##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938512_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2105770 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.798697388603692 31.0 31.0 34.0 30.0 34.0 2 32.04449631251276 31.0 31.0 34.0 30.0 34.0 3 32.089397227617454 33.0 31.0 34.0 30.0 34.0 4 35.757757019997435 37.0 35.0 37.0 35.0 37.0 5 35.650770027115975 37.0 35.0 37.0 33.0 37.0 6 35.692897135014746 37.0 35.0 37.0 33.0 37.0 7 35.61436101758502 37.0 35.0 37.0 33.0 37.0 8 35.58200088328735 37.0 35.0 37.0 33.0 37.0 9 37.11311254315523 39.0 37.0 39.0 33.0 39.0 10-11 37.04531121632467 39.0 37.0 39.0 33.0 39.0 12-13 36.92729832792755 39.0 37.0 39.0 33.0 39.0 14-15 37.901342976678364 40.0 37.0 41.0 32.5 41.0 16-17 37.98676612355575 40.0 37.0 41.0 33.0 41.0 18-19 38.152910336836406 40.0 37.0 41.0 33.0 41.0 20-21 38.11483732791331 40.0 37.0 41.0 33.0 41.0 22-23 37.99016013144835 40.0 37.0 41.0 33.0 41.0 24-25 37.85433974270694 40.0 37.0 41.0 32.5 41.0 26-27 37.62389885884973 40.0 37.0 41.0 32.0 41.0 28-29 37.47567944267418 39.5 36.5 41.0 32.0 41.0 30-31 37.17638298579617 39.0 36.0 41.0 31.0 41.0 32-33 37.01336636954653 39.0 36.0 41.0 31.0 41.0 34-35 36.85605170555189 39.0 35.0 41.0 30.5 41.0 36-37 36.70191473902658 39.0 35.0 41.0 30.0 41.0 38-39 36.46413022314877 39.0 35.0 40.0 30.0 41.0 40-41 36.214187209429326 38.5 35.0 40.0 30.0 41.0 42-43 35.910938516552136 38.0 35.0 40.0 29.0 41.0 44-45 35.56977993797993 38.0 34.5 40.0 27.5 41.0 46-47 35.08199091068825 38.0 34.0 40.0 26.5 41.0 48-49 34.60685117557948 38.0 33.5 40.0 24.5 41.0 50-51 34.171334001339176 38.0 33.0 40.0 23.5 41.0 52-53 33.54993066669199 37.0 32.5 40.0 22.5 41.0 54-55 33.11454551066831 37.0 32.0 39.5 20.5 41.0 56-57 32.70059788106013 36.0 31.0 39.0 19.0 40.0 58-59 32.152813460159464 35.5 31.0 39.0 16.5 40.0 60-61 31.651508949220474 35.0 30.0 39.0 13.5 40.0 62-63 31.152667907701222 35.0 29.5 38.0 8.5 40.0 64-65 30.49830798235325 34.5 29.0 38.0 7.5 40.0 66-67 29.850985387767892 34.0 28.0 37.0 4.0 39.5 68-69 29.1279835879512 34.0 27.0 36.5 2.0 39.0 70-71 28.353016473783935 33.0 26.0 36.0 2.0 39.0 72-73 27.780689961391793 33.0 25.5 35.0 2.0 38.0 74-75 27.09262407575376 32.0 24.5 35.0 2.0 37.5 76-77 25.973075881981416 30.5 23.0 34.0 2.0 36.0 78-79 26.47760534151403 32.0 24.0 34.5 2.0 36.0 80-81 26.12062950844584 32.0 23.0 35.0 2.0 36.0 82-83 25.469941399108166 31.0 20.5 34.0 2.0 35.5 84-85 24.717771409033276 31.0 18.5 34.0 2.0 35.0 86-87 23.856511869767353 30.5 11.0 34.0 2.0 35.0 88-89 22.894744915161674 29.5 4.5 34.0 2.0 35.0 90-91 22.053803834226912 29.0 2.0 34.0 2.0 35.0 92-93 21.01397564786278 28.5 2.0 33.5 2.0 35.0 94-95 19.871632466983577 26.5 2.0 33.0 2.0 34.5 96-97 18.52777297615599 25.0 2.0 32.5 2.0 34.0 98-99 16.79562155411085 12.5 2.0 32.0 2.0 34.0 100-101 12.605396125882693 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17694968300790848 1101 2 0.15010398091172306 1101 3 0.22042267356074063 1101 4 0.10122424379849093 1101 5 0.10919867452646059 1101 6 0.07057852749976945 1101 7 0.12505978332374923 1101 8 0.0934900356061874 1101 9 0.15448952529347082 1101 10-11 0.1478833549642644 1101 12-13 0.2158864719772211 1101 14-15 0.24582261097384617 1101 16-17 0.24008385721984382 1101 18-19 0.233208461353982 1101 20-21 0.18808241632485334 1101 22-23 0.28126340094507896 1101 24-25 0.06729951980015159 