.3026578312624366 1106 38-39 0.2504066220356336 1106 40-41 0.3650171245849094 1106 42-43 0.07566909213616668 1106 44-45 0.3427186391688295 1106 46-47 0.2033618762437115 1106 48-49 0.4961322874206928 1106 50-51 0.12339037739505443 1106 52-53 0.3690206544608756 1106 54-55 -0.035142011372713 1106 56-57 -0.4334992987106858 1106 58-59 -0.8085198210655413 1106 60-61 0.15891847204267506 1106 62-63 0.045174994232869636 1106 64-65 0.18851879921057701 1106 66-67 -0.37004655353648275 1106 68-69 -0.6886644714358319 1106 70-71 -0.5135184894615747 1106 72-73 0.04238705190264014 1106 74-75 0.2145286224902776 1106 76-77 0.258069010569109 1106 78-79 0.4562341372529346 1106 80-81 0.4615679524233123 1106 82-83 0.5954951921704392 1106 84-85 0.45358326043938035 1106 86-87 0.493292742267613 1106 88-89 0.26643321803929254 1106 90-91 0.4014631477767452 1106 92-93 0.642234936302696 1106 94-95 0.5267964391414921 1106 96-97 0.4803195012909178 1106 98-99 0.15093017121770558 1106 100-101 -0.1762801331421926 1107 1 0.6042624075920919 1107 2 0.4921454928388229 1107 3 0.4477947783111311 1107 4 0.5440548247171932 1107 5 0.505769514935146 1107 6 0.4398103678425116 1107 7 0.4482507547662067 1107 8 0.4912646167574479 1107 9 0.5487882010018836 1107 10-11 0.6135190415492886 1107 12-13 0.4437446745986122 1107 14-15 0.5747056082352415 1107 16-17 0.6585988866186057 1107 18-19 0.623951461771135 1107 20-21 0.6780305621222809 1107 22-23 0.7504228449488863 1107 24-25 0.6367352369806554 1107 26-27 0.6827455893298264 1107 28-29 0.7312421825843245 1107 30-31 0.8599398775964389 1107 32-33 0.7847679788925319 1107 34-35 0.7723870920666158 1107 36-37 1.0020888195779136 1107 38-39 0.9894384762573623 1107 40-41 0.980160920662243 1107 42-43 1.0111290830945734 1107 44-45 0.9976423942112547 1107 46-47 1.0468188939095882 1107 48-49 1.1155117212779757 1107 50-51 1.1358842917085923 1107 52-53 1.0673562192125843 1107 54-55 0.9573452086857017 1107 56-57 0.9787516228361213 1107 58-59 1.1507017326971543 1107 60-61 1.1652315023667796 1107 62-63 1.1744067197857966 1107 64-65 1.0876242033001589 1107 66-67 1.1020266659655356 1107 68-69 0.8980648198425065 1107 70-71 1.0825262490277865 1107 72-73 1.1986844333186895 1107 74-75 1.1791603515207427 1107 76-77 1.1528096733873063 1107 78-79 1.310450648589697 1107 80-81 1.3376307740953095 1107 82-83 1.359893926347656 1107 84-85 1.400657784017067 1107 86-87 1.2924942132649697 1107 88-89 1.5174412685331014 1107 90-91 1.547665244730421 1107 92-93 1.6789590112783515 1107 94-95 1.6968179129208742 1107 96-97 1.931627054497202 1107 98-99 1.6216041175245657 1107 100-101 1.0792221968063398 1108 1 0.20416931177266662 1108 2 0.24321209551757406 1108 3 0.3730049860327256 1108 4 0.3900082487715366 1108 5 0.38443717275482214 1108 6 0.38345833041287847 1108 7 0.3488447869036406 1108 8 0.1743640561210711 1108 9 0.10315710782278131 1108 10-11 0.22207173551471726 1108 12-13 0.3361023241641732 1108 14-15 0.36698133512888376 1108 16-17 0.34564180799674915 1108 18-19 0.4257899186728835 1108 20-21 0.4834787496398647 1108 22-23 0.5027709581712188 1108 24-25 0.37682865438883084 1108 26-27 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-1.2179884881027903 2316 54-55 -1.3869522838154111 2316 56-57 -1.22831691618223 2316 58-59 -1.2470316986538315 2316 60-61 -1.2151981476136342 2316 62-63 -1.2616139605067538 2316 64-65 -1.1483740742364503 2316 66-67 -1.038362149290876 2316 68-69 -1.2260685825324522 2316 70-71 -1.6401135468522554 2316 72-73 -1.326342262180905 2316 74-75 -1.273248548410038 2316 76-77 -1.2074212762212504 2316 78-79 -1.2350785930434753 2316 80-81 -1.126589207553213 2316 82-83 -0.9798492334336295 2316 84-85 -1.0977974990088875 2316 86-87 -1.0079931472879373 2316 88-89 -1.1799773169005476 2316 90-91 -1.066150145828761 2316 92-93 -1.147234505605546 2316 94-95 -1.171326441316328 2316 96-97 -1.2010489077481168 2316 98-99 -1.1073393041140847 2316 100-101 -0.9005140409258043 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7125.0 3 2610.0 4 2647.0 5 3079.0 6 3520.0 7 4727.0 8 5915.0 9 7556.0 10 9853.0 11 12091.0 12 14868.0 13 17668.0 14 20175.0 15 24041.0 16 27681.0 17 32175.0 18 29599.0 19 26895.0 20 27381.0 21 29944.0 22 32507.0 23 36294.0 24 39686.0 25 44935.0 26 50505.0 27 56987.0 28 64594.0 29 74156.0 30 85435.0 31 99206.0 32 117218.0 33 140023.0 34 171209.0 35 211429.0 36 239333.0 37 224714.0 38 100060.0 39 5380.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.159498424717505 18.454765527274255 14.784838418029578 22.600897629978668 2 15.720459415812218 20.116780474114453 39.73442550428473 24.428334605788603 3 19.29468633963169 24.52676713718021 29.63773913754934 26.54080738563876 4 12.909868819143957 16.46648932324074 36.21083507441954 34.41280678319576 5 13.627465112705478 37.65763091328522 33.492563926539844 15.22234004746946 6 32.32442271954356 35.8373480734481 16.880511247774564 14.957717959233777 7 28.89698321088795 33.24080565668173 19.8539407182342 18.00827041419612 8 27.47126261838355 33.83672106570882 19.160727671216197 19.531288644691433 9 27.26546691616809 16.51342370307935 19.58787552453806 36.633233856214495 10-11 25.432355804300123 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22.042680700589326 36-37 22.822336488676275 25.525502669463418 29.179291328638097 22.47286951322221 38-39 22.18935628849309 26.060899341307397 29.109956483485128 22.639787886714384 40-41 22.526066034032144 25.970109219696592 29.24848354856176 22.255341197709505 42-43 22.238409972436173 25.98349499418858 28.83701199758793 22.94108303578732 44-45 22.144203271185237 26.1957285872452 29.17641705436419 22.483651087205374 46-47 22.912615357547473 26.64647302805909 28.17445306583895 22.266458548554485 48-49 22.48627363522737 26.84083551020612 27.853114047528305 22.81977680703821 50-51 22.56733710743053 26.968817930599315 28.020448692938892 22.443396269031265 52-53 22.97416913634402 26.557394588464376 27.931569430922142 22.536866844269458 54-55 22.321731925329683 26.946721969207942 28.031374562970836 22.70017154249154 56-57 22.481990271180155 27.085966438900062 27.687313067336177 22.744730222583605 58-59 22.49025966229375 26.443028623884707 27.857021091245926 23.209690622575614 60-61 21.968046356986072 26.92402795266155 28.27622559182571 22.831700098526664 62-63 22.161540310427128 26.790250457115324 28.479253228930435 22.568956003527116 64-65 21.93374447831028 26.977727797933497 28.102749789593307 22.985777934162915 66-67 21.863552765214482 26.945316690187187 27.99786793766238 23.193262606935956 68-69 22.483961221629002 26.47758375066808 28.200314023398644 22.83814100430427 70-71 22.297677597220165 26.671785446859285 27.72644598208649 23.30409097383406 72-73 22.17427788045904 26.726335139746777 