FastQCFastQC Report
Thu 26 May 2016
SRR938510_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938510_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974164
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49820.5114128627212666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42020.4313442089832923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26930.2764421596363651No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10530.10809268254626532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25200.042.145521
GTATCAA41450.036.193711
ATCAACG49550.030.315433
TCAACGC49650.030.1586344
TATCAAC49950.030.0726682
CAACGCA50600.029.6848325
GCCGTAA650.0057682829.3085081
GACCGTG650.00583536829.2392437
AACGCAG51300.029.178126
ACGCAGA59000.025.1259237
CGCAGAG59100.025.0834128
TCGAGAC1351.3346496E-524.6481673
GTATAGC7250.024.305851
GTAGCCC6100.024.1575133
TAGCCCT10650.024.1026334
GTACATG34150.023.429641
GCAGAGT63100.022.6638839
TACATGG34500.022.5967042
GCCGTAT1406.548362E-1122.067994-95
ACATGGG34950.021.4896933
Produced by FastQC (version 0.11.5)