##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938511_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 988157 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.766412624714494 31.0 31.0 34.0 30.0 34.0 2 31.991541829891403 31.0 31.0 34.0 30.0 34.0 3 32.048299005117606 33.0 31.0 34.0 30.0 34.0 4 35.73391981233751 37.0 35.0 37.0 33.0 37.0 5 35.630483819878826 37.0 35.0 37.0 33.0 37.0 6 35.62350213579421 37.0 35.0 37.0 33.0 37.0 7 35.565901977114976 37.0 35.0 37.0 33.0 37.0 8 35.55435624096171 37.0 35.0 37.0 33.0 37.0 9 37.13604214714868 39.0 37.0 39.0 33.0 39.0 10-11 37.04440134513038 39.0 37.0 39.0 33.0 39.0 12-13 36.95881524899383 39.0 37.0 39.0 33.0 39.0 14-15 37.99460814425238 40.0 37.0 41.0 33.0 41.0 16-17 37.910529399680414 40.0 37.0 41.0 33.0 41.0 18-19 38.16649884583118 40.0 37.0 41.0 33.0 41.0 20-21 38.16749514500226 40.0 37.0 41.0 33.0 41.0 22-23 38.05289139276451 40.0 37.0 41.0 33.0 41.0 24-25 37.953269065543225 40.0 37.0 41.0 33.0 41.0 26-27 37.77865814845212 40.0 37.0 41.0 32.0 41.0 28-29 37.6629913060374 40.0 37.0 41.0 32.0 41.0 30-31 37.416181335557 39.0 36.0 41.0 32.0 41.0 32-33 37.201538318303676 39.0 36.0 41.0 31.0 41.0 34-35 37.07892723524703 39.0 36.0 41.0 31.0 41.0 36-37 37.02732308732317 39.0 36.0 41.0 31.0 41.0 38-39 36.86104283023852 39.0 35.5 41.0 31.0 41.0 40-41 36.70106622733027 39.0 35.0 41.0 30.0 41.0 42-43 36.48733045457351 39.0 35.0 41.0 30.0 41.0 44-45 36.140425053913496 39.0 35.0 40.0 30.0 41.0 46-47 35.720416897314905 39.0 35.0 40.0 28.5 41.0 48-49 35.35008303336413 38.5 35.0 40.0 27.0 41.0 50-51 34.99303349568945 38.0 34.0 40.0 25.5 41.0 52-53 34.56857462933522 38.0 34.0 40.0 24.0 41.0 54-55 34.27363920915401 38.0 33.0 40.0 23.5 41.0 56-57 33.98276235456511 38.0 33.0 40.0 23.0 41.0 58-59 33.559193022971044 38.0 33.0 40.0 21.5 41.0 60-61 33.21810451173245 37.0 32.0 40.0 19.5 41.0 62-63 32.795110493575415 37.0 31.5 39.5 18.0 40.5 64-65 32.32217147679974 36.0 31.0 39.0 13.5 40.0 66-67 31.86756507316145 36.0 31.0 39.0 12.0 40.0 68-69 31.429858311988884 35.0 30.0 38.5 8.0 40.0 70-71 30.928620148417714 35.0 29.5 38.0 7.5 40.0 72-73 30.470798668632618 34.5 29.0 37.5 7.0 39.5 74-75 29.92487276819372 34.0 29.0 37.0 2.0 39.0 76-77 28.613504230603034 32.5 27.0 35.5 2.0 38.0 78-79 29.31195245289969 34.0 29.0 36.0 2.0 38.0 80-81 28.95300493747451 34.0 28.0 35.5 2.0 37.5 82-83 28.531117018854292 34.0 27.5 35.0 2.0 37.0 84-85 28.003009137212004 33.0 26.5 35.0 2.0 36.5 86-87 27.421189649013264 33.0 26.0 35.0 2.0 36.0 88-89 26.74059081704628 32.0 25.0 35.0 2.0 35.5 90-91 26.184072470265352 32.0 24.5 34.0 2.0 35.0 92-93 25.443646100771435 31.5 21.5 34.0 2.0 35.0 94-95 24.7120513238281 31.0 19.0 34.0 2.0 35.0 96-97 23.74477638674826 31.0 4.5 34.0 2.0 35.0 98-99 22.49540660036816 30.5 2.0 34.0 2.0 35.0 100-101 18.595335053033068 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32262647437201863 1101 2 0.31466511944955045 1101 3 0.23534709176833957 1101 4 0.10496845629266005 1101 5 -0.036545603382123204 1101 6 0.29483819237125175 1101 7 0.2650817279058231 1101 8 0.38105940378041225 1101 9 0.27728643616693915 1101 10-11 0.21703734828747656 1101 12-13 0.35426629024578205 1101 14-15 0.29455288811989533 1101 16-17 0.3974103503408557 1101 