1101 26-27 0.13935047854945282 1101 28-29 0.1610559147461501 1101 30-31 0.27993337415507114 1101 32-33 0.22812118833051898 1101 34-35 -0.009017132749413292 1101 36-37 0.16423560576922824 1101 38-39 0.3402141574117721 1101 40-41 0.5647425631255132 1101 42-43 0.5657084464732449 1101 44-45 0.5329380385602747 1101 46-47 0.44240681761499445 1101 48-49 0.35890810204160317 1101 50-51 0.49820430451293163 1101 52-53 0.7491679228661638 1101 54-55 0.6809240774370764 1101 56-57 0.8672694205750489 1101 58-59 0.8249176339620305 1101 60-61 0.8342172575659497 1101 62-63 0.9953043917552371 1101 64-65 1.1073174965719481 1101 66-67 1.2765041477829833 1101 68-69 1.1194593702495936 1101 70-71 1.3146453671991303 1101 72-73 1.5025508286323088 1101 74-75 1.441431545886104 1101 76-77 1.5623259963665994 1101 78-79 1.6593887122618582 1101 80-81 1.6888510673057233 1101 82-83 1.9236617592907237 1101 84-85 1.940435513254645 1101 86-87 2.061211872504991 1101 88-89 1.750795296198735 1101 90-91 2.0899220580255893 1101 92-93 2.3390865892736272 1101 94-95 2.3199336422297776 1101 96-97 2.065470583260005 1101 98-99 2.1470372931900314 1101 100-101 1.8783937045874701 1102 1 0.201425233175069 1102 2 0.2067286426063717 1102 3 0.23917428089385595 1102 4 0.22707995733193087 1102 5 0.09288653962069304 1102 6 0.19015431402144145 1102 7 0.1835385420339648 1102 8 0.25788745407612623 1102 9 0.2981048920103433 1102 10-11 0.34790417375506166 1102 12-13 0.3886138560349295 1102 14-15 0.5506195053001193 1102 16-17 0.36109981421067516 1102 18-19 0.3760621052546895 1102 20-21 0.36815271035965935 1102 22-23 0.3228936347278548 1102 24-25 0.2673583022682777 1102 26-27 0.28246864355673296 1102 28-29 0.2762585305659897 1102 30-31 0.3490542088661286 1102 32-33 0.23661525497514901 1102 34-35 0.44837415030041683 1102 36-37 0.5050686023280022 1102 38-39 0.535783428876563 1102 40-41 0.5527325701059382 1102 42-43 0.7817511617334176 1102 44-45 0.6547953196261957 1102 46-47 0.7986530671710597 1102 48-49 1.0036587051742174 1102 50-51 0.957906473341076 1102 52-53 0.9839108107998413 1102 54-55 0.8503902591483836 1102 56-57 1.0985747587579269 1102 58-59 1.2359099555021302 1102 60-61 1.098177481429179 1102 62-63 1.191559458681521 1102 64-65 1.3057352684714836 1102 66-67 1.2943189095302934 1102 68-69 1.4494127116655164 1102 70-71 1.5356686719984758 1102 72-73 1.3898991270023195 1102 74-75 1.4874863850303264 1102 76-77 1.4024536033078299 1102 78-79 1.6456544335104937 1102 80-81 1.803715683933813 1102 82-83 1.9234584699217763 1102 84-85 1.896052300547833 1102 86-87 1.7730969527797988 1102 88-89 1.7542365198537802 1102 90-91 1.8641753369851415 1102 92-93 2.068716627114842 1102 94-95 1.8052747642401386 1102 96-97 1.8534777351269618 1102 98-99 2.3251065340389445 1102 100-101 1.8956145184797268 1103 1 0.17055335848008824 1103 2 0.20113481538879796 1103 3 0.2274932045611493 1103 4 0.12220918198401876 1103 5 0.1571508247111808 1103 6 0.1825572606825574 1103 7 0.19550755483057003 1103 8 0.18587071728755689 1103 9 0.2306743332440817 1103 10-11 0.2873390117554848 1103 12-13 0.3803401134773523 1103 14-15 0.5320961493120109 1103 16-17 0.4584092393138022 1103 18-19 0.44729427297189517 1103 20-21 0.40761618789239407 1103 22-23 0.3837548241802864 1103 24-25 0.44559713798781786 1103 26-27 0.44496819405734556 1103 28-29 0.4368888057623579 1103 30-31 0.48537594254673166 