##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938511_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 988157 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.305218705124794 31.0 31.0 34.0 28.0 34.0 2 31.364787174507693 31.0 31.0 34.0 28.0 34.0 3 31.391702937893474 31.0 31.0 34.0 28.0 34.0 4 35.05453283233332 37.0 35.0 37.0 32.0 37.0 5 35.056123672655254 37.0 35.0 37.0 32.0 37.0 6 35.086150277739264 37.0 35.0 37.0 32.0 37.0 7 35.01487921453777 37.0 35.0 37.0 32.0 37.0 8 35.00509028423621 37.0 35.0 37.0 32.0 37.0 9 36.53906818450914 39.0 37.0 39.0 32.0 39.0 10-11 36.400190455565266 39.0 37.0 39.0 32.0 39.0 12-13 36.30643713498968 39.0 35.0 39.0 32.0 39.0 14-15 37.316451231939865 39.5 37.0 41.0 32.0 41.0 16-17 37.177591212732395 39.0 36.0 41.0 31.5 41.0 18-19 37.349006787382976 39.5 37.0 41.0 32.0 41.0 20-21 37.37080646091664 40.0 37.0 41.0 32.0 41.0 22-23 37.28448667570032 40.0 37.0 41.0 31.5 41.0 24-25 37.19296731187453 39.5 36.5 41.0 31.0 41.0 26-27 37.02512151409138 39.0 36.0 41.0 31.0 41.0 28-29 36.843977728235494 39.0 36.0 41.0 30.5 41.0 30-31 36.611268249883366 39.0 36.0 40.5 30.0 41.0 32-33 36.35544048162387 39.0 35.0 40.0 30.0 41.0 34-35 36.13040083711394 38.5 35.0 40.0 30.0 41.0 36-37 35.89449247437401 38.0 35.0 40.0 29.0 41.0 38-39 35.77359366983182 38.0 35.0 40.0 28.5 41.0 40-41 35.6791127320861 38.0 35.0 40.0 27.5 41.0 42-43 35.403333174789026 38.0 35.0 40.0 26.5 41.0 44-45 35.03655643789398 38.0 34.0 40.0 25.5 41.0 46-47 34.62167347901194 38.0 34.0 40.0 24.0 41.0 48-49 34.26831313242734 38.0 33.0 40.0 23.0 41.0 50-51 33.586239332413776 37.5 33.0 39.5 21.5 40.5 52-53 33.634840415035264 38.0 33.0 39.5 21.0 40.5 54-55 33.80712680272467 38.0 33.0 40.0 19.5 41.0 56-57 33.56001829668767 38.0 33.0 40.0 17.5 41.0 58-59 33.27655018382706 38.0 33.0 40.0 14.5 41.0 60-61 32.91069587120265 37.5 32.0 40.0 11.0 41.0 62-63 32.43707578856396 37.0 31.0 40.0 8.0 41.0 64-65 32.02390156624909 36.5 31.0 39.0 7.5 40.5 66-67 31.587019572800678 36.0 31.0 39.0 2.0 40.0 68-69 31.10096826718831 35.5 29.5 39.0 2.0 40.0 70-71 30.554762046921695 35.0 29.0 38.0 2.0 40.0 72-73 29.965255521136825 35.0 28.5 38.0 2.0 39.5 74-75 29.3450433483748 34.0 28.0 37.0 2.0 39.0 76-77 28.637931523027213 34.0 26.5 36.5 2.0 39.0 78-79 27.89425566989861 33.5 26.0 36.0 2.0 38.5 80-81 27.224762360637023 33.0 25.5 35.0 2.0 37.0 82-83 26.53004431482042 32.0 23.5 35.0 2.0 37.0 84-85 25.833705575126217 32.0 21.0 35.0 2.0 36.0 86-87 25.100108585983808 31.0 19.0 34.5 2.0 36.0 88-89 24.33001992598342 31.0 12.5 34.0 2.0 35.0 90-91 23.45023311073038 30.5 4.5 34.0 2.0 35.0 92-93 22.547813252347552 30.0 2.0 34.0 2.0 35.0 94-95 21.59023161299267 29.0 2.0 34.0 2.0 35.0 96-97 20.391310287737678 28.5 2.0 34.0 2.0 35.0 98-99 18.914423517720365 26.0 2.0 33.5 2.0 35.0 100-101 15.246778092954866 11.