##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938513_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2104846 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.039192891071366 31.0 31.0 34.0 28.0 34.0 2 31.17603615656442 31.0 31.0 34.0 27.0 34.0 3 31.2139933277779 31.0 31.0 34.0 27.0 34.0 4 34.90104976801153 37.0 35.0 37.0 32.0 37.0 5 34.90980195225684 37.0 35.0 37.0 32.0 37.0 6 34.92243945637828 37.0 35.0 37.0 32.0 37.0 7 34.83551813291804 37.0 35.0 37.0 32.0 37.0 8 34.79144554993572 37.0 35.0 37.0 32.0 37.0 9 36.2556666853537 39.0 35.0 39.0 32.0 39.0 10-11 36.10882862689242 39.0 35.0 39.0 31.0 39.0 12-13 35.9391342644545 38.0 35.0 39.0 30.0 39.0 14-15 36.875824169559195 39.0 36.0 41.0 30.5 41.0 16-17 36.7054775028672 39.0 36.0 41.0 30.0 41.0 18-19 36.606681201380056 39.0 36.0 40.0 30.0 41.0 20-21 36.59308258181359 39.0 36.0 40.5 30.0 41.0 22-23 36.679630243732795 39.0 36.0 41.0 30.0 41.0 24-25 36.57632363602848 39.0 36.0 41.0 30.0 41.0 26-27 36.36752213701145 39.0 35.5 41.0 29.5 41.0 28-29 36.167008655265036 39.0 35.0 40.5 29.0 41.0 30-31 35.85509866280003 38.5 35.0 40.0 27.5 41.0 32-33 35.50089769037734 38.0 34.5 40.0 27.0 41.0 34-35 35.211876070743415 38.0 34.0 40.0 26.0 41.0 36-37 34.89662189062763 38.0 34.0 40.0 25.0 41.0 38-39 34.53601759938732 38.0 33.0 40.0 24.0 41.0 40-41 34.36571535399739 38.0 33.0 40.0 24.0 41.0 42-43 34.08335669212855 38.0 33.0 40.0 23.0 41.0 44-45 33.712012660308645 38.0 33.0 40.0 21.5 41.0 46-47 33.236693325782504 37.0 32.0 40.0 18.5 41.0 48-49 32.76685990328984 37.0 31.0 40.0 15.0 41.0 50-51 31.942173204120397 36.0 30.5 39.0 13.0 40.5 52-53 31.937390193866918 36.0 30.5 39.0 10.5 40.0 54-55 31.98429053716994 36.0 31.0 39.0 8.5 41.0 56-57 31.62015178307582 36.0 30.0 39.0 7.0 41.0 58-59 31.153913160392733 35.5 29.5 39.0 2.0 40.5 60-61 30.628808473399005 35.0 28.5 39.0 2.0 40.0 62-63 30.023974438034898 35.0 28.0 38.0 2.0 40.0 64-65 29.501874483928994 34.0 27.5 38.0 2.0 40.0 66-67 28.92060963129844 34.0 26.0 37.5 2.0 40.0 68-69 28.27011382305404 34.0 26.0 36.5 2.0 39.0 70-71 27.600854409301203 33.0 24.5 36.0 2.0 39.0 72-73 26.95336713469774 33.0 23.5 35.5 2.0 38.5 74-75 26.24255503728064 32.0 21.0 35.0 2.0 38.0 76-77 25.504839308909062 31.5 19.0 35.0 2.0 37.0 78-79 24.70352937934652 31.0 14.0 35.0 2.0 36.5 80-81 23.932451352735544 31.0 7.0 34.5 2.0 36.0 82-83 23.189557098239014 30.0 2.0 34.0 2.0 35.5 84-85 22.44299796754727 29.5 2.0 34.0 2.0 35.0 86-87 21.729424622989043 29.0 2.0 34.0 2.0 35.0 88-89 20.939439749986462 28.5 2.0 34.0 2.0 35.0 90-91 20.09806821971774 27.0 2.0 34.0 2.0 35.0 92-93 19.227241565416186 25.5 2.0 33.0 2.0 35.0 94-95 18.324016816432177 24.5 2.0 33.0 2.0 35.0 96-97 17.259617093127005 21.0 2.0 32.5 2.0 35.0 98-99 16.035797630800545 4.5 2.0 32.0 2.0 34.0 100-101 13.391774030024049 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6648688018689093 1101 2 0.6216343630482832 1101 3 0.5118262954415513 1101 4 0.42848235396574097 1101 5 0.6507526674925757 1101 6 0.49325004965412944 1101 7 0.4931608001124985 1101 8 0.39668105614591553 1101 9 0.6975217638243763 1101 10-11 0.7706766512100174 1101 12-13 0.8551349350768263 1101 14-15 0.9388782414902508 1101 16-17 1.0565577363943248 1101 18-19 1.1651660320196342 1101 20-21 0.9909583106736974 1101 22-23 0.9637533973930701 1101 24-25 1.0140131876269933 1101 26-27 1.1535559761884642 1101 28-29 1.1628590921410762 1101 30-31 1.1842487586619157 1101 32-33 1.4313060839104352 1101 34-35 1.3064765420779665 1101 36-37 1.357629654230223 1101 38-39 1.5850945278500745 1101 40-41 1.670230420101312 1101 42-43 1.7816821744832723 1101 44-45 1.8860572025002327 1101 46-47 1.991926638832986 1101 48-49 2.0591075713521363 1101 50-51 1.9231826718712206 1101 52-53 2.0507365664712047 1101 54-55 2.3058197184091256 1101 56-57 2.2718932938635312 1101 58-59 2.287176180957715 1101 60-61 2.513933551551819 1101 62-63 2.481565235610116 1101 64-65 2.517304497774589 1101 66-67 2.660126094296146 1101 68-69 2.6882119607215316 1101 70-71 3.1363691403916825 1101 72-73 3.4317844034384173 1101 74-75 3.6273689780388985 1101 76-77 3.791972502056719 1101 78-79 4.1498734200617875 1101 80-81 4.687971183563281 1101 82-83 4.568381997630421 1101 84-85 4.54040355584149 1101 86-87 4.501941378999877 1101 88-89 4.892602120990976 1101 90-91 5.1757506116093985 1101 92-93 5.272274867296186 1101 94-95 5.7060277156532315 1101 96-97 5.932155548532883 1101 98-99 5.971110697240185 1101 100-101 5.971894611613406 1102 1 0.657678572700874 1102 2 0.6358057903689662 1102 3 0.5430299201050239 1102 4 0.5449498392460157 1102 5 0.47422761660282475 1102 6 