1103 32-33 0.7035087261891988 1103 34-35 0.6628805229174262 1103 36-37 0.7617317243709394 1103 38-39 0.5164925847937667 1103 40-41 0.5218948573642948 1103 42-43 0.5129116150342838 1103 44-45 0.7615000524694437 1103 46-47 0.6622961441099449 1103 48-49 0.6916607959885113 1103 50-51 0.6708583486525228 1103 52-53 0.8633951451040929 1103 54-55 0.81507925694455 1103 56-57 1.0933928014064307 1103 58-59 1.2068693413061737 1103 60-61 1.1684786717035571 1103 62-63 1.1825165584601187 1103 64-65 1.1465272093327705 1103 66-67 1.0455673619681214 1103 68-69 0.9796447560914672 1103 70-71 1.1547208961214395 1103 72-73 1.4382718107961985 1103 74-75 1.212880917062936 1103 76-77 1.2516014809001597 1103 78-79 1.4037756031335817 1103 80-81 1.711399426440014 1103 82-83 1.7024687750162748 1103 84-85 1.8659710310588267 1103 86-87 1.9207937179253598 1103 88-89 1.750732351068045 1103 90-91 1.9169646733185601 1103 92-93 2.001090343473024 1103 94-95 1.6676772228733547 1103 96-97 1.7342800016312943 1103 98-99 1.3287902457223346 1103 100-101 1.1993625727895267 1104 1 0.14858680906361954 1104 2 0.21565623471161643 1104 3 0.1465666705162718 1104 4 0.10566179317457625 1104 5 0.10263889676453886 1104 6 0.0886652943648727 1104 7 0.1317111202278909 1104 8 0.14078161132111688 1104 9 0.14724919200796194 1104 10-11 0.21555975528846005 1104 12-13 0.24762245633134938 1104 14-15 0.2894672939835701 1104 16-17 0.33077702386439256 1104 18-19 0.24010890050538336 1104 20-21 0.19007587368246703 1104 22-23 0.16765269967621776 1104 24-25 0.20687913034643657 1104 26-27 0.3371458469356128 1104 28-29 0.16260372460288153 1104 30-31 0.2764501625979605 1104 32-33 0.5064084236817621 1104 34-35 0.4086389736699303 1104 36-37 0.4034549643386143 1104 38-39 0.5221288739070715 1104 40-41 0.449283415766196 1104 42-43 0.4499406066986751 1104 44-45 0.5465505556668688 1104 46-47 0.7042091874622685 1104 48-49 0.9038704182935362 1104 50-51 0.8728439822042944 1104 52-53 0.7492002754680982 1104 54-55 1.0014239136280523 1104 56-57 1.0294588963375801 1104 58-59 0.8215883849848495 1104 60-61 0.8256381915152353 1104 62-63 1.0252348963202813 1104 64-65 0.9125107157734895 1104 66-67 1.0368712385628491 1104 68-69 1.191117058749537 1104 70-71 1.3266093082422081 1104 72-73 1.5298163995048384 1104 74-75 1.5071670458463338 1104 76-77 1.4781630681517797 1104 78-79 1.582839514951651 1104 80-81 1.6740392021615982 1104 82-83 1.6982969869008713 1104 84-85 2.0496168492048312 1104 86-87 1.902904453222174 1104 88-89 1.9082531080221905 1104 90-91 2.130001049524658 1104 92-93 2.152041363916659 1104 94-95 2.018163788665582 1104 96-97 2.1142404043437253 1104 98-99 2.302374794766102 1104 100-101 1.861308333871408 1105 1 0.21823456979042888 1105 2 0.21950893088538947 1105 3 0.17034368461919058 1105 4 0.16309891467790294 1105 5 0.14400646810202034 1105 6 0.10238474129650399 1105 7 0.19457005188615284 1105 8 0.15136260011382063 1105 9 0.1477491356146885 1105 10-11 0.27218561931392316 1105 12-13 0.33018347039745066 1105 14-15 0.36764988848833013 1105 16-17 0.2816583701213773 1105 18-19 0.3998224687262848 1105 20-21 0.42515455955813053 1105 22-23 0.39893059420469257 1105 24-25 0.33833690789914783 1105 26-27 0.4263173031939971 1105 28-29 0.44128234971192626 1105 30-31 0.4367527527753978 1105 32-33 0.3913650008634306 1105 34-35 0.50454263554969 1105 36-37 0.5431192287286777 