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6316857753664067 1101 2 0.629162607125437 1101 3 0.5216397567183044 1101 4 0.42288017984342474 1101 5 0.6222561923085976 1101 6 0.81174995254392 1101 7 0.7095207496510696 1101 8 0.8560282231698366 1101 9 0.941113978636011 1101 10-11 0.9376249572923001 1101 12-13 0.8747181568429312 1101 14-15 1.1276566713733729 1101 16-17 1.0945440883297195 1101 18-19 1.249367647644604 1101 20-21 1.1720441323484678 1101 22-23 1.1095297533907384 1101 24-25 1.1133929586970694 1101 26-27 1.2734924613600427 1101 28-29 1.1993867446436965 1101 30-31 0.9727760708265905 1101 32-33 1.2431410884249985 1101 34-35 1.3283032064788145 1101 36-37 1.3591721306534836 1101 38-39 1.437802018410352 1101 40-41 1.6023090099048503 1101 42-43 1.6626430912944485 1101 44-45 1.6159499163510347 1101 46-47 1.6086580189595239 1101 48-49 1.4455918148526834 1101 50-51 1.4536742524261541 1101 52-53 1.4525204156253508 1101 54-55 1.361153989135822 1101 56-57 1.1237521687007757 1101 58-59 1.2835999667392528 1101 60-61 1.5025282704538796 1101 62-63 1.2045391811746171 1101 64-65 1.434025648032943 1101 66-67 1.3796441562272754 1101 68-69 1.24285322281332 1101 70-71 1.1880777446067938 1101 72-73 1.1638735498091783 1101 74-75 1.1333482475048164 1101 76-77 1.0093860859157822 1101 78-79 1.0142209264620092 1101 80-81 1.137876767070889 1101 82-83 1.2213655008372442 1101 84-85 0.9180044005248824 1101 86-87 1.2341911878355596 1101 88-89 1.2483100231504096 1101 90-91 1.361391960952787 1101 92-93 1.4089740644215958 1101 94-95 1.3713419061276575 1101 96-97 1.5249758615782696 1101 98-99 1.9282968487279852 1101 100-101 1.8430749075794868 1102 1 0.6169471417656283 1102 2 0.5163349873831535 1102 3 0.5950411668812023 1102 4 0.5366997762450794 1102 5 0.582163802325617 1102 6 0.6085503415543769 1102 7 0.6075124831789083 1102 8 0.6041731295641952 1102 9 0.7637179173668684 1102 10-11 0.7881100047029506 1102 12-13 0.7692209528555694 1102 14-15 0.8920561364839941 1102 16-17 0.9582810200097569 1102 18-19 0.8695888973406909 1102 20-21 0.9407336533793398 1102 22-23 1.0510505411369095 1102 24-25 1.0008619594264658 1102 26-27 0.7349998768718322 1102 28-29 0.8714813228104887 1102 30-31 0.903719421179126 1102 32-33 0.8665680273989835 1102 34-35 0.7525701649033181 1102 36-37 1.0785570078720568 1102 38-39 0.9713164847366329 1102 40-41 1.0872093259758415 1102 42-43 1.1132436261838237 1102 44-45 1.4023309034650708 1102 46-47 1.0202663340579932 1102 48-49 1.2229076427155618 1102 50-51 1.1625886701261265 1102 52-53 1.2688989714240435 1102 54-55 1.1357321559240532 1102 56-57 1.105796904903066 1102 58-59 1.03665491446597 1102 60-61 1.2358458002374846 1102 62-63 1.2621163754367117 1102 64-65 1.284039020267322 1102 66-67 1.0723915425632846 1102 68-69 1.0969864588940332 1102 70-71 1.6005418827054996 1102 72-73 1.5858514248395714 1102 74-75 1.4628741410132058 1102 76-77 1.5232701121739893 1102 78-79 1.5039838732162032 1102 80-81 1.6684529888068447 1102 82-83 1.4866256515787946 1102 84-85 1.4103056404957037 1102 86-87 1.644298165710591 1102 88-89 1.8900301787545928 1102 90-91 1.7262643168973568 1102 92-93 1.8071214021312976 1102 94-95 1.7658580743746803 1102 96-97 1.8792695644715351 1102 98-99 1.809237767765179 1102 100-101 2.0864860430037506 1103 1 0.3624273058094296 1103 2 0.33669162868430647 1103 3 0.20815953876047644 1103 4 0.3035672208289739 1103 5 0.23400696226832451 1103 6 0.43164571184266265 1103 7 0.3323542014397418 1103 8 0.44866977730392676 1103 9 0.4108581150965662 1103 10-11 0.6071381058352969 1103 12-13 0.5656160483378301 1103 