0.47988519750800407 1102 7 0.5835946215496648 1102 8 0.6659786681580826 1102 9 0.7472569182446733 1102 10-11 0.8223220864541858 1102 12-13 0.7788298592346834 1102 14-15 1.0104341545508646 1102 16-17 1.0307276315504907 1102 18-19 1.200836463433383 1102 20-21 1.190052692007555 1102 22-23 1.0345700410772665 1102 24-25 1.0410931009502633 1102 26-27 1.0497759074599387 1102 28-29 1.108924712558263 1102 30-31 1.1990045973031087 1102 32-33 1.0512466578154829 1102 34-35 1.1962783456544699 1102 36-37 1.3960410185132872 1102 38-39 1.4207605816509385 1102 40-41 1.5933710290272671 1102 42-43 1.7562963973475476 1102 44-45 1.7674238932437731 1102 46-47 1.9992399883634917 1102 48-49 1.7789201000802208 1102 50-51 2.0027397668873057 1102 52-53 2.1853820892676303 1102 54-55 2.1604446664249792 1102 56-57 2.0966776964288165 1102 58-59 2.2497667428243346 1102 60-61 2.298266622228372 1102 62-63 2.5801047545104545 1102 64-65 2.6002597147947455 1102 66-67 2.7517511736403826 1102 68-69 2.999087592739393 1102 70-71 3.169998509796546 1102 72-73 3.16461212248322 1102 74-75 3.12595446187456 1102 76-77 3.4050872830666314 1102 78-79 3.7460862377123227 1102 80-81 4.2178593355539995 1102 82-83 4.4509881000227836 1102 84-85 4.564078780741351 1102 86-87 4.755355369850665 1102 88-89 5.010699719870306 1102 90-91 5.335890257460516 1102 92-93 5.3602599615243065 1102 94-95 5.581684357586386 1102 96-97 5.568288912509502 1102 98-99 5.991113104927273 1102 100-101 5.659609935447319 1103 1 0.4720753350078333 1103 2 0.5303872088263013 1103 3 0.3903563391833984 1103 4 0.4291122621334438 1103 5 0.2553717808888365 1103 6 0.3752046195259737 1103 7 0.5219159480929534 1103 8 0.3945227413581023 1103 9 0.5473542146467736 1103 10-11 0.37081606235779674 1103 12-13 0.6190168960417068 1103 14-15 0.7300285113595422 1103 16-17 0.8240928449815357 1103 18-19 0.9222628907923038 1103 20-21 0.8772199176110007 1103 22-23 0.8394840269175319 1103 24-25 0.7534870064692498 1103 26-27 0.7090278637386618 1103 28-29 0.9352671958936654 1103 30-31 1.0110527900741957 1103 32-33 0.9374372966595175 1103 34-35 1.0133869864030345 1103 36-37 1.0304275336459767 1103 38-39 1.2670612482348034 1103 40-41 1.244836167933407 1103 42-43 1.3565277350695695 1103 44-45 1.1697988570163886 1103 46-47 1.3548272860076693 1103 48-49 1.34816667400478 1103 50-51 1.4321005088241243 1103 52-53 1.6556466526758946 1103 54-55 1.7896211662386605 1103 56-57 1.7326903035583605 1103 58-59 2.0125058435571646 1103 60-61 2.211970923971432 1103 62-63 2.299336319947461 1103 64-65 2.370025892909265 1103 66-67 2.42367391615684 1103 68-69 2.5379740664693635 1103 70-71 2.6723346585151297 1103 72-73 2.7112062308755505 1103 74-75 2.791109929104305 1103 76-77 2.9127933651268343 1103 78-79 3.0313323769082814 1103 80-81 3.452369541325538 1103 82-83 3.787343832229965 1103 84-85 3.8701908579160325 1103 86-87 3.8856305944604443 1103 88-89 4.464728204944624 1103 90-91 4.83241708642586 1103 92-93 4.860418844478801 1103 94-95 4.885940040110295 1103 96-97 5.1134939595015965 1103 98-99 5.1643022532732346 1103 100-101 4.69398040702435 1104 1 0.5420904591675502 1104 2 0.49803202904744026 1104 3 0.4315175560390685 1104 4 0.4114613450468809 1104 5 0.42275089578131997 1104 6 0.4528432838797656 1104 7 0.5204954767855909 1104 8 0.5707147768851257 1104 9 0.4174988480284938 1104 10-11 0.6399518611848336 1104 12-13 0.6153924570516622 1104 14-15 0.8121845104784526 1104 16-17 0.8599591308891235 1104 18-19 1.0538492263401693 1104 20-21 0.8870634051753612 1104 22-23 0.9560991160694385 1104 24-25 0.9477837822107986 1104 26-27 0.946610171421888 1104 28-29 0.84116846994187 1104 30-31 1.0302239900553118 1104 32-33 0.9852551596885206 1104 34-35 1.2554153348605297 1104 36-37 1.2613991674879088 1104 38-39 1.1972688563361658 1104 40-41 1.4180272181283087 1104 42-43 1.441196232762593 1104 44-45 1.4312730102404458 1104 46-47 1.5696051044312185 1104 48-49 1.7426980315603728 1104 50-51 1.8058855608834854 1104 52-53 1.8542515521956169 1104 54-55 2.090901212968191 1104 56-57 2.1451085858943664 1104 58-59 2.2968678262577953 1104 60-61 2.373269398968379 1104 62-63 2.488135591470378 1104 64-65 2.7677630216396665 1104 66-67 2.8780315032141743 1104 68-69 3.117138422227363 1104 70-71 3.1489187618235626 1104 72-73 3.2243403048797 1104 74-75 3.334478801262872 1104 76-77 3.5468953951863433 1104 78-79 3.840549165708932 1104 80-81 4.09357245287379 1104 82-83 4.551324337994455 1104 84-85 4.761745814274274 1104 86-87 4.785989375323368 1104 88-89 5.251303988981849 1104 90-91 5.4368217275171595 1104 92-93 5.4623213123325876 1104 94-95 5.6524849169732825 1104 96-97 