##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938513_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2104846 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78279456074221 31.0 31.0 34.0 30.0 34.0 2 32.00912750861583 31.0 31.0 34.0 30.0 34.0 3 32.06524990426853 33.0 31.0 34.0 30.0 34.0 4 35.75327126070031 37.0 35.0 37.0 35.0 37.0 5 35.625903747827635 37.0 35.0 37.0 33.0 37.0 6 35.67174415610453 37.0 35.0 37.0 33.0 37.0 7 35.607087644416744 37.0 35.0 37.0 33.0 37.0 8 35.584238466852206 37.0 35.0 37.0 33.0 37.0 9 37.14260140646869 39.0 37.0 39.0 33.0 39.0 10-11 37.076426018815624 39.0 37.0 39.0 33.0 39.0 12-13 36.9714142982432 39.0 37.0 39.0 33.0 39.0 14-15 37.97888064019885 40.0 37.0 41.0 33.0 41.0 16-17 37.90619408735841 40.0 37.0 41.0 33.0 41.0 18-19 37.93949010996529 40.0 37.0 41.0 33.0 41.0 20-21 37.9377871350208 40.0 37.0 41.0 32.5 41.0 22-23 37.95940320574522 40.0 37.0 41.0 33.0 41.0 24-25 37.86995865730795 40.0 37.0 41.0 32.5 41.0 26-27 37.68142087354609 40.0 37.0 41.0 32.0 41.0 28-29 37.542115432672986 39.5 36.5 41.0 32.0 41.0 30-31 37.25821960371448 39.0 36.0 41.0 31.0 41.0 32-33 36.99304319650939 39.0 35.5 40.5 31.0 41.0 34-35 36.79652311855594 39.0 35.0 40.5 30.0 41.0 36-37 36.7711932369399 39.0 35.0 41.0 30.0 41.0 38-39 36.603513748749315 39.0 35.0 41.0 30.0 41.0 40-41 36.398452665895746 39.0 35.0 40.0 30.0 41.0 42-43 36.13208353485243 38.5 35.0 40.0 30.0 41.0 44-45 35.805434459338116 38.0 35.0 40.0 29.0 41.0 46-47 35.37662565337321 38.0 34.5 40.0 27.5 41.0 48-49 34.91866649626624 38.0 34.0 40.0 26.0 41.0 50-51 34.51364066539785 38.0 33.5 40.0 24.0 41.0 52-53 33.98144068497173 37.5 33.0 40.0 23.0 41.0 54-55 33.64852939359935 37.0 33.0 40.0 23.0 41.0 56-57 33.26540706541002 37.0 32.0 39.5 22.0 41.0 58-59 32.795629941572926 36.0 31.5 39.0 20.0 40.5 60-61 32.3705653525246 35.5 31.0 39.0 18.5 40.0 62-63 31.888272586212956 35.0 31.0 38.5 17.0 40.0 64-65 31.385016290978058 35.0 30.0 38.0 13.0 40.0 66-67 30.885911843431778 34.5 29.5 38.0 9.0 40.0 68-69 30.317736546996787 34.0 29.0 37.0 7.5 39.0 70-71 29.744690822986577 34.0 28.0 36.5 6.0 39.0 72-73 29.178061957976972 34.0 27.5 36.0 2.0 39.0 74-75 28.572542836863125 33.0 26.0 35.0 2.0 38.0 76-77 27.32780759257447 31.5 25.5 34.0 2.0 36.0 78-79 27.9590065021384 33.0 26.0 35.0 2.0 37.0 80-81 27.683091779636136 33.0 26.0 35.0 2.0 36.0 82-83 27.21449502718964 33.0 25.5 35.0 2.0 36.0 84-85 26.643169619060018 32.0 25.0 35.0 2.0 35.5 86-87 26.035871745486368 32.0 23.5 34.5 2.0 35.0 88-89 25.29427568572713 31.0 20.0 34.0 2.0 35.0 90-91 24.759496181668396 31.0 18.5 34.0 2.0 35.0 92-93 24.004438567002055 31.0 11.0 34.0 2.0 35.0 94-95 23.233821144159716 30.5 2.0 34.0 2.0 35.0 96-97 22.27326203437211 30.0 2.0 34.0 2.0 35.0 98-99 21.126527308886256 29.5 2.0 34.0 2.0 35.0 100-101 18.043007184373582 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18646820733249214 1101 2 0.3403207356023614 1101 3 0.2549980674320764 1101 4 0.2012377807586816 1101 5 0.2264139351522516 1101 6 0.20664379643181974 1101 7 0.26852185961372044 1101 8 0.2508092247451401 1101 9 0.35357376817948705 1101 10-11 0.3454840700929651 1101 12-13 0.3420565164074958 1101 14-15 0.4333964596048929 1101 16-17 0.54659716157866 1101 18-19 0.6037244796280845 1101 20-21 0.591497066051069 1101 22-23 0.6944939080663559 1101 24-25 0.5966860136793386 1101 26-27 