FastQCFastQC Report
Thu 26 May 2016
SRR938512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938512_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105770
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107500.5105020966202386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92710.44026650583871935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67940.32263732506399084No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA32570.15467026313415044No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA32440.1540529117614934No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC31920.15158350627086528No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA29160.1384766617436852No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29150.13842917317655773No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG26770.12712689420022127No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26690.12674698566320158No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA22610.10737165027519624No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA22080.10485475621744066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22070.10480726765031319No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21420.10172051078702801No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA21140.10039083090745904No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT21070.10005841093756677No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78450.036.2174571
GTATCAA120750.034.036011
GTAAGGT12500.027.3588664
TAAGGTG14200.027.0939485
AGGTAAG12500.026.9808032
TATCAAC152000.026.8132952
ATCAACG154500.026.2853183
TCAACGC155950.026.0409184
CAACGCA158250.025.6624415
AACGCAG162450.025.057446
GGTAAGG14500.024.5679453
GTACATG105150.024.1291281
TACATGG103400.024.0722832
AAGGTAA14700.022.3018781
ACATGGG104900.022.2321573
ACGCAGA184850.021.8668237
CGCAGAG185850.021.7491658
GAGTACT100650.021.33046212-13
AGTACTT105500.021.11523612-13
AGAGTAC155350.021.03549810-11
Produced by FastQC (version 0.11.5)