5.831209336189545 1104 98-99 5.885892581284594 1104 100-101 5.580808325947837 1105 1 0.6955121444465462 1105 2 0.6405806140260459 1105 3 0.5709019666694175 1105 4 0.6810717748253623 1105 5 0.5003214359684875 1105 6 0.5616614516391181 1105 7 0.513760359230325 1105 8 0.6045595392674414 1105 9 0.7766926209033826 1105 10-11 0.6617957706647033 1105 12-13 0.6048225588844574 1105 14-15 0.9559972187248889 1105 16-17 0.9298767461364008 1105 18-19 0.913904336959753 1105 20-21 0.859849959308896 1105 22-23 0.9154892439579427 1105 24-25 0.8571797679457731 1105 26-27 1.0693055774643767 1105 28-29 1.2108398213236171 1105 30-31 1.2730476595040656 1105 32-33 1.1461561433380965 1105 34-35 1.2071548809477193 1105 36-37 1.3121128875015913 1105 38-39 1.3549217812760617 1105 40-41 1.425757700955451 1105 42-43 1.6841499464029397 1105 44-45 1.5466807866487926 1105 46-47 1.5775017157841944 1105 48-49 1.8505880821253058 1105 50-51 1.6164772502395444 1105 52-53 1.8936085481045062 1105 54-55 2.101123155055177 1105 56-57 2.0679103704287165 1105 58-59 2.061925772075057 1105 60-61 2.3906925088756417 1105 62-63 2.5951617268381817 1105 64-65 2.7371030532251694 1105 66-67 2.866671264401493 1105 68-69 2.80281669055422 1105 70-71 3.1469863754306395 1105 72-73 3.311955880190517 1105 74-75 3.368036294854388 1105 76-77 3.5317212296951546 1105 78-79 3.840128806646373 1105 80-81 4.275364391539792 1105 82-83 4.709886635020073 1105 84-85 5.003591926304416 1105 86-87 4.950637271887857 1105 88-89 5.224655473828772 1105 90-91 5.530895930029942 1105 92-93 5.684555823014957 1105 94-95 5.7833552992329125 1105 96-97 6.299152622863176 1105 98-99 6.369553641601669 1105 100-101 5.827008692414717 1106 1 0.2967368492444038 1106 2 0.27737443343695745 1106 3 0.3252191608224422 1106 4 0.2741493394625607 1106 5 0.4023757795003746 1106 6 0.1900623120052103 1106 7 0.4157532480411774 1106 8 0.3741745693363612 1106 9 0.3281170535446165 1106 10-11 0.26754178708190324 1106 12-13 0.23641248031021433 1106 14-15 0.7325127360419472 1106 16-17 0.473121116444986 1106 18-19 0.5699137307528233 1106 20-21 0.5161183408549164 1106 22-23 0.4856705353850046 1106 24-25 0.537389152959598 1106 26-27 0.6442408305261367 1106 28-29 0.37070110923898625 1106 30-31 0.6925705074914177 1106 32-33 0.9444858918127039 1106 34-35 0.7021946070352456 1106 36-37 0.8308126811893146 1106 38-39 0.7259722180435375 1106 40-41 0.8573937134219776 1106 42-43 0.6048140963524631 1106 44-45 0.8513535402592538 1106 46-47 1.3000907153891248 1106 48-49 1.31309192304537 1106 50-51 0.935497486401264 1106 52-53 1.293551483006393 1106 54-55 1.260576208037925 1106 56-57 1.1289480772896958 1106 58-59 0.40704670618682215 1106 60-61 1.81433062836118 1106 62-63 2.260275198590957 1106 64-65 2.2500853630447537 1106 66-67 2.3783630250792527 1106 68-69 1.8341125072435673 1106 70-71 2.130191678095141 1106 72-73 2.590310649515832 1106 74-75 2.8716379804695897 1106 76-77 2.808969814041756 1106 78-79 3.215323047461087 1106 80-81 3.7201056232679868 1106 82-83 4.125965823817754 1106 84-85 4.1902957584452345 1106 86-87 4.309677700315053 1106 88-89 4.257599593928855 1106 90-91 4.835399683157551 1106 92-93 4.973443492564144 1106 94-95 4.952873757597338 1106 96-97 5.339161841071331 1106 98-99 5.311926671758378 1106 100-101 4.741134499007929 1107 1 0.45080398410122413 1107 2 0.2858046064830724 1107 3 0.4057396454908506 1107 4 0.42357264846517495 1107 5 0.4445845345216526 1107 6 0.45136041404073524 1107 7 0.4245653606259481 1107 8 0.335818701496315 1107 9 0.43191588807452774 1107 10-11 0.3679582830550103 1107 12-13 0.48461906124823173 1107 14-15 0.5630195245625629 1107 16-17 0.7704796784892025 1107 18-19 0.6206034510120517 1107 20-21 0.5636560618203603 1107 22-23 0.5040332435359574 1107 24-25 0.6507492294539716 1107 26-27 0.7597992773674562 1107 28-29 0.6278471159413286 1107 30-31 0.6731548040095845 1107 32-33 0.9092255751755118 1107 34-35 1.0562767355696607 1107 36-37 1.0088763541922887 1107 38-39 0.8664597609812432 1107 40-41 0.8934248958353734 1107 42-43 1.1301402143850865 1107 44-45 1.219818065683853 1107 46-47 1.1741117735213464 1107 48-49 1.3749201048157644 1107 50-51 1.4317080340098478 1107 52-53 1.4340065322493096 1107 54-55 1.6115969425639918 1107 56-57 1.688632007904463 1107 58-59 1.758977375066749 1107 60-61 1.6733280659061869 1107 62-63 1.849636740739495 1107 64-65 2.0803902026977994 1107 66-67 2.203165562115963 1107 68-69 2.382010985851071 1107 70-71 2.2520928276178154 1107 72-73 2.637473195841526 1107 74-75 2.878808218818051 1107 76-77 2.9741137500196437 1107 78-79 3.1818865919309793 1107 80-81 3.5086371012298336 