0.494226037269371 1101 28-29 0.4958912701760312 1101 30-31 0.685587186626087 1101 32-33 0.5941523741847305 1101 34-35 0.4996788656525126 1101 36-37 0.6640150672413228 1101 38-39 0.8587932015811361 1101 40-41 0.8260896469616696 1101 42-43 0.7041436766260816 1101 44-45 0.915581848497034 1101 46-47 1.0054028080797437 1101 48-49 1.051868337701741 1101 50-51 1.082419558914161 1101 52-53 1.1720988670169348 1101 54-55 1.1875957438707232 1101 56-57 1.3161530133810473 1101 58-59 1.3420237929839658 1101 60-61 1.4573153257513596 1101 62-63 1.5044303113106814 1101 64-65 1.5103716476943312 1101 66-67 1.610766028982873 1101 68-69 1.6569484473624456 1101 70-71 1.8526322824390427 1101 72-73 1.9859701361546165 1101 74-75 2.062215817569413 1101 76-77 1.8604280121945003 1101 78-79 2.1672085998442476 1101 80-81 2.4699511819124993 1101 82-83 2.462766701009066 1101 84-85 2.62080741496154 1101 86-87 2.7481328847560285 1101 88-89 2.7653058769144288 1101 90-91 3.0836486384749513 1101 92-93 3.2671473439327947 1101 94-95 3.3284271972245385 1101 96-97 3.637259944049216 1101 98-99 4.011006265404287 1101 100-101 4.404849864140495 1102 1 0.3604761308118185 1102 2 0.3146679240804815 1102 3 0.2917984701724663 1102 4 0.25505615359997336 1102 5 0.24045184527985697 1102 6 0.2568911315599749 1102 7 0.2967881060295525 1102 8 0.33928844202795716 1102 9 0.48765567331661686 1102 10-11 0.3598880580983064 1102 12-13 0.4486081612955317 1102 14-15 0.6226395703366094 1102 16-17 0.4699571983506061 1102 18-19 0.5568161687300162 1102 20-21 0.6092619663509353 1102 22-23 0.6497114754268765 1102 24-25 0.5212526300034597 1102 26-27 0.41787226048384696 1102 28-29 0.38637215095174327 1102 30-31 0.5138490554290271 1102 32-33 0.2688568853148112 1102 34-35 0.4836469528545635 1102 36-37 0.555178855627517 1102 38-39 0.5740251056238606 1102 40-41 0.5482058944718986 1102 42-43 0.6387180632130693 1102 44-45 0.4941567166214398 1102 46-47 0.5333084486339459 1102 48-49 0.639844129502471 1102 50-51 0.7807380083636772 1102 52-53 0.7668052386324931 1102 54-55 0.7370698831225795 1102 56-57 0.7634761031002242 1102 58-59 0.8000736758828211 1102 60-61 0.8729018164379951 1102 62-63 0.9538907705222748 1102 64-65 0.9209731317051002 1102 66-67 1.0410935838671946 1102 68-69 1.1314149257917592 1102 70-71 1.1641536123578398 1102 72-73 1.3743475739377935 1102 74-75 1.4237207314398788 1102 76-77 1.2576673800672005 1102 78-79 1.7372799549343192 1102 80-81 1.952648119772288 1102 82-83 2.0021006472082092 1102 84-85 2.015215014497514 1102 86-87 2.134626854594277 1102 88-89 2.3653392562126996 1102 90-91 2.6475251241273234 1102 92-93 2.8068517456225557 1102 94-95 2.9969713126184168 1102 96-97 3.2993738933126835 1102 98-99 3.6764482948656187 1102 100-101 4.298674146759183 1103 1 0.18849542448134216 1103 2 0.23935636858013254 1103 3 0.2495335341396725 1103 4 0.15437197358092902 1103 5 0.09432507013805491 1103 6 0.16823858322229768 1103 7 0.054257157493140085 1103 8 0.16681400821020986 1103 9 0.2608173923577297 1103 10-11 0.2744851060840716 1103 12-13 0.3823130840794562 1103 14-15 0.4635009540156574 1103 16-17 0.4366430137772639 1103 18-19 0.5079464631033801 1103 20-21 0.4389233955729779 1103 22-23 0.4196013441802364 1103 24-25 0.26673404018024627 1103 26-27 0.2843898773430382 1103 28-29 0.32357301626868207 1103 30-31 0.4667244746316115 1103 32-33 0.5819794787814558 1103 34-35 