##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938514_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2103221 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.777024858538404 31.0 31.0 34.0 30.0 34.0 2 31.993304555251207 31.0 31.0 34.0 30.0 34.0 3 32.04703357374237 33.0 31.0 34.0 30.0 34.0 4 35.73198346726283 37.0 35.0 37.0 33.0 37.0 5 35.60618736690058 37.0 35.0 37.0 33.0 37.0 6 35.64414628800302 37.0 35.0 37.0 33.0 37.0 7 35.58033606549193 37.0 35.0 37.0 33.0 37.0 8 35.56719907228009 37.0 35.0 37.0 33.0 37.0 9 37.13493113657576 39.0 37.0 39.0 33.0 39.0 10-11 37.047395875183824 39.0 37.0 39.0 33.0 39.0 12-13 36.93872826488514 39.0 37.0 39.0 33.0 39.0 14-15 37.95543739816215 40.0 37.0 41.0 33.0 41.0 16-17 37.88261694800499 40.0 37.0 41.0 33.0 41.0 18-19 38.016479723243535 40.0 37.0 41.0 33.0 41.0 20-21 38.07015026000596 40.0 37.0 41.0 33.0 41.0 22-23 37.97223995956678 40.0 37.0 41.0 33.0 41.0 24-25 37.867209865249535 40.0 37.0 41.0 32.0 41.0 26-27 37.661668697678465 40.0 37.0 41.0 32.0 41.0 28-29 37.535558079726286 39.5 36.5 41.0 32.0 41.0 30-31 37.25983503397883 39.0 36.0 41.0 31.0 41.0 32-33 36.99799545554176 39.0 35.5 41.0 31.0 41.0 34-35 36.819341143893105 39.0 35.0 40.5 30.0 41.0 36-37 36.783922136570524 39.0 35.0 41.0 30.0 41.0 38-39 36.637889218489164 39.0 35.0 41.0 30.0 41.0 40-41 36.437187532836546 39.0 35.0 40.5 30.0 41.0 42-43 36.181773574912 39.0 35.0 40.0 30.0 41.0 44-45 35.8730257067612 38.0 35.0 40.0 29.0 41.0 46-47 35.44160219016452 38.0 34.5 40.0 27.5 41.0 48-49 35.01985407144565 38.0 34.0 40.0 26.0 41.0 50-51 34.6282014586199 38.0 33.5 40.0 24.5 41.0 52-53 34.12173851440243 38.0 33.0 40.0 23.5 41.0 54-55 33.800884928402674 37.0 33.0 40.0 23.0 41.0 56-57 33.44403179694383 37.0 32.5 40.0 22.0 41.0 58-59 32.98624039033463 36.0 32.0 39.0 21.0 41.0 60-61 32.602709843616054 36.0 31.0 39.0 19.5 40.0 62-63 32.139701914349466 35.0 31.0 39.0 18.5 40.0 64-65 31.63664541196574 35.0 30.5 38.0 16.0 40.0 66-67 31.13760560587784 35.0 30.0 38.0 13.0 40.0 68-69 30.59958297297336 34.0 29.0 37.0 8.0 39.5 70-71 30.01952624094187 34.0 29.0 36.5 7.5 39.0 72-73 29.586225603491027 34.0 28.0 36.0 7.0 39.0 74-75 29.097719165033062 34.0 28.0 35.5 2.0 38.0 76-77 27.857389689433493 31.5 26.0 34.5 2.0 36.0 78-79 28.562163462612823 33.0 27.5 35.0 2.0 37.0 80-81 28.31254347498432 33.0 27.0 35.0 2.0 36.5 82-83 27.85916244655222 33.0 26.0 35.0 2.0 36.0 84-85 27.349664871166652 33.0 26.0 35.0 2.0 35.5 86-87 26.817892889049702 32.0 25.0 35.0 2.0 35.0 88-89 26.181380843953157 32.0 24.0 34.0 2.0 35.0 90-91 25.660912238894532 32.0 22.5 34.0 2.0 35.0 92-93 24.95275769878677 31.0 19.5 34.0 2.0 35.0 94-95 24.20090470758898 31.0 14.5 34.0 2.0 35.0 96-97 23.226386337907428 30.5 2.0 34.0 2.0 35.0 98-99 22.015995703732514 30.0 2.0 34.0 2.0 35.0 100-101 18.431486990668123 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21209326752338598 1101 2 0.23097622237176907 1101 3 0.23810456155412396 1101 4 0.14124891909822423 1101 5 0.1321471494203479 1101 6 0.15385747815368944 1101 7 0.20650059498434814 1101 8 0.024994668311720147 1101 9 0.09808479912603474 1101 10-11 0.25062689449173803 1101 12-13 0.2449843086874992 1101 14-15 0.40441087242529505 1101 16-17 0.4221840335841378 1101 18-19 0.3847460293070597 1101 20-21 0.35783786654010186 1101 22-23 0.36447348846813554 1101 24-25 0.35359806811773353 1101 26-27 0.3976039594382641 1101 28-29 0.387498553719098 1101 30-31 0.32578600649287637 