1105 38-39 0.48863437858080516 1105 40-41 0.43751770793857503 1105 42-43 0.4084151342036293 1105 44-45 0.3247758479336298 1105 46-47 0.3846395716960842 1105 48-49 0.35693337850290163 1105 50-51 0.3561821876092992 1105 52-53 0.503301677473857 1105 54-55 0.4493285008178063 1105 56-57 0.7074221325287056 1105 58-59 0.7014315886750353 1105 60-61 0.7014368468234586 1105 62-63 0.8263997050780425 1105 64-65 1.218718233802072 1105 66-67 1.008678976640276 1105 68-69 1.2443145571900907 1105 70-71 1.3949244614592686 1105 72-73 1.6196124400067156 1105 74-75 1.540431019293159 1105 76-77 1.5561648589258432 1105 78-79 1.7435645943050382 1105 80-81 1.8682349535616432 1105 82-83 1.9622873517077863 1105 84-85 2.0311411477991435 1105 86-87 2.152681066817788 1105 88-89 2.0844709149454452 1105 90-91 2.1897377504953113 1105 92-93 2.2166010705795784 1105 94-95 1.998159024009663 1105 96-97 2.2320582609851236 1105 98-99 2.300302169440691 1105 100-101 2.3232330937396544 1106 1 -0.0544082630141034 1106 2 -0.03531204963952206 1106 3 0.010301026165606686 1106 4 -0.02081920233193557 1106 5 -0.002872694305438017 1106 6 -0.0804208928054777 1106 7 -0.04729983983040853 1106 8 -0.12407029026191907 1106 9 -0.28351411218047673 1106 10-11 -0.06890146322841417 1106 12-13 -0.035497493931409 1106 14-15 -0.03801098565595851 1106 16-17 -0.12178837982662571 1106 18-19 -0.12739043855353316 1106 20-21 -0.08481995157332989 1106 22-23 0.022389867329415836 1106 24-25 -0.07356519891514068 1106 26-27 -0.23971015044148913 1106 28-29 -0.09486604329456583 1106 30-31 0.03789532670860751 1106 32-33 -0.11901087144441647 1106 34-35 -0.12148123371365926 1106 36-37 -0.10912808409850783 1106 38-39 0.08058724187462474 1106 40-41 0.10349582114277922 1106 42-43 0.1934105541725657 1106 44-45 0.16925148969112058 1106 46-47 -0.38601459834475804 1106 48-49 -0.15708218032726506 1106 50-51 -0.02097872414876889 1106 52-53 -0.06127299915825546 1106 54-55 -0.2505347620228804 1106 56-57 -0.040288073196556695 1106 58-59 0.17859338546326597 1106 60-61 0.3416404480111659 1106 62-63 0.07319257704515181 1106 64-65 0.3514043888328011 1106 66-67 0.38822592970270264 1106 68-69 0.3269071202816747 1106 70-71 0.0740831075401367 1106 72-73 0.4965414738635445 1106 74-75 0.48048792673228036 1106 76-77 0.4051092392056006 1106 78-79 0.8148453656973231 1106 80-81 0.9133610924848306 1106 82-83 0.9864977941786108 1106 84-85 1.0354634138303567 1106 86-87 0.822700670235772 1106 88-89 0.7604576129421794 1106 90-91 0.9133187152666835 1106 92-93 0.8727608804751021 1106 94-95 0.6888816953846941 1106 96-97 1.0316159671639618 1106 98-99 1.0882403146829844 1106 100-101 1.3432281375707742 1107 1 0.08926159751264962 1107 2 0.048157158939432065 1107 3 0.11932111543152502 1107 4 0.09727443946670888 1107 5 0.026481426356852467 1107 6 3.023055647801698E-4 1107 7 0.06868504349424853 1107 8 0.14427160099481284 1107 9 0.07643873372495591 1107 10-11 0.00557179056880841 1107 12-13 0.11026528879362019 1107 14-15 0.20222721726881332 1107 16-17 0.12223564317017122 1107 18-19 0.1082223049608757 1107 20-21 0.16536818338214943 1107 22-23 0.22901209107192955 1107 24-25 0.17644430285028534 1107 26-27 0.18673098232098795 1107 28-29 0.23189016800949247 1107 30-31 0.1725112716845203 1107 32-33 0.15354505277964137 1107 34-35 0.22743024403890644 1107 36-37 0.2389089145091674 1107 38-39 0.09199917887918474 