18-19 0.471729004731408 1101 20-21 0.3907242636135564 1101 22-23 0.4183657799427962 1101 24-25 0.5684489949835267 1101 26-27 0.41969234109382114 1101 28-29 0.45203949817855005 1101 30-31 0.4350850972713616 1101 32-33 0.2809111300371754 1101 34-35 0.5515792154435957 1101 36-37 0.3909115274726247 1101 38-39 0.5852878961003043 1101 40-41 0.5678262213718384 1101 42-43 0.5586857478028335 1101 44-45 0.5929083700794706 1101 46-47 0.6465188904780987 1101 48-49 0.6457433930520153 1101 50-51 0.7359899048941685 1101 52-53 0.6804834020383552 1101 54-55 0.6235815418224746 1101 56-57 0.7779953722579549 1101 58-59 0.814806310533065 1101 60-61 0.9923709084549799 1101 62-63 0.9950349956334534 1101 64-65 0.9057620900291639 1101 66-67 0.9906968755472754 1101 68-69 0.9628495846042178 1101 70-71 0.7189544813343431 1101 72-73 0.9826264479275828 1101 74-75 0.9622686709676529 1101 76-77 0.8132426914635253 1101 78-79 0.919940932485023 1101 80-81 1.2669133429167303 1101 82-83 0.8877480905184889 1101 84-85 1.0031348652069774 1101 86-87 0.9902920254123586 1101 88-89 1.2046965185590182 1101 90-91 1.137163604073244 1101 92-93 1.3180476960637648 1101 94-95 0.8443217869626736 1101 96-97 1.1511196021218204 1101 98-99 1.0993313787270047 1101 100-101 1.2439444993810902 1102 1 0.339507096788747 1102 2 0.3632209181366406 1102 3 0.24704414987677126 1102 4 0.24512162915917912 1102 5 0.23430049435675215 1102 6 0.20533904333162667 1102 7 0.2947656569114159 1102 8 0.310624198334267 1102 9 0.278324607817801 1102 10-11 0.4741343577696071 1102 12-13 0.5904503408171422 1102 14-15 0.4205144733374979 1102 16-17 0.4934229931806371 1102 18-19 0.3744520779140075 1102 20-21 0.5603133713208308 1102 22-23 0.4288362394613898 1102 24-25 0.5348178255208467 1102 26-27 0.6018306829367575 1102 28-29 0.7563344167294872 1102 30-31 0.568548505975464 1102 32-33 0.5219031066965485 1102 34-35 0.46607958014089945 1102 36-37 0.427056676998518 1102 38-39 0.5122134978508512 1102 40-41 0.6053110256509555 1102 42-43 0.5797616048414937 1102 44-45 0.6064082485137092 1102 46-47 0.6000576699577991 1102 48-49 0.6818508571901134 1102 50-51 0.6846210743568548 1102 52-53 0.7885541533147986 1102 54-55 0.7486204428679315 1102 56-57 0.8580926248716239 1102 58-59 0.9759502444012824 1102 60-61 0.7324839646183747 1102 62-63 0.9363381806565556 1102 64-65 0.9877448276902427 1102 66-67 0.9909047530089872 1102 68-69 1.0241928863304572 1102 70-71 0.9647543840817328 1102 72-73 1.0947368296440878 1102 74-75 0.862865558884014 1102 76-77 0.8964872817868894 1102 78-79 0.9584006942161203 1102 80-81 1.262017889476418 1102 82-83 1.4330252604674314 1102 84-85 1.4207764893256254 1102 86-87 0.9604172381524485 1102 88-89 1.188491801807256 1102 90-91 1.0852294927190016 1102 92-93 1.6637539931704985 1102 94-95 1.375530150687453 1102 96-97 1.5239727506751848 1102 98-99 1.7300194409686789 1102 100-101 2.1220164663151984 1103 1 0.11445542485768456 1103 2 0.14351373352204888 1103 3 0.0981450069543115 1103 4 0.014030289981356248 1103 5 0.03602724665341839 1103 6 -0.028987915186199587 1103 7 0.1155981931984087 1103 8 0.10460832583680002 1103 9 0.16169534400868457 1103 10-11 0.13825980742037558 1103 12-13 0.18469154492476747 1103 14-15 0.30002789404265684 1103 16-17 0.27653851902126547 