1107 82-83 3.8295249591261573 1107 84-85 4.015553429008854 1107 86-87 4.406701211333079 1107 88-89 4.508042251207765 1107 90-91 4.781093625379103 1107 92-93 4.773103229238785 1107 94-95 4.915156008254808 1107 96-97 5.01352921766793 1107 98-99 5.21768009629438 1107 100-101 5.168849336116926 1108 1 0.25611675909038567 1108 2 0.41960910092779713 1108 3 0.3384701907819938 1108 4 0.23874744839071127 1108 5 0.14411760734971324 1108 6 0.2581707542370779 1108 7 0.28805621177688323 1108 8 0.3688092800787146 1108 9 0.193236019290552 1108 10-11 0.4131199105702308 1108 12-13 0.4621462129466707 1108 14-15 0.3444000956113271 1108 16-17 0.48374050179191386 1108 18-19 0.5872098499799137 1108 20-21 0.667079264336067 1108 22-23 0.6447187919067758 1108 24-25 0.5567722234932759 1108 26-27 0.6579001889415608 1108 28-29 0.7259198336432107 1108 30-31 0.7366519227790818 1108 32-33 0.7656497384484382 1108 34-35 0.7852227428467629 1108 36-37 0.8851585611103232 1108 38-39 0.9031950854877095 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2.553769064954583 2311 90-91 2.617402012939305 2311 92-93 2.830954681759323 2311 94-95 2.86877441189824 2311 96-97 2.8064763110714637 2311 98-99 3.29201958698804 2311 100-101 3.3071865296679572 2312 1 -0.452996617350518 2312 2 -0.32145153113991753 2312 3 -0.39465090708247885 2312 4 -0.45685919676440534 2312 5 -0.4193353066736094 2312 6 -0.1267472795570157 2312 7 -0.32877716744416574 2312 8 -0.3574438288617898 2312 9 -0.413592494681005 2312 10-11 -0.4061616068960916 2312 12-13 -0.5155024392409828 2312 14-15 -0.29857478365023127 2312 16-17 -0.3715346459223525 2312 18-19 -0.28479309681760157 2312 20-21 -0.35880678130665444 2312 22-23 -0.4690739023854533 2312 24-25 -0.4498020072731421 2312 26-27 -0.28476465066456313 2312 28-29 -0.3778984272086561 2312 30-31 -0.3516413154457254 2312 32-33 -0.24272649701581628 2312 34-35 -0.28860050613382526 2312 36-37 -0.2439759889577786 2312 38-39 -0.03403513220436594 2312 40-41 0.1184542106251385 2312 42-43 0.07837478017501809 2312 44-45 0.06272347799075817 2312 46-47 0.049356355270745667 2312 48-49 0.2605477740083515 2312 50-51 0.21956166220511975 2312 52-53 0.2986360180152694 2312 54-55 0.4476848058032772 2312 56-57 0.5012489727969474 2312 58-59 0.664290555467062 2312 60-61 0.7234667165445181 2312 62-63 0.8501061081217607 2312 64-65 0.8580998532997448 2312 66-67 0.8534878351109292 2312 68-69 1.036404254821246 2312 70-71 1.2721200771911079 2312 72-73 1.2671909703416766 2312 74-75 1.3764200386658025 2312 76-77 1.4278006624227508 2312 78-79 1.6098095639282803 2312 80-81 1.7958393545045581 2312 82-83 2.034978589811754 2312 84-85 2.1843617728336433 2312 86-87 2.3440765128225856 2312 88-89 2.3770643347242277 2312 90-91 2.5316986472307086 2312 92-93 2.747316817565558 2312 94-95 2.931459155963797 2312 96-97 3.0000640645724523 2312 98-99 3.0154913170250666 2312 100-101 2.805243119883409 2313 1 -0.3135877079882796 2313 2 -0.5325446375055023 2313 3 -0.4524938210155973 2313 4 -0.2248353038313482 2313 5 -0.11280739290062769 2313 6 -0.26410879385567654 2313 7 -0.23247672881689851 2313 8 -0.16663394997818415 2313 9 -0.5180799945775476 2313 10-11 -0.2784850503342611 2313 12-13 -0.3234741812666826 2313 14-15 -0.2770484370458064 2313 16-17 -0.22156983974205957 2313 18-19 -0.1475744449568097 2313 20-21 -0.18428097124603937 2313 22-23 -0.36619702353747385 2313 24-25 -0.2668957138768846 2313 26-27 -0.1115601382267144 2313 28-29 -0.03691763518937563 2313 30-31 -0.09562157092118895 2313 32-33 -0.005679478000722327 2313 34-35 -0.021079255796010443 2313 36-37 0.17715782780477696 2313 38-39 0.19013843378243678 2313 40-41 0.4004055567887477 2313 42-43 0.4593679404108997 2313 44-45 0.409422815893528 2313 46-47 0.19663376425712187 2313 48-49 0.448703272394944 2313 50-51 0.340566147078075 2313 52-53 0.5655577499110613 2313 54-55 0.6975771191923599 2313 56-57 0.7124459872958617 2313 58-59 1.0226801431343944 2313 60-61 1.0379647889811743 2313 62-63 1.1242656037777685 2313 64-65 1.009684996613803 2313 66-67 1.0651263017399515 2313 68-69 1.4085314873167292 2313 70-71 1.637235894725901 2313 72-73 1.6696493917945041 2313 74-75 1.623084812495133 2313 76-77 1.8790359906842866 2313 78-79 2.0605106874315986 2313 80-81 2.1913394396102674 2313 82-83 2.268537084423105 2313 84-85 2.4892222084669164 2313 86-87 2.543207588929402 2313 88-89 2.723895289595891 2313 90-91 2.894127607354097 2313 92-93 3.0441207293334287 2313 94-95 3.096569089869231 2313 96-97 3.267173460854501 2313 98-99 3.3845270351535177 2313 100-101 3.054420041518144 2314 1 -0.36192111883931233 2314 2 -0.44758002995750346 2314 3 -0.4321298026257949 2314 4 -0.41697476042398307 2314 5 -0.4161196591714429 2314 6 -0.3364560641615526 2314 7 -0.28442873695731663 2314 8 -0.4270114102923799 2314 9 -0.40005095135781943 2314 10-11 -0.3940799193962121 2314 12-13 -0.3620850084420866 2314 14-15 -0.44883665679321894 2314 16-17 -0.4473837844782125 2314 18-19 -0.350395544872633 2314 20-21 -0.3944870523499162 2314 22-23 -0.3874375540993853 2314 24-25 -0.2811234369760811 2314 26-27 -0.25417838792910885 2314 28-29 -0.2800774673392894 2314 30-31 -0.30056707006003336 2314 32-33 -0.23716132767484766 2314 34-35 -0.24089235410987442 2314 36-37 -0.1414548349424578 2314 38-39 0.025540631711038486 2314 40-41 -0.08337439684975578 2314 42-43 0.14410501871312675 2314 44-45 -0.011759700657691496 2314 46-47 0.004009052773859878 2314 48-49 0.09384862873553601 2314 50-51 0.04524216865189956 2314 52-53 0.20278023506227427 2314 54-55 0.25552384926496075 2314 56-57 0.27525955675166713 2314 58-59 0.6847209262028287 2314 60-61 0.6649919508285471 2314 62-63 0.688540441070483 2314 64-65 0.7849934312615865 2314 66-67 1.0122662713695831 2314 68-69 1.2000601458474698 2314 70-71 1.416443144275977 2314 72-73 1.4888262177180316 2314 74-75 1.5856753617500594 2314 76-77 1.8050823287540112 2314 78-79 1.7411501268046514 2314 80-81 2.030199666674406 2314 82-83 2.4400293759090665 2314 84-85 2.548457833045969 2314 86-87 2.8021001710534854 2314 88-89 2.9012370904658162 2314 90-91 2.7793824447249165 2314 92-93 2.8382878004531378 2314 94-95 3.151929509899187 2314 96-97 2.958734197511724 2314 98-99 3.1123379187097413 2314 100-101 2.8971182823870603 2315 1 -0.532921484621145 2315 2 -0.525454946653241 2315 3 -0.4805150993931484 2315 4 -0.3873198521704424 2315 5 -0.45877249630417793 2315 6 -0.4235015784758076 2315 7 -0.47530418771565763 2315 8 -0.48628989418829605 2315 9 -0.6125017213127322 2315 10-11 -0.3922103482551691 2315 12-13 -0.37044579888289775 2315 14-15 -0.4265233489883329 2315 16-17 -0.5148264378786251 2315 18-19 -0.45525074420260836 2315 20-21 -0.5249422271770783 2315 22-23 -0.4017215282432929 2315 24-25 -0.27961295868200864 2315 26-27 -0.3756844057267301 2315 28-29 -0.4667896413965238 2315 30-31 -0.3347148635224997 2315 32-33 -0.3413913710018548 2315 34-35 -0.5977944706805616 2315 36-37 -0.3667667616480941 2315 38-39 -0.17425413968587833 2315 40-41 -0.22417276586412527 2315 42-43 -0.15436397837223836 2315 44-45 -0.26834482977797336 2315 46-47 -0.14148991201779637 2315 48-49 -0.1728036859523101 2315 50-51 0.22819812591422206 2315 52-53 0.24749635984492713 2315 54-55 0.44472388545588615 2315 56-57 0.5628959196199155 2315 58-59 0.7241390232853426 2315 60-61 0.617210246890739 2315 62-63 0.7968909868035965 2315 64-65 0.8108132631145999 2315 66-67 1.0299494817722916 2315 68-69 1.2360168695265834 2315 70-71 1.3592981823662207 2315 72-73 1.5359337424382318 2315 74-75 1.6459509443870033 2315 76-77 1.5916769703142215 2315 78-79 2.0011108166994624 2315 80-81 2.139467131348649 2315 82-83 2.5095629530265455 2315 84-85 2.6291301534300153 2315 86-87 2.820529203217294 2315 88-89 3.0101597203914174 2315 90-91 2.9619218366821514 2315 92-93 3.0239269205911725 2315 94-95 2.995685043713589 2315 96-97 2.9869107521989235 2315 98-99 3.0659046612201024 2315 100-101 3.011476592270812 2316 1 -0.9499513443519163 2316 2 -0.8180161753134882 2316 3 -0.784694330786202 2316 4 -0.5999415060403521 2316 5 -0.7491710292642608 2316 6 -0.7347969841700319 2316 7 -0.6199023872705922 2316 8 -0.5162516220257629 2316 9 -0.642124174696491 2316 10-11 -0.7113652542425015 2316 12-13 -0.6609848014875794 2316 14-15 -1.1899626256609253 2316 16-17 -0.90933534526458 2316 18-19 -0.9149916072337874 2316 20-21 -0.7492767231576778 2316 22-23 -1.0078707703514098 2316 24-25 -0.9926816102235207 2316 26-27 -0.7175499428146566 2316 28-29 -0.9018284253338109 2316 30-31 -0.7483403474717463 2316 32-33 -0.5424254423167767 2316 34-35 -0.6077402583448617 2316 36-37 -0.450497227420378 2316 38-39 -0.5755794931504497 2316 40-41 -0.6790336955477088 2316 42-43 -0.5837501564455181 2316 44-45 -0.44146928266212626 2316 46-47 -0.2555752416902024 2316 48-49 -0.21463572505620476 2316 50-51 -0.16793002964240245 2316 52-53 -0.16468863921383914 2316 54-55 -0.12803885361987 2316 56-57 0.03572551150996972 2316 58-59 0.2724677387163865 2316 60-61 0.23210242704404394 2316 62-63 0.544189138187285 2316 64-65 0.698512524226377 2316 66-67 0.906035392649045 2316 68-69 0.8586164490839359 2316 70-71 0.8077966423894587 2316 72-73 1.1488337588293405 2316 74-75 1.3223201565902905 2316 76-77 1.3926298734370484 2316 78-79 1.5486160400229316 2316 80-81 1.761857706390213 