1107 40-41 0.2325063579671891 1107 42-43 0.18965070559269748 1107 44-45 0.1665336680531908 1107 46-47 0.18675308855813455 1107 48-49 -0.0339906099134879 1107 50-51 0.169335936315143 1107 52-53 0.1871167683272148 1107 54-55 0.046119856405226756 1107 56-57 0.24575148962087923 1107 58-59 0.31112654345734825 1107 60-61 0.47975304266458707 1107 62-63 0.5435619550089115 1107 64-65 0.4096540116127194 1107 66-67 0.31408302061685944 1107 68-69 0.5656304250717099 1107 70-71 0.5679519350349516 1107 72-73 0.9365162878506474 1107 74-75 1.0266899444004984 1107 76-77 1.1039843568691303 1107 78-79 1.1721323329491717 1107 80-81 1.4148934105228541 1107 82-83 1.4722479965662139 1107 84-85 1.4613281061568593 1107 86-87 1.8557902516133247 1107 88-89 1.741467384557744 1107 90-91 1.9245643713762561 1107 92-93 2.0345768958907655 1107 94-95 1.7738024569955329 1107 96-97 2.039786851981031 1107 98-99 2.2786012170719836 1107 100-101 1.895637658593115 1108 1 0.09580567548952601 1108 2 0.1506368345376643 1108 3 0.2067451696530327 1108 4 0.0742838322768904 1108 5 -0.008294854362766557 1108 6 0.045736835807765885 1108 7 0.05003385366460833 1108 8 0.0392377454138213 1108 9 -0.03679657884394061 1108 10-11 0.1304532676555752 1108 12-13 0.05355741468989805 1108 14-15 0.16424939890297452 1108 16-17 0.06932826938700032 1108 18-19 0.12812638728732395 1108 20-21 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2316 98-99 1.16140518764972 2316 100-101 0.7481644980798894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 17.0 4 46.0 5 95.0 6 210.0 7 549.0 8 1275.0 9 2163.0 10 3677.0 11 5585.0 12 7952.0 13 10914.0 14 14567.0 15 19431.0 16 24552.0 17 30894.0 18 30366.0 19 26439.0 20 26256.0 21 28773.0 22 31400.0 23 34757.0 24 39221.0 25 44553.0 26 51429.0 27 59549.0 28 70343.0 29 81869.0 30 95524.0 31 111587.0 32 131736.0 33 157462.0 34 191573.0 35 231726.0 36 257144.0 37 215601.0 38 65203.0 39 1324.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.07746823081012 19.20163894012292 15.334426150081962 22.386466678985002 2 16.19776305478379 20.828762081166442 38.60706228776329 24.366412576286482 3 19.376380453607972 24.60490117721142 30.063507395740878 25.95521097343973 4 13.427772263827483 17.133305156783504 35.84949923305964 33.58942334632937 5 13.661860800851 37.78721221791657 33.55002469417217 15.000902287060253 6 32.310556233586766 36.25837579602711 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22.037053482175377 26.55585826921972 29.544033124272534 21.86305512433237 30-31 22.051316145637937 26.00300602629917 29.341143619673566 22.604534208389328 32-33 21.76176088234072 26.128753836199476 29.356753195101536 22.752732086358268 34-35 22.732322162517466 26.165778377674958 29.103187486761158 21.998711973046422 36-37 22.889899565046225 25.502542810345624 29.244669691096863 22.362887933511292 38-39 22.196297969169215 26.066389201958877 29.154454562423147 22.58285826644876 40-41 22.520803297846044 25.969160228554276 29.28633663667293 22.223699836926748 42-43 22.306692986211683 25.987368802980733 28.85001112478062 22.855927086026966 44-45 22.244903483411154 26.157591207321662 29.182876236137968 22.41462907312922 46-47 22.998415305762407 26.59237551697266 28.157908108629197 22.251301068635733 48-49 22.50604292016697 26.811926278748395 27.89364460506133 22.788386196023307 50-51 22.640957939376094 