FastQCFastQC Report
Thu 26 May 2016
SRR938514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938514_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103221
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110700.5263355586502798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92140.4380899582117143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68300.3247400059242467No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC33180.15775802923230606No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA32780.15585618439526802No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32670.15533317706508254No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30280.14396965416378021No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA28460.1353162601552571No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28400.13503098342970138No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27230.1294680872813651No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA23760.11296958332006003No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA21840.10384072810227742No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21630.10284225956283244No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA21620.10279471344190648No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21550.10246189059542482No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80400.037.675571
GTATCAA122300.032.0777551
AGGTAAG11750.028.3293532
TAAGGTG14700.027.4841125
TATCAAC150300.025.9120272
GTGTAAG8850.025.791371
GTACATG102000.025.641241
ATCAACG152650.025.5015893
TCAACGC153150.025.4493644
GTAAGGT13900.025.3044574
TACATGG101500.025.0647962
CAACGCA155800.024.9859895
AACGCAG160000.024.3412826
TAAGGTA7800.023.7656734
ACATGGG102650.023.7077923
GGTAAGG14750.023.2017423
AAGGTAA13800.023.0872481
GAGTACT98450.022.31236512-13
ACGCAGA176800.021.9208557
CGCAGAG178650.021.5875138
Produced by FastQC (version 0.11.5)