14-15 0.6205078914633972 1103 16-17 0.6180219371806999 1103 18-19 0.5192065483790245 1103 20-21 0.3079811141063402 1103 22-23 0.5219497960346686 1103 24-25 0.5615024114333877 1103 26-27 0.6986428996448097 1103 28-29 0.6596509000571089 1103 30-31 0.610951858554607 1103 32-33 0.7649096266860766 1103 34-35 0.6393479825982169 1103 36-37 0.5624675582772696 1103 38-39 0.7665428381213175 1103 40-41 0.7236582162540586 1103 42-43 0.867201252867531 1103 44-45 0.8082728256142389 1103 46-47 0.7036153750362857 1103 48-49 0.6580296257979583 1103 50-51 0.935018720561672 1103 52-53 0.7413157247938003 1103 54-55 0.5558424515854838 1103 56-57 0.8651760019156569 1103 58-59 0.5203578440195216 1103 60-61 0.8030601349409636 1103 62-63 0.6442110598763477 1103 64-65 0.5999306468483852 1103 66-67 0.8183056108588751 1103 68-69 0.9565111237846473 1103 70-71 0.9018079033369517 1103 72-73 0.9512734550449018 1103 74-75 0.9057434145268495 1103 76-77 0.8721046360223923 1103 78-79 0.7088939897171649 1103 80-81 0.766796454348782 1103 82-83 0.9223261736635884 1103 84-85 1.1360676556776887 1103 86-87 1.086676380917769 1103 88-89 1.0688418876375003 1103 90-91 0.860915770476602 1103 92-93 0.9540131546845636 1103 94-95 0.9660978881224445 1103 96-97 0.9911142174181151 1103 98-99 1.0189791514998383 1103 100-101 1.2274151113226726 1104 1 0.406523594552052 1104 2 0.33559462920963057 1104 3 0.3727094599619214 1104 4 0.33193470020229654 1104 5 0.3091437009352376 1104 6 0.41114263715399346 1104 7 0.24756077725631087 1104 8 0.45282601475023654 1104 9 0.5180705003751456 1104 10-11 0.5242605680583452 1104 12-13 0.6295973838960691 1104 14-15 0.8203240281711643 1104 16-17 0.8258415348445496 1104 18-19 0.6907479098944265 1104 20-21 0.6214989287597916 1104 22-23 0.695399318608132 1104 24-25 0.5995420270200569 1104 26-27 0.6730024682613518 1104 28-29 0.7312880558928399 1104 30-31 0.6864130618548856 1104 32-33 0.541322592910852 1104 34-35 0.7344315708680469 1104 36-37 0.8340444563953682 1104 38-39 0.8996274152357415 1104 40-41 0.6651696034176169 1104 42-43 0.519861862861049 1104 44-45 0.4455599922654585 1104 46-47 0.6334923874895466 1104 48-49 0.7982833262398472 1104 50-51 0.8060517541514827 1104 52-53 0.6965473307392216 1104 54-55 0.9885314908611562 1104 56-57 0.8568094495841478 1104 58-59 0.7335620095273825 1104 60-61 0.7793433153124028 1104 62-63 0.9567786566749632 1104 64-65 1.0354162622496474 1104 66-67 1.0326531279318978 1104 68-69 1.2395060868574852 1104 70-71 1.148702313274839 1104 72-73 1.230598877621464 1104 74-75 1.322665017691154 1104 76-77 1.331963725667336 1104 78-79 1.2387447359858186 1104 80-81 1.091106580735481 1104 82-83 1.0173247831008716 1104 84-85 1.081016822885129 1104 86-87 0.9912374266209305 1104 88-89 0.6993693744271532 1104 90-91 1.0027377166463722 1104 92-93 1.2071728221855693 1104 94-95 1.1384813126148892 1104 96-97 1.1770324523305113 1104 98-99 1.7920856686037574 1104 100-101 2.0216118295946703 1105 1 0.4284635531441836 1105 2 0.4792737182365485 1105 3 0.659417534496086 1105 4 0.4561024020656461 1105 5 0.5593673034197053 1105 6 0.45486106365503076 1105 7 0.6004096385399578 1105 8 0.5269171120587259 1105 9 0.8131139786360109 1105 10-11 0.7032916239589682 1105 