1103 18-19 0.3554769317629223 1103 20-21 0.33880291796086937 1103 22-23 0.20001823196837876 1103 24-25 0.137206398442423 1103 26-27 0.11242984405520673 1103 28-29 0.26431265619797273 1103 30-31 0.1787228608344904 1103 32-33 0.26211226720845815 1103 34-35 0.2719132595089775 1103 36-37 0.5102683149637315 1103 38-39 0.530991520833787 1103 40-41 0.3940463113978936 1103 42-43 0.4799472971987129 1103 44-45 0.4891829495630091 1103 46-47 0.4784035149746657 1103 48-49 0.5030449803536001 1103 50-51 0.5939595803265405 1103 52-53 0.23969093579813006 1103 54-55 0.2639144013344321 1103 56-57 0.6026174997159117 1103 58-59 0.6043929013883087 1103 60-61 0.43111646637964895 1103 62-63 0.202417132567831 1103 64-65 0.36573958351412017 1103 66-67 0.4328397326901303 1103 68-69 0.47675268813418725 1103 70-71 0.19208288692543718 1103 72-73 0.06889036643030622 1103 74-75 0.2621111253623454 1103 76-77 0.44781862133796224 1103 78-79 0.41055808481859657 1103 80-81 0.5219370339851963 1103 82-83 0.4902889576115932 1103 84-85 0.53995552381868 1103 86-87 0.5548205731498612 1103 88-89 0.7773570255478823 1103 90-91 0.45716241951982184 1103 92-93 0.8552142442750927 1103 94-95 0.5640848762780024 1103 96-97 0.8848947738820634 1103 98-99 0.7220132076774881 1103 100-101 1.0108646604803546 1104 1 0.24042495608354741 1104 2 0.3159280594081437 1104 3 0.23088194339014478 1104 4 0.16283595111667637 1104 5 0.1479057423735739 1104 6 0.1795655905769138 1104 7 0.20301823584232892 1104 8 0.09010012151678382 1104 9 0.21877021808550268 1104 10-11 0.1922754435255669 1104 12-13 0.26749955456600105 1104 14-15 0.34568470316474986 1104 16-17 0.3732212544126341 1104 18-19 0.347643428471919 1104 20-21 0.3444578729993424 1104 22-23 0.3312332747668094 1104 24-25 0.36970848428649106 1104 26-27 -0.04538357332992149 1104 28-29 0.07953777285074182 1104 30-31 0.2787738481340156 1104 32-33 0.3004763474284786 1104 34-35 0.337686875899081 1104 36-37 0.10988668275211921 1104 38-39 0.2643665710485479 1104 40-41 0.42757432351124436 1104 42-43 0.540228190867019 1104 44-45 0.2667006406108783 1104 46-47 0.24162931145808386 1104 48-49 0.30635760975318505 1104 50-51 0.5086331071025967 1104 52-53 0.3291272943779049 1104 54-55 0.30960983719859314 1104 56-57 0.29136321214753735 1104 58-59 0.6126358481452101 1104 60-61 0.6512425440657523 1104 62-63 0.23440007499853976 1104 64-65 0.2886019796081811 1104 66-67 0.405407020474815 1104 68-69 0.5059931318782027 1104 70-71 0.49029678637230134 1104 72-73 0.6838203747736848 1104 74-75 0.7785661174824909 1104 76-77 0.43684862659119617 1104 78-79 0.5410623955630101 1104 80-81 0.4690492987483381 1104 82-83 0.8861435356530656 1104 84-85 0.8556055346341687 1104 86-87 0.7728144546739166 1104 88-89 0.7771947932312067 1104 90-91 0.9391546323686235 1104 92-93 1.1573989728063445 1104 94-95 1.2792976323733036 1104 96-97 0.8916544537436266 1104 98-99 1.2514776865254866 1104 100-101 1.6590445683942008 1105 1 0.2524042521497982 1105 2 0.2676651194495534 1105 3 0.2835693139905615 1105 4 0.2529684562926562 1105 5 0.2271210632845424 1105 6 0.2703937479268106 1105 7 0.33930395012804127 1105 8 0.3855038482248574 1105 9 0.36306421394471755 1105 10-11 0.3518706816208095 1105 12-13 0.4452107346902281 1105 14-15 