27.811467218125674 23.287919761668512 74-75 22.142784449419906 27.29562138214274 27.63094194117837 22.930652227258978 76-77 22.311139853871595 26.728777975648438 27.804174621780408 23.15590754869956 78-79 22.340657457009673 26.988707483672457 27.69240059464077 22.978234464677097 80-81 22.694303844114717 27.031575043329205 27.225028945572816 23.049092166983264 82-83 22.50806725210362 26.998982217453605 27.43847598415958 23.054474546283192 84-85 22.2482207319439 27.247930685574516 27.419961466626596 23.08388711585498 86-87 22.639236896431292 27.131892421626087 27.10935365816256 23.11951702378006 88-89 22.391672528504223 27.09234308467252 26.916464177632943 23.599520209190313 90-91 22.28284780808502 27.048473885706592 27.04704696610733 23.621631340101054 92-93 22.58340193433422 26.6103163540506 27.277232219500373 23.529049492114808 94-95 22.274651034097673 26.226526236086134 27.621771543711432 23.877051186104765 96-97 22.5444295121975 25.823921935238232 27.628356218038917 24.00329233452535 98-99 22.297961321777407 26.247937531610248 27.06555362132474 24.388547525287603 100-101 22.729327286632063 25.48928319550691 27.455651216445197 24.325738301415825 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2006.0 1 1295.5 2 858.0 3 1100.0 4 904.5 5 826.0 6 1397.5 7 2929.5 8 4589.0 9 5041.0 10 3995.0 11 2894.5 12 2700.0 13 2779.0 14 2910.5 15 3048.5 16 3109.0 17 3260.5 18 3548.0 19 3848.5 20 4309.5 21 5006.0 22 5739.5 23 6735.5 24 8073.5 25 9837.5 26 12330.0 27 14968.0 28 17078.5 29 19376.5 30 22515.0 31 27339.5 32 34553.0 33 37633.0 34 36933.0 35 38884.0 36 43088.5 37 46973.5 38 49928.5 39 53037.0 40 55524.0 41 58455.5 42 60408.5 43 61528.5 44 63502.5 45 67421.5 46 69900.5 47 71950.5 48 75892.5 49 75605.5 50 73457.5 51 72000.5 52 71761.5 53 74191.5 54 76489.0 55 74259.0 56 69424.0 57 64254.0 58 59851.5 59 53861.0 60 44553.5 61 37340.5 62 32030.5 63 24410.5 64 16674.5 65 12383.0 66 9546.0 67 7408.0 68 5800.5 69 4410.5 70 3345.5 71 2454.0 72 1812.0 73 1369.5 74 1004.5 75 746.5 76 576.0 77 420.0 78 280.0 79 191.0 80 120.0 81 71.5 82 48.0 83 31.5 84 20.5 85 15.5 86 11.0 87 7.5 88 6.0 89 3.0 90 2.5 91 4.0 92 2.5 93 1.5 94 0.5 95 1.0 96 1.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18614306342509893 2 0.046880475232987884 3 0.04654765238650622 4 0.05158754120465704 5 0.058862097706327575 6 0.0657562852405905 7 0.037418797168723594 8 0.034851306638722226 9 0.04255377822872632 10-11 0.04424166552159759 12-13 0.039605918731317344 14-15 0.03563581763400042 16-17 0.032973234862147156 18-19 0.041555309689281346 20-21 0.052181867716231435 22-23 0.04887741231187783 24-25 0.0704395781517967 26-27 0.05417880479512138 28-29 0.046143510358635634 30-31 0.05600933045077051 32-33 0.051682633446508947 34-35 0.051183399176786465 36-37 0.04847327028400724 38-39 0.027148835048718133 40-41 0.025318309393069012 42-43 0.022108946230567306 44-45 0.004730839032132144 46-47 0.011815211050098872 48-49 0.011529934324543165 50-51 0.009580543366579165 52-53 0.01015109681769058 54-55 0.011577480445469118 56-57 0.009081309096856678 58-59 0.011387295961765312 60-61 0.011268430659450433 62-63 0.004112739460094778 64-65 0.00717946425981863 66-67 0.004421789246113461 68-69 0.009176401338708581 70-71 0.02408211024899428 72-73 0.020706335663251748 74-75 0.021728577263159698 76-77 0.01312272937556253 78-79 0.004873477394909997 80-81 0.006466272445929362 82-83 0.005681761450651168 84-85 0.00518252718092868 86-87 0.008439436464356336 88-89 0.03033442515075686 90-91 0.037561435531501444 92-93 0.022917230286308477 94-95 0.03268795813659145 96-97 0.036206371085111835 98-99 0.021942534807326476 100-101 0.12226484996108349 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2103221.