12-13 0.8603292679540431 1105 14-15 0.9663788935955964 1105 16-17 0.98015519944083 1105 18-19 0.82331209208904 1105 20-21 0.9459885767929137 1105 22-23 0.883029753390737 1105 24-25 0.8392262920304034 1105 26-27 1.1785480169155989 1105 28-29 0.9852756335325878 1105 30-31 1.0882205152710327 1105 32-33 1.174085532869448 1105 34-35 1.246803206478809 1105 36-37 1.1067276862090338 1105 38-39 1.0847464628547954 1105 40-41 1.2300867876826302 1105 42-43 1.2889208690722285 1105 44-45 1.370394360795487 1105 46-47 1.3726580189595197 1105 48-49 1.3074251481860202 1105 50-51 1.0982298079817099 1105 52-53 1.1310204156253505 1105 54-55 0.9705428780247161 1105 56-57 1.084529946478554 1105 58-59 1.2330444111836982 1105 60-61 1.1944727148983247 1105 62-63 1.299650292285726 1105 64-65 1.4499700924773933 1105 66-67 1.6203663784494928 1105 68-69 1.2804087783688693 1105 70-71 1.4145221890512332 1105 72-73 1.455929105364735 1105 74-75 1.4236815808381529 1105 76-77 1.470886085915783 1105 78-79 1.6163875931286746 1105 80-81 1.7557101004042188 1105 82-83 1.8904210563927997 1105 84-85 1.8371710671915515 1105 86-87 1.7167467433911163 1105 88-89 1.7707544675948554 1105 90-91 1.9575586276194556 1105 92-93 2.1668073977549263 1105 94-95 2.2573974616832153 1105 96-97 2.5558647504671583 1105 98-99 2.7897968487279847 1105 100-101 2.3796304631350385 1106 1 0.26846355314418346 1106 2 0.1012737182365484 1106 3 0.05341753449608433 1106 4 0.13210240206564805 1106 5 0.1393673034197107 1106 6 -0.04913893634496702 1106 7 0.2734096385399596 1106 8 0.3049171120587246 1106 9 0.20211397863601377 1106 10-11 0.10879162395897168 1106 12-13 0.18782926795403654 1106 14-15 0.36587889359559966 1106 16-17 0.10515519944082996 1106 18-19 0.07281209208904471 1106 20-21 0.2754885767929096 1106 22-23 0.16402975339074288 1106 24-25 0.045226292030399406 1106 26-27 0.09204801691559794 1106 28-29 0.3437756335325872 1106 30-31 -0.021779484728966736 1106 32-33 0.2485855328694484 1106 34-35 0.04880320647880865 1106 36-37 0.02672768620903554 1106 38-39 0.0907464628547956 1106 40-41 0.22558678768262297 1106 42-43 -0.24107913092777267 1106 44-45 0.02139436079548318 1106 46-47 0.02465801895952069 1106 48-49 0.39592514818601643 1106 50-51 -0.03127019201829029 1106 52-53 -0.09547958437465098 1106 54-55 -0.037957121975289 1106 56-57 -0.6464700535214405 1106 58-59 -0.7029555888163017 1106 60-61 0.2714727148983158 1106 62-63 0.1656502922857257 1106 64-65 0.13947009247738862 1106 66-67 -0.2696336215505042 1106 68-69 -0.18759122163112707 1106 70-71 -0.30847781094876225 1106 72-73 0.15542910536473542 1106 74-75 0.5136815808381492 1106 76-77 0.30838608591578165 1106 78-79 0.35838759312867907 1106 80-81 0.5797101004042204 1106 82-83 0.4949210563927977 1106 84-85 0.37217106719154813 1106 86-87 0.38424674339111675 1106 88-89 0.6982544675948539 1106 90-91 0.47205862761945383 1106 92-93 0.5728073977549286 1106 94-95 0.4548974616832133 1106 96-97 0.3563647504671579 1106 98-99 0.8132968487279832 1106 100-101 0.6031304631350398 1107 1 0.23829720515183084 1107 2 0.1575586130740234 1107 3 0.17781141594923966 1107 4 0.25971043265838034 1107 5 0.25745908162238607 1107 6 0.2871822873644021 1107 7 0.3665549540275279 1107 