FastQCFastQC Report
Thu 26 May 2016
SRR938513_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938513_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104846
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117270.557142897865212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89410.42478167048800713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66650.3166502442458973No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33310.1582538580019631No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32830.15597340613042474No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA31820.15117495531739614No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA28510.13544933928657962No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27940.13274130268912784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27260.12951066253778187No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24910.11834595025004203No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24290.11540036658263834No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA23700.1125973111572058No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA23010.10931916159186944No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22050.10475825784879272No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21520.10224025890730248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73500.036.8911131
GTATCAA117350.036.66921
AGGTAAG12350.033.093232
TAAGGTG14850.029.7621445
TATCAAC145450.029.5040552
TCAACGC148800.028.9036944
ATCAACG148950.028.8745863
GGTAAGG15450.028.606333
GTAAGGT15250.028.3582364
CAACGCA152000.028.2639315
AACGCAG156450.027.545896
GTACATG105850.026.2016661
AAGGTAA14800.026.0807511
TACATGG105900.025.7138062
ACGCAGA174100.024.6394777
CGCAGAG174850.024.3164358
ACATGGG107150.024.216323
GAGTACT94850.023.56205612-13
GCAGAGT183050.022.5513159
TAAGGTA7200.022.4416144
Produced by FastQC (version 0.11.5)