0.6033306658976656 1105 16-17 0.47052146145196616 1105 18-19 0.4610067825091946 1105 20-21 0.46611315250245156 1105 22-23 0.37636577994279463 1105 24-25 0.5011712172057514 1105 26-27 0.4600812299827126 1105 28-29 0.5706506092896575 1105 30-31 0.6151962083824642 1105 32-33 0.5732444633705072 1105 34-35 0.5622458821102612 1105 36-37 0.4882448608059491 1105 38-39 0.5097878961002991 1105 40-41 0.5780484435940636 1105 42-43 0.5573524144695057 1105 44-45 0.49318614785725856 1105 46-47 0.5169077793669814 1105 48-49 0.663687837496461 1105 50-51 0.6312676826719468 1105 52-53 0.557816735371695 1105 54-55 0.38469265293358035 1105 56-57 0.31977315003573636 1105 58-59 0.4724174216441739 1105 60-61 0.6470375751216508 1105 62-63 0.8192572178556716 1105 64-65 0.7377065344736096 1105 66-67 0.4481968755472785 1105 68-69 0.4714051401597743 1105 70-71 0.7401767035565676 1105 72-73 0.8854042257053614 1105 74-75 0.6838797820787619 1105 76-77 0.6743538025746361 1105 78-79 0.9763853769294677 1105 80-81 1.1465244540278405 1105 82-83 1.121414757185157 1105 84-85 0.9625237540958658 1105 86-87 1.1871809143012477 1105 88-89 1.3324742963367946 1105 90-91 1.4456080485176876 1105 92-93 1.3086588071748757 1105 94-95 1.469710675851566 1105 96-97 1.5466751576773738 1105 98-99 1.871109156504783 1105 100-101 2.122500054936644 1106 1 -0.01659574785020368 1106 2 0.13166511944955062 1106 3 0.17556931399055742 1106 4 0.07496845629265891 1106 5 0.01712106328454155 1106 6 0.10839374792681156 1106 7 0.0743039501280407 1106 8 0.12250384822485927 1106 9 -0.16193578605528103 1106 10-11 0.16237068162080703 1106 12-13 0.19571073469022338 1106 14-15 0.029830665897669917 1106 16-17 0.20052146145196303 1106 18-19 0.12450678250919367 1106 20-21 0.1761131525024453 1106 22-23 0.1688657799427915 1106 24-25 0.38167121720574926 1106 26-27 0.32658122998271466 1106 28-29 0.18415060928965943 1106 30-31 0.033196208382470616 1106 32-33 0.05874446337050898 1106 34-35 -0.0917541178897352 1106 36-37 -0.006755139194048354 1106 38-39 0.033787896100299974 1106 40-41 -0.04545155640594345 1106 42-43 0.05535241446950323 1106 44-45 -0.19131385214274133 1106 46-47 -0.7825922206330134 1106 48-49 -0.5368121625035442 1106 50-51 -0.3617323173280482 1106 52-53 -0.3391832646283106 1106 54-55 -0.19080734706641778 1106 56-57 -0.1987268499642667 1106 58-59 -0.39108257835582805 1106 60-61 -0.3834624248783456 1106 62-63 -0.2587427821443171 1106 64-65 -0.1382934655263952 1106 66-67 -0.2583031244527234 1106 68-69 -0.1375948598402239 1106 70-71 -0.13882329644343372 1106 72-73 0.11490422570535941 1106 74-75 0.03937978207876114 1106 76-77 -0.25064619742536465 1106 78-79 0.16488537692947247 1106 80-81 -0.5184755459721586 1106 82-83 0.08641475718515679 1106 84-85 0.04352375409586884 1106 86-87 0.30068091430124966 1106 88-89 0.20097429633679553 1106 90-91 0.2526080485176898 1106 92-93 0.3946588071748742 1106 94-95 0.503210675851566 1106 96-97 0.5961751576773757 1106 98-99 0.7671091565047838 1106 100-101 0.9225000549366449 1107 1 0.15017480664310412 1107 2 0.1079920792965865 1107 3 0.1656190271836735 1107 4 0.09244455571904808 1107 5 0.056105766917433186 1107 6 0.1060151628407695 1107 7 0.1782982139903737 1107 8 0.18458606619808648 1107 9 0.28483476843802435 1107 