2316 82-83 1.9303892509481706 2316 84-85 1.7948721659860318 2316 86-87 2.2303358811978775 2316 88-89 2.052484924260291 2316 90-91 2.390941541083915 2316 92-93 2.5965846205823695 2316 94-95 2.5472912648904718 2316 96-97 2.6102065959437084 2316 98-99 2.708868581457679 2316 100-101 2.6047598885869636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6815.0 3 2398.0 4 2741.0 5 3131.0 6 3754.0 7 4817.0 8 6060.0 9 8040.0 10 10206.0 11 12970.0 12 15949.0 13 18962.0 14 22230.0 15 25896.0 16 30666.0 17 35395.0 18 33414.0 19 30786.0 20 32599.0 21 35462.0 22 38930.0 23 43453.0 24 47811.0 25 53294.0 26 59447.0 27 66596.0 28 74032.0 29 82047.0 30 91677.0 31 103039.0 32 117222.0 33 135259.0 34 157129.0 35 185407.0 36 205264.0 37 195815.0 38 98648.0 39 7485.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.123570147364646 18.55467893720562 14.755621720226376 22.56612919520336 2 15.66392003627111 20.13222416229991 39.69894789069621 24.504907910732772 3 19.287288164593782 24.548002083658112 29.604247544667473 26.560462207080636 4 12.932853726144803 16.43223393778542 36.20348260219854 34.43142973387124 5 13.717359568481506 37.6490570396755 33.38069566052383 15.252887731319175 6 32.29705544714945 35.93343204759199 16.857749267367492 14.911763237891066 7 29.008728066965556 33.21308440172869 19.834277931382545 17.943909599923202 8 27.594895681403532 33.76556842359894 19.13278290487644 19.506752990121086 9 27.2451441314188 16.497976285757197 19.612157874425158 36.64472170839884 10-11 25.391566100444802 26.519922792895485 26.41282799415743 21.675683112502288 12-13 26.86114049055365 23.930488621779524 26.9145581388607 22.293812748806122 14-15 23.241678355109915 25.160594814441854 25.66314045164477 25.93458637880346 16-17 22.225823113955077 28.38570017254185 26.608129472438208 22.780347241064867 18-19 22.449536752460464 26.343130362013344 29.44951322951716 21.75781965600903 20-21 22.99375117628831 25.36476819278391 29.756957448305098 21.88452318262268 22-23 22.66034281396396 25.410125688948664 29.87416847950708 22.055363017580294 24-25 22.425430022875137 25.711084043848963 29.82706396126081 22.03642197201509 26-27 21.78039538887257 26.00342022686808 29.706281601213714 22.50990278304564 28-29 22.016076734123175 26.531316410630406 29.533269980725695 21.919336874520727 30-31 21.98012254217021 25.99287483627142 29.297649532434892 22.72935308912348 32-33 21.65670206494302 26.105141696470174 29.338890793810563 22.89926544477624 34-35 22.681880956522317 26.181419499801827 29.084889977502204 22.051809566173656 36-37 22.829043494336254 25.544424730059855 29.106439380168343 22.520092395435544 38-39 22.147030242840817 26.081759497069328 29.108443238542815 22.662767021547044 40-41 22.481236412882073 25.974411670369097 29.231661103125482 22.31269081362335 42-43 22.27555093889006 25.942632123406888 28.80622660615371 22.975590331549338 44-45 22.202860457265498 26.177533843296093 29.144445180904217 22.4751605185342 46-47 22.984641299686505 26.60722283004456 28.120165882041288 22.287969988227648 48-49 22.464194899298118 26.857620868274434 27.805779230606397 22.87240500182105 50-51 22.60755473771083 26.965333614606035 27.970052059206 22.457059588477133 52-53 23.007042644989973 26.583003484025884 27.903469724893565 22.506484146090582 54-55 22.303492383862185 26.973046386233534 27.99480308080668 22.728658149097605 56-57 22.537306802467853 27.05700243283802 27.687815641193332 22.717875123500797 58-59 22.523849672173167 26.436462126195572 27.79212270100136 23.247565500629904 60-61 22.016379469266045 26.92033764303647 28.24662613531188 22.816656752385605 62-63 22.168806413634577 26.842983970664676 28.489414025122034 22.498795590578716 64-65 21.96044920715014 26.970436445151435 28.101507778357437 22.967606569340983 66-67 21.88396835513552 26.93203357189163 28.018741954388464 23.165256118584384 68-69 22.528877679038285 26.41969994248577 28.224354437801363 22.82706794067458 70-71 22.413354401825174 26.679605210484166 27.701906974103192 23.20513341358747 72-73 22.230928171091062 26.76583529078595 27.816005551745054 23.187230986377934 74-75 22.197641326883698 27.33318746345829 27.55828796281316 22.91088324684485 76-77 22.415867326284822 26.703084405402887 27.743541450082958 23.13750681822933 78-79 22.43615616383229 26.982402218246516 27.637808350780187 22.94363326714101 80-81 22.81098984324184 27.007440940710513 27.200175606390818 22.98139360965683 82-83 22.572631240841506 27.033660489099137 27.382657259988104 23.011051010071252 84-85 22.38090464342066 27.216698507147836 27.401042302625044 23.001354546806457 86-87 22.73606639234405 27.088004158073296 27.089762027269774 23.08616742231287 88-89 22.464821610371345 27.113061473233536 26.895261356576118 23.526855559819 90-91 22.33416973187724 27.090164713485976 27.006429813806278 23.569235740830504 92-93 22.678102721123345 26.598757372045238 27.24432806290305 23.478811843928362 94-95 22.380828943526225 26.23118734709132 27.58732114570342 23.800662563679033 96-97 22.68788808623518 25.863046765764896 27.537761748462504 23.911303399537413 98-99 22.442838951239676 26.252601469176746 26.96416862271807 24.340390956865505 100-101 22.82591842907774 25.509604244625255 27.46790658476219 24.196570741534813 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2050.0 1 1329.0 2 852.0 3 1101.0 4 938.0 5 860.5 6 1423.5 7 3008.0 8 4798.5 9 5230.5 10 4105.0 11 2984.5 12 2731.0 13 2745.0 14 2891.0 15 3059.5 16 3187.0 17 3394.5 18 3632.0 19 3900.0 20 4287.5 21 5001.0 22 5892.5 23 6888.5 24 8248.0 25 10111.0 26 12482.0 27 15094.5 28 17187.0 29 19344.0 30 22471.5 31 27401.5 32 34418.5 33 37287.0 34 36739.5 35 38640.5 36 42943.0 37 47326.5 38 50233.0 39 52866.0 40 55341.0 41 58426.5 42 60132.5 43 60889.5 44 62755.5 45 66705.0 46 69538.5 47 71897.0 48 75285.0 49 74614.0 50 72760.5 51 71348.0 52 71118.0 53 73545.0 54 75690.0 55 73822.5 56 69660.5 57 64848.5 58 60116.0 59 54344.0 60 45416.5 61 37946.5 62 32462.5 63 25038.0 64 17642.5 65 13141.5 66 10071.0 67 7843.5 68 6076.5 69 4631.0 70 3599.5 71 2622.5 72 1932.5 73 1516.0 74 1127.5 75 805.5 76 618.0 77 467.0 78 321.5 79 218.0 80 159.0 81 95.5 82 58.0 83 44.0 84 25.5 85 20.5 86 11.5 87 6.5 88 7.5 89 7.0 90 4.5 91 1.0 92 1.0 93 3.5 94 2.5 95 1.0 96 1.0 97 0.0 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30301504243065763 2 0.03287651448134448 3 0.04057303954778639 4 0.04285349141932474 5 0.057248843858410545 6 0.05373314722312226 7 0.028505648394229314 8 0.031688779131584924 9 0.03772247470836346 10-11 0.030192232590887887 12-13 0.031950080908532026 14-15 0.026581517127618835 16-17 0.02038153860187396 18-19 0.024586121740022785 20-21 0.036582248772594285 22-23 0.03057230790281094 24-25 0.04910097935905999 26-27 0.042378397279420915 28-29 0.03560830578579146 30-31 0.03582209814874818 32-33 0.03076234555877247 34-35 0.030406024953844604 36-37 0.029907176106945592 38-39 0.018101086730335617 40-41 0.017625992590431794 42-43 0.018671199698220203 44-45 0.008670468053244751 46-47 0.016081936635744375 48-49 0.013896503592186792 50-51 0.0060336955767785385 52-53 0.0046084131570670725 54-55 0.006532544423677552 56-57 0.004869714934014175 58-59 0.007245185633533284 60-61 0.006556299130672742 62-63 0.0038007531192305755 64-65 0.007601506238461151 66-67 0.002351715992523919 68-69 0.007102657391562137 70-71 0.013373900038292589 72-73 0.01342140945228297 74-75 0.010950919924783096 76-77 0.009216826314134145 78-79 0.005724884385841054 80-81 0.006603808544663125 82-83 0.011497278185672491 84-85 0.003943281361201722 86-87 0.0011877353497595547 88-89 0.018694954405215396 90-91 0.027840516598363965 92-93 0.01301757943336472 94-95 0.021117934518724884 96-97 0.01750721905545584 98-99 0.012637504121441665 100-101 0.10613603085451381 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2104846.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.858068818121026 #Duplication Level Percentage of deduplicated Percentage of total 1 73.50695263248987 34.44393845063573 2 13.389879782955402 12.548478166721836 3 4.982105625626694 7.003555447942906 4 2.4650196411899508 4.6202423993959485 5 1.394865882862608 3.268036076561261 6 0.9196238817358701 2.5855079482300196 7 0.6125313692450267 2.009142593733895 8 0.44879748683122067 1.682382681866968 9 0.32712880119199433 1.3795761490779515 >10 1.765980390244093 15.118663247375528 >50 0.12032197166827181 3.846648375300177 >100 0.057588820207595065 5.086859387572644 >500 0.004499593389606591 1.482700251574403 >1k 0.004397329903479165 3.6145071416281156 >5k 2.045269722548449E-4 0.7478191495724296 >10k+ 1.0226348612742245E-4 0.5619425328101937 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11727 0.557142897865212 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8941 0.42478167048800713 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6665 0.3166502442458973 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3331 0.1582538580019631 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3283 0.15597340613042474 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3182 0.15117495531739614 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2851 0.13544933928657962 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2794 