##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938510_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 974164 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75958052237611 31.0 31.0 34.0 30.0 34.0 2 31.9850866999807 31.0 31.0 34.0 30.0 34.0 3 32.04638746658674 33.0 31.0 34.0 30.0 34.0 4 35.72889677713403 37.0 35.0 37.0 33.0 37.0 5 35.59867537704124 37.0 35.0 37.0 33.0 37.0 6 35.61905490245996 37.0 35.0 37.0 33.0 37.0 7 35.56047544355981 37.0 35.0 37.0 33.0 37.0 8 35.563464673299364 37.0 35.0 37.0 33.0 37.0 9 37.14106454354708 39.0 37.0 39.0 33.0 39.0 10-11 37.057459524269014 39.0 37.0 39.0 33.0 39.0 12-13 36.9704639054615 39.0 37.0 39.0 33.0 39.0 14-15 38.017063861937004 40.0 37.0 41.0 33.0 41.0 16-17 37.94682979457258 40.0 37.0 41.0 33.0 41.0 18-19 38.08886748021894 40.0 37.0 41.0 33.0 41.0 20-21 38.160212756784276 40.0 37.0 41.0 33.0 41.0 22-23 38.05265386526293 40.0 37.0 41.0 33.0 41.0 24-25 37.97846871779289 40.0 37.0 41.0 33.0 41.0 26-27 37.80160065450992 40.0 37.0 41.0 32.0 41.0 28-29 37.67949955038371 40.0 37.0 41.0 32.0 41.0 30-31 37.45357917147421 39.0 36.5 41.0 32.0 41.0 32-33 37.22675237434354 39.0 36.0 41.0 31.5 41.0 34-35 37.06543046140075 39.0 36.0 41.0 31.0 41.0 36-37 37.04134930052845 39.0 36.0 41.0 31.0 41.0 38-39 36.91790550667034 39.0 36.0 41.0 31.0 41.0 40-41 36.745476634324405 39.0 35.0 41.0 30.0 41.0 42-43 36.52374856800292 39.0 35.0 41.0 30.0 41.0 44-45 36.217609663259985 39.0 35.0 40.5 30.0 41.0 46-47 35.80116746256277 39.0 35.0 40.0 28.5 41.0 48-49 35.44041814314633 39.0 35.0 40.0 27.0 41.0 50-51 35.107077966338316 38.5 34.5 40.0 26.0 41.0 52-53 34.67277378346972 38.0 34.0 40.0 24.0 41.0 54-55 34.41624510862647 38.0 34.0 40.0 24.0 41.0 56-57 34.110855564360826 38.0 33.0 40.0 23.0 41.0 58-59 33.72291267178832 38.0 33.0 40.0 22.0 41.0 60-61 33.409986922119884 37.5 32.5 40.0 21.0 41.0 62-63 33.002777766371985 37.0 32.0 39.5 18.5 41.0 64-65 32.56394200565819 36.5 31.0 39.0 17.5 40.0 66-67 32.103041684972965 36.0 31.0 39.0 13.0 40.0 68-69 31.617138387376254 35.5 30.5 38.5 10.0 40.0 70-71 31.09083121527792 35.0 29.5 38.0 8.0 40.0 72-73 30.695125256117038 35.0 29.0 37.5 7.5 39.5 74-75 30.22335869525049 34.0 29.0 37.0 6.5 39.0 76-77 28.865798777207946 32.5 27.0 35.5 2.0 38.0 78-79 29.588988609720744 34.0 29.0 36.0 2.0 38.5 80-81 29.329432210592877 34.0 29.0 36.0 2.0 37.5 82-83 28.87956186022066 34.0 28.5 35.0 2.0 37.0 84-85 28.386883009431678 33.5 27.5 35.0 2.0 36.5 86-87 27.84865997922321 33.0 26.5 35.0 2.0 36.0 88-89 27.263636307644298 33.0 26.0 35.0 2.0 35.5 90-91 26.77422384731934 32.0 25.5 34.5 2.0 35.0 92-93 26.12274781248332 32.0 24.5 34.0 2.0 35.0 94-95 25.454164288559216 32.0 21.5 34.0 2.0 35.0 96-97 24.57327667620647 31.0 17.5 34.0 2.0 35.0 98-99 23.45670184896999 31.0 2.0 34.0 2.0 35.0 100-101 19.876817969048332 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3801994760242273 1101 2 0.3782605795773506 1101 3 0.32614666567769035 1101 4 0.27621615892413587 1101 5 0.1671240620777681 1101 6 0.15683654093301413 1101 7 0.32559781332646764 1101 8 0.26775693309581783 1101 9 0.4362538362994641 1101 10-11 0.40611895068482795 1101 12-13 0.38647326617434175 1101 14-15 0.4154913234400155 1101 16-17 0.44764381575459566 1101 18-19 0.34100051296648104 1101 20-21 