10-11 0.16411829156344737 1107 12-13 0.3105176180554281 1107 14-15 0.3112025588231049 1107 16-17 0.2272595111651654 1107 18-19 0.2330631878629177 1107 20-21 0.19150607793265806 1107 22-23 0.11935048357567979 1107 24-25 0.1404322114696086 1107 26-27 0.18732883992534966 1107 28-29 0.16470892668927206 1107 30-31 0.1511388470057895 1107 32-33 0.3636708496037784 1107 34-35 0.5158367042899954 1107 36-37 0.5468356829856873 1107 38-39 0.4277257546089075 1107 40-41 0.5465972007642321 1107 42-43 0.6519967739341297 1107 44-45 0.7670054595207318 1107 46-47 0.6424919093861021 1107 48-49 0.7746237839591785 1107 50-51 0.8796759044692735 1107 52-53 0.9466943644347907 1107 54-55 0.6634402628762217 1107 56-57 0.6809930353129872 1107 58-59 0.9325809015676896 1107 60-61 0.6882631965365675 1107 62-63 0.7663585562878055 1107 64-65 0.7208470698464566 1107 66-67 0.8013182904612357 1107 68-69 0.8566556181712492 1107 70-71 1.0658898966732053 1107 72-73 0.9976126387072703 1107 74-75 1.0585709866676716 1107 76-77 1.0483212977945193 1107 78-79 1.3770718014036554 1107 80-81 1.1426802857678027 1107 82-83 1.0232962103400318 1107 84-85 1.1223488018970151 1107 86-87 1.1738749869589924 1107 88-89 1.2623022122067766 1107 90-91 1.3493805150568896 1107 92-93 1.4252199926433278 1107 94-95 1.6076083813964956 1107 96-97 1.7209103393217333 1107 98-99 1.8559236880535401 1107 100-101 2.2602266323745006 1108 1 0.16352925214979663 1108 2 0.12431355694955215 1108 3 0.10530368899055986 1108 4 0.14266376879265863 1108 5 0.14918356328454507 1108 6 0.18703437292681002 1108 7 0.07648363762804422 1108 8 0.10616791072485654 1108 9 0.09897046394471687 1108 10-11 0.07099568162080772 1108 12-13 0.18826151594022633 1108 14-15 0.2928775408976705 1108 16-17 0.24016208645196713 1108 18-19 0.18671381375919083 1108 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-1.1324719266728565 2316 56-57 -0.627285883953526 2316 58-59 -0.6577051186062732 2316 60-61 -0.5696654660232454 2316 62-63 -0.6844601345593517 2316 64-65 -0.8601959699986637 2316 66-67 -0.814285235365066 2316 68-69 -0.8168095288921045 2316 70-71 -0.7521596112913755 2316 72-73 -0.7610984576577877 2316 74-75 -0.6624610050410951 2316 76-77 -0.8640093459047904 2316 78-79 -0.9876629236071999 2316 80-81 -0.874310071911335 2316 82-83 -1.0315870317236104 2316 84-85 -0.9815361743477844 2316 86-87 -1.2972502127112726 2316 88-89 -1.5026777609082842 2316 90-91 -1.2024080516611981 2316 92-93 -1.4321649853117044 2316 94-95 -1.1997410236117645 2316 96-97 -1.2026540015355067 2316 98-99 -1.4223362817778629 2316 100-101 -1.8786215908594208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 11.0 5 37.0 6 78.0 7 222.0 8 423.0 9 815.0 10 1437.0 11 2297.0 12 3288.0 13 4644.0 14 5844.0 15 7904.0 16 10044.0 17 12544.0 18 11676.0 19 8783.0 20 8160.0 21 8507.0 22 9067.0 23 10085.0 24 11523.0 25 13588.0 26 16058.0 27 19110.0 28 22891.0 29 27536.0 30 32865.0 31 39660.0 32 47506.0 33 59156.0 34 74134.0 35 96929.0 36 133541.0 37 177686.0 38 105542.0 39 4561.