0.13274130268912784 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2726 0.12951066253778187 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2491 0.11834595025004203 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2429 0.11540036658263834 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2370 0.1125973111572058 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2301 0.10931916159186944 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2205 0.10475825784879272 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2152 0.10224025890730248 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.750941399038219E-5 2 0.0 0.0 0.0 0.0 4.750941399038219E-5 3 0.0 0.0 0.0 0.0 4.750941399038219E-5 4 0.0 0.0 0.0 0.0 4.750941399038219E-5 5 0.0 0.0 0.0 0.0 4.750941399038219E-5 6 0.0 0.0 0.0 0.0 4.750941399038219E-5 7 0.0 0.0 0.0 0.0 4.750941399038219E-5 8 0.0 0.0 0.0 0.0 4.750941399038219E-5 9 0.0 0.0 0.0 0.0 4.750941399038219E-5 10-11 0.0 0.0 0.0 2.3754706995191096E-5 4.750941399038219E-5 12-13 0.0 0.0 0.0 4.750941399038219E-5 1.1877353497595549E-4 14-15 0.0 0.0 0.0 4.750941399038219E-5 1.9003765596152877E-4 16-17 0.0 0.0 0.0 7.126412098557329E-5 2.6130177694710206E-4 18-19 0.0 0.0 0.0 1.4252824197114657E-4 3.3256589793267537E-4 20-21 4.750941399038219E-5 0.0 0.0 2.1379236295671986E-4 3.5632060492786646E-4 22-23 4.750941399038219E-5 0.0 0.0 6.888865028605418E-4 3.8007531192305754E-4 24-25 4.750941399038219E-5 0.0 0.0 0.001449037126706657 3.8007531192305754E-4 26-27 4.750941399038219E-5 0.0 0.0 0.002803055425432549 4.2758472591343977E-4 28-29 4.750941399038219E-5 0.0 0.0 0.006675072665648698 4.7509413990382194E-4 30-31 4.750941399038219E-5 0.0 0.0 0.01731718139949431 4.7509413990382194E-4 32-33 4.750941399038219E-5 0.0 0.0 0.0360121358047097 5.226035538942041E-4 34-35 4.750941399038219E-5 0.0 0.0 0.0637813882820881 5.226035538942041E-4 36-37 4.750941399038219E-5 0.0 0.0 0.11276359410617215 5.226035538942041E-4 38-39 4.750941399038219E-5 0.0 0.0 0.18725835524309142 5.463582608893952E-4 40-41 4.750941399038219E-5 0.0 0.0 0.26427111532150094 5.701129678845863E-4 42-43 4.750941399038219E-5 0.0 0.0 0.3496217775552226 5.701129678845863E-4 44-45 4.750941399038219E-5 0.0 0.0 0.453097281226275 5.938676748797774E-4 46-47 4.750941399038219E-5 0.0 0.0 0.5727497403610525 6.176223818749686E-4 48-49 4.750941399038219E-5 0.0 0.0 0.6997661586643393 6.651317958653507E-4 50-51 4.750941399038219E-5 0.0 0.0 0.8468790590855577 6.651317958653507E-4 52-53 4.750941399038219E-5 0.0 0.0 0.986129151491368 7.126412098557329E-4 54-55 4.750941399038219E-5 0.0 0.0 1.1366152203059037 7.601506238461151E-4 56-57 4.750941399038219E-5 0.0 0.0 1.3072927900663518 7.601506238461151E-4 58-59 4.750941399038219E-5 0.0 0.0 1.4904178262922798 7.601506238461151E-4 60-61 4.750941399038219E-5 0.0 0.0 1.696418645354577 8.076600378364973E-4 62-63 4.750941399038219E-5 0.0 0.0 1.9091658012035087 8.076600378364973E-4 64-65 4.750941399038219E-5 0.0 0.0 2.1182547321751803 8.076600378364973E-4 66-67 4.750941399038219E-5 0.0 0.0 2.3614791770989423 8.076600378364973E-4 68-69 4.750941399038219E-5 0.0 0.0 2.6329004592259957 8.076600378364973E-4 70-71 4.750941399038219E-5 0.0 0.0 2.921472639803577 8.314147448316885E-4 72-73 4.750941399038219E-5 0.0 0.0 3.2209482308919513 8.789241588220706E-4 74-75 4.750941399038219E-5 0.0 0.0 3.516860615931047 9.026788658172617E-4 76-77 4.750941399038219E-5 0.0 0.0 3.8339621996098527 9.026788658172617E-4 78-79 4.750941399038219E-5 0.0 0.0 4.2015187809464445 9.026788658172617E-4 80-81 4.750941399038219E-5 0.0 0.0 4.58689139252943 9.026788658172617E-4 82-83 9.501882798076439E-5 0.0 0.0 4.984022584075035 9.026788658172617E-4 84-85 9.501882798076439E-5 2.3754706995191096E-5 0.0 5.393553732672129 9.026788658172617E-4 86-87 9.501882798076439E-5 4.750941399038219E-5 0.0 5.805460351968742 9.264335728124528E-4 88-89 9.501882798076439E-5 4.750941399038219E-5 0.0 6.238152339886149 9.501882798076439E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7350 0.0 36.891113 1 GTATCAA 11735 0.0 36.6692 1 AGGTAAG 1235 0.0 33.09323 2 TAAGGTG 1485 0.0 29.762144 5 TATCAAC 14545 0.0 29.504055 2 TCAACGC 14880 0.0 28.903694 4 ATCAACG 14895 0.0 28.874586 3 GGTAAGG 1545 0.0 28.60633 3 GTAAGGT 1525 0.0 28.358236 4 CAACGCA 15200 0.0 28.263931 5 AACGCAG 15645 0.0 27.54589 6 GTACATG 10585 0.0 26.201666 1 AAGGTAA 1480 0.0 26.080751 1 TACATGG 10590 0.0 25.713806 2 ACGCAGA 17410 0.0 24.639477 7 CGCAGAG 17485 0.0 24.316435 8 ACATGGG 10715 0.0 24.21632 3 GAGTACT 9485 0.0 23.562056 12-13 GCAGAGT 18305 0.0 22.551315 9 TAAGGTA 720 0.0 22.441614 4 >>END_MODULE