FastQCFastQC Report
Thu 26 May 2016
SRR938511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938511_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988157
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45240.45782198577756367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42150.4265516512052234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.2752599030316033No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT13240.13398680574038335No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10540.10666321242474627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.041.3563461
GTATCAA39850.033.1531751
ATCAACG48550.026.9076733
TATCAAC49000.026.8653372
TCAACGC48650.026.8523674
CAACGCA49300.026.4019725
AACGCAG51200.025.5046656
ACGCAGA58300.022.3171587
CGCAGAG58550.022.0596648
GTACATG33500.021.8466341
GTATAGC7850.021.7942261
TACATGG34000.020.966262
CTCCGAC2050.020.85659294-95
CACGAGC3000.020.5862394-95
ACATGGG33700.020.4395263
GAGTACT41650.020.2937312-13
GCAGAGT61450.020.1685899
GTAGGAC5400.019.3535943
AGTACTT42700.019.29427712-13
CATGGGG12600.019.227924
Produced by FastQC (version 0.11.5)