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.28983307274668 21.34442471069822 15.620221523951203 22.745520692603893 2 15.299304954414527 22.30848279597653 40.121898745070645 22.270313504538297 3 18.0672889705674 25.73348742506244 31.838697533587812 24.360526070782345 4 12.320511821502048 19.12752730588358 38.2114380609559 30.34052281165847 5 12.382454662530474 38.54960595482047 35.67657921806533 13.391360164583721 6 29.58945753504507 38.37602564777222 18.32119624836564 13.71332056881707 7 25.88890226957862 33.98741293134593 22.185037397903372 17.93864740117208 8 24.16427753889311 36.50715422751647 21.21990736289881 18.1086608706916 9 25.01039814523401 16.71819356640696 21.887817421725494 36.38359086663354 10-11 23.001911639547156 27.84552454721264 29.30339004834252 19.849173764897685 12-13 24.213156411379973 25.834103285206705 28.934268542347013 21.01847176106631 14-15 21.09801377716294 27.8142542126403 27.7319798372121 23.355752172984655 16-17 19.931599938066523 30.383582770754042 28.870867686005365 20.813949605174077 18-19 19.609181536942007 28.953192660680436 31.458310774502436 19.979315027875124 20-21 21.081923216654843 28.095282429816315 31.24361816998716 19.579176183541687 22-23 20.208580215492074 28.748265710813158 30.783974611321884 20.25917946237288 24-25 19.897145413290637 28.460601926537354 31.53014794139019 20.11210471878182 26-27 20.000091079934787 28.796692381568256 31.157433691277475 20.04578284721948 28-29 19.698701572068796 28.915460253512475 31.19161792691745 20.19422024750128 30-31 20.379116703216855 28.563187972542327 31.314066138468373 19.743629185772445 32-33 19.824399751658017 28.582097821846503 31.289571913675356 20.303930512820124 34-35 20.164214414938705 29.003959869944207 31.125691079538985 19.706134635578103 36-37 20.145476041294465 28.731518155656932 30.837641166521074 20.28536463652752 38-39 19.87040982071925 28.804390177256696 31.125931698233504 20.199268303790554 40-41 20.581410293386927 28.43579257783998 31.051238224194933 19.931558904578157 42-43 20.16433856233359 28.516064982497475 30.924167858295814 20.395428596873124 44-45 20.368870066589313 28.71037166844707 30.66088840736728 20.25986985759634 46-47 20.41851649080055 28.536710259604497 30.162818256613065 20.881954992981886 48-49 20.35602642090275 28.498204232728202 30.30444554863245 20.841323797736592 50-51 20.477923634025014 28.924115227503535 29.682654294719573 20.915306843751882 52-53 20.396505818407398 28.922883711798836 29.85274607172747 20.827864398066296 54-55 20.546448695736654 28.678512443530497 29.681554510273806 21.093484350459043 56-57 20.592527300823654 28.92354150200828 29.314572481903177 21.16935871526488 58-59 20.3827307078357 28.97967157492406 29.3595579430464 21.27803977419384 60-61 20.515414048577302 28.88523787211951 29.31948060885062 21.279867470452572 62-63 20.256005424744323 28.81784093151766 29.364363680527088 21.561789963210924 64-65 20.27241971766123 28.74784602187759 29.240509211814807 21.73922504864638 66-67 20.184132931143452 28.916227815164703 29.018343967480714 21.88129528621113 68-69 20.39375565853592 28.53698664522264 29.117200076720763 21.952057619520676 70-71 20.046510827732842 28.312150801947467 29.288716266747084 22.35262210357261 72-73 20.05871539579004 28.05446303293051 29.33867256856581 22.54814900271364 74-75 20.082273160456452 28.092620063091612 28.946781067658232 22.878325708793703 76-77 19.675369414985575 28.051745687985896 29.46339211270087 22.809492784327663 78-79 