0.46508507648309916 1103 36-37 0.5127012130986373 1103 38-39 0.6053095216384392 1103 40-41 0.7712972288816999 1103 42-43 0.7785128092073634 1103 44-45 0.8229166015218752 1103 46-47 0.8186270425974271 1103 48-49 0.8866421463119778 1103 50-51 0.9747180522505658 1103 52-53 1.2474975956895875 1103 54-55 1.2807166350788677 1103 56-57 1.1297209795130883 1103 58-59 1.3304472319628147 1103 60-61 1.3624150239070403 1103 62-63 1.515787843349667 1103 64-65 1.3077506541660817 1103 66-67 1.3737127202063704 1103 68-69 1.5407176004077527 1103 70-71 1.3462132943849134 1103 72-73 1.3357338406541395 1103 74-75 1.6329318025283577 1103 76-77 1.4581724933036533 1103 78-79 1.6441641929171773 1103 80-81 1.8894924041261945 1103 82-83 1.9379284946805697 1103 84-85 1.998857833194986 1103 86-87 2.1015305518575573 1103 88-89 2.377047429110476 1103 90-91 2.3275653882767067 1103 92-93 2.4970030725798686 1103 94-95 2.456397677873248 1103 96-97 2.8292252575324497 1103 98-99 3.2508174208541085 1103 100-101 3.5878861494089556 1104 1 0.2180278539052125 1104 2 0.18764216603046435 1104 3 0.16905343548845764 1104 4 0.1355144671595525 1104 5 0.016438597705722202 1104 6 0.1416498580965211 1104 7 0.040455624229821296 1104 8 0.14473893022933737 1104 9 0.2386547948570552 1104 10-11 0.24780419045288227 1104 12-13 0.24940584297006296 1104 14-15 0.4513480328304311 1104 16-17 0.4530816871780132 1104 18-19 0.3165206226209847 1104 20-21 0.3945114185758598 1104 22-23 0.2677264597256723 1104 24-25 0.3803641236876345 1104 26-27 0.3328737245831235 1104 28-29 0.46726212136684353 1104 30-31 0.5154880893254514 1104 32-33 0.5200953113576006 1104 34-35 0.6482717900366239 1104 36-37 0.6215280483534684 1104 38-39 0.7385931458512545 1104 40-41 0.7383436712623919 1104 42-43 0.8390406699156188 1104 44-45 0.8308152381840941 1104 46-47 0.9087244547730648 1104 48-49 0.9062157885793809 1104 50-51 1.0204317591840848 1104 52-53 1.1681933562047107 1104 54-55 1.316284469590265 1104 56-57 1.3622866486538996 1104 58-59 1.5580449391480684 1104 60-61 1.3870023546206909 1104 62-63 1.3291873768411229 1104 64-65 1.440618314818483 1104 66-67 1.4977903234695589 1104 68-69 1.5007243757952935 1104 70-71 1.6755170421870922 1104 72-73 1.7251566150032538 1104 74-75 1.8138203911630129 1104 76-77 1.5816468018994492 1104 78-79 1.8609461100076743 1104 80-81 2.0551378458342633 1104 82-83 2.299784341181166 1104 84-85 2.4455574622695586 1104 86-87 2.6390847844588734 1104 88-89 2.796815876061846 1104 90-91 2.8667606118743265 1104 92-93 3.1938752822805725 1104 94-95 3.3813614900268583 1104 96-97 3.5462879254420905 1104 98-99 3.8138194288862373 1104 100-101 4.433367241259216 1105 1 0.2657960144008271 1105 2 0.2442861354946828 1105 3 0.30358754924314724 1105 4 0.19877686587738452 1105 5 0.11076211016418824 1105 6 0.1520201208560863 1105 7 0.20734895050249236 1105 8 0.20957404356695264 1105 9 0.2442136568415023 1105 10-11 0.36837718156301946 1105 12-13 0.34328087870292734 1105 14-15 0.43157756668643543 1105 16-17 0.3812998708707056 1105 18-19 0.46279456363949834 1105 20-21 0.4022674416135956 1105 22-23 0.3985621635342014 1105 24-25 0.3291091386602929 1105 26-27 0.4810252225516223 1105 28-29 0.5396351115397238 1105 30-31 0.5543549006418758 1105 32-33 0.5187927606169396 1105 34-35 0.6190141900666148 1105 36-37 0.7507776816522806 1105 38-39 0.7047371364037218 1105 40-41 