26.880024884009174 28.041001628857853 22.43801554775688 52-53 23.115131282143825 26.529701724309874 27.88008661914644 22.475080374399862 54-55 22.4300137241959 26.90766323007736 27.93135052736054 22.7309725183662 56-57 22.58017257345294 26.989462286954414 27.662470260284838 22.767894879307807 58-59 22.54989861190918 26.463312707465676 27.730925029799074 23.255863650826065 60-61 22.024342639509538 26.997582831933215 28.124676484136447 22.853398044420807 62-63 22.224364484250415 26.898996566576592 28.353096492019546 22.52354245715344 64-65 21.95191446733105 26.989386535129572 28.015805156169826 23.04289384136955 66-67 21.88193040225895 26.93488520523251 27.923080429944942 23.2601039625636 68-69 22.550514116598947 26.50998600022415 27.987394503045948 22.952105380130956 70-71 22.33623441127683 26.758538092259883 27.57379845557609 23.3314290408872 72-73 22.279155832329938 26.892131677467564 27.556545324469734 23.272167165732764 74-75 22.253807395869444 27.439772624740595 27.20472796174321 23.10169201764675 76-77 22.317727007223013 26.827075131662053 27.75519168760121 23.100006173513727 78-79 22.325016502277077 27.147551726921744 27.556024637068628 22.971407133732555 80-81 22.667965954480287 27.209489739852756 27.1384468096928 22.984097495974154 82-83 22.490040323097666 27.20477019728447 27.306278407458468 22.998911072159398 84-85 22.21742821354578 27.418394666698326 27.285893227110552 23.078283892645338 86-87 22.628457559472587 27.328373405975086 26.912663676653008 23.130505357899317 88-89 22.263138899309993 27.432934271074238 26.638569264449586 23.665357565166186 90-91 22.18942835460687 27.299327649203654 26.960112948095354 23.55113104809412 92-93 22.421578008837688 26.9967303888629 27.042699649294434 23.538991953004977 94-95 22.088457449877584 26.648476958185597 27.317329840706687 23.94573575123013 96-97 22.362234414126146 26.32316270208176 27.288439520194284 24.02616336359781 98-99 22.07259102371104 26.81031166746606 26.72858384343969 24.38851346538321 100-101 22.38148040859163 26.19355390189812 27.138885063420982 24.28608062608927 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1873.0 1 1293.5 2 881.5 3 1073.0 4 928.5 5 838.0 6 1366.5 7 3013.5 8 4532.5 9 4790.0 10 3872.5 11 2740.0 12 2473.0 13 2626.0 14 2752.0 15 2840.5 16 3010.0 17 3205.0 18 3485.0 19 3782.0 20 4136.5 21 4729.0 22 5509.0 23 6515.5 24 7846.0 25 9624.0 26 12169.5 27 14979.5 28 17192.5 29 19543.0 30 22641.0 31 27563.5 32 35155.5 33 37957.5 34 37022.0 35 38721.5 36 42659.0 37 47107.5 38 50365.0 39 53126.0 40 55697.0 41 58945.0 42 60933.5 43 62007.5 44 63893.0 45 68010.0 46 70744.5 47 73173.0 48 77000.0 49 76383.0 50 73973.0 51 72443.0 52 72367.0 53 74769.0 54 77095.0 55 74667.5 56 69856.5 57 64360.0 58 59340.0 59 53662.0 60 44473.5 61 37064.5 62 31685.5 63 23835.0 64 15995.5 65 11743.0 66 9117.5 67 7042.0 68 5360.0 69 4097.5 70 3124.0 71 2157.0 72 1506.0 73 1211.5 74 921.0 75 658.0 76 483.0 77 342.0 78 229.5 79 142.0 80 91.0 81 62.0 82 36.5 83 29.5 84 20.5 85 8.5 86 6.5 87 3.