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.01145741275267 #Duplication Level Percentage of deduplicated Percentage of total 1 73.63969075033002 34.61909185597417 2 13.261443467770054 12.46879569633398 3 5.0302165667613234 7.094334357156688 4 2.450460495991141 4.607988769956814 5 1.4163390944897005 3.329208251130962 6 0.8967676626710336 2.529501286967583 7 0.61100122680077 2.0106840707018825 8 0.43956043048463755 1.6531501166447815 9 0.32302823845435946 1.3667425447692272 >10 1.742409740116237 14.953478503959166 >50 0.12137192867831509 3.8606665043088606 >100 0.05885422619618171 5.187298763371915 >500 0.004275393020256114 1.4603059100326672 >1k 0.004275393020256112 3.566419726206275 >5k 2.0359014382171956E-4 0.7439592225911136 >10k+ 1.0179507191085978E-4 0.5483744198939645 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11459 0.5448309996904748 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9101 0.43271724654708177 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6445 0.30643474936775544 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3250 0.15452489300934139 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3237 0.153906793437304 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3125 0.1485816278935975 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2883 0.1370754666295173 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2841 0.13507852955062735 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2679 0.12737605796062323 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2531 0.12033923206358248 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2445 0.11625026566395068 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2273 0.10807233286468707 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2213 0.10521956560912998 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2182 0.10374563586042553 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2164 0.10288980568375838 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.754612092595119E-5 2 0.0 0.0 0.0 0.0 4.754612092595119E-5 3 0.0 0.0 0.0 0.0 4.754612092595119E-5 4 0.0 0.0 0.0 0.0 4.754612092595119E-5 5 0.0 0.0 0.0 0.0 4.754612092595119E-5 6 0.0 0.0 0.0 0.0 4.754612092595119E-5 7 0.0 0.0 0.0 0.0 4.754612092595119E-5 8 0.0 0.0 0.0 0.0 4.754612092595119E-5 9 0.0 0.0 0.0 0.0 4.754612092595119E-5 10-11 0.0 0.0 0.0 0.0 4.754612092595119E-5 12-13 0.0 0.0 0.0 2.3773060462975597E-5 3.090497860186828E-4 14-15 0.0 0.0 0.0 4.754612092595119E-5 3.5659590694463395E-4 16-17 0.0 0.0 0.0 4.754612092595119E-5 4.2791508833356075E-4 18-19 0.0 4.754612092595119E-5 0.0 4.754612092595119E-5 4.7546120925951195E-4 20-21 0.0 4.754612092595119E-5 0.0 1.6641142324082917E-4 5.467803906484388E-4 22-23 0.0 4.754612092595119E-5 0.0 5.943265115743899E-4 5.705534511114144E-4 24-25 0.0 4.754612092595119E-5 0.0 0.0013550644463896089 5.705534511114144E-4 26-27 0.0 4.754612092595119E-5 0.0 0.0029716325578719497 5.705534511114144E-4 28-29 