0.7169093424075825 1105 42-43 0.7036763821594505 1105 44-45 0.7545399850079733 1105 46-47 0.7475354993911125 1105 48-49 0.799857447708952 1105 50-51 0.863579719013309 1105 52-53 0.9341670005818798 1105 54-55 0.9963691974815845 1105 56-57 1.1185641512430706 1105 58-59 1.3258246747483042 1105 60-61 1.218379792070607 1105 62-63 1.3164123509494452 1105 64-65 1.3924869678418368 1105 66-67 1.58415208532233 1105 68-69 1.5426096182398474 1105 70-71 1.540050124960807 1105 72-73 1.6462671221053036 1105 74-75 1.8270724800710632 1105 76-77 1.5851694764515578 1105 78-79 1.968398930912219 1105 80-81 2.201102961186976 1105 82-83 2.2992791605017473 1105 84-85 2.43771661761496 1105 86-87 2.545915267754637 1105 88-89 2.6451276447097136 1105 90-91 2.9365758319653352 1105 92-93 3.2221923464216466 1105 94-95 3.1045034982840782 1105 96-97 3.448035906828199 1105 98-99 3.804489285345568 1105 100-101 4.03278848602816 1106 1 0.239708485319575 1106 2 0.24519478744147705 1106 3 0.26217584650841275 1106 4 0.19256554942946735 1106 5 0.12332082137089628 1106 6 0.09299725092061095 1106 7 0.17621335505014457 1106 8 0.20295010256348434 1106 9 0.2077007732981997 1106 10-11 0.34226770436711007 1106 12-13 0.1284373687604372 1106 14-15 0.11412881553415843 1106 16-17 0.2550674842327183 1106 18-19 0.44799056030338846 1106 20-21 0.4962295152714731 1106 22-23 0.3681706091687289 1106 24-25 0.33370726868092504 1106 26-27 0.40442191277593764 1106 28-29 0.5259548516740438 1106 30-31 0.5200170752610731 1106 32-33 0.5472616601384672 1106 34-35 0.5009113850975595 1106 36-37 0.7025006086528478 1106 38-39 0.5595832801199734 1106 40-41 0.611345495342043 1106 42-43 0.764077593695383 1106 44-45 0.2462743250281605 1106 46-47 0.21688715208414777 1106 48-49 0.7353102373143301 1106 50-51 0.5205745831544846 1106 52-53 0.8407163179955148 1106 54-55 0.796064118687859 1106 56-57 0.5669882766106937 1106 58-59 1.0460301091015012 1106 60-61 1.0131671148395824 1106 62-63 1.20877660186029 1106 64-65 0.6720861952682036 1106 66-67 0.8512013807878489 1106 68-69 0.8711992323919908 1106 70-71 0.9905505419749865 1106 72-73 1.4101317461341019 1106 74-75 1.7642942877177852 1106 76-77 1.6612833432703944 1106 78-79 1.8335058182305985 1106 80-81 1.7433464974672077 1106 82-83 2.010867765040622 1106 84-85 2.244127661906255 1106 86-87 2.5517929728597686 1106 88-89 2.8676771377082666 1106 90-91 2.909307494315538 1106 92-93 2.5611173277657535 1106 94-95 2.9463475788775533 1106 96-97 3.6872418240399085 1106 98-99 4.149888960513469 1106 100-101 4.275069334103311 1107 1 0.19392919447173185 1107 2 0.2082945927676505 1107 3 0.17536755464982434 1107 4 0.17027642740909954 1107 5 0.08543051165607807 1107 6 0.14137204495162337 1107 7 0.15357911228520038 1107 8 0.18661830578636796 1107 9 0.27875616628670485 1107 10-11 0.28703219383793055 1107 12-13 0.2568113066435771 1107 14-15 0.38200339741349865 1107 16-17 0.4313608608199999 1107 18-19 0.42861766421467706 1107 20-21 0.3360293895996094 1107 22-23 0.32399936806262986 1107 24-25 0.39046887386501083 1107 26-27 0.3432850814945141 1107 28-29 0.2984139279171174 1107 30-31 0.22810647484191549 1107 32-33 0.33316935542168835 1107 34-35 0.4805186527726377 1107 36-37 0.4332938961874433 1107 38-39 0.46117130132493855 1107 40-41 0.5436549187131519 1107 42-43 0.5973516694715642 1107 44-45 0.7124560408593723 1107 46-47 0.6212783921389118 1107 