1101 32-33 0.34204793096796493 1101 34-35 0.3052921252633425 1101 36-37 0.5993962393648431 1101 38-39 0.5155901075900147 1101 40-41 0.4557035469213204 1101 42-43 0.42214355389126723 1101 44-45 0.47671787903183116 1101 46-47 0.5311134204665322 1101 48-49 0.5162907195348581 1101 50-51 0.5448407048530299 1101 52-53 0.5296780948469078 1101 54-55 0.3969117544139209 1101 56-57 0.25718890808752093 1101 58-59 0.3032548058774083 1101 60-61 0.35678190875088234 1101 62-63 0.31001324662529584 1101 64-65 0.2362106137260831 1101 66-67 0.28433757736900134 1101 68-69 0.24293149544970305 1101 70-71 0.3003454364595015 1101 72-73 0.37054227007112317 1101 74-75 0.46389757927044073 1101 76-77 0.378860902326533 1101 78-79 0.24623645053252474 1101 80-81 0.2697820597067313 1101 82-83 0.3048867254032608 1101 84-85 0.4887211912194651 1101 86-87 0.42629136921220834 1101 88-89 0.2184917992789117 1101 90-91 0.5380817713340278 1101 92-93 0.7127070056301754 1101 94-95 0.7937866799632829 1101 96-97 0.74740737179307 1101 98-99 0.5124958427586641 1101 100-101 0.23463414377713576 1102 1 0.3042856026304186 1102 2 0.3008938216499395 1102 3 0.28076440474188047 1102 4 0.18901533517826152 1102 5 0.16304876599480878 1102 6 0.15345909494349286 1102 7 0.286390425876057 1102 8 0.1801005951771799 1102 9 0.3305707426589777 1102 10-11 0.3324752633816743 1102 12-13 0.340123333660749 1102 14-15 0.49047550848608523 1102 16-17 0.3610493036125817 1102 18-19 0.3565670120430724 1102 20-21 0.3662309663275565 1102 22-23 0.34291961090744394 1102 24-25 0.3624690931684995 1102 26-27 0.31654118608815907 1102 28-29 0.33506620887859384 1102 30-31 0.27877342296699226 1102 32-33 0.22391869545457865 1102 34-35 0.22312639569447157 1102 36-37 0.33852320409393855 1102 38-39 0.3589561926889715 1102 40-41 0.4970088871944185 1102 42-43 0.29763870653663815 1102 44-45 0.2626739825856248 1102 46-47 0.26640984987626126 1102 48-49 0.49673346863742296 1102 50-51 0.36663528124247335 1102 52-53 0.3918434260325583 1102 54-55 0.40290607583811067 1102 56-57 0.32772817359808215 1102 58-59 0.3126544962152096 1102 60-61 0.21521185898528472 1102 62-63 0.0771535496549518 1102 64-65 0.21896465839237678 1102 66-67 0.32587521812209275 1102 68-69 0.34352917793613 1102 70-71 0.1519549584642519 1102 72-73 -0.08657904515336057 1102 74-75 -0.22200592693249277 1102 76-77 -0.13583849600714615 1102 78-79 -0.11832712066070528 1102 80-81 -0.002018115581879698 1102 82-83 0.13836281272024564 1102 84-85 -0.08359572477766974 1102 86-87 -0.20375208147548562 1102 88-89 -0.239052528928557 1102 90-91 -0.27505698769150655 1102 92-93 -0.23445836324037828 1102 94-95 -0.1340318004954817 1102 96-97 -0.03495493835579566 1102 98-99 -0.5410963894147613 1102 100-101 -0.6907886538635744 1103 1 0.2958569475213757 1103 2 0.2751024615816924 1103 3 0.30653386952317874 1103 4 0.19558830032045194 1103 5 0.1588286426290324 1103 6 0.168007611550685 1103 7 0.32675683448744763 1103 8 0.2533825750469063 1103 9 0.33872763861824495 1103 10-11 0.3205629393231817 1103 12-13 0.39185764646041576 1103 14-15 0.4413062929504221 1103 16-17 0.5655049172292834 1103 18-19 0.48941267293813695 1103 20-21 0.5122498622177858 1103 22-23 0.43144998379190724 1103 24-25 0.4811503065486349 1103 26-27 0.5322639855731879 1103 28-29 0.5130702551276229 1103 30-31 0.5175308370975387 1103 32-33 0.6317947907985086 1103 34-35 0.7041308175120733 1103 36-37 0.63595921447385 1103 38-39 0.7098212110784416 1103 40-41 