8 0.06809110823463271 1107 9 0.4378061392478685 1107 10-11 0.5047600752017942 1107 12-13 0.3756763042829121 1107 14-15 0.4483014557370879 1107 16-17 0.5613913371081338 1107 18-19 0.6773111360660948 1107 20-21 0.5978843702919576 1107 22-23 0.4063270765265017 1107 24-25 0.6376631945160653 1107 26-27 0.4729868314471517 1107 28-29 0.6634668381215008 1107 30-31 0.6062644923264813 1107 32-33 0.7377862976877978 1107 34-35 0.7039437418516599 1107 36-37 0.5149762521746197 1107 38-39 0.6867302104647379 1107 40-41 0.5460428106271777 1107 42-43 0.6482984981353255 1107 44-45 0.6609392938738807 1107 46-47 0.6580748449633447 1107 48-49 0.25664981357798666 1107 50-51 0.3955873605629705 1107 52-53 0.25187988025250263 1107 54-55 0.4018402011604678 1107 56-57 0.4190251663638378 1107 58-59 0.4371553098452665 1107 60-61 0.2812652579193511 1107 62-63 0.4960805026107735 1107 64-65 0.3960704748865638 1107 66-67 0.5601264166904123 1107 68-69 0.6536669045639023 1107 70-71 0.7625699901984646 1107 72-73 0.7705667726687473 1107 74-75 0.7752666668802171 1107 76-77 0.5561451681337566 1107 78-79 0.8425415128227485 1107 80-81 0.7765829493525978 1107 82-83 0.7488426625113469 1107 84-85 0.9011815834439396 1107 86-87 0.9659800129895864 1107 88-89 1.0632994006732481 1107 90-91 1.075611208881405 1107 92-93 1.202020590871566 1107 94-95 1.1344404827157177 1107 96-97 1.498778708402149 1107 98-99 1.586537766510009 1107 100-101 1.6618235797698393 1108 1 0.36672917814418327 1108 2 0.25113309323654676 1108 3 0.1687534719960837 1108 4 0.4006883395656473 1108 5 0.17067199091970764 1108 6 0.29446262615503116 1108 7 0.4051674510399579 1108 8 0.5708546120587243 1108 9 0.357871791136013 1108 10-11 0.4179244364589678 1108 12-13 0.3967862992040381 1108 14-15 0.5493906123455972 1108 16-17 0.6635653556908281 1108 18-19 0.6401597483390447 1108 20-21 0.5166448267929127 1108 22-23 0.4433188158907413 1108 24-25 0.7174098857804054 1108 26-27 0.6109230169155992 1108 28-29 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-0.9609938056139953 2316 52-53 -1.1067014090615928 2316 54-55 -1.2680787677713496 2316 56-57 -1.3190425043264469 2316 58-59 -1.2034493276356244 2316 60-61 -1.1441632421678705 2316 62-63 -1.0460760046731288 2316 64-65 -1.09422221521492 2316 66-67 -1.514663138545135 2316 68-69 -1.4866538334379271 2316 70-71 -1.5857336249022538 2316 72-73 -1.4615458499125502 2316 74-75 -1.498667256371153 2316 76-77 -1.4928232164097999 2316 78-79 -1.7462412083024539 2316 80-81 -1.9604893629231483 2316 82-83 -2.377560160065162 2316 84-85 -2.4092376984614 2316 86-87 -2.2064786591133547 2316 88-89 -2.4280237077182036 2316 90-91 -2.3937481702338523 2316 92-93 -2.6705906344454284 2316 94-95 -2.897369085722868 2316 96-97 -2.8172980402305186 2316 98-99 -2.6717389294473293 2316 100-101 -3.009330180872114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4082.0 3 867.0 4 783.0 5 814.0 6 954.0 7 1174.0 8 1473.0 9 1963.0 10 2566.0 11 3663.0 12 4893.0 13 6457.0 14 7810.0 15 9553.0 16 11615.0 17 14064.0 18 12490.0 19 10161.0 20 10265.0 21 10915.0 22 11987.0 23 13427.0 24 15224.0 25 17311.0 26 19506.0 27 22637.0 28 26182.0 29 30484.0 30 36225.0 31 42165.0 32 50836.0 33 60881.0 34 75146.0 35 94742.0 36 121903.0 37 144366.0 38 83814.0 39 4759.