0.3598516375440255 1101 22-23 0.4016234683026454 1101 24-25 0.4721129433717195 1101 26-27 0.4448078939677913 1101 28-29 0.4195071437874631 1101 30-31 0.29141859092808886 1101 32-33 0.2839313417980094 1101 34-35 0.10715029351079153 1101 36-37 0.3606762562871282 1101 38-39 0.47030974075808984 1101 40-41 0.48145817852478245 1101 42-43 0.7086498143311886 1101 44-45 0.6415508551478908 1101 46-47 0.5690722725361752 1101 48-49 0.6887320542239905 1101 50-51 0.77174740549777 1101 52-53 0.7624558595977291 1101 54-55 0.8292895373087035 1101 56-57 0.9139939692474996 1101 58-59 0.7593241243071347 1101 60-61 0.6314882958494934 1101 62-63 0.5065426196907055 1101 64-65 0.6878713329877328 1101 66-67 0.6504309048124881 1101 68-69 0.7760798586223636 1101 70-71 0.8439727457710688 1101 72-73 0.7402992354857183 1101 74-75 0.8025966866701246 1101 76-77 0.8127950297923938 1101 78-79 0.8124796416448774 1101 80-81 0.6843411112701219 1101 82-83 0.3949750576040074 1101 84-85 0.6463984480767628 1101 86-87 0.7537545935763674 1101 88-89 0.7223760567946904 1101 90-91 0.6862966349376123 1101 92-93 0.7614497787235344 1101 94-95 0.4481739808217995 1101 96-97 0.678398708911196 1101 98-99 0.1297224569569302 1101 100-101 -0.06551620240744072 1102 1 0.4176576700041643 1102 2 0.28054056759711443 1102 3 0.4292690138032853 1102 4 0.35663721418296035 1102 5 0.2344829199622609 1102 6 0.30631490163784747 1102 7 0.304341636019025 1102 8 0.30595765190977176 1102 9 0.22196990478646228 1102 10-11 0.3393154264997307 1102 12-13 0.3747825558463802 1102 14-15 0.5013883433571564 1102 16-17 0.5836600140274513 1102 18-19 0.4675055296888857 1102 20-21 0.47576849972143265 1102 22-23 0.4604482823594509 1102 24-25 0.5016579441204811 1102 26-27 0.38547516796569425 1102 28-29 0.444027310512368 1102 30-31 0.3889133495763133 1102 32-33 0.40639365398290295 1102 34-35 0.47733573752817193 1102 36-37 0.5317196596715448 1102 38-39 0.4596105194732161 1102 40-41 0.6947512449651541 1102 42-43 0.5580501787299355 1102 44-45 0.8688545540447095 1102 46-47 1.1069703656824785 1102 48-49 1.0303072052248368 1102 50-51 0.9789424087424194 1102 52-53 0.8051566532881367 1102 54-55 0.8593387061800684 1102 56-57 0.9204882536781867 1102 58-59 1.103919916250426 1102 60-61 1.3537365861704629 1102 62-63 1.2225580691990103 1102 64-65 0.9990890737155311 1102 66-67 1.092949499131862 1102 68-69 1.1574936508651597 1102 70-71 0.9896898126107843 1102 72-73 0.875292746193054 1102 74-75 1.0031364211083016 1102 76-77 0.7990707997719291 1102 78-79 0.8803638826472238 1102 80-81 0.9952474907439885 1102 82-83 1.056419923575156 1102 84-85 0.7896995013388839 1102 86-87 0.6319125080175425 1102 88-89 0.9250285551723678 1102 90-91 0.9343061692060708 1102 92-93 1.2386786011665016 1102 94-95 1.3101036718316337 1102 96-97 1.1104246161642308 1102 98-99 1.0174626855797015 1102 100-101 0.4734420165313118 1103 1 0.25753372306894207 1103 2 0.20630555258792072 1103 3 0.2821159199715808 1103 4 0.1965302131177893 1103 5 0.30080421365839527 1103 6 0.31362583414487233 1103 7 0.33277922680047567 1103 8 0.28661757250626607 1103 9 0.40612309545171144 1103 10-11 0.3783611319320954 1103 12-13 0.4587904961969187 1103 14-15 0.6199634377698615 1103 16-17 0.5548580870399391 1103 18-19 0.4913774189113127 1103 20-21 0.5803160449911076 