0.0 4.754612092595119E-5 0.0 0.006252314901762582 5.705534511114144E-4 30-31 0.0 4.754612092595119E-5 0.0 0.017806022286768725 5.705534511114144E-4 32-33 0.0 4.754612092595119E-5 0.0 0.03725238574548276 5.705534511114144E-4 34-35 0.0 4.754612092595119E-5 0.0 0.06699248438466524 6.180995720373656E-4 36-37 0.0 4.754612092595119E-5 0.0 0.1164166770871915 6.656456929633167E-4 38-39 0.0 4.754612092595119E-5 0.0 0.194844003554548 6.656456929633167E-4 40-41 0.0 4.754612092595119E-5 0.0 0.2767897429704249 6.656456929633167E-4 42-43 0.0 4.754612092595119E-5 0.0 0.360518461921025 6.656456929633167E-4 44-45 0.0 4.754612092595119E-5 0.0 0.45991362771672595 7.607379348152191E-4 46-47 0.0 4.754612092595119E-5 0.0 0.585554252263552 8.796032371300971E-4 48-49 0.0 4.754612092595119E-5 0.0 0.7154264815727875 9.509224185190239E-4 50-51 4.754612092595119E-5 4.754612092595119E-5 0.0 0.8599191430667533 9.509224185190239E-4 52-53 4.754612092595119E-5 4.754612092595119E-5 0.0 0.9989202275937716 9.509224185190239E-4 54-55 4.754612092595119E-5 4.754612092595119E-5 0.0 1.147810905273388 0.0010222415999079507 56-57 4.754612092595119E-5 4.754612092595119E-5 0.0 1.3262514971084827 0.0010697877208339021 58-59 4.754612092595119E-5 4.754612092595119E-5 0.0 1.514771866579879 0.0010935607812968776 60-61 4.754612092595119E-5 4.754612092595119E-5 0.0 1.726518516123603 0.00118865302314878 62-63 4.754612092595119E-5 4.754612092595119E-5 0.0 1.929849502263433 0.0012837452650006822 64-65 4.754612092595119E-5 4.754612092595119E-5 0.0 2.143925911732528 0.0012837452650006822 66-67 4.754612092595119E-5 4.754612092595119E-5 0.0 2.3902861373103446 0.0012837452650006822 68-69 9.509224185190239E-5 4.754612092595119E-5 0.0 2.6695720516293817 0.0012837452650006822 70-71 9.509224185190239E-5 4.754612092595119E-5 0.0 2.962788979379723 0.0012837452650006822 72-73 9.509224185190239E-5 4.754612092595119E-5 0.0 3.2642313860502536 0.0013550644463896089 74-75 9.509224185190239E-5 4.754612092595119E-5 0.0 3.5662205731114325 0.0014263836277785358 76-77 9.509224185190239E-5 4.754612092595119E-5 0.0 3.885825597975676 0.0014263836277785358 78-79 9.509224185190239E-5 4.754612092595119E-5 0.0 4.255045000026151 0.0014977028091674627 80-81 9.509224185190239E-5 4.754612092595119E-5 0.0 4.643924723079506 0.0015452489300934137 82-83 9.509224185190239E-5 4.754612092595119E-5 0.0 5.048209389312868 0.0015690219905563894 84-85 9.509224185190239E-5 4.754612092595119E-5 0.0 5.4488092311744705 0.0015690219905563894 86-87 9.509224185190239E-5 4.754612092595119E-5 0.0 5.871399153964324 0.0016165681114823406 88-89 9.509224185190239E-5 4.754612092595119E-5 0.0 6.315622561775486 0.0016641142324082918 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7870 0.0 38.152542 1 GTATCAA 12260 0.0 34.310783 1 TATCAAC 15215 0.0 27.424791 2 ATCAACG 15550 0.0 26.865173 3 TCAACGC 15700 0.0 26.608496 4 CAACGCA 15915 0.0 26.218552 5 AACGCAG 16425 0.0 25.457483 6 GTATAGG 460 0.0 24.826885 1 GTACATG 10100 0.0 24.49914 1 TACATGG 10050 0.0 24.258955 2 GAGTACT 10070 0.0 22.947836 12-13 ACGCAGA 18210 0.0 22.879427 7 ACATGGG 10410 0.0 22.827082 3 CGCAGAG 18205 0.0 22.807425 8 GTGTAAG 970 0.0 22.566017 1 TAAGGTA 685 0.0 22.20192 4 GTACTTT 10470 0.0 21.709478 14-15 GTGTACG 245 7.374183E-9 21.364632 1 AGTACTT 10520 0.0 21.266378 12-13 AGAGTAC 15365 0.0 21.115294 10-11 >>END_MODULE