48-49 0.5522308908168654 1107 50-51 0.5962547272002539 1107 52-53 0.7158599057474575 1107 54-55 0.6517222468691557 1107 56-57 0.5443864166405561 1107 58-59 0.4271361234958704 1107 60-61 0.7135712565546797 1107 62-63 0.9778727470434454 1107 64-65 0.9610663745579906 1107 66-67 1.160968541839246 1107 68-69 1.1597966958432373 1107 70-71 1.257362369325005 1107 72-73 1.4166455674879828 1107 74-75 1.285336783038364 1107 76-77 1.151304230173043 1107 78-79 1.4202432450005666 1107 80-81 1.6044752108699392 1107 82-83 1.730149745323935 1107 84-85 1.8943995775725853 1107 86-87 1.9442899680247514 1107 88-89 2.1242079822094126 1107 90-91 2.272482977789558 1107 92-93 2.4333596610836317 1107 94-95 2.5757231653998325 1107 96-97 2.8574500077044753 1107 98-99 3.3064698652665783 1107 100-101 3.814527325966356 1108 1 0.14071044958629741 1108 2 0.1854444379928708 1108 3 0.17431288727296845 1108 4 0.13362397706175955 1108 5 0.039352687406768894 1108 6 0.04946293666507984 1108 7 0.04608757981706191 1108 8 0.10200399754197065 1108 9 0.2024784154164223 1108 10-11 0.1319987963847069 1108 12-13 0.2374366608889602 1108 14-15 0.40291454673648985 1108 16-17 0.3513710555909384 1108 18-19 0.30011513780218024 1108 20-21 0.27046010451208957 1108 22-23 0.23170014737124234 1108 24-25 0.14934494617958904 1108 26-27 0.3502544234695222 1108 28-29 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2316 50-51 -0.2188779118948858 2316 52-53 -0.295484656172583 2316 54-55 -0.014025203985120527 2316 56-57 -0.11561060169705684 2316 58-59 -0.12248279741553603 2316 60-61 0.21124300362540538 2316 62-63 0.3190774674364967 2316 64-65 0.2766997299936058 2316 66-67 0.5474821394295617 2316 68-69 0.44199923501755833 2316 70-71 0.4484278023743933 2316 72-73 0.4443733690310303 2316 74-75 0.707099757270008 2316 76-77 0.6215174619633395 2316 78-79 0.7143856053297846 2316 80-81 0.933084359031632 2316 82-83 1.1828077568439213 2316 84-85 1.4074232759345051 2316 86-87 1.241269200590125 2316 88-89 1.6023874041337614 2316 90-91 1.7533110642686935 2316 92-93 1.7958027743606557 2316 94-95 2.410942705903807 2316 96-97 2.6211589225237653 2316 98-99 2.6082119522507234 2316 100-101 3.1679201767673284 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 10.0 4 29.0 5 81.0 6 171.0 7 489.0 8 1167.0 9 2017.0 10 3277.0 11 5100.0 12 7297.0 13 10040.0 14 12820.0 15 17133.0 16 21693.0 17 27456.0 18 25824.0 19 21216.0 20 20625.0 21 21868.0 22 23713.0 23 26535.0 24 30656.0 25 35385.0 26 41950.0 27 49105.0 28 58693.0 29 69818.0 30 82626.0 31 97027.0 32 115686.0 33 141172.0 34 176443.0 35 226396.0 36 278001.0 37 296379.0 38 148494.0 39 8447.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02012074059681 19.2013371068531 15.329504385837803 22.44903776671229 2 16.121749413478028 20.899431919723487 38.53501640740558 24.443802259392907 3 19.347998876875852 24.61642032208578 30.093693448870056 25.941887352168308 4 13.398462405325615 17.11721427600879 35.852266626632066 33.63205669203352 5 13.640377930797024 37.84857943047862 33.50043491432259 15.010607724401762 6 32.312862793762584 36.22673582770426 16.60777082978992 14.852630548743234 7 28.936938854434008 33.2459001751197 19.801543675879376 18.01561729456692 8 27.530945256802635 34.1775122740571 18.95430829618889 19.33723417295137 9 27.005538647482997 16.385521791142914 19.557297778554823 37.05164178281927 10-11 