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.50587649493113 20.616766303440276 15.12900153731091 22.74835566431769 2 15.185507105432707 21.676432331238377 40.91023287665682 22.227827686672097 3 18.227233307937947 25.515776207226338 31.36731857159067 24.889671913245042 4 12.117349910995703 18.388176257888556 38.933528936781116 30.560944894334625 5 12.614032438371048 38.081285453813884 35.727714709361074 13.576967398453993 6 29.555710649229095 37.87252448043338 18.812682520661618 13.759082349675907 7 26.119638670807404 33.90457411013981 22.428505488365314 17.547281730687473 8 24.012872191167627 36.04750036948331 21.551809658394966 18.387817780954098 9 25.196825686030387 16.867996720415416 22.181935967123177 35.75324162643102 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28.76095822268577 31.203246393247987 19.751457510069034 36-37 20.198109725814557 28.553022398468258 30.936140849676676 20.312727026040502 38-39 19.836852950510746 28.843398167987 31.101855043502336 20.21789383799992 40-41 20.621260876013572 28.335264428418633 31.102323742649563 19.941150952918232 42-43 20.117906215612376 28.45268212091486 30.939726812300634 20.489684851172125 44-45 20.4198453968209 28.591720491702073 30.724345648825295 20.264088462651735 46-47 20.30087325813948 28.57450831153899 30.30047853088589 20.824139899435643 48-49 20.305060438745773 28.495855289092386 30.42624155357964 20.7728427185822 50-51 20.485236444601878 28.80990815474534 29.835437593300103 20.86941780735268 52-53 20.328404100598046 28.96783668049524 29.866828736573314 20.8369304823334 54-55 20.60392750238479 28.705634683069636 29.794768896772123 20.895668917773456 56-57 20.667056343766053 28.80867613531411 29.475226404925113 21.049041115994726 58-59 20.480806361086074 28.799138008258563 29.591034719055575 21.129020911599792 60-61 20.618280154936986 28.528761683413716 29.69944391328752 21.15351424836178 62-63 20.491339118219052 28.310853517766837 29.843391096161113 21.354416267852994 64-65 20.40124183056148 28.368389491502267 29.64341650157 21.586952176366253 66-67 20.283448054596594 28.434173677972808 29.547531645761794 21.734846621668808 68-69 20.52607661095654 28.1287226817782 29.67027350356416 21.6749272037011 70-71 20.28499453607783 27.838215263855055 29.807859240861532 22.06893095920558 72-73 20.210899689531846 27.72428970413914 29.86041586738242 22.204394738946593 74-75 20.336963602322818 27.771836151486333 29.520327701076525 22.37087254511432 76-77 20.180886922897834 27.659098500875583 29.706197933010092 22.453816643216488 78-79 20.096356449030566 27.601350872683184 29.583231285838913 22.719061392447337 80-81 20.045319139138766 27.633441233635953 29.40678785167748 22.914451775547803 82-83 20.271622167987974 27.617079436757695 29.56217510263379 22.54912329262054 84-85 20.253203629599493 27.305388736744735 29.530724685203293 22.910682948452482 86-87 20.010647703228674 27.292482387514656 29.523186335312573 23.1736835739441 88-89 20.531553187967617 26.92925430597463 29.52293744232674 23.016255063731005 90-91 20.435931167377746 27.009318026171865 29.319666152569834 23.235084653880552 92-93 20.234576943069563 26.720898180770924 