1103 22-23 0.4922036324127035 1103 24-25 0.5986730501532094 1103 26-27 0.5138655523948614 1103 28-29 0.3008552857229816 1103 30-31 0.2960608754792844 1103 32-33 0.600427497306228 1103 34-35 0.5509461761841195 1103 36-37 0.625600990164429 1103 38-39 0.4513954037571324 1103 40-41 0.17351080727753754 1103 42-43 0.3259195986970056 1103 44-45 0.33455853390333345 1103 46-47 0.4039067944614061 1103 48-49 0.37891038136505273 1103 50-51 0.2927060864803863 1103 52-53 0.3343204956431265 1103 54-55 0.43832824815740423 1103 56-57 0.5085196277461677 1103 58-59 0.18194717489281942 1103 60-61 0.6600017164115783 1103 62-63 0.6410665905508068 1103 64-65 0.6302864204764447 1103 66-67 0.36969734458552495 1103 68-69 0.4447612503616263 1103 70-71 0.28930732011538396 1103 72-73 0.4548971811693292 1103 74-75 0.5855817101564291 1103 76-77 0.4001226541342824 1103 78-79 0.4167418239660954 1103 80-81 0.3762281724521337 1103 82-83 0.4985110439392457 1103 84-85 0.11600433333268612 1103 86-87 0.238515345240355 1103 88-89 0.5949702129626253 1103 90-91 0.5043289250887106 1103 92-93 0.4109285285413442 1103 94-95 0.7286460849235254 1103 96-97 0.4259107205507888 1103 98-99 0.3894826332895889 1103 100-101 0.01861002272865875 1104 1 0.31427705099163106 1104 2 0.2912259028955688 1104 3 0.25252804106931137 1104 4 0.18457568139003655 1104 5 0.18655550655032016 1104 6 0.18085432684050318 1104 7 0.11121341199544332 1104 8 0.1508544831551646 1104 9 0.3245859696353932 1104 10-11 0.32460466747246386 1104 12-13 0.3751418041883099 1104 14-15 0.49972566833872634 1104 16-17 0.3784278843295894 1104 18-19 0.5770256107500487 1104 20-21 0.3749302535902572 1104 22-23 0.40207042679571003 1104 24-25 0.3728121445626371 1104 26-27 0.4615022497637824 1104 28-29 0.5915448123546625 1104 30-31 0.4801670341746984 1104 32-33 0.3570476701951364 1104 34-35 0.39179778616486516 1104 36-37 0.5333408829582069 1104 38-39 0.5597671149043109 1104 40-41 0.6435076930123174 1104 42-43 0.7404884617070877 1104 44-45 0.5871301620034401 1104 46-47 0.5926418837370093 1104 48-49 0.6219339554090624 1104 50-51 0.9906317970990841 1104 52-53 1.0385667490876926 1104 54-55 1.0208668933576632 1104 56-57 0.9838909802809894 1104 58-59 1.1460210584759238 1104 60-61 0.9966667630311861 1104 62-63 1.112066175315448 1104 64-65 1.1585861260368233 1104 66-67 0.8785104792350253 1104 68-69 0.9127810495387045 1104 70-71 0.8723768059785044 1104 72-73 0.5483168803124343 1104 74-75 0.5941966642917968 1104 76-77 0.6082546612310331 1104 78-79 0.7662933225882682 1104 80-81 1.0827428849960157 1104 82-83 0.6263858410401539 1104 84-85 0.5656759199092569 1104 86-87 0.8757730459689057 1104 88-89 0.9106033525194555 1104 90-91 0.8751223077469668 1104 92-93 0.9002574515718074 1104 94-95 0.9436058825906599 1104 96-97 0.7823065247861862 1104 98-99 0.48660877990542417 1104 100-101 0.06471412412158628 1105 1 0.24342159527044416 1105 2 0.4051476917458885 1105 3 0.33840119470078633 1105 4 0.3177992536451555 1105 5 0.2363861851971123 1105 6 0.2560704455526377 1105 7 0.3821616115734159 1105 8 0.45874707045351215 1105 9 0.2662339173584911 1105 10-11 0.3781621176120069 1105 12-13 0.3994551593036917 1105 14-15 0.548188587813236 1105 16-17 0.6831339969155223 1105 18-19 0.5993415695003037 1105 20-21 0.6663416619355047 1105 22-23 