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29.111080595131295 21.97746875332614 36-37 22.84917461878529 25.49450418926399 29.239993633554022 22.416327558396702 38-39 22.145081526335698 26.04692301990501 29.242025547896127 22.565969905863167 40-41 22.478346733070257 25.947311868572587 29.39848354080892 22.17585785754824 42-43 22.280297652815033 25.989626242556067 28.887593396600238 22.842482708028662 44-45 22.226727803203826 26.11990610020824 29.28313276767308 22.370233328914853 46-47 22.962818147093085 26.588288728285804 28.21577276035659 22.233120364264526 48-49 22.525115851706015 26.852582089140963 27.84985219821308 22.772449860939943 50-51 22.65574298547257 26.90372597330161 28.003735190127927 22.436795851097894 52-53 23.130837125376395 26.559686551890255 27.88669574876262 22.422780573970734 54-55 22.38025964844934 26.911636290731007 27.998604173416965 22.70949988740269 56-57 22.54718397450455 26.98565595772802 27.756306162066014 22.710853905701413 58-59 22.536850676961638 26.46861100527069 27.824909755868127 23.16962856189954 60-61 22.026932136602866 26.935082186535265 28.172417364500774 22.865568312361095 62-63 22.213715397706054 26.897074655342955 28.38381050205098 22.505399444900007 64-65 21.97569323361424 27.01743975568759 28.01136045107338 22.99550655962479 66-67 21.86011023149945 27.009401651334564 27.891890274001437 23.23859784316455 68-69 22.506830682222553 26.53152898484969 28.055230775151184 22.906409557776573 70-71 22.31106218697235 26.824955364905556 27.524674014155902 23.339308433966192 72-73 22.265690696611536 26.956271385174972 27.56648229846744 23.21155561974605 74-75 22.23730382175228 27.491227386706672 27.24524739577147 23.02622139576957 76-77 22.266379583114393 26.868735289897693 27.756971293861877 23.107913833126034 78-79 22.33032725434545 27.123979616561023 27.669910292724502 22.875782836369027 80-81 22.691285417385743 27.158857977937096 27.21461028849861 22.93524631617855 82-83 22.466298355140708 27.193187289902916 27.420474000162958 22.920040354793414 84-85 22.211107180857827 27.454305262535243 27.362109280626655 22.972478275980272 86-87 22.61674366078341 27.358016585309535 27.026277137486467 22.99896261642059 88-89 22.273529749919945 27.52607554186862 26.686346649588614 23.514048058622816 90-91 22.18938502590591 27.379594109448824 26.99927808845234 23.43174277619293 92-93 22.40244731539782 27.082287131972688 27.155713245253672 23.359552307375818 94-95 22.10966978106712 26.711004035449626 27.436520296496752 23.742805886986506 96-97 22.320777862133383 26.32149810484948 27.458920985193213 23.89880304782393 98-99 22.04790754287962 26.864910782071465 26.933514375873578 24.153667299175332 100-101 22.185111817792453 26.31727352867606 27.414487769917816 24.083126883613666 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2057.0 1 1319.0 2 849.5 3 1130.0 4 928.5 5 813.0 6 1456.0 7 3161.5 8 4836.5 9 5152.0 10 4025.0 11 2875.0 12 2714.5 13 2778.0 14 2817.5 15 2957.0 16 3079.5 17 3226.5 18 3439.5 19 3712.0 20 4065.5 21 4670.0 22 5492.0 23 6469.5 24 7782.5 25 9548.0 26 12139.5 27 14913.0 28 16954.0 29 19362.5 30 22700.5 31 27624.0 32 35414.5 33 38433.5 34 37200.0 35 38782.5 36 42939.5 37 47412.0 38 50771.5 39 53574.0 40 55353.5 41 58535.5 42 60826.0 43 62057.5 44 64107.5 45 68083.0 46 70191.5 47 72781.0 48 77209.5 49 75973.5 50 73443.5 51 72256.5 52 72095.0 53 74785.5 54 77564.0 55 74967.5 56 70174.0 57 64812.5 58 59675.5 59 53402.0 60 43803.0 61 36883.0 62 31573.0 63 23198.0 64 15472.5 65 11340.0 66 8741.5 67 6898.5 68 5258.5 69 4001.5 70 3023.5 71 2102.0 72 1517.0 73 1185.0 74 886.5 75 666.5 76 476.5 77 342.0 78 250.5 79 145.0 80 90.5 81 66.5 82 37.5 83 16.5 84 13.0 85 8.5 86 3.0 87 1.