29.63779914937641 23.406725726783105 94-95 20.255968670617264 26.795460965334584 29.313282690374294 23.63528767367386 96-97 20.350016806314315 26.538132109779657 29.215043878654363 23.89680720525167 98-99 20.602472325022067 26.221940366192392 29.10523982281536 24.070347485970185 100-101 20.885503883073827 25.857316974984972 29.32673976240257 23.93043937953863 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 362.0 1 210.0 2 87.0 3 103.5 4 79.5 5 89.5 6 164.5 7 287.0 8 432.5 9 514.0 10 498.0 11 492.5 12 525.5 13 622.5 14 763.5 15 888.5 16 1049.5 17 1232.0 18 1459.0 19 1779.5 20 2086.5 21 2513.0 22 3083.0 23 3778.5 24 4746.5 25 5965.5 26 7535.0 27 9568.0 28 11710.0 29 13861.0 30 16374.0 31 19106.0 32 22088.0 33 24711.0 34 27654.5 35 31007.0 36 33835.0 37 36309.5 38 37154.5 39 37207.5 40 37505.5 41 37721.0 42 37972.5 43 38440.0 44 38589.5 45 38617.5 46 39118.0 47 39030.0 48 38623.0 49 37368.5 50 35504.0 51 33162.0 52 30114.0 53 27060.5 54 23834.5 55 20565.5 56 17130.5 57 13888.5 58 10912.5 59 8434.0 60 6430.5 61 4582.5 62 3247.0 63 2368.0 64 1683.5 65 1175.0 66 826.0 67 589.5 68 453.5 69 348.0 70 239.0 71 176.0 72 156.5 73 136.5 74 108.5 75 78.0 76 48.5 77 30.0 78 28.5 79 28.5 80 21.5 81 18.0 82 14.0 83 6.0 84 3.5 85 4.5 86 4.0 87 2.5 88 1.0 89 0.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006881497575790083 2 0.10372845610565933 3 0.11141954163154237 4 0.11394950397558283 5 0.15038096172976562 6 0.09512658413592172 7 0.025198424946643094 8 0.029448761684631085 9 0.02297205808388748 10-11 0.05125703709025995 12-13 0.04984025817759729 14-15 0.027627188796921946 16-17 0.029195765450227038 18-19 0.03420509089142717 20-21 0.056620557259625746 22-23 0.02929696394398866 24-25 0.047259696586676 26-27 0.05424239265622771 28-29 0.05135823558402156 30-31 0.0665886088951452 32-33 0.04751269282108005 34-35 0.0606684970100905 36-37 0.05287621299044585 38-39 0.034053293150784744 40-41 0.03800003440748788 42-43 0.03420509089142717 44-45 0.00799468100716789 46-47 0.013459399670295307 48-49 0.009158463685426506 50-51 0.008298276488452746 52-53 0.010727040338731599 54-55 0.012599212473321547 56-57 0.016141159754978207 58-59 0.021504679924344006 60-61 0.027475391056279518 62-63 0.012396815485798308 64-65 0.010069250129281076 66-67 0.011131834313778075 68-69 0.012751010213963973 70-71 0.03172572779426751 72-73 0.02975235716591594 74-75 0.03091613984417456 76-77 0.024996027959119857 78-79 0.0055659171568890374 80-81 0.005414119416246609 82-83 0.005717714897531465 84-85 0.005970711131935512 86-87 0.01523037331112364 88-89 0.03769643892620302 90-91 0.03445808712583122 92-93 0.029701757919035135 94-95 0.046348910142821435 96-97 0.04381894779878096 98-99 0.026412806871782518 100-101 0.07392549969286258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 988157.