0.5011000197437667 1105 24-25 0.3762432418330732 1105 26-27 0.39997010423250146 1105 28-29 0.34577997333757793 1105 30-31 0.5741084779157575 1105 32-33 0.5922086531098429 1105 34-35 0.4456743092061899 1105 36-37 0.5375058479027288 1105 38-39 0.6252578915702998 1105 40-41 0.6274647075109883 1105 42-43 0.6164860963133094 1105 44-45 0.570325655843213 1105 46-47 0.1533231681968843 1105 48-49 0.3988509454029412 1105 50-51 0.2943977082011173 1105 52-53 0.47741961819122736 1105 54-55 0.5789955946033274 1105 56-57 0.6286862631072125 1105 58-59 0.5902194622605563 1105 60-61 0.40211269931871385 1105 62-63 0.39536887765021334 1105 64-65 0.5316759429315212 1105 66-67 0.3669323872058925 1105 68-69 0.4164184806189546 1105 70-71 0.7531392625845896 1105 72-73 0.9085693307923179 1105 74-75 0.8087349019883412 1105 76-77 0.5649713585286946 1105 78-79 0.5338997653083055 1105 80-81 0.6887410301188801 1105 82-83 0.7388845647485454 1105 84-85 0.47057517795840553 1105 86-87 0.534001297959918 1105 88-89 0.5200368492181155 1105 90-91 0.35791364257781666 1105 92-93 0.25927402634548 1105 94-95 0.3531082990361014 1105 96-97 0.6118258733669535 1105 98-99 0.2935845643993318 1105 100-101 -0.3981181371267972 1106 1 0.1822507580755115 1106 2 0.2519842688234277 1106 3 0.29886767601064435 1106 4 0.19503743714840027 1106 5 0.1448508129388486 1106 6 0.07512203653194405 1106 7 0.11475969621969284 1106 8 0.14792532192092267 1106 9 0.3143556349982788 1106 10-11 0.15248710988880987 1106 12-13 0.2287847855007854 1106 14-15 0.29425902340174304 1106 16-17 0.2684398356550943 1106 18-19 0.34818000014596606 1106 20-21 0.2265795365107266 1106 22-23 0.46245775839066283 1106 24-25 0.3234026486912782 1106 26-27 0.32856602638264576 1106 28-29 0.5342028957966463 1106 30-31 0.4798916104458826 1106 32-33 0.5111567532025276 1106 34-35 0.3522019582639544 1106 36-37 0.09837239099818618 1106 38-39 0.4233981448912729 1106 40-41 0.48135102199971413 1106 42-43 0.7710072578826654 1106 44-45 0.8044900055497308 1106 46-47 0.7242808450581748 1106 48-49 0.8781388663173644 1106 50-51 0.7485327097457599 1106 52-53 1.0844152931989512 1106 54-55 0.8131012479860829 1106 56-57 0.9090724231319243 1106 58-59 1.024000741222551 1106 60-61 0.7264902093588717 1106 62-63 0.9576333200351286 1106 64-65 0.5880244137378199 1106 66-67 0.8813608703693205 1106 68-69 0.1632210756143202 1106 70-71 0.42544232097198886 1106 72-73 0.6353317651451142 1106 74-75 1.046890601710306 1106 76-77 0.7620334530606705 1106 78-79 0.9640372382771005 1106 80-81 0.13109197234933845 1106 82-83 0.720784472070136 1106 84-85 0.637848888183921 1106 86-87 0.5110680264121896 1106 88-89 0.6993680200553136 1106 90-91 0.6723050543788709 1106 92-93 0.5753954350572492 1106 94-95 0.9807419104046531 1106 96-97 0.5734848168331261 1106 98-99 0.2477859854682194 1106 100-101 0.25610645354357686 1107 1 0.06547943935781575 1107 2 0.10769073382482475 1107 3 0.08176890586321406 1107 4 0.16617587831662917 1107 5 -2.565127047233773E-4 1107 6 -0.05349253327428727 1107 7 0.07270332161118631 1107 8 0.14842235550633376 1107 9 0.1195887468053769 1107 10-11 0.15774619373775778 1107 12-13 0.11470742955649627 1107 14-15 0.3232580812786594 1107 16-17 0.1765181591852567 1107 18-19 0.18830018449653352 1107 20-21 0.2549333673869043 1107 22-23 