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024076703533624278 2 0.010447484768042093 3 0.001472145580951386 4 0.0 5 4.748856712746406E-4 6 0.0 7 0.0 8 4.748856712746407E-5 9 3.7990853701971253E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001495889864515118 26-27 0.009378992007674152 28-29 1.424657013823922E-4 30-31 0.0 32-33 3.561642534559805E-4 34-35 0.01063743903655195 36-37 0.0013771684466964578 38-39 0.013154333094307545 40-41 0.013035611676488887 42-43 0.005247486667584778 44-45 8.547942082943531E-4 46-47 4.748856712746407E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.12328506911961E-5 66-67 5.698628055295688E-4 68-69 0.0010447484768042095 70-71 4.748856712746407E-5 72-73 1.662099849461242E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.748856712746407E-5 82-83 0.0013771684466964578 84-85 0.006292235144388988 86-87 0.0044639253099816225 88-89 0.0 90-91 0.0011397256110591376 92-93 7.12328506911961E-4 94-95 2.3744283563732033E-5 96-97 4.748856712746407E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2105770.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.121414589058574 #Duplication Level Percentage of deduplicated Percentage of total 1 70.98098269899579 31.317813656011868 2 14.551449565729824 12.840610783226836 3 5.522851025955344 7.310279994893497 4 2.7091046728394037 4.781181217420129 5 1.533674379963148 3.38339415814857 6 0.9927817787959934 2.6281761875232634 7 0.6706101867705814 2.0711789054705574 8 0.4515993832358903 1.5940162892731087 9 0.3652092036089171 1.4502192015752045 >10 2.0017687544647593 16.125520097968423 >50 0.14203883800546796 4.263481096659759 >100 0.06809571142589575 5.7155166951600656 >500 0.0046467407581243575 1.4783905081901985 >1k 0.00486286823524642 3.761702116753439 >5k 2.161274771220631E-4 0.765967153032397 >10k+ 1.0806373856103154E-4 0.5125519386927027 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10750 0.5105020966202386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9271 0.44026650583871935 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6794 0.32263732506399084 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3257 0.15467026313415044 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3244 0.1540529117614934 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3192 0.15158350627086528 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2916 0.1384766617436852 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2915 0.13842917317655773 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2677 0.12712689420022127 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2669 0.12674698566320158 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2261 0.10737165027519624 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2208 0.10485475621744066 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2207 0.10480726765031319 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2142 0.10172051078702801 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2114 0.10039083090745904 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2107 0.10005841093756677 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.497713425492812E-5 2 0.0 0.0 0.0 0.0 9.497713425492812E-5 3 0.0 0.0 0.0 0.0 9.497713425492812E-5 4 0.0 0.0 0.0 0.0 9.497713425492812E-5 5 0.0 0.0 0.0 0.0 9.497713425492812E-5 6 0.0 0.0 0.0 0.0 1.424657013823922E-4 7 0.0 0.0 0.0 0.0 1.424657013823922E-4 8 0.0 0.0 0.0 0.0 1.424657013823922E-4 9 0.0 0.0 0.0 0.0 1.424657013823922E-4 10-11 0.0 0.0 0.0 4.748856712746406E-5 1.424657013823922E-4 12-13 0.0 0.0 0.0 4.748856712746406E-5 3.3241996989224847E-4 14-15 0.0 0.0 0.0 4.748856712746406E-5 3.799085370197125E-4 16-17 0.0 0.0 0.0 9.497713425492812E-5 3.799085370197125E-4 18-19 0.0 0.0 0.0 9.497713425492812E-5 3.799085370197125E-4 20-21 0.0 0.0 0.0 1.8995426850985627E-4 3.799085370197125E-4 22-23 0.0 0.0 0.0 7.598170740394251E-4 3.799085370197125E-4 24-25 0.0 0.0 0.0 0.0018045655508436343 3.799085370197125E-4 26-27 0.0 0.0 0.0 0.0033479439824862164 4.511413877109086E-4 28-29 0.0 0.0 0.0 0.007289495054065734 4.748856712746406E-4 30-31 0.0 0.0 0.0 0.019517801089387728 4.748856712746406E-4 32-33 0.0 0.0 0.0 0.04050774775972685 4.748856712746406E-4 34-35 0.0 0.0 0.0 0.07144654924326968 5.223742384021047E-4 36-37 0.0 0.0 0.0 0.11950497917626332 5.223742384021047E-4 38-39 0.0 0.0 0.0 0.19256613970186676 5.698628055295688E-4 40-41 0.0 0.0 0.0 0.2769295792038067 5.698628055295688E-4 42-43 0.0 0.0 0.0 0.36100808730298184 5.698628055295688E-4 44-45 0.0 0.0 0.0 0.4620400138666616 6.173513726570329E-4 46-47 0.0 0.0 0.0 0.5887395109627357 6.648399397844969E-4 48-49 0.0 0.0 0.0 0.7185020206385313 6.648399397844969E-4 50-51 0.0 0.0 0.0 0.8659302772857435 7.123285069119609E-4 52-53 0.0 0.0 0.0 1.0059028288939438 8.07305641166889E-4 54-55 0.0 0.0 0.0 1.159623320685545 8.07305641166889E-4 56-57 0.0 2.374428356373203E-5 0.0 1.3392725701287413 8.07305641166889E-4 58-59 0.0 4.748856712746406E-5 0.0 1.5262350589095675 8.07305641166889E-4 60-61 0.0 4.748856712746406E-5 0.0 1.7368468541198707 8.547942082943531E-4 62-63 0.0 4.748856712746406E-5 2.374428356373203E-5 1.9476011150315562 9.022827754218172E-4 64-65 0.0 4.748856712746406E-5 4.748856712746406E-5 2.160801037150306 9.022827754218172E-4 66-67 0.0 4.748856712746406E-5 4.748856712746406E-5 2.407456654810354 9.022827754218172E-4 68-69 0.0 4.748856712746406E-5 4.748856712746406E-5 2.6858821238786765 9.022827754218172E-4 70-71 0.0 9.497713425492812E-5 4.748856712746406E-5 2.985558726736538 9.022827754218172E-4 72-73 0.0 9.497713425492812E-5 4.748856712746406E-5 3.292002450410064 9.260270589855491E-4 74-75 0.0 9.497713425492812E-5 4.748856712746406E-5 3.6024352137222966 0.0010210041932404773 76-77 0.0 9.497713425492812E-5 4.748856712746406E-5 3.9318634038855147 0.0010922370439316734 78-79 0.0 9.497713425492812E-5 4.748856712746406E-5 4.307545458430883 0.0010922370439316734 80-81 0.0 9.497713425492812E-5 4.748856712746406E-5 4.708538919255189 0.0011159813274954056 82-83 0.0 9.497713425492812E-5 4.748856712746406E-5 5.117510459356909 0.0012347027453140657 84-85 0.0 9.497713425492812E-5 4.748856712746406E-5 5.537570579882893 0.0012347027453140657 86-87 0.0 9.497713425492812E-5 4.748856712746406E-5 5.962403301405187 0.00128219131244153 88-89 0.0 9.497713425492812E-5 4.748856712746406E-5 6.413236013429767 0.0013534241631327259 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7845 0.0 36.217457 1 GTATCAA 12075 0.0 34.03601 1 GTAAGGT 1250 0.0 27.358866 4 TAAGGTG 1420 0.0 27.093948 5 AGGTAAG 1250 0.0 26.980803 2 TATCAAC 15200 0.0 26.813295 2 ATCAACG 15450 0.0 26.285318 3 TCAACGC 15595 0.0 26.040918 4 CAACGCA 15825 0.0 25.662441 5 AACGCAG 16245 0.0 25.05744 6 GGTAAGG 1450 0.0 24.567945 3 GTACATG 10515 0.0 24.129128 1 TACATGG 10340 0.0 24.072283 2 AAGGTAA 1470 0.0 22.301878 1 ACATGGG 10490 0.0 22.232157 3 ACGCAGA 18485 0.0 21.866823 7 CGCAGAG 18585 0.0 21.749165 8 GAGTACT 10065 0.0 21.330462 12-13 AGTACTT 10550 0.0 21.115236 12-13 AGAGTAC 15535 0.0 21.035498 10-11 >>END_MODULE