FastQCFastQC Report
Thu 26 May 2016
SRR938512_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938512_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105770
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108300.5143011819904358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85340.40526743186577835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63210.3001752328127003No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32520.15443282029851313No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA32090.15239081191203216No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA29360.1394264330862345No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA28420.13496250777625288No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27910.13254059085275222No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27650.13130588810743815No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24460.1161570351937771No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG24070.114304981075806No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA22710.1078465359464709No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA22550.10708671887243147No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.10119813654862592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70100.039.6095921
GTATCAA113700.037.2184751
AGGTAAG12550.034.4602052
TAAGGTG14500.031.7924445
GGTAAGG14650.030.493723
AAGGTAA14250.030.1316591
GTAAGGT15350.029.4127354
TATCAAC144400.029.0612092
ATCAACG145700.028.834533
TCAACGC147400.028.5342184
CAACGCA149750.027.9912285
AACGCAG154400.027.2347436
GTACATG99150.025.550371
TACATGG97650.025.5022932
ACGCAGA171450.024.300617
CGCAGAG172850.024.1037888
ACATGGG101700.023.5053753
GAGTACT97700.022.48643912-13
AGGTGTG24250.022.1371547
GCAGAGT182450.021.8445079
Produced by FastQC (version 0.11.5)