0.16847308698792318 1107 24-25 0.16305533628834468 1107 26-27 0.24748080243743686 1107 28-29 0.2592506385206548 1107 30-31 0.18870988254121812 1107 32-33 0.26855903976071716 1107 34-35 0.18504589684771844 1107 36-37 0.20834361804985946 1107 38-39 0.18545299173261753 1107 40-41 0.18906064438659342 1107 42-43 0.12401721383204034 1107 44-45 0.1735359149531348 1107 46-47 0.08149165146461712 1107 48-49 -0.006140918776722515 1107 50-51 -0.046317187129957915 1107 52-53 0.15890972958018068 1107 54-55 0.1483773919017466 1107 56-57 -0.10896643307972198 1107 58-59 -0.18697371960294618 1107 60-61 0.04854250992159592 1107 62-63 0.17286515870291908 1107 64-65 0.2089191760427198 1107 66-67 0.013135371935277362 1107 68-69 0.11370614169025117 1107 70-71 0.02779049910963849 1107 72-73 -0.031210643125199056 1107 74-75 0.1586559366732594 1107 76-77 0.08875766562400855 1107 78-79 0.18159332006356976 1107 80-81 0.3784741433064873 1107 82-83 0.511718701693546 1107 84-85 0.4824912633526104 1107 86-87 0.6727424592286297 1107 88-89 0.6021134176567138 1107 90-91 0.4330378733639222 1107 92-93 0.2006178524941653 1107 94-95 0.09402329965426759 1107 96-97 0.4242204367246529 1107 98-99 0.36476202498646515 1107 100-101 0.3986114824438367 1108 1 0.13456588256454793 1108 2 0.141289359867649 1108 3 0.15608060800618517 1108 4 0.21013962964189403 1108 5 0.10114958662892803 1108 6 0.05559026395668809 1108 7 0.13224018674366533 1108 8 0.1536629659194375 1108 9 0.1816317405352521 1108 10-11 0.24619948447074336 1108 12-13 0.20231878773044087 1108 14-15 0.24184728055521987 1108 16-17 0.3460392410805966 1108 18-19 0.23598379055844987 1108 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-1.01552639184386 2316 72-73 -1.0220220063487844 2316 74-75 -0.7216514295068812 2316 76-77 -0.7605609801070123 2316 78-79 -0.6486278885281536 2316 80-81 -1.0030607539052951 2316 82-83 -0.7078331523436674 2316 84-85 -0.6792347076257883 2316 86-87 -0.6446427596170672 2316 88-89 -0.5218554698306015 2316 90-91 -0.5236348443635777 2316 92-93 -0.604021284104693 2316 94-95 -0.5809272060415296 2316 96-97 -0.23453226162981267 2316 98-99 -0.271590603972065 2316 100-101 -0.1413481088791393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 15.0 5 31.0 6 80.0 7 197.0 8 454.0 9 749.0 10 1345.0 11 2237.0 12 3100.0 13 4481.0 14 5594.0 15 7418.0 16 9471.0 17 12265.0 18 11070.0 19 8380.0 20 7602.0 21 7703.0 22 8362.0 23 9456.0 24 10621.0 25 12412.0 26 14860.0 27 17731.0 28 21417.0 29 25625.0 30 31474.0 31 38028.0 32 45277.0 33 56191.0 34 71127.0 35 93536.0 36 130544.0 37 180584.0 38 118479.0 39 6240.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.34834328042589 21.335538323509425 15.550210746966087 22.7659076490986 2 15.323915019488824 22.277034001448182 40.10281060150878 22.296240377554216 3 18.091763536846255 25.685105876266224 31.877645880125975 24.345484706761546 4 12.353361446327312 19.14893180203744 38.19326109361463 30.304445658020622 5 12.36517157552755 38.53529427478799 35.764132197099684 13.33540195258478 6 29.63361405266464 38.36900152335747 18.27844182293741 13.718942601040482 7 25.958154889731095 34.0069023285607 22.213302893557962 17.82163988815025 8 24.14973248857482 36.577208765669845 21.151982623049097 18.12107612270624 9 24.906894526999558 