0.8857067719036209 1103 42-43 0.8771144766969172 1103 44-45 0.8770164516630388 1103 46-47 0.7385153830986226 1103 48-49 0.9166107571456905 1103 50-51 0.9977570102797912 1103 52-53 0.9133295287075498 1103 54-55 1.0099060248349687 1103 56-57 1.0432325353968324 1103 58-59 0.8583852501291886 1103 60-61 0.9097611432154764 1103 62-63 0.7332285644535901 1103 64-65 0.7054094150378525 1103 66-67 0.8851941871168236 1103 68-69 0.8729433064238563 1103 70-71 0.7368504561235198 1103 72-73 0.6919353158365489 1103 74-75 0.7730841193670237 1103 76-77 0.8570448917552937 1103 78-79 0.9339589533700909 1103 80-81 0.7983870245041693 1103 82-83 0.8984106886970977 1103 84-85 0.6962914707039722 1103 86-87 0.7151285850891718 1103 88-89 0.6983709985618738 1103 90-91 0.5902168038560909 1103 92-93 0.7320660613386067 1103 94-95 0.7651594648820925 1103 96-97 0.8058661967267895 1103 98-99 0.7302837143122254 1103 100-101 0.4402139390535851 1104 1 0.24301533585623147 1104 2 0.23902481855217772 1104 3 0.2110650414340327 1104 4 0.1397037818690876 1104 5 0.10959735008223248 1104 6 0.050367608883945536 1104 7 0.08502336548124845 1104 8 0.2018850395921561 1104 9 0.31682532794587814 1104 10-11 0.3046916961792121 1104 12-13 0.2657708158281338 1104 14-15 0.3661296335491713 1104 16-17 0.4548687584647766 1104 18-19 0.3686324371846439 1104 20-21 0.28051934706481063 1104 22-23 0.26924426811025626 1104 24-25 0.19172403049134346 1104 26-27 0.2847129586103918 1104 28-29 0.39451587186735537 1104 30-31 0.4566516545606021 1104 32-33 0.38017674105211086 1104 34-35 0.20633087853565968 1104 36-37 0.40855984135254886 1104 38-39 0.4243712010294942 1104 40-41 0.4554080969525458 1104 42-43 0.431154905068901 1104 44-45 0.2810813089364572 1104 46-47 0.3155981780958541 1104 48-49 0.28317700740178253 1104 50-51 0.3479174526862252 1104 52-53 0.4223992449965195 1104 54-55 0.3663443785995284 1104 56-57 0.4471285315388158 1104 58-59 0.34845407442261234 1104 60-61 0.2427928866750264 1104 62-63 0.2985617634766271 1104 64-65 0.4044200445297683 1104 66-67 0.17527814564997612 1104 68-69 -0.09629744623935821 1104 70-71 -0.31929781660229395 1104 72-73 -0.3717315352634536 1104 74-75 -0.14913148466676063 1104 76-77 -0.23150707781271862 1104 78-79 -0.22275719398776772 1104 80-81 -0.21143271258829444 1104 82-83 -0.21616828015994471 1104 84-85 -0.03550303649160824 1104 86-87 0.13530556810037808 1104 88-89 0.1448369102684275 1104 90-91 0.14086772215406995 1104 92-93 0.12374821505284217 1104 94-95 0.09097896449838316 1104 96-97 0.0956855493803701 1104 98-99 -0.12003050364904766 1104 100-101 -0.1776165798464575 1105 1 0.27033142199585214 1105 2 0.30758230985101065 1105 3 0.12719663755436983 1105 4 0.19161063844804715 1105 5 0.14310239006067604 1105 6 0.2148175794132854 1105 7 0.13943793664223136 1105 8 0.2538303912841968 1105 9 0.2411563831522514 1105 10-11 0.2761501054366633 1105 12-13 0.3542542535938722 1105 14-15 0.3571563535373272 1105 16-17 0.3009508895173596 1105 18-19 0.3560424606205643 1105 20-21 0.3945795603369575 1105 22-23 0.4312655888706729 1105 24-25 0.37912723084134825 1105 26-27 0.29071980981847645 1105 28-29 0.3116319747419709 1105 30-31 0.38762461508606094 1105 32-33 0.4451476173092317 1105 34-35 0.37229898568023856 1105 36-37 0.3469201754348106 1105 38-39 0.24211666442652557 1105 40-41 0.28501397384240335 1105 42-43 0.27411674212023485 1105 44-45 0.27440067799463463 1105 46-47 0.10073286373715717 1105 48-49 -0.007668785554905355 1105 