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.4252824197114657E-4 2 0.04361364204317086 3 1.4252824197114657E-4 4 0.0 5 0.10133758004148521 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.026788658172617E-4 26-27 0.010499580491874464 28-29 0.0 30-31 0.0 32-33 4.5133943290863086E-4 34-35 0.014347843025095421 36-37 0.002898074253413314 38-39 0.011877353497595548 40-41 0.006675072665648698 42-43 0.005321054366922806 44-45 0.0023279612855287278 46-47 9.501882798076439E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.4252824197114657E-4 68-69 2.3754706995191097E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.3754706995191096E-5 82-83 7.36395916850924E-4 84-85 0.004299601966129589 86-87 0.001496546540697039 88-89 0.0 90-91 8.314147448316885E-4 92-93 4.275847259134397E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.36395916850924E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2104846.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.91335276308493 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6390930079513 31.01999410122532 2 14.767534667572477 12.969839185963927 3 5.514068824229669 7.264237483149792 4 2.743709363805576 4.819419086886943 5 1.56031625803106 3.4259359130447296 6 1.0059359270543926 2.6504411533080243 7 0.6866306383241358 2.110657740706897 8 0.5062286230990045 1.778415688393388 9 0.36912568321072936 1.4588591706672747 >10 1.988919203279565 16.016370227470027 >50 0.13951767990674574 4.169201924539974 >100 0.0687320613609664 5.664623680403445 >500 0.004985647446982759 1.544406593311189 >1k 0.004877263806830958 3.793312751915366 >5k 2.1676728030359807E-4 0.7772245575756328 >10k+ 1.0838364015179904E-4 0.5370607414380552 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11284 0.5360962274674727 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9435 0.448251320999256 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6895 0.3275774094636852 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3464 0.16457261006268392 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3219 0.15293280363504028 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3200 0.152030124769223 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2937 0.13953514888975252 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2849 0.13535432045859888 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2784 0.13226620854922402 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2684 0.12751526715018582 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2361 0.11216972643129236 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2263 0.1075138038602349 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2211 0.10504331433273503 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2198 0.10442569195086007 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2125 0.10095750472956216 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.750941399038219E-5 2 0.0 0.0 0.0 0.0 4.750941399038219E-5 3 0.0 0.0 0.0 0.0 4.750941399038219E-5 