19.684263805233588 27.93749050969277 29.260262185554488 23.117983499519156 80-81 19.40064328861693 28.014434768965245 29.22823080446506 23.35669113795276 82-83 19.580332150576275 27.958908893961866 29.337166748497935 23.123592206963924 84-85 19.575539014945253 27.685712434944726 29.261866830768234 23.476881719341787 86-87 19.195654154057912 27.74570627587114 29.186494883965715 23.872144686105234 88-89 19.63871267017478 27.397006372233008 29.107527823763167 23.85675313382905 90-91 19.361225620819763 27.377512570761937 29.21245157061769 24.048810237800602 92-93 19.130815030458116 27.178276651611377 29.33590373251113 24.35500458541938 94-95 19.00500262177062 27.264642943185525 29.167906693309526 24.562447741734335 96-97 19.03269600289947 27.09072796593525 29.16429599723011 24.712280033935162 98-99 19.123160869437548 26.768000469812996 29.08365202652418 25.02518663422527 100-101 18.816070007069737 26.817116550414998 29.32239354786797 25.044419894647298 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 370.0 1 221.5 2 87.0 3 106.5 4 88.0 5 83.0 6 149.0 7 260.0 8 369.5 9 428.5 10 434.5 11 437.0 12 473.5 13 554.5 14 670.5 15 805.5 16 967.5 17 1137.0 18 1380.0 19 1673.5 20 1997.0 21 2469.0 22 2981.5 23 3616.0 24 4547.5 25 5709.0 26 7382.0 27 9430.0 28 11482.5 29 13789.5 30 16368.5 31 19032.5 32 21783.0 33 24635.0 34 27933.5 35 31113.5 36 34007.5 37 36267.5 38 37157.5 39 37597.0 40 37956.5 41 38090.0 42 38559.0 43 39115.0 44 39323.0 45 39533.0 46 39795.0 47 39985.0 48 39781.5 49 38375.5 50 36338.0 51 33759.0 52 30080.5 53 26677.5 54 23500.0 55 19869.0 56 16294.5 57 13183.5 58 10472.5 59 7989.0 60 5898.0 61 4258.5 62 2938.5 63 2027.0 64 1394.5 65 928.5 66 632.0 67 440.0 68 333.0 69 238.0 70 167.0 71 116.5 72 83.5 73 73.0 74 53.0 75 33.5 76 20.5 77 13.0 78 8.0 79 6.0 80 3.5 81 1.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.0239698752323772E-4 2 0.04574171918025172 3 1.0119849376161886E-4 4 0.0 5 0.045842917674013335 6 1.0119849376161886E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006375505106981987 26-27 0.001416778912662664 28-29 5.059924688080943E-5 30-31 0.0018215728877091395 32-33 3.54194728165666E-4 34-35 0.012093220004513452 36-37 0.0230732565776491 38-39 0.004250336737987991 40-41 8.095879500929509E-4 42-43 0.001416778912662664 44-45 0.008652471216618413 46-47 0.0 48-49 0.0 50-51 7.589887032121414E-4 52-53 0.0 54-55 0.001315580418901045 56-57 0.0 58-59 0.023275653565172336 60-61 0.0 62-63 0.009310261426068934 64-65 0.016141159754978207 66-67 0.006729699835147654 68-69 0.01603996126121659 70-71 0.0 72-73 5.059924688080943E-5 74-75 0.023123855824529905 76-77 0.04862587625245786 78-79 0.031067937584816987 80-81 0.026767001599948187 82-83 0.03562186980408984 84-85 0.015280972558004445 86-87 0.00880426895726084 88-89 0.08364055509397798 90-91 0.025249024193523907 92-93 0.024591233984073382 94-95 0.027829585784445186 96-97 0.03956861106079297 98-99 0.053635201693657986 100-101 0.014420785361030685 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 988157.