FastQCFastQC Report
Thu 26 May 2016
SRR938513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938513_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104846
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112840.5360962274674727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94350.448251320999256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68950.3275774094636852No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA34640.16457261006268392No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32190.15293280363504028No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC32000.152030124769223No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29370.13953514888975252No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA28490.13535432045859888No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27840.13226620854922402No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG26840.12751526715018582No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA23610.11216972643129236No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22630.1075138038602349No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA22110.10504331433273503No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA21980.10442569195086007No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21250.10095750472956216No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73900.039.7772641
GTATCAA117550.036.012911
AGGTAAG11700.031.3029582
TATCAAC149700.028.150912
ATCAACG153350.027.437453
TCAACGC153600.027.3927924
CGCGAAA350.00839908327.13953272-73
CAACGCA156600.026.9894665
AACGCAG161050.026.2169086
TAAGGTA7100.026.1150284
TAAGGTG14700.025.8736025
AAGGTAA14350.024.5284791
GTGTAAG9200.024.2996981
GTAAGGT15350.024.1585274
GTACATG107200.023.9157941
TACATGG107150.023.5268752
ACGCAGA181450.023.0861767
CGCAGAG181700.022.9498568
ACATGGG106250.022.6862773
GGTAAGG16300.022.4588433
Produced by FastQC (version 0.11.5)