4 0.0 0.0 0.0 0.0 4.750941399038219E-5 5 0.0 0.0 0.0 0.0 4.750941399038219E-5 6 0.0 0.0 0.0 0.0 4.750941399038219E-5 7 0.0 0.0 0.0 0.0 4.750941399038219E-5 8 0.0 0.0 0.0 0.0 4.750941399038219E-5 9 0.0 0.0 0.0 0.0 4.750941399038219E-5 10-11 0.0 0.0 0.0 2.3754706995191096E-5 4.750941399038219E-5 12-13 0.0 0.0 0.0 4.750941399038219E-5 5.463582608893952E-4 14-15 0.0 0.0 0.0 4.750941399038219E-5 6.413770888701597E-4 16-17 0.0 0.0 0.0 7.126412098557329E-5 7.126412098557329E-4 18-19 0.0 0.0 0.0 1.4252824197114657E-4 7.36395916850924E-4 20-21 0.0 0.0 0.0 2.3754706995191097E-4 8.314147448316885E-4 22-23 0.0 0.0 0.0 7.126412098557329E-4 8.551694518268795E-4 24-25 0.0 0.0 0.0 0.001449037126706657 8.551694518268795E-4 26-27 0.0 0.0 0.0 0.002803055425432549 8.551694518268795E-4 28-29 0.0 0.0 0.0 0.006793846200624654 8.551694518268795E-4 30-31 0.0 0.0 0.0 0.01736469081348469 8.551694518268795E-4 32-33 0.0 0.0 0.0 0.03632094699564718 8.551694518268795E-4 34-35 0.0 0.0 0.0 0.06439901066396306 9.026788658172617E-4 36-37 0.0 0.0 0.0 0.11437891418184513 9.026788658172617E-4 38-39 0.0 0.0 0.0 0.1897288447705913 9.026788658172617E-4 40-41 0.0 0.0 0.0 0.2675255101798421 9.026788658172617E-4 42-43 0.0 0.0 0.0 0.3536363230374099 9.026788658172617E-4 44-45 0.0 0.0 0.0 0.457824467918318 9.501882798076439E-4 46-47 0.0 0.0 0.0 0.5778807570720138 9.97697693798026E-4 48-49 0.0 0.0 0.0 0.7055860618781612 9.97697693798026E-4 50-51 0.0 0.0 0.0 0.8527464717133699 0.0010452071077884082 52-53 0.0 0.0 0.0 0.9930180165199735 0.0011402259357691726 54-55 0.0 0.0 0.0 1.1439079153534273 0.0011402259357691726 56-57 0.0 0.0 0.0 1.315416899858707 0.0011639806427643637 58-59 0.0 0.0 0.0 1.4997534261413898 0.001187735349759555 60-61 0.0 0.0 0.0 1.706229339343591 0.001187735349759555 62-63 0.0 0.0 0.0 1.9200929664212962 0.001187735349759555 64-65 0.0 0.0 0.0 2.1300133121378 0.001187735349759555 66-67 0.0 0.0 0.0 2.3757795107100472 0.001187735349759555 68-69 0.0 0.0 0.0 2.651334111854264 0.001187735349759555 70-71 0.0 0.0 0.0 2.942923140220235 0.001187735349759555 72-73 0.0 0.0 0.0 3.2468408615167093 0.0012589994707451282 74-75 0.0 0.0 0.0 3.5510436392971267 0.0013777730057210837 76-77 0.0 0.0 0.0 3.8783597469838647 0.0015678106616826123 78-79 0.0 0.0 4.750941399038219E-5 4.2573185876781485 0.0015678106616826123 80-81 0.0 0.0 4.750941399038219E-5 4.657537891133128 0.0015678106616826123 82-83 0.0 0.0 4.750941399038219E-5 5.0690881898248135 0.0016390747826681858 84-85 0.0 0.0 4.750941399038219E-5 5.49363231324287 0.0017340936106489502 86-87 0.0 0.0 4.750941399038219E-5 5.922974887473953 0.0017816030246393323 88-89 0.0 0.0 4.750941399038219E-5 6.378803960004674 0.0018053577316345234 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7390 0.0 39.777264 1 GTATCAA 11755 0.0 36.01291 1 AGGTAAG 1170 0.0 31.302958 2 TATCAAC 14970 0.0 28.15091 2 ATCAACG 15335 0.0 27.43745 3 TCAACGC 15360 0.0 27.392792 4 CGCGAAA 35 0.008399083 27.139532 72-73 CAACGCA 15660 0.0 26.989466 5 AACGCAG 16105 0.0 26.216908 6 TAAGGTA 710 0.0 26.115028 4 TAAGGTG 1470 0.0 25.873602 5 AAGGTAA 1435 0.0 24.528479 1 GTGTAAG 920 0.0 24.299698 1 GTAAGGT 1535 0.0 24.158527 4 GTACATG 10720 0.0 23.915794 1 TACATGG 10715 0.0 23.526875 2 ACGCAGA 18145 0.0 23.086176 7 CGCAGAG 18170 0.0 22.949856 8 ACATGGG 10625 0.0 22.686277 3 GGTAAGG 1630 0.0 22.458843 3 >>END_MODULE