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.92022608978044 #Duplication Level Percentage of deduplicated Percentage of total 1 78.29189686465328 54.7418712977433 2 13.747664852896513 19.224796694421048 3 4.3299986856781345 9.08263461213202 4 1.7500912324875526 4.894670986130888 5 0.7920510230348005 2.7690193302617576 6 0.3955097417957667 1.6592478340242416 7 0.22718961370998522 1.1119604409096433 8 0.1354507511969127 0.7576597718174988 9 0.08555957650384094 0.5384110439964983 >10 0.2273462252898848 2.518488469187092 >50 0.009420524039727693 0.4625401350942722 >100 0.0069519197043979505 0.8031452914079289 >500 2.8966300307285E-4 0.11493749262216364 >1k 4.34494504609275E-4 0.804056115788528 >5k 1.44831501536425E-4 0.5165604844631337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5101 0.5162135166780177 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4262 0.43130798041201956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2669 0.2700987798497607 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1009 0.10210928020547341 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.0599246880809424E-5 0.0 12-13 0.0 0.0 0.0 1.0119849376161885E-4 0.0 14-15 0.0 0.0 0.0 1.0119849376161885E-4 0.0 16-17 0.0 0.0 0.0 1.0119849376161885E-4 0.0 18-19 0.0 0.0 0.0 1.0119849376161885E-4 0.0 20-21 0.0 0.0 0.0 1.5179774064242826E-4 0.0 22-23 0.0 0.0 0.0 8.601871969737603E-4 0.0 24-25 0.0 0.0 0.0 0.0020745691221131864 0.0 26-27 0.0 0.0 0.0 0.0037443442691798973 0.0 28-29 0.0 0.0 0.0 0.014319586867269068 0.0 30-31 0.0 0.0 0.0 0.042806962861164775 0.0 32-33 0.0 0.0 0.0 0.10043950505840671 0.0 34-35 0.0 0.0 0.0 0.20528114459544383 0.0 36-37 0.0 0.0 0.0 0.3639097835667814 0.0 38-39 0.0 0.0 0.0 0.5672681567807545 0.0 40-41 0.0 0.0 0.0 0.7727516983637215 0.0 42-43 0.0 0.0 0.0 0.9843577488192665 0.0 44-45 0.0 0.0 0.0 1.2542541316815041 0.0 46-47 0.0 0.0 0.0 1.5664008856892173 0.0 48-49 0.0 0.0 0.0 1.892563631082915 0.0 50-51 0.0 0.0 0.0 2.200713044587044 0.0 52-53 0.0 0.0 0.0 2.5079516716473194 0.0 54-55 0.0 0.0 0.0 2.8665991335384966 0.0 56-57 0.0 0.0 0.0 3.2824743436518693 0.0 58-59 0.0 0.0 0.0 3.7072044219693834 0.0 60-61 0.0 0.0 0.0 4.1464058848948095 0.0 62-63 0.0 0.0 0.0 4.580648621625915 0.0 64-65 0.0 0.0 0.0 5.054611767158457 0.0 66-67 0.0 0.0 0.0 5.601033034224319 0.0 68-69 0.0 0.0 0.0 6.20002691879934 0.0 70-71 0.0 0.0 0.0 6.825231213258622 0.0 72-73 0.0 0.0 0.0 7.4358123253693496 0.0 74-75 0.0 0.0 0.0 8.05595669514055 0.0 76-77 5.0599246880809424E-5 0.0 0.0 8.750785553307825 0.0 78-79 1.0119849376161885E-4 0.0 0.0 9.497984631996736 0.0 80-81 1.0119849376161885E-4 0.0 0.0 10.276555243751751 0.0 82-83 1.0119849376161885E-4 0.0 0.0 11.057959413332092 0.0 84-85 1.0119849376161885E-4 5.0599246880809424E-5 0.0 11.849432833041714 0.0 86-87 1.0119849376161885E-4 1.0119849376161885E-4 0.0 12.664384303304029 0.0 88-89 1.0119849376161885E-4 1.0119849376161885E-4 0.0 13.540054869823317 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2720 0.0 47.54485 1 GTATCAA 4440 0.0 37.1578 1 TAGGGCG 60 0.003932473 31.69657 5 TATCAAC 5235 0.0 31.424103 2 TCAACGC 5230 0.0 31.090515 4 ATCAACG 5245 0.0 31.0016 3 CAACGCA 5250 0.0 30.881514 5 AACGCAG 5425 0.0 29.953241 6 ACGCAGA 6125 0.0 26.608934 7 CGCAGAG 6210 0.0 26.091692 8 GTAGCCC 770 0.0 24.08116 3 GTGTAGC 855 0.0 23.911446 1 GAGTACT 4320 0.0 23.535612 12-13 GCAGAGT 6555 0.0 23.484957 9 TAGCCCT 1210 0.0 22.397161 4 GTACTTT 4610 0.0 22.002419 14-15 CAGAGTA 6540 0.0 21.141884 10-11 AGTACTT 4310 0.0 20.448532 12-13 TAGGACC 515 0.0 20.310423 4 GTACATG 3415 0.0 20.048197 1 >>END_MODULE