50-51 0.010045614673664716 1105 52-53 -0.1402635380434205 1105 54-55 -0.06358852234378531 1105 56-57 0.07427673749366903 1105 58-59 0.01622730270808148 1105 60-61 -0.09757514043659654 1105 62-63 0.06545552825949841 1105 64-65 -0.04802823313663751 1105 66-67 0.06893055506371581 1105 68-69 -0.09536710925498326 1105 70-71 -0.02385762037403083 1105 72-73 0.09237522943436005 1105 74-75 -0.006880055333990498 1105 76-77 -0.021978868847416067 1105 78-79 0.024852478474141293 1105 80-81 0.16836226290045886 1105 82-83 0.25464850546122975 1105 84-85 0.18852054188040768 1105 86-87 0.39400482975489126 1105 88-89 0.16610188734539477 1105 90-91 0.20576656414269223 1105 92-93 0.18688930563553185 1105 94-95 0.13134143632722228 1105 96-97 0.00615859438761035 1105 98-99 -0.1154435919413963 1105 100-101 -0.3908803130933016 1106 1 0.14993121409564125 1106 2 0.11186614834537423 1106 3 0.148498430237737 1106 4 0.13338763811978538 1106 5 0.09588829843868751 1106 6 0.04171352900659997 1106 7 0.18329895352956527 1106 8 0.08799555340988974 1106 9 0.09930985545045701 1106 10-11 0.051197179332419296 1106 12-13 0.10181062828840481 1106 14-15 0.22935756537669505 1106 16-17 0.01015172750108917 1106 18-19 0.2582524261528931 1106 20-21 0.21807684304476282 1106 22-23 0.23468591202782818 1106 24-25 0.2688076754560029 1106 26-27 0.13554852010771867 1106 28-29 0.23582089037825682 1106 30-31 0.285199226640934 1106 32-33 0.2147647800184984 1106 34-35 0.16237391638016874 1106 36-37 -0.1565469936573649 1106 38-39 0.07485547611270249 1106 40-41 0.4527887577356111 1106 42-43 0.5608474885549342 1106 44-45 0.506524565546151 1106 46-47 0.22062802458626152 1106 48-49 0.15300821229314465 1106 50-51 0.26336540713818835 1106 52-53 0.18647021747323578 1106 54-55 -0.20655923850529234 1106 56-57 0.17367788459218758 1106 58-59 0.23540096776991248 1106 60-61 -0.17232575137141737 1106 62-63 0.021955031305054717 1106 64-65 -0.34295337082493305 1106 66-67 -0.1259154802428455 1106 68-69 -0.7001516635461122 1106 70-71 -0.3859637041906403 1106 72-73 -0.12771871319247552 1106 74-75 -0.08214562961272165 1106 76-77 0.03810816656856986 1106 78-79 0.02885432951546818 1106 80-81 -0.8265749567078124 1106 82-83 -0.20698364794724 1106 84-85 -0.30539290598040836 1106 86-87 -0.021247454411870592 1106 88-89 0.06090107671695577 1106 90-91 -0.21929270565341596 1106 92-93 -0.4401544765069332 1106 94-95 -0.2732142453666171 1106 96-97 -0.2632057598451638 1106 98-99 -0.36764979612128457 1106 100-101 -0.5021014532618082 1107 1 0.22712727752588435 1107 2 0.28468573382319207 1107 3 0.26966879468341887 1107 4 0.2591839936629796 1107 5 0.2053045941682754 1107 6 0.24529104326449414 1107 7 0.1849142685942482 1107 8 0.26537331805030817 1107 9 0.342004423540601 1107 10-11 0.3898078171123629 1107 12-13 0.3442309057957047 1107 14-15 0.3686393674353994 1107 16-17 0.4903615098073928 1107 18-19 0.4412990077358714 1107 20-21 0.45548468836400247 1107 22-23 0.4312535848613308 1107 24-25 0.47010218790558156 1107 26-27 0.5403518654965112 1107 28-29 0.5801090389385593 1107 30-31 0.37482008837413616 1107 32-33 0.4052511133054608 1107 34-35 0.3948927820082133 1107 36-37 0.41746495167578956 1107 38-39 0.47678550810602616 1107 40-41 0.5437115216805282 1107 42-43 0.5177456141634948 1107 44-45 0.6765999411199459 1107 46-47 0.5815609603214185 1107 48-49 0.6236534689352382 1107 50-51 0.6407553431515467 1107 52-53 0.6113976239275374 1107 54-55 0.44770639301508197 1107 