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.91912810736996 #Duplication Level Percentage of deduplicated Percentage of total 1 76.6364796834826 52.0508288132031 2 14.605081708060128 19.839288310966825 3 4.7213191038815125 9.620036311569047 4 1.8827882948507921 5.115093575881104 5 0.8885286771373735 3.0174046524782607 6 0.4847694536957799 1.9755071176862051 7 0.2637914691821422 1.2541540612309274 8 0.1450307749070854 0.7880291024340375 9 0.09287531830596281 0.5677209577831904 >10 0.2596453173428713 2.7341250986880237 >50 0.01044496431544454 0.5005779595099185 >100 0.008052302186328514 0.9391577150589343 >500 4.4734974448416517E-4 0.19668320047260715 >1k 7.455829074736086E-4 1.4013931230378296 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4524 0.45782198577756367 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4215 0.4265516512052234 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2720 0.2752599030316033 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1324 0.13398680574038335 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1054 0.10666321242474627 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.0599246880809424E-5 0.0 12-13 0.0 0.0 0.0 1.0119849376161885E-4 0.0 14-15 0.0 0.0 0.0 1.0119849376161885E-4 0.0 16-17 0.0 0.0 0.0 1.0119849376161885E-4 0.0 18-19 0.0 0.0 0.0 1.0119849376161885E-4 0.0 20-21 0.0 0.0 0.0 1.5179774064242826E-4 0.0 22-23 0.0 0.0 0.0 9.107864438545697E-4 0.0 24-25 0.0 0.0 0.0 0.002276966109636424 0.0 26-27 0.0 0.0 0.0 0.004047939750464754 0.0 28-29 0.0 0.0 0.0 0.014623182348553924 0.0 30-31 0.0 0.0 0.0 0.0434647530706153 0.0 32-33 0.0 0.0 0.0 0.1020080817117118 0.0 34-35 0.0 0.0 0.0 0.2083676986551732 0.0 36-37 0.0 0.0 0.0 0.36805892181100774 0.0 38-39 0.0 0.0 0.0 0.5718220890000274 0.0 40-41 0.0 0.0 0.0 0.779026004976942 0.0 42-43 0.0 0.0 0.0 0.9917958381107457 0.0 44-45 0.0 0.0 0.0 1.2641209848232617 0.0 46-47 0.0 0.0 0.0 1.5780387124718036 0.0 48-49 0.0 0.0 0.0 1.9054664390375213 0.0 50-51 0.0 0.0 0.0 2.213868848776055 0.0 52-53 0.0 0.0 0.0 2.5210062773425683 0.0 54-55 0.0 0.0 0.0 2.8814247128745736 0.0 56-57 0.0 0.0 0.0 3.2983625071724436 0.0 58-59 0.0 0.0 0.0 3.7249141583776666 0.0 60-61 0.0 0.0 0.0 4.1646216137719 0.0 62-63 0.0 0.0 0.0 4.598510155774841 0.0 64-65 0.0 0.0 0.0 5.070904724654079 0.0 66-67 0.0 0.0 0.0 5.617224793226178 0.0 68-69 0.0 0.0 0.0 6.220064220564141 0.0 70-71 0.0 0.0 0.0 6.847950275108105 0.0 72-73 0.0 0.0 0.0 7.4644514991038875 0.0 74-75 0.0 0.0 0.0 8.092084557413447 0.0 76-77 0.0 0.0 0.0 8.796021280019268 0.0 78-79 0.0 0.0 0.0 9.548634478124427 0.0 80-81 0.0 0.0 0.0 10.336363553564869 0.0 82-83 0.0 0.0 0.0 11.129304351434033 0.0 84-85 0.0 0.0 0.0 11.935299754998447 0.0 86-87 0.0 0.0 0.0 12.768466954137853 0.0 88-89 0.0 0.0 0.0 13.667362574975435 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2620 0.0 41.356346 1 GTATCAA 3985 0.0 33.153175 1 ATCAACG 4855 0.0 26.907673 3 TATCAAC 4900 0.0 26.865337 2 TCAACGC 4865 0.0 26.852367 4 CAACGCA 4930 0.0 26.401972 5 AACGCAG 5120 0.0 25.504665 6 ACGCAGA 5830 0.0 22.317158 7 CGCAGAG 5855 0.0 22.059664 8 GTACATG 3350 0.0 21.846634 1 GTATAGC 785 0.0 21.794226 1 TACATGG 3400 0.0 20.96626 2 CTCCGAC 205 0.0 20.856592 94-95 CACGAGC 300 0.0 20.58623 94-95 ACATGGG 3370 0.0 20.439526 3 GAGTACT 4165 0.0 20.29373 12-13 GCAGAGT 6145 0.0 20.168589 9 GTAGGAC 540 0.0 19.353594 3 AGTACTT 4270 0.0 19.294277 12-13 CATGGGG 1260 0.0 19.22792 4 >>END_MODULE