FastQCFastQC Report
Thu 26 May 2016
SRR938511_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938511_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988157
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51010.5162135166780177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42620.43130798041201956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26690.2700987798497607No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10090.10210928020547341No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27200.047.544851
GTATCAA44400.037.15781
TAGGGCG600.00393247331.696575
TATCAAC52350.031.4241032
TCAACGC52300.031.0905154
ATCAACG52450.031.00163
CAACGCA52500.030.8815145
AACGCAG54250.029.9532416
ACGCAGA61250.026.6089347
CGCAGAG62100.026.0916928
GTAGCCC7700.024.081163
GTGTAGC8550.023.9114461
GAGTACT43200.023.53561212-13
GCAGAGT65550.023.4849579
TAGCCCT12100.022.3971614
GTACTTT46100.022.00241914-15
CAGAGTA65400.021.14188410-11
AGTACTT43100.020.44853212-13
TAGGACC5150.020.3104234
GTACATG34150.020.0481971