56-57 0.5553330174340303 1107 58-59 0.5267145197242797 1107 60-61 0.42534951639878926 1107 62-63 0.6489428773198043 1107 64-65 0.5219078755236559 1107 66-67 0.599980854435131 1107 68-69 0.6376026312097629 1107 70-71 0.4681936890490306 1107 72-73 0.6809894694476668 1107 74-75 0.6424448906265319 1107 76-77 0.7164144330868609 1107 78-79 0.8739692620512862 1107 80-81 0.8460038094627258 1107 82-83 0.7892493121306607 1107 84-85 0.9349743217285926 1107 86-87 1.0138004486344556 1107 88-89 0.8848339081260512 1107 90-91 0.9091430990482081 1107 92-93 0.7643797946017052 1107 94-95 0.6315208533953829 1107 96-97 0.9102728185384734 1107 98-99 0.8410423029189147 1107 100-101 0.3205373003896028 1108 1 0.21855734970194263 1108 2 0.18819818384293896 1108 3 0.22825723447500224 1108 4 0.1601897006263613 1108 5 0.1713151373316748 1108 6 0.15542389240061283 1108 7 0.2472826955057812 1108 8 0.19742305875907817 1108 9 0.1259060619115786 1108 10-11 0.23178127119814462 1108 12-13 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-0.29583971992593305 2316 70-71 -0.4228115373663677 2316 72-73 -0.37429167843198385 2316 74-75 -0.07559563287355786 2316 76-77 -0.10368003410316362 2316 78-79 -0.1559585393663916 2316 80-81 -0.1848140655218451 2316 82-83 -0.09049014515922593 2316 84-85 -0.11168713104317973 2316 86-87 -0.03892718751259494 2316 88-89 0.24334708953203332 2316 90-91 0.18445888400321309 2316 92-93 0.21926372954517603 2316 94-95 0.22188176581804342 2316 96-97 0.46900761283556847 2316 98-99 0.5019531158035875 2316 100-101 0.6855546591717498 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 45.0 5 86.0 6 204.0 7 495.0 8 1102.0 9 1946.0 10 3265.0 11 5081.0 12 7074.0 13 9624.0 14 12549.0 15 16642.0 16 21148.0 17 26086.0 18 24332.0 19 19702.0 20 18816.0 21 19750.0 22 20876.0 23 23499.0 24 27603.0 25 32080.0 26 38052.0 27 45793.0 28 54576.0 29 65099.0 30 77421.0 31 92741.0 32 111553.0 33 137637.0 34 176678.0 35 232129.0 36 299021.0 37 324312.0 38 149621.0 39 6573.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.92110630892778 19.298453316597012 15.337175079996769 22.44326529447844 2 16.1375534270621 20.896409178156805 38.5449762506749 24.421061144106197 3 19.396234883762947 24.64161787598148 30.066917015103638 25.895230225151934 4 13.400446267891011 17.14693795849319 35.84968008592535 33.60293568769045 5 13.686908511720668 37.84815927491216 33.48720098716411 14.977731226203058 6 32.36535770610887 36.23523158051389 16.55689059780213 14.84252011557511 7 28.93666428777575 33.27134904035287 19.779186305195697 18.012800366675684 8 27.53452918166945 34.20149380402725 18.96595745287823 19.298019561425072 9 26.995451262611013 16.46217872491764 19.557621381680764 36.984748630790584 10-11 25.43646150357 26.599772444265245 26.392209853363006 21.57155619880174 12-13 26.699120063940025 23.993912194676643 27.007290246721578 22.29967749466176 14-15 23.051999766073088 25.269265569333893 25.681918352850225 25.996816311742798 16-17 22.09268545721063 28.50651453175867 26.7042074988791 22.6965925121516 18-19 22.327396883161587 26.40573672476644 29.549034552241537 21.71783183983043 20-21 22.80932436486703 25.386347892114046 29.905250090218765 21.89907765280016 22-23 22.54984140991365 25.400278905545353 30.06633634791589 21.98354333662511 24-25 22.51492712595651 25.826970860842767 29.77087967105645 21.887222342144277 26-27 21.79145824878337 26.08494391350833 29.76931323201841 22.35428460568989 28-29 21.976625376030384 26.601888246646453 29.540975484744592 21.880510892578574 30-31 21.987085522634093 26.078310363009876 29.37175884036913 22.5628452739869 32-33 21.740939254600445 26.134937793032687 29.356924450640232 22.76719850172664 34-35 22.68987161198288 26.199476937708038 29.09676652401331 22.013884926295766 36-37 22.815035323210285 25.528445543394046 29.23214660564596 22.424372527749707 38-39 22.154519141045224 26.061776750212477 29.256836512115825 22.526867596626467 40-41 22.48811642550828 25.951149557822145 29.35144236187024 22.209291654799337 42-43 22.308106691999335 25.994038644683158 28.879100690229663 22.818753973087844 44-45 22.24804240733618 26.155477717272696 29.202447103751815 22.39403277163931 46-47 22.92987755447478 26.6359550422899 28.196490050261 22.23767735297432 48-49 22.502913388559737 26.8248557807287 27.875767691554998 22.79646313915656 50-51 22.645171382370183 26.93948947828117 28.023113120304522 22.392226019044124 52-53 23.1646127534862 26.497999972423248 27.893478621599915 22.44390865249063 54-55 22.413098766130616 26.944386728736543 27.959116041538195 22.68339846359465 56-57 22.540142001244757 26.99737688050852 27.715228214248526 22.7472529039982 58-59 22.5160361179353 26.48452064714074 27.79061258897662 23.208830645947334 60-61 21.992719737963817 26.9715117907248 28.190974700233596 22.84479377107779 62-63 22.21357146966486 26.920661214394492 28.342337776201358 22.523429539739286 64-65 21.930291681188045 27.0509375857316 27.99408621347923 23.024684519601127 66-67 21.88520459105562 27.030125236542062 27.874021738096822 23.210648434305494 68-69 22.531103177703134 26.548050674174583 28.031432081044066 22.889414067078214 70-71 22.365637410301122 26.781181689482896 27.57265950858625 23.28052139162973 72-73 22.186470180737068 26.9272701252032 27.62531849957755 23.260941194482175 74-75 22.205702586651615 27.457456919648482 27.340802511956664 22.99603798174324 76-77 22.285984211835085 26.83396086288602 27.803022126538295 23.0770327987406 78-79 22.289241121118515 27.12732042899914 27.668537923499244 22.914900526383107 80-81 22.604767464334593 27.198258267724775 27.203012941985605 22.993961325955027 82-83 22.44063180581868 27.20920098544629 27.392658696149862 22.95750851258517 84-85 22.237266005442866 27.48675123465346 27.28540756461648 22.990575195287192 86-87 22.604516238851332 27.416903848768953 26.954030257240753 23.02454965513897 88-89 22.24611155197015 27.49816115027598 26.68497829753183 23.57074900022204 90-91 22.125256905391062 27.448752649290416 26.988563623979662 23.437426821338867 92-93 22.40981189866972 27.080619712376585 27.131518306733803 23.378050082219893 94-95 22.07090376313999 26.69924216093727 27.467042154946448 23.762811920976297 96-97 22.369712930785685 26.318418273685936 27.434373277938935 23.877495517589452 98-99 22.022000081303926 26.877817555419202 26.920228725532453 24.17995363774442 100-101 22.162720893334555 26.400150055557642 27.395956012230766 24.041173038877037 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1982.0 1 1278.0 2 823.5 3 1082.5 4 892.0 5 826.0 6 1450.5 7 3131.0 8 4702.5 9 4941.5 10 3904.5 11 2789.0 12 2587.0 13 2673.5 14 2857.5 15 2976.5 16 3068.0 17 3224.5 18 3394.0 19 3708.5 20 4117.0 21 4686.0 22 5495.5 23 6407.5 24 7614.0 25 9383.0 26 11944.0 27 14715.0 28 16886.5 29 19388.0 30 22688.5 31 27704.5 32 35605.5 33 38330.5 34 36867.5 35 38884.0 36 43264.5 37 47501.0 38 50692.0 39 53336.0 40 55530.0 41 58849.5 42 60931.5 43 62390.0 44 64487.5 45 68485.5 46 70585.5 47 73191.5 48 77581.0 49 76268.0 50 73746.5 51 72444.0 52 72024.0 53 74744.0 54 77574.0 55 74914.0 56 70076.0 57 64482.0 58 59452.5 59 53409.0 60 43671.0 61 36512.5 62 31267.0 63 22861.0 64 15009.0 65 11049.5 66 8421.5 67 6667.0 68 5187.0 69 3905.5 70 3012.0 71 2093.0 72 1471.5 73 1140.5 74 860.0 75 658.5 76 480.0 77 328.5 78 240.0 79 160.0 80 104.5 81 58.5 82 36.5 83 22.0 84 11.0 85 7.0 86 4.0 87 2.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.230073301854631E-4 2 0.05020870369780447 3 9.033762975930727E-4 4 0.0 5 0.08786523147115781 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.426383627778536E-4 26-27 0.008296798101578483 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010507692724635214 36-37 2.37730604629756E-5 38-39 0.003970101097316925 40-41 0.00513498106000273 42-43 3.803689674076096E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.75461209259512E-5 68-69 9.271493580560483E-4 70-71 1.664114232408292E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001307518325463658 82-83 5.467803906484388E-4 84-85 0.004778385153058095 86-87 0.002615036650927316 88-89 1.901844837038048E-4 90-91 0.003019178678797901 92-93 9.271493580560483E-4 94-95 1.901844837038048E-4 96-97 0.0 98-99 7.13191813889268E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2103221.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.06963283029116 #Duplication Level Percentage of deduplicated Percentage of total 1 70.83662565896728 31.217440837274697 2 14.553017657383668 12.826922894672846 3 5.610185530051582 7.4171644925755675 4 2.7085874251952884 4.774658132684004 5 1.5815415188791628 3.484897702143285 6 0.9850932747956289 2.604761935429949 7 0.6848060208623662 2.112540492956403 8 0.4887099186931968 1.7229813341864506 9 0.35161763567951837 1.394609409094631 >10 1.982473865011763 16.020008954416983 >50 0.1394656186258496 4.191278798840234 >100 0.06795468904367392 5.692163183523102 >500 0.004852754418463467 1.5273342067872444 >1k 0.0047449154313865 3.724665228786591 >5k 2.156779741539318E-4 0.762478997252304 >10k+ 1.078389870769659E-4 0.5260933993756547 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11070 0.5263355586502798 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9214 0.4380899582117143 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6830 0.3247400059242467 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3318 0.15775802923230606 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3278 0.15585618439526802 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3267 0.15533317706508254 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3028 0.14396965416378021 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2846 0.1353162601552571 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2840 0.13503098342970138 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2723 0.1294680872813651 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2376 0.11296958332006003 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2184 0.10384072810227742 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2163 0.10284225956283244 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2162 0.10279471344190648 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2155 0.10246189059542482 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.509224185190239E-5 2 0.0 0.0 0.0 0.0 9.509224185190239E-5 3 0.0 0.0 0.0 0.0 9.509224185190239E-5 4 0.0 0.0 0.0 0.0 9.509224185190239E-5 5 0.0 0.0 0.0 0.0 9.509224185190239E-5 6 0.0 0.0 0.0 0.0 9.509224185190239E-5 7 0.0 0.0 0.0 4.754612092595119E-5 9.509224185190239E-5 8 0.0 0.0 0.0 4.754612092595119E-5 9.509224185190239E-5 9 0.0 0.0 0.0 4.754612092595119E-5 9.509224185190239E-5 10-11 0.0 0.0 0.0 4.754612092595119E-5 9.509224185190239E-5 12-13 0.0 0.0 0.0 7.131918138892679E-5 1.664114232408292E-4 14-15 0.0 0.0 0.0 9.509224185190239E-5 2.1395754416678037E-4 16-17 0.0 0.0 0.0 9.509224185190239E-5 2.3773060462975597E-4 18-19 0.0 0.0 0.0 9.509224185190239E-5 2.3773060462975597E-4 20-21 0.0 0.0 0.0 2.139575441667804E-4 2.3773060462975597E-4 22-23 0.0 0.0 0.0 6.894187534262923E-4 2.3773060462975597E-4 24-25 0.0 0.0 0.0 0.0014501566882415113 2.3773060462975597E-4 26-27 0.0 0.0 0.0 0.0031380439811127788 3.090497860186828E-4 28-29 0.0 0.0 0.0 0.006442499385466387 3.3282284648165835E-4 30-31 0.0 0.0 0.0 0.01809129901232443 3.3282284648165835E-4 32-33 0.0 0.0 0.0 0.037775393075668226 3.8036896740760955E-4 34-35 0.0 0.0 0.0 0.06761058395670261 3.8036896740760955E-4 36-37 0.0 0.0 0.0 0.11800947213821086 3.8036896740760955E-4 38-39 0.0 0.0 0.0 0.19753035938686425 4.2791508833356075E-4 40-41 0.0 0.0 0.0 0.28018929061663045 4.2791508833356075E-4 42-43 0.0 0.0 0.0 0.3647262936229716 4.2791508833356075E-4 44-45 0.0 0.0 0.0 0.46464446674885806 4.75461209259512E-4 46-47 0.0 0.0 0.0 0.5910933753514253 5.230073301854632E-4 48-49 0.0 0.0 0.0 0.7217025695350132 5.230073301854632E-4 50-51 0.0 0.0 0.0 0.867027288145183 6.180995720373655E-4 52-53 0.0 0.0 0.0 1.0067653375465535 6.180995720373655E-4 54-55 0.0 0.0 0.0 1.1568208951888557 6.180995720373655E-4 56-57 0.0 0.0 0.0 1.3357607212936728 6.180995720373655E-4 58-59 0.0 0.0 0.0 1.5241859985232176 6.180995720373655E-4 60-61 0.0 0.0 0.0 1.7363605631552748 6.180995720373655E-4 62-63 0.0 0.0 0.0 1.9414745288298283 6.180995720373655E-4 64-65 0.0 0.0 0.0 2.1565256337779055 7.131918138892679E-4 66-67 0.0 0.0 0.0 2.403694143411463 7.369648743522435E-4 68-69 0.0 0.0 0.0 2.685357363776798 7.607379348152191E-4 70-71 0.0 0.0 0.0 2.981355739601307 8.558301766671215E-4 72-73 0.0 0.0 0.0 3.286887112671469 0.001117333841759853 74-75 0.0 0.0 0.0 3.594011280792651 0.001307518325463658 76-77 0.0 0.0 0.0 3.919654663014491 0.0013312913859266334 78-79 0.0 2.3773060462975597E-5 0.0 4.295982210143395 0.0013788375068525846 80-81 0.0 4.754612092595119E-5 0.0 4.693230050479716 0.0013788375068525846 82-83 0.0 4.754612092595119E-5 0.0 5.1071665792610474 0.001473929748704487 84-85 0.0 4.754612092595119E-5 0.0 5.518060156303118 0.0015214758696304382 86-87 0.0 4.754612092595119E-5 0.0 5.952869432170941 0.001640341171945316 88-89 0.0 4.754612092595119E-5 0.0 6.4158735577478545 0.00178297953472317 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8040 0.0 37.67557 1 GTATCAA 12230 0.0 32.077755 1 AGGTAAG 1175 0.0 28.329353 2 TAAGGTG 1470 0.0 27.484112 5 TATCAAC 15030 0.0 25.912027 2 GTGTAAG 885 0.0 25.79137 1 GTACATG 10200 0.0 25.64124 1 ATCAACG 15265 0.0 25.501589 3 TCAACGC 15315 0.0 25.449364 4 GTAAGGT 1390 0.0 25.304457 4 TACATGG 10150 0.0 25.064796 2 CAACGCA 15580 0.0 24.985989 5 AACGCAG 16000 0.0 24.341282 6 TAAGGTA 780 0.0 23.765673 4 ACATGGG 10265 0.0 23.707792 3 GGTAAGG 1475 0.0 23.201742 3 AAGGTAA 1380 0.0 23.087248 1 GAGTACT 9845 0.0 22.312365 12-13 ACGCAGA 17680 0.0 21.920855 7 CGCAGAG 17865 0.0 21.587513 8 >>END_MODULE