16.819652543103626 21.94538085989628 36.32807207000053 10-11 23.056230778390496 27.91490960454297 29.22480198406018 19.80405763300635 12-13 24.195207377813183 25.847444578120317 28.907401628473234 21.04994641559327 14-15 21.10994657983666 27.80265951112954 27.691692569218322 23.39570133981547 16-17 19.95890835629319 30.39303443773328 28.922748120439678 20.72530908553385 18-19 19.5839714873471 28.99963455845217 31.396561564582555 20.019832389618173 20-21 21.076122706238376 28.068015241786803 31.29919602859477 19.55666602338005 22-23 20.218874850641168 28.71046353591387 30.78111077806201 20.289550835382954 24-25 19.899462307090605 28.49998254910374 31.51626731488192 20.08428782892373 26-27 19.975166425500046 28.759671260552857 31.169883105623793 20.0952792083233 28-29 19.668608160432946 28.912688212662346 31.211428465843532 20.207275161061176 30-31 20.33754070156565 28.60945384965981 31.269940174344363 19.78306527443018 32-33 19.744211446943225 28.69075432883991 31.229546565054754 20.335487659162112 34-35 20.176301769331417 28.95781970159183 31.1010467949636 19.764831734113155 36-37 20.169396528715904 28.718881009768378 30.807902981428175 20.303819480087544 38-39 19.91539103927254 28.791170774697587 31.09959312349449 20.193845062535384 40-41 20.572827643553378 28.403833222976754 31.011174245340644 20.012164888129224 42-43 20.203754925394737 28.512909320384068 30.873197999713597 20.4101377545076 44-45 20.410896117540844 28.67557653309737 30.671750686614725 20.241776662747064 46-47 20.400671755474438 28.563055091339855 30.15477886680272 20.88149428638299 48-49 20.321886253238674 28.583893471735767 30.29530961932488 20.798910655700684 50-51 20.436959279956966 28.865416911320885 29.78302421358211 20.91459959514004 52-53 20.32368266534177 28.949591649865937 29.879773836848827 20.84695184794347 54-55 20.543050246159783 28.76353468204532 29.618113582517935 21.07530148927696 56-57 20.64518910573579 28.93753002574515 29.24718014625874 21.17010072226032 58-59 20.352527911111476 28.95267121347124 29.449712779367744 21.245088096049535 60-61 20.49824259570257 28.804595530116078 29.404494520429825 21.292667353751522 62-63 20.18972164851093 28.760095836019396 29.479738524519483 21.57044399095019 64-65 20.260756915673337 28.757221576654445 29.2415856057091 21.74043590196312 66-67 20.178542924262715 28.835251987987643 29.141514745728003 21.844690342021643 68-69 20.34056112105133 28.574706130531318 29.15187182353563 21.932860924881716 70-71 20.03088805466847 28.261748803844945 29.26024700178512 22.447116139701468 72-73 20.002689485548636 28.17918748793838 29.305794506879746 22.512328519633247 74-75 19.991551730509443 28.162249888109187 29.056965767570965 22.789232613810405 76-77 19.712132659387947 28.034191368188523 29.449610127247567 22.804065845175966 78-79 19.63386041775307 27.93682583220074 29.308155505643814 23.12115824440238 80-81 19.458708980370115 28.003007742627567 29.213855120219883 23.324428156782435 82-83 19.529666101734932 27.941588590774195 29.38827234568484 23.14047296180603 84-85 19.587365347866637 27.722261031761146 29.277750773638644 23.412622846733573 86-87 19.17709502626145 27.803210297424464 29.20607937307171 23.81361530324238 88-89 19.528703343945857 27.428403958883703 29.207776723311845 23.8351159738586 90-91 19.385398242352508 27.340236495052572 29.24781063971101 24.026554622883907 92-93 19.11257630956817 27.237447351915556 29.33926461545726 24.310711723059015 94-95 18.97190822079239 27.242024956783457 29.278386390792516 24.507680431631634 96-97 18.967288875384433 27.123872366459857 29.147196981206452 24.76164177694926 98-99 19.016356588828987 26.827156413088556 29.165520384658024 24.990966613424433 100-101 18.85154860988499 26.803135816967167 29.289934754312412 25.05538081883543 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 408.0 1 234.0 2 71.0 3 83.5 4 76.0 5 85.0 6 147.5 7 253.5 8 358.0 9 435.5 10 465.5 11 459.5 12 510.0 13 599.0 14 697.0 15 839.0 16 1003.0 17 1194.0 18 1398.5 19 1653.0 20 1957.0 21 2351.5 22 2915.0 23 3596.5 24 4383.0 25 5518.5 26 7168.5 27 9209.5 28 11241.5 29 13463.0 30 16043.5 31 18728.0 32 21622.0 33 24403.5 34 27340.5 35 30627.0 36 33702.5 37 35968.5 38 36722.0 39 37047.0 40 37651.5 41 37932.0 42 37978.5 43 38330.5 44 38995.0 45 39370.0 46 39463.5 47 39446.0 48 39028.0 49 37536.0 50 35585.0 51 32982.0 52 29655.0 53 26418.0 54 23046.0 55 19665.5 56 16222.5 57 12936.5 58 10115.5 59 7772.0 60 5798.0 61 4148.0 62 2921.0 63 2047.5 64 1407.0 65 928.0 66 599.5 67 407.0 68 287.5 69 209.5 70 146.5 71 85.0 72 68.5 73 60.5 74 49.0 75 30.5 76 16.5 77 11.0 78 11.0 79 11.0 80 7.0 81 4.5 82 3.0 83 1.0 84 0.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0010265212017689014 2 0.054302971573574874 3 8.212169614151211E-4 4 0.0 5 0.09136038695743222 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.0530424035378027E-4 26-27 0.008160843554062765 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009803277476893009 36-37 0.0 38-39 0.0036441502662795996 40-41 0.005029953888667617 42-43 3.592824206191155E-4 44-45 5.1326060088445066E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.1326060088445066E-5 68-69 7.18564841238231E-4 70-71 1.0265212017689013E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0011804993820342365 82-83 5.132606008844507E-4 84-85 0.0034388460259258194 86-87 0.0017964121030955775 88-89 5.1326060088445066E-5 90-91 0.002155694523714693 92-93 7.18564841238231E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 974164.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.3945380360165 #Duplication Level Percentage of deduplicated Percentage of total 1 77.13708601575331 52.75755363491916 2 14.264181460481188 19.51184202903044 3 4.663258325447125 9.56824196734692 4 1.8711633465300126 5.119094107033875 5 0.8685559514836667 2.9702241530079068 6 0.44073729664576616 1.8086414279597969 7 0.24448615386011457 1.1705062284625491 8 0.1477442391477825 0.8083919187196263 9 0.0871106893946243 0.5362105823229826 >10 0.25539137206841894 2.733390177101826 >50 0.011122556808210957 0.5214448891315575 >100 0.00796094157073101 0.9063103661782029 >500 4.506190533909878E-4 0.1955007847369256 >1k 7.510317556516464E-4 1.3926477340482204 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4380 0.44961628637477874 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4149 0.42590364661391716 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2680 0.27510768207406555 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1330 0.13652731983526387 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1017 0.10439720621989727 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.0265212017689013E-4 0.0 0.0 0.0 5 0.0 1.0265212017689013E-4 0.0 0.0 0.0 6 0.0 1.0265212017689013E-4 0.0 0.0 0.0 7 0.0 1.0265212017689013E-4 0.0 0.0 0.0 8 0.0 1.0265212017689013E-4 0.0 0.0 0.0 9 0.0 1.0265212017689013E-4 0.0 0.0 0.0 10-11 0.0 1.0265212017689013E-4 0.0 0.0 0.0 12-13 0.0 1.0265212017689013E-4 0.0 0.0 0.0 14-15 0.0 1.0265212017689013E-4 0.0 0.0 0.0 16-17 0.0 1.0265212017689013E-4 0.0 0.0 0.0 18-19 0.0 1.0265212017689013E-4 0.0 0.0 0.0 20-21 0.0 1.0265212017689013E-4 0.0 1.0265212017689013E-4 0.0 22-23 0.0 1.0265212017689013E-4 0.0 8.725430215035661E-4 0.0 24-25 0.0 1.0265212017689013E-4 0.0 0.002463650884245363 0.0 26-27 0.0 1.0265212017689013E-4 0.0 0.006261779330790298 0.0 28-29 0.0 1.0265212017689013E-4 0.0 0.016116382867771752 0.0 30-31 0.0 1.0265212017689013E-4 0.0 0.045885497719069884 0.0 32-33 0.0 1.0265212017689013E-4 0.0 0.10706616134449641 0.0 34-35 0.0 1.0265212017689013E-4 0.0 0.21767382083509554 0.0 36-37 0.0 1.0265212017689013E-4 0.0 0.38602329792519535 0.0 38-39 0.0 1.0265212017689013E-4 0.0 0.593791189163221 0.0 40-41 0.0 1.0265212017689013E-4 0.0 0.8067430124701795 0.0 42-43 0.0 1.0265212017689013E-4 0.0 1.021542573940322 0.0 44-45 0.0 1.0265212017689013E-4 0.0 1.3087118801351725 0.0 46-47 0.0 1.0265212017689013E-4 0.0 1.6274980393445047 0.0 48-49 0.0 1.0265212017689013E-4 0.0 1.947670002176225 0.0 50-51 0.0 1.0265212017689013E-4 0.0 2.2615288596170666 0.0 52-53 0.0 1.0265212017689013E-4 0.0 2.571127654070567 0.0 54-55 0.0 1.0265212017689013E-4 0.0 2.9308720092304785 0.0 56-57 0.0 1.0265212017689013E-4 0.0 3.352823549217586 0.0 58-59 0.0 1.0265212017689013E-4 0.0 3.79037821147158 0.0 60-61 0.0 1.0265212017689013E-4 0.0 4.2284974603865475 0.0 62-63 0.0 1.0265212017689013E-4 0.0 4.670722794108589 0.0 64-65 0.0 1.0265212017689013E-4 0.0 5.151186042596524 0.0 66-67 0.0 1.0265212017689013E-4 0.0 5.696679409216518 0.0 68-69 0.0 1.0265212017689013E-4 0.0 6.29637309528991 0.0 70-71 0.0 1.0265212017689013E-4 0.0 6.922705006549205 0.0 72-73 0.0 1.0265212017689013E-4 0.0 7.525118973807285 0.0 74-75 0.0 1.0265212017689013E-4 0.0 8.158585207418874 0.0 76-77 0.0 1.0265212017689013E-4 0.0 8.862265491231456 0.0 78-79 0.0 1.0265212017689013E-4 0.0 9.633439544060343 0.0 80-81 0.0 1.0265212017689013E-4 0.0 10.427658997868942 0.0 82-83 0.0 1.0265212017689013E-4 0.0 11.222135081977983 0.0 84-85 0.0 1.0265212017689013E-4 0.0 12.02764626900604 0.0 86-87 0.0 1.0265212017689013E-4 0.0 12.887409101547583 0.0 88-89 0.0 1.0265212017689013E-4 0.0 13.792852127567842 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2300 0.0 48.612885 1 ATTACCG 45 9.582677E-4 42.259464 5 GTATCAA 3930 0.0 38.37762 1 TATCAAC 4935 0.0 30.465706 2 ATCAACG 4925 0.0 30.117912 3 GTATACG 95 3.451146E-5 30.04962 1 TCAACGC 4955 0.0 29.935564 4 CAACGCA 4965 0.0 29.875273 5 AACGCAG 5050 0.0 29.343775 6 ACGCAGA 5745 0.0 25.793919 7 CGCAGAG 5755 0.0 25.7491 8 GCAGAGT 6360 0.0 22.851625 9 TAGGACG 420 0.0 22.638998 4 GTACATG 3440 0.0 22.267874 1 GGACGTG 435 0.0 21.837027 6 AGTACTT 4320 0.0 21.60387 12-13 GTGGTAT 1055 0.0 21.196138 1 GAGTACT 4250 0.0 21.065666 12-13 TACATGG 3620 0.0 20.897768 2 AGAGTAC 5945 0.0 20.691916 10-11 >>END_MODULE