FastQCFastQC Report
Thu 26 May 2016
SRR938510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938510_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974164
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43800.44961628637477874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41490.42590364661391716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26800.27510768207406555No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT13300.13652731983526387No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.10439720621989727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23000.048.6128851
ATTACCG459.582677E-442.2594645
GTATCAA39300.038.377621
TATCAAC49350.030.4657062
ATCAACG49250.030.1179123
GTATACG953.451146E-530.049621
TCAACGC49550.029.9355644
CAACGCA49650.029.8752735
AACGCAG50500.029.3437756
ACGCAGA57450.025.7939197
CGCAGAG57550.025.74918
GCAGAGT63600.022.8516259
TAGGACG4200.022.6389984
GTACATG34400.022.2678741
GGACGTG4350.021.8370276
AGTACTT43200.021.6038712-13
GTGGTAT10550.021.1961381
GAGTACT42500.021.06566612-13
TACATGG